RU2350101C2 - "lacto-plus" farm animal fodder additive - Google Patents

Abstract

FIELD: agriculture.

SUBSTANCE: proposed fodder additive consists of a lactobacilli mixture (including specimens of Lactobacillus acidophilus, Lactobacillus plantarum, Lactobacillus bulgaricus, Lactobacillus helveticus, Lactobacillus brevis, Lactobacillus fermentum, Lactobacillus casei, Lactococcus lactis, Streptococcus cremoris, Streptococcus diacetilactis, Streptococcus salivarius, Leuconostoc citrovorum and Leuconostoc dextranicum) and a yeast fungi mixture (including specimens of Saccharomyces cerevisiae, Saccharomyces unisporus, Torulopsis sphaerica, Torulaspora delbrueskii, Candida kefir, Candida holmii, Candida friedrichii, Kluyveromyces lactis, Kluyveromyces marxianus) in the ratio of 3:1.

EFFECT: invention enables production of a fodder additive characterised by antimicrobial and prophylactic properties, its application providing for improved animal productivity and BWG.

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Description

The invention relates to the field of applied biotechnology and can be used for the preparation of feed additives used in the feeding of farm animals and poultry.

The known probiotic lactomycrocyclic used for growing and feeding broiler birds (RU 2268925 C2, C12N 1/20, AK61 35/74, 2006.01.27), characterized in that it contains microbial biomass of strains of Lactobacillus amylovoms VKPM B-6253 and Escherichia coli VKPM B-6253 and Escherichia coli B-8564 taken in a 1: 1 ratio.

This probiotic allows you to increase the safety of young animals, increase productivity and improve the quality of broiler chicken meat. The main disadvantage is the limited scope of use, as it is used only in poultry farming.

A known method of obtaining a biologically active feed additive (RU 2223314 C2, C12N 1/20, AK61/35/66, 2004.02.10), intended for use as a prophylactic for gastrointestinal infections of calves and stimulate their growth and development and containing 1 g viable bifidobacteria cells up to 10 ¹² , acidophilus bacillus - up to 10 ⁹ , ruminococci - up to 10.

Obtained in the known method, the feed additive also has a limited area of use.

The closest to the claimed solution for the composition of bacteria included in the feed additive and the effect on animals is a feed additive based on a mixture of an antimicrobial preparation and a normalizing component, containing a mixture of live lactic acid bacteria and dicytroboratoxyquinoline in a ratio of 5-20: 1 by dry weight (RU 2136173 C1 , A23K 1/16, 1999.09.10).

A disadvantage of the known feed additive is the limited spectrum of its action: this additive provides effective antimicrobial protection, but at the same time it does not increase productivity and increase live weight, nor does it have the necessary effect on the digestion of animals.

The objective of the present invention is to provide a feed additive for farm animals with a complex effect, used both as a prophylactic to prevent diseases of young farm animals, and to increase animal productivity and increase live weight.

The objective of the invention is solved in that a feed additive for farm animals, including a mixture of lactic acid bacteria, is characterized in that the mixture of lactic acid bacteria is represented by bacteria of the species Lactobacillus acidophilus, Lactobacillus plantarum, Lactobacillus bulgaricus, Lactobacillus helveticus, Lactobacillus brevisobactus lactobus ferlactobactus Streptococcus cremoris Streptococcus diacetilactis Streptococcus salivarius Leuconostoc citrovorum Leuconostoc dextranicum holding a mixture of lactic acid bacteria and yeasts mixture in a ratio of 3: 1.

Distinctive features of the proposed solutions are:

- the presence in the feed additive of a mixture of lactic acid bacteria represented by the species Lactobacillus acidophilus, Lactobacillus plantarum, Lactobacillus bulgaricus, Lactobacillus helveticus, Lactobacillus brevis, Lactobacillus fermentum, Lactobacillus casei, Lactococococcus lactis. Streptococcus diacetilactis, Streptococcus salivarius, Leuconostoc citrovorum, Leuconostoc dextranicum;

- additional inclusion in the feed additive of a mixture of yeast species of the species Saccharomyces cerevisiae, Saccharomyces unisporus, Torulopsis sphaerica, Torulaspora delbrueskii, Candida kefir, Candida holmii, Candida friedrichii, Kluyveromyces lactis, Kluyveromyces marxianus;

- the content of a mixture of lactic acid bacteria and a mixture of yeast in a ratio of 3: 1.

The technical result of the invention is expressed in improving digestion and better absorption of feed by animals and is achieved due to the fact that the lactic acid bacteria contained in the inventive feed additive contribute to the suppression of pathogenic microflora, the production of hydrolytic enzymes that break down proteins, fats, carbohydrates, the decomposition of starch and cellulose with the formation of monosaccharides and yeast fungi improve the secretion of the stomach and pancreas, enhance the absorption of nutrients in the intestines and increase resistance Bridges to infection. Improving the assimilation of animal feed leads to an increase in milk productivity, an increase in live weight of animals, and the safety of young animals.

The content of a mixture of lactic acid bacteria and a mixture of yeast in a ratio of 3: 1 allows for the most effective prevention of animals from diseases and stimulate the processes in the digestive tract, in particular cellulolytic processes.

The following examples show a method for producing the inventive feed additive, and also reflects the results of scientific and economic experiments conducted to study the effect of the feed additive on farm animals.

Example 1. In a glass bottle with a capacity of 10 l pour 1 l of barley dermat containing 2.5 · 10 ⁸ yeast cells defined as Saccharomyces cerevisiae, Saccharomyces unisporus, Torulopsis sphaerica, Torulaspora delbrueskii, Candida kefir, Candida holmii, Candida friedrichii, lactis, Kluyveromyces marxianus) and diluted with warm boiled water (18-20 ° C). The resulting aqueous solution barley Dirty administered finished mixture of lactic acid bacteria species Lactobacillus acidophilus, Lactobacillus plantarum, Lactobacillus bulgaricus, Lactobacillus helveticus, Lactobacillus brevis, Lactobacillus fermentum, Lactobacillus casei, Lactococcus lactis, Streptococcus cremoris, Streptococcus diacetilactis, Streptococcus salivarius, Leuconostoc citrovorum, Leuconostoc dextranicum in the amount of 1 g of bacteria dissolved in 100 ml of warm boiled water (total number of microbial cells up to 7.5 · 10 ⁸ ). The bottle is topped up with warm boiled water, not reaching a stopper of 1-1.5 cm. The glass bottle is sealed with a cotton-gauze stopper and placed in a thermostat for 14 days, where the temperature is maintained at 34-35 ° С. Once within 2-3 days, the bottle is removed from the thermostat, the lid is uncorked in order to get air and feed aerobes, its contents are shaken to mix the grown microorganisms.

After 14 days, a bottle with grown microorganisms, including lactic acid bacteria and yeast, is removed from the thermostat, its contents are filtered through paper filters, diluted with warm boiled water in a ratio of 1:10 and poured into a glass or plastic container.

The finished supplement is fed to animals.

Example 2

Scientific and economic experience was carried out at Tayozhniy Open Joint-Stock Company OJSC in the Sukhobuzim district of the Krasnoyarsk Territory on sexually mature black-motley cows, formed in two groups of 30 animals in each group. The main diet of cows (OR) consisted of bonfire hay - 5.1 kg, cereal and bean hay - 3.9 kg, grain mixtures - 8.5 kg, soybean meal - 0.2 kg, table salt - 0.15 kg. The diet contained: feed units - 20.41; dry matter - 25.6 kg, digestible protein - 2199 g, crude fiber - 5.6 kg. The content of vitamins, macro- and microelements in the diet of experimental groups of animals was within the physiological norm. The results of the experiment, shown in table 1, show that the inclusion of the Lacto-Plus feed additive in the main diet had a positive effect on milk productivity: milk yields in the first 100 days of lactation in the cows of the experimental group were 282 higher on average compared to the control group , 1 kg, taking into account the conversion to the basic fat content of milk (3.4%). The economic effect of feeding Lacto-Plus supplements to cows was 3,051.6 rubles.

Clinical indicators of dairy cows of the control and experimental groups according to body temperature, pulse rate and respiration, scar reduction were within the physiological norm. The movement of the scar by the number of contractions in 5 minutes in the experimental group was 1 reduction higher compared to the control group, which indicates the positive effect of the feed additive for farm animals "Lacto-Plus" on the motility of animal rumen.

Example 3

Scientific and economic experience was carried out in ZAO Elita of the Emelyanovsk district of the Krasnoyarsk Territory on fattening young pigs of large white breed.

Young animals at the age of 3.5 months are formed in three groups of 12 goals each. The duration of the experiment was 154 days. The experimental animals were kept in groups in the same building under identical microclimatic conditions.

The main ration (OR) of animals had the following composition,%: grain mix - 91; feed yeast - 5.5; monosodium phosphate - 0.5; chalk - 1.5; table salt - 0.5; premix - 1%. The Lacto-Plus feed additive for the animals of the experimental groups was introduced into a warm mixer (t = 37-38 ° C). The resulting mass was kept for 6-7 hours in a warm room.

The feeding results are shown in table 2, from which it is seen that the inclusion of the Lacto-Plus feed additive in the diet of fattening young pigs had a positive effect on the increase in live weight: the average daily increase in the experimental groups is higher than in the control, respectively, by 20.1 g and 24 g. The cost of growth in live weight in the experimental groups decreased by 48.4 and 82.4 rubles. Animals in the experimental groups showed better results in terms of blood hemoglobin, they digested food faster, were more active than animals in the control group.

Thus, the inventive feed additive for farm animals "Lacto-Plus" has a complex effect and, having good antibacterial and preventive properties, due to the presence of a complex of lactic acid bacteria in it, at the same time it improves the productivity of animals due to improved digestibility of feed and digestion processes due to a complex of yeast fungi.

Table 1 The test results of the feed additive for farm animals "Lacto-Plus" on sexually mature cows of black-motley breed, in OJSC PZ "Tayozhniy" Sukhobuzim district of Krasnoyarsk Territory Indicator Group of cows 1 - control 2 - experienced Number of animals thirty thirty Feeding pattern OR (main diet) OR + "Lacto-Plus" (50 ml / goal. Per day) The duration of the experiment, days one hundred one hundred Milk yield per 100 days of lactation, kg 2806,2 3088.3 Total costs for 1 cow, rub. 7602 8102 including Lacto Plus - 500 Cost of 1 kg of milk of basic fat content, rub. 2.71 2.62 Selling price of 1 kg of milk, rub. 12.59 12.59 Revenue from the sale of milk, rubles. 35330,1 38881.7 Profit RUB 27728.1 30779.7 table 2 The test results of the feed additive for farm animals "Lacto-Plus" on fattening young pigs of large white breed in ZAO "Elite" Emelyanovsky district of the Krasnoyarsk Territory Indicator Group 1 - control 2 - experienced 3 - experienced Number of animals 56 56 56 Feeding pattern OP (main ration) OR + Lacto-Plus (3 g / goal) OR + Lacto-Plus (6 g / goal) The duration of the experiment, days 154 154 154 Blood hemoglobin, g% 10.5 10.7 10.6 Reserve alkalinity, mg% 467.8 479.5 476.5 The digestibility of protein,% 70.3 73,4 74.1 The average daily gain in live weight of pigs, g 507.8 527.9 531.8 Gross increase in live weight, kg 78,2 81.3 81.9 Costs for the period of the experiment, rub. 2951.0 3001.0 3051.0 including Lacto Plus - 50,0 100.0 Cost of 1 centner growth, rub. 3,773.7 3691.3 3725.3

Claims (1)

A feed additive for farm animals, including a mixture of lactic acid bacteria, characterized in that the mixture of lactic acid bacteria is represented by bacteria of the species Lactobacillus acidophilus, Lactobacillus plantarum, Lactobacillus bulgaricus, Lactobacillus helveticus, Lactobacillus lactobacillococactus lactobacillus breum, breactum diacetilactis, Streptococcus salivarius, Leuconostoc citrovorum, Leuconostoc dextranicum and includes a mixture of yeast species of the species Saccharomyces cerevisiae, Saccharomyces unisporus, Torulopsis sphaerica, Torulaspora delbrueskii, Candida kefirii, Candida franliida kluida holding a mixture of lactic acid bacteria and yeasts mixture in a ratio of 3: 1.