RU2279681C2 - Method for quick-test detection of urinary calcium content - Google Patents

Abstract

FIELD: medicine, laboratory diagnostics.

SUBSTANCE: the present innovation deals with laboratory diagnostics of pathological states associated with calcium exchange disorders, the loss of bony mass, traumatic disease and so on. One should prepare saturated solution of oxalic acid, then it is necessary to introduce by drops the tested urine at mixing into 1 ml prepared solution to visualize the turbidity. Calcium concentration is considered to be above the norm in case when solution's turbidity occurs at adding about 1-7 urinary drops. Application of the present innovation enables to detect urinary calcium content within short period of time under domestic conditions at applying available and harmless substances.

EFFECT: higher efficiency.

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Description

The invention relates to medicine, in particular to laboratory diagnosis of pathological conditions associated with impaired calcium metabolism, bone loss, with traumatic disease, etc., and is intended for use at home.

Currently, in laboratory diagnostics, many chemical methods for determining calcium in urine are used. But their use is possible only in a clinical diagnostic laboratory, since research requires special training and the availability of laboratory equipment.

Of the known methods for determining calcium in urine, the simplest method of determination is the Sulkovich test, which is made by mixing urine and Sulkovich's reagent, determining the degree of turbidity and an approximate estimate of the calcium content in urine (Laboratory research methods in the clinic: Reference book / V. Menshikov, Delektorskaya L.N., Zolotnitskaya R.P., et al .; Edited by V.V. Menshikov. - M .: Medicine, 1987. - 368 p.).

However, laboratory glassware and chemical reagents (ammonium oxalate, glacial acetic acid), which are poisonous and potent substances, to which only specialists are allowed to work, are necessary with this method. In this regard, the implementation of this analysis at home is impossible.

The objective of the present invention was to develop the simplest method for studying the calcium content in urine, which would allow to determine it at home, when the availability and safety of the substances used, as well as the speed of the analysis, are of great importance.

The problem is solved in that in a method for the rapid determination of calcium in the urine, including the use of oxalic acid and visualization of the turbidity of the solution, a saturated solution of oxalic acid is prepared, then the test urine is added dropwise with stirring with stirring and the turbidity is visualized, if clouding is observed solution occurs when adding from 1 to 7 drops of urine, then the concentration of calcium is higher than normal.

The proposed method is illustrated by a detailed description, an example implementation.

The method is as follows.

At home, a saturated solution of oxalic acid at room temperature is prepared. To prepare the solution, 2 teaspoons of oxalic acid are added to the glass in a dry form, 50 ml (a quarter cup) of distilled water are slowly added and mixed, while a small amount of undissolved substance is retained at the bottom of the glass.

Then measure with a syringe in a penicillin vial 1 ml of the prepared solution of oxalic acid. Add the test (morning) urine to the eye dropper dropwise into it until the appearance of turbidity. After adding each drop, the contents of the vial are gently mixed. If before the appearance of turbidity, 1-7 drops of urine were added to the solution, then the concentration of calcium is higher than the permissible norm.

An example of the method.

The calcium content in the urine samples of patients Ch. And C., measured by the standardized method by reaction with calcein, was 6.76 mmol / L and 3.04 mmol / L, respectively, with an upper limit of normal - 3.93 mmol / L. We measured with a syringe in two penicillin vials of 1 ml of a saturated solution of oxalic acid. The test (morning) urine was added dropwise with an eye dropper until the appearance of turbidity. After adding each drop, the contents of the vial were gently mixed. Before the appearance of turbidity, 6 drops of patient C.'s urine were added to the first bottle of oxalic acid solution, and 12 drops of patient C's urine to the second one. Based on the data obtained, it was concluded that the calcium content in patient C.'s urine was higher than normal, and patient C. calcium content is within normal limits.

The proposed method allows patients to adjust the dose of biological additives, as well as promptly contact the clinic for advice on the results obtained with an independent study. This would allow the clinic to further outline a differentiated scheme for examination aimed at assessing the intensity of the pathological process, as well as reduce the load on the work of the clinical diagnostic laboratory.

The proposed method is tested in the clinical units of the State institution RRC "WTO" them. Academician G.A. Ilizaroz.

Claims (1)

A method for the rapid determination of calcium in urine, including the use of oxalic acid and visualization of the turbidity of the solution, characterized in that a saturated solution of oxalic acid is prepared, then the test urine is added dropwise with stirring in 1 ml of the prepared solution, if the solution becomes cloudy with the addition of 1 up to 7 drops of urine, then the concentration of calcium is above normal.