RU2347211C2 - Laser-luminescent sensor for concentration measurement with contact maker of bundle for minute measuring of terrain clearance concentration of luminescents and method of its use - Google Patents

Abstract

FIELD: physics; measuring.

SUBSTANCE: invention concerns the measuring technics. The device contains laser, spectrometer, system of optic cables with the core combined optic cable and the computer with programs for flashing, storing and printing of diagrammes of a spectral intensity distribution. The device is supplied by the contact maker of a bundle which contains the case with resorts for embedding between the laser and laser optic cable so each carrier of test is supplied by the safety cover preventing excitation of phosphorescents from casual illumination with the same reflective properties as at the carrier.

EFFECT: almost simultaneous measuring of all luminescents in the test.

11 cl, 8 dwg

Description

The group of proposed inventions relates to physics, measurements, research of materials by determining their chemical or physical properties using optical means; to systems in which the material is excited by optical means, and it fluoresces and phosphoresces, namely to measure the concentration of luminescence by laser-luminescent concentrometers in various biotechnological processes.

In the description - only the basic information for a quick visual understanding of the essence, the rest of the information known from the prior art and logically following from them and this description may not be mentioned, but it means, but the actions are performed.

As the first analogue of the group device, the first known laser-fluorescence concentmeter containing laser 1 was chosen (position numbers were taken from Fig.1 (c) of the Appendix to this description), spectrometer 5, 6; The U-shaped system of light cables 2, 4, optically connecting them during measurement through sample 3; a computer 7 ... 9 with computer programs, a set of training samples and the basis for the placement of its parts [1]. This concentrator is the founder of a number of developed under the guidance of prof. MT Aleksandrova of laser-luminescent concentrometers, selected as analogues in the application, “Fiber cable” (hereinafter briefly “cable” because there are no electrical cables in the application) is a fiber-optic cable containing fibers of total internal reflection in the range of operating frequencies of the concentrator (frequency laser and frequencies excited by its beam of luminescents). “Luminescent” - a phosphor that can luminesce in the frequency range of the spectrometer, respectively: “fluorescent” and “phosphorescent”, as well as their derivatives. The “U-shaped system of light cables” (named for clarity, see the callout in Fig. 1 (c) in the Appendix) contains a laser cable 2 (right branch in the letter “U”, Fig. 1 (c) and Fig. 1, the diameters are exaggerated) with a laser axial fiber 11, a spectro cable 4 (right branch “U”) with 6 axisymmetric spectral fibers 12 and a combined cable 13 (lower branch “U”) with an axial laser fiber 11a and 6 axisymmetric spectral fibers 12a, sequentially connected to the respective fibers 11 and 12 of said cables 2 and 4 with a U-shaped optical connector 14; the ends of all fibers and cables are perpendicular to their geometric axes in an unbent position. “Sample” means a substance to be measured, usually found in a test medium (not shown), for example in a test tube. Computer programs provide highlighting on a computer screen 7 ... 9, storing and printing graphs of the spectral distribution of flows entered into the spectrometer (briefly: “spectrographics”), with curves of the spectral distribution of these flows (“curve”) and mathematical operations on them. A set of tuning samples in the form of plates with desired optical properties is intended for tuning the power of the laser beam (“beam”), checking the spectrometer, identifying the causes of errors and eliminating them, etc.

Description of the 1st analogue of the device. During the measurement, the beam illuminates the input end of the laser fiber 11, passes through the axial fibers 11 and 11a of the laser 2 and the combined 13 cables, and illuminates the carrier with sample 3 (hereinafter, an example of a carrier in the form of a tube). Some parts of the beam are reflected from the carrier and the sample, including from the molecules in the volume, the other part excites the fluorescence of bacteria in the sample (“bacteria” is the generalized name for all microorganisms measured by a concentration meter). The fractions of the reflected and fluorescence fluxes illuminate the input ends of the spectral fibers 12a (“spectroscopes”), but the fractions of those fractions that have 90 ° incidence angles or are very close to it will pass into the spectrometer, because only they will have an incidence angle on the inner surface of the fiber greater than the limiting angle full internal reflection. This is important, because when measuring, values are compared under the same conditions. Unambiguous conditions for flows are the surface density of the flow incident on a plate perpendicular to its rays, i.e. intensity, and the fiber automatically provides this condition. This ensures accurate measurement of flows by direct measurements, and the concentration is measured by indirect methods, where other causes of errors. The spectrometer decomposes all intensities in the form of a fan, which gives the spectral band where the photoelectric converters are located, their signals, the electric power of which is proportional to the intensity of the illuminated streams, are fed to the computer. He highlights them in the form of dashes over the spectral (differential) section of the abscissa axis at the height of the corresponding value of the intensity of the ordinate axis scale. It turns out a step ladder of short steps of various heights, which the eye sees as a curve. A number of converters give signals not at the same time, but in bursts, like a multi-barreled high-speed gun, therefore a 2-stroke mode is obtained: the highlight cycle and the break cycle, and the eye sees flickering curves, which often do not coincide due to errors during measurement. The curve is mathematically written as: I = f (λ), where I is the intensity, λ is the wavelength. Reflected lobes of the beam highlight the peak: I _p = f _p (λ) = P (designation of the peak of the curve), the proportion of the fluorescent flux illuminates the hill: I _x = f _x (λ) = X (designation of the hill, as part of the curve). Form X depends on the type of bacteria, the height and area under the curve depends on the concentration. The computer calculates the integral intensity of peak P by the formula (I):

where “n” and “k” are the beginning and end of the area of integration. The computer calculates the integral flow of hill X by the formula (2):

The computer calculates the coefficient (excitation) of fluorescence according to the formula (3):

To _f take for the concentration of bacteria in arbitrary units. An analogue was developed in medicine to detect and measure the concentration of bacteria in arbitrary units; under the action of a red laser, they fluoresce in the near infrared region; therefore, an appropriate spectrometer was chosen for this region, but this does not mean that there will be no X from the phosphorescent flux in this region if phosphorescent appears in the sample, moreover, other luminescents can be measured in other areas, if the appropriate laser and spectrometer are selected. The advantages and disadvantages of the 1st analogue of the concentrator are clearly shown in the method, because they are created for each other, so they will be indicated after the description of the method.

As the first analogue of the method, a method for measuring the fluorescence concentration was chosen, in which they try to manually correctly rest the working end of the combined cable 13 of the tuned laser fluorescence concentration meter in the carrier with sample 3, the types of bacteria in sample 3 are determined by form X, the highlighted value of K _{f is} taken as the concentration bacteria in arbitrary units [1]. This method is the founder of a number of developed under the guidance of prof. M.T.Alexandrova laser-luminescent methods for measuring the concentration of luminescence selected in the application as analogues. “Correctly abut” as applied to a sample in a test tube as a carrier (this most often happens) means that the working end of the combined cable 13 (hereinafter briefly referred to as “working end”) with the center of the end of the laser fiber 11a is motionlessly abutted into the generatrix of the cylindrical surface of the tube, therefore illuminates the largest sample volume; the largest reflected fraction of the beam and fluorescence flux enters the spectrometer. This increases the intensity values compared to the errors. But it’s impossible to correctly support it manually, so with each measure the curve takes a different look, and the computer prints the averaged data. During preparation for the measurement, a carrier with a sample is prepared in advance, the concentmeter is heated, the beam power is adjusted using tuning samples, the spectrometer is checked, contaminants are detected and eliminated on the carrier, at the ends of the fibers at the working end, on the samples, so it takes less than a minute to measure what the doctor makes a diagnosis.

Advantages of the 1st group of analogues:

1. The unprecedented brevity of measurement, because it is based on the phenomenon of fluorescence. This allows you to conduct continuous diagnosis of the patient, because the speed of the processes in his body is much lower, moreover, fluorescence does not interfere (errors) with each next light cycle.

2. A significant increase in the number of analyzes performed by the laboratory with such a concentrator, which increases the cost-effectiveness of the treatment while improving the quality.

3. The light cable only measures intensities at different positions of the sample, for example, in the upper and lower parts of the body cavity, its wounds, etc. due to the flexibility of the combined cable, this reduces the measurement time, because it does not require conversion of power to intensity, as in measurements without light cables.

The disadvantages of the 1st group:

1. Errors due to non-compliance with the correct abutment of the working end.

2. It is not intended to measure the absolute concentration of fluorescents.

3. It is not foreseen to measure almost simultaneously the concentration of all luminescent in the sample.

As the 2nd analogue of the device, the LESA-6 concentrator was selected from the aforementioned series of analogues of laser-luminescent concentrometers, therefore it differs only in more thorough refinement of its parts, the introduction of the necessary computer programs, a wheel table as the basis, etc. [2].

As the 2nd analogue of the method, a laser-fluorescence method for measuring the relative concentration of fluorescents in the hard tissues of the tooth and the layers on it is selected from which the working end face of the tuned laser-luminescent concentmeter is placed in various parts of the tooth from the aforementioned analogues. The smallest value of K _{f is} taken as the reference value, all other values are divided by it, and the quotients of these divisions are the relative concentrations of fluorescents in the remaining parts of the tooth [2]. It is also possible to measure the relative concentration of fluorescents on various teeth, the duration of each measurement in one area of the tooth also does not exceed 1 minute.

The advantage of the 2nd group of analogues: allows you to measure the relative concentration, and not in arbitrary, but fractional, i.e. real units, for example, as a percentage.

The disadvantages of the 2nd group of analogues are the same as that of the 1st group of analogues.

As a prototype of the device, a laser-luminescent concentration meter was selected, which contains a laser, a spectrometer, a U-shaped system of light cables that optically connect them during measurement, a computer with programs for highlighting, storing and printing graphs of the spectral distribution of intensities introduced into the spectrometer, their integrated intensities and mathematical actions on them, sets of training samples, test media and calibration graphs and the basis for placing its parts (see p.2, lines 45 ... 50) [3]. This representative of the aforementioned series [1, 2] was developed to measure the absolute concentration of fluorescent or phosphorescent.

The prototype "Spectrolux MB" differs from its analogues in the following:

1. A flat (face) 4 is made on the carrier in the form of a test tube, motionless abutment of the working end manually by it is possible in a single position (conjugation of the end plane with the face), which is automatically correct, which provides a significant reduction in errors.

2. The prototype is equipped with a set of valid calibration graphs (briefly: “graph”), pre-made on paper for fluorescents and phosphorescent, which provides a measurement of their absolute concentration.

In the prototype, 2 first disadvantages of the device analogs are eliminated, this is an advantage of the prototype.

The disadvantage of the prototype device is the preservation of the third drawback: the lack of a means for measuring all the luminescent in the sample almost simultaneously.

As a prototype of the method, a laser-luminescent method was chosen for a minute measurement of the absolute concentration of luminescent, in which the working end of the combined cable of the tuned laser-luminescent concentration meter rests on face 4 of the carrier with a sample, the name of the luminescence is determined by the shape of the hill on the graph of the spectral intensity distribution on the computer screen the luminescence coefficient and the calibration curve determine the absolute concentration of luminescence [3].

This prototype differs from the detailed description of the 1st analogue of the method as follows:

1. The end face is abutted on face 4 on the carrier in the form of a test tube, therefore, the end face is automatically supported almost correctly, this significantly reduces errors.

2. The value of K _f or K _phos is applied to the abscissa axis of the corresponding previously executed actual graph on paper and the absolute concentration of fluorescents and phosphorescent on the ordinate axis is separately calculated.

The disadvantage of the prototype method: there is virtually no simultaneous measurement of all luminescent in the sample.

The technical result of the present invention of the concentration meter is to eliminate the disadvantage of its prototype: the lack of a means for measuring all luminescent in the sample almost simultaneously.

The specified technical result is achieved by the fact that the laser-luminescent concentration meter containing a laser, spectrometer, a U-shaped system of light cables optically connecting them during measurement, a computer with programs for highlighting, storing and printing graphs of the spectral distribution of intensities in the form of a peak and hills, their integral intensities and mathematical operations on them, sets of training samples, test media and calibration graphs and the basis for placing its parts, according to the offer The invention is equipped with a beam chopper, a program for highlighting hills from luminescent and their boundary hills, a program for calculating difference hills, a program for calculating the integral intensities of luminescent with their tolerances, a program for calculating the excitation coefficients of luminescent, a program for printing a graph of concentration versus time, a set of training samples is supplemented samples for all measured values, each carrier is equipped with a protective cover that prevents excitation forestsentov accidental lighting, with the same reflective properties as the carrier; in addition, a program for creating electronic calibration graphs for luminescents; in addition, a program for automatic measurement of luminescent concentrations according to electronic calibration graphs; in addition, a short combined cable is attached to the combined cable with an optical switch for correct abutment in the delivered carriers and parallel long combined cables with ends that are correctly abutted in the transparent sections of the bioinstallation products in radial holes or by other means; finally, spectrometers are attached to the computer, the working areas of which cover all measured luminescents, optically connected by U-shaped cables to lasers of different frequencies, causing luminescence of all measured luminescent, lasers and spectrometers are fixed on a common basis or on separate bases.

Figure 1 - schematic diagram of a U-shaped system of light cables;

figure 2 - hill from constant phosphorescence;

figure 3 - a hill from luminescent;

figure 4 - the total hill from phosphorescent;

5 is a hill from the excited phosphorescence;

6 is a hill from the fluorescence;

7 - values of the integrated intensities of the light flux;

Fig.8 is a graph of the dependence of the concentration of luminescent on time.

(фиг.5) и Х_ф (фиг.6). «Разностный холм» - обобщающее название холмов, вычисленных как разность ординат соответствующих холмов. Компьютер снабжен программой вычисления интегральных интенсивностей от люминесцентов с их допусками (фиг.7). Компьютер снабжен программой вычисления по ф. (3) коэффициента возбуждения флуоресценции и по подобной формуле - для коэффициента возбуждения фосфоресценции. Компьютер снабжен программой печатания графика зависимостей концентраций люминесцентов от времени (фиг.8). Набор настроечных образцов дополнен образцами для всех измеряемых величин. Их оптические свойства соответствуют измеряемым люминесцентам. Каждый носитель снабжен предохранительным чехлом, предотвращающим возбуждение фосфоресцентов от случайного освещения, с такими же отражательными свойствами, как у носителя.Description of the distinctive part of claim 1 of the claims of the present invention. The claimed Spectrolux BT concentrator (biotechnological) is the next step in the development of the mentioned series of concentrometers after the prototype, because it allows you to measure luminescent almost simultaneously. “Spectrolux BT” is equipped with a beam chopper (“chopper”), which contains a housing with means for embedding between the laser and the laser cable on opposite sides, for example with nuts, a latch with 2 positions: “open” and “closed”. The case is located in a soft, sealed case, which prevents the penetration of dust and vapors into it, which could become pollutants at the ends of the laser and laser fiber. The computer is equipped with a program for highlighting hills from luminescent and their boundary hills (to be explained in the description of the method according to figure 2 ... 6). “Boundary hill” - the maximum permissible deviation of the highlighted hill from its nominal shape according to the technological standard of the enterprise. The computer is equipped with a differential hill calculation program, i.e.

(Fig.5) and X _f (Fig.6). "Difference hill" is a generalized name for the hills, calculated as the difference of the ordinates of the corresponding hills. The computer is equipped with a program for calculating the integrated intensities of the luminescent with their tolerances (Fig.7). The computer is equipped with a calculation program for f. (3) the fluorescence excitation coefficient and, according to a similar formula, for the phosphorescence excitation coefficient. The computer is equipped with a program for printing a graph of the dependences of the concentration of luminescent on time (Fig. 8). The set of training samples is supplemented with samples for all measured values. Their optical properties correspond to the measured luminescents. Each carrier is equipped with a protective cover that prevents the excitation of phosphorescent from accidental lighting, with the same reflective properties as the carrier.

Description of claim 2 of the formula. The computer is equipped with a program for creating electronic calibration graphs for all measured luminescents and storing them with the possibility of displaying, on a manual command, the desired graph, introducing the measured integrated intensity into it and highlighting the absolute concentration of the luminescent.

Description of claim 3 of the formula. The computer is equipped with an electronic program for automatic measurement of the absolute concentration of luminescents by electronic calibration graphs available in the computer.

Description of claim 4 of the claims. If carriers in the form of test tubes are delivered not in a minute, but less often, then the concentrator will stand idle for some time; to eliminate this drawback, a fiber optic splitter (not shown) is attached to the main combined cable 13, to which are connected via fiber optic switches: the same combined cable as the main cable 13 (or shorter) for abutment in the delivered carriers, and parallel long combined cables, they carried out in the workshop prior to bio-installation, each end is fixed in the radial hole of the clamp on the product line with the correct abutment of its working end in a transparent section of the wall of the product line. The end of each combined cable can be fixed by other means.

Description of claim 5 of the formula. The larger the number of luminescent measured, the greater the ability to produce a quality product. For this, several lasers of various frequencies with choppers are fixed on the basis, one or several spectrometers (they can be on other bases) connected to the bioinstallation pipelines by a network of laser, combined and spectrometer cables based on U-shaped cables through optical switches and splitters. Lasers cause luminescence of all phosphors in the product, spectrometers decompose all luminescent streams into spectra, a computer prints the peaks and hills of all lasers and luminescents, ensuring the implementation of the method. Such a network makes it possible to measure the concentration of a larger number of luminescents in different frequency regions so that the hills do not intersect, which is especially useful for related luminescents: constant phosphorescent, excited phosphorescent or fluorescents.

The technical result of the invention of the method is to eliminate the disadvantage of the prototype, i.e. the absence of almost simultaneous measurement of all luminescent in the sample.

The indicated technical result is achieved by the fact that in the laser-luminescent method for minute measurement of the absolute concentration of luminescent, in which the working end of the combined cable of the tuned laser-luminescent concentmeter is abutted against the face of the carrier with a sample, the name of the luminescence is determined on the computer screen on the graph of the spectral intensity distribution on the computer screen measure the absolute concentration of luminescence by the luminescence coefficient and the calibration graph, according to the proposed the beam is interrupted, the end face is pressed against the carrier, the boundary hills highlighting program is turned on and a constant phosphorescence hill is obtained, its integrated intensity, boundary hills are measured, the constant phosphorescence concentration is measured by its integrated intensity and the actual calibration graph, the sample is illuminated with a beam and a peak and hill are obtained from luminescents, interrupt the beam and get a hill from phosphorescent, turn on a differential hill calculation program and get a hill from excited phosphorescent as well as the fluorescence and their boundary hills, include a program for calculating the integrated intensities of luminescence with their tolerances and get their values, include a program for calculating the excitation coefficients of luminescence and get their values, measure their concentration according to the excitation coefficients of luminescence and the actual calibration graphs, include a program for printing the dependency graph concentrations over time, monitor the progress of the process and make decisions to eliminate its deviations, in addition, ysvechivayut on manual command Fluorescent calibration curve measured is introduced into it measured the integrated intensity or luminescence factor, the computer displays the ordinate point corresponding to the measured concentration value and its concentration dependency graph of time; further include a program for automatically measuring the absolute concentration of luminescent; then, in between measurements of samples in the delivered carriers, the concentration of luminescent in the product lines of the biostation is measured, for which the fibers of the first long combined cable are connected to the fibers of the combined cable and the steps are performed according to claim 6, then switch the switch to the next long cable and repeat these actions; further, interrupting the beams of different lasers and switching lasers and spectrometers, measure the concentration of more luminescents; finally, calibration graphs are performed at the manufacturing plant with respect to operating conditions in the field of ambient temperature, which does not cause unacceptable errors during calibration and measurements, and for measurements at a different temperature, the concentrator is re-calibrated at this temperature.

Description of the characterizing part of the 6th claim. Measurements in biotechnology have features, so their preparation also has features. Spectrolux BT is installed in the workshop in a laboratory room equipped according to the enterprise standard, in the wall there is a lockable hole with a movable tray with a spring clip for the delivered carrier in the form of a test tube, for example, with a liquid sample of the product; local fixtures are provided; all surfaces, including Spectrolux BT, are made with the greatest possible absorption of light from fixtures to prevent the excitation of luminescent in the sample. During preparation for the measurement, a sample of the product is placed at regular intervals in a carrier, for example, a tube that is clean inside and out, in a sheath that does not allow streams that can excite phosphorescence in the sample and that have the same reflective properties as the tube. The tube is delivered to the closable hole in the wall of the laboratory room no later than the time when changes in the concentration of luminescent exceeding the measurement errors can occur, and transmit to the measuring one through the tray along with the form of the measurement results. The measuring one rests the working end face in the tube cover and checks if there is no hill due to exposure, if there is a hill, the cause of exposure is eliminated. The test tube cover is placed on the tray, the tube is cleaned again. The preparation is over. We introduce a condition that simplifies understanding: all hills are flat, of different heights, therefore, do not intersect.

Measurement sequence:

от постоянного фосфоресцента, может быть, хемифосфоресцента, черной линией и граничные холмы допуска на этот холм более тонкими линиями того же цвета (на фиг.2 - непрерывными линиями) и интегральную интенсивность

Измеряющий приблизительно оценивает концентрацию этого фосфоресцента по положению

между граничными холмами. График повторяется на компьютере технолога, а если

хотя бы коснется одного из граничных холмов, измеряющий докладывает об этом технологу, и тот принимает меры по устранению этого нарушения. В последующих пунктах называются основные измерительные действия, а их логичные следствия не упоминаются, но выполняются.1. The meter interrupts the beam, for which it pushes the interrupter latch, the end face correctly rests against the flange 4 on the test tube and includes a program for highlighting the boundary hills. The computer programs from the prototype and the aforementioned program calculates, displays, remembers and prints a hill as a document

from a constant phosphorescence, maybe a chemiphorescence, the black line and the boundary hills of admission to this hill with thinner lines of the same color (in Fig. 2 - continuous lines) and the integrated intensity

The meter approximately estimates the concentration of this phosphorescent by position

between the boundary hills. The schedule is repeated on the technologist’s computer, and if

at least it touches one of the boundary hills, the measuring officer reports this to the technologist, and he takes measures to eliminate this violation. In the following paragraphs, the main measuring actions are called, and their logical consequences are not mentioned, but are performed.

2. The measuring person or his assistant measures the concentration of the constant phosphorescent by its integral intensity and the actual graph, for which the abscissa of the graph marks the value of the integral intensity, restores the perpendicular to the intersection with the calibration line, drops the perpendicular from the intersection point to the ordinate and counts the concentration.

измеряющий освещает пучком пробу, для чего выдвигает задвижку, компьютер печатает пик П красной линией (на фиг.3 - в виде треугольника) и люминесцентный Х_л по формуле

(от фосфоресцента, возбужденного пучком) + Х_ф (от флуоресцента) голубой линией с граничными холмами (на фиг.3 штрих-пунктирной линией, далее граничные холмы не упоминаются, но выполняются).3. After several measures, ie after flashing

the measuring one illuminates the sample with a beam, for which it extends the valve, the computer prints peak P with a red line (in figure 3 - in the form of a triangle) and luminescent X _l according to the formula

(from the phosphorescent excited by the beam) + X _f (from the fluorescence) with a blue line with boundary hills (in Fig. 3, a dash-dotted line; further, boundary hills are not mentioned, but are executed).

коричневым цветом (фиг.4 - линией «-+-+»).4. After several cycles, the meter interrupts the beam, for which it pushes the chopper shutter. Computer prints total phosphorescent hill

brown color (figure 4 - line "- + - +").

и печатает

фиолетовой линией (на фиг.5 - прерывистой линией), и сразу же компьютер вычисляет и печатает флуоресцентный холм

желтым цветом (на фиг.6 - точечной линией).5. The meter includes a differential hill calculation program, and the computer calculates

and prints

a violet line (in FIG. 5 a dashed line), and immediately the computer calculates and prints a fluorescent hill

yellow (in Fig.6 is a dotted line).

6. The meter immediately starts the program for calculating the integrated intensities, and the computer calculates by f. (2), and prints the values of the integrated intensities of the luminescent, i.e. excited phosphorescence and fluorescence in the form of vertical lines depicting their tolerance fields with a cross at the height of its value and the numerical value assigned to them by the colors (in Fig. 7 they are indicated by letters with the indices of the corresponding hills) and the integral intensity of peak P by f. (one).

7. The meter includes a program for calculating the excitation coefficients of the luminescent, and the computer calculates K _f on f. (3) and K _phos according to the same formula with the replacement of the index "f" by "phos" and prints their values.

и действительным градграфикам для флуоресцента и возбужденного фосфоресцента, для чего из значения К_ф

на оси абсцисс восстанавливают перпендикуляр до пересечения с градуировочной линией, из точки пересечения опускают перпендикуляр на ось ординат и отсчитывают концентрацию.8. The meter measures the concentration of the aforementioned luminescents by K _f and

and the actual graphs for fluorescence and excited phosphorescence, for which from the value of K _f

on the abscissa, the perpendicular is restored to the intersection with the calibration line, from the intersection point the perpendicular is lowered to the ordinate and the concentration is counted.

был принят постоянным, ибо пучок во время первого такта возбудил все центры фосфоресценции, а во втором такте они остались возбужденными и не успели снизить мощность более погрешности измерения. Без упрощающего условия холмы будут пересекаться, это не вызовет трудностей для компьютера, но потребует больше времени для понимания способа, поэтому было введено это упрощение. Трудности начнутся, когда будут однородные люминесценты, например 2 флуоресцента, холмы которых пересекутся, однако компьютер по несовпадающим частям этих холмов сможет вычислить холмы полностью, ибо они известны для любой концентрации. Если же их участки на оси абсцисс спектрографика совпадают, то можно сделать их не совпадающими, освещая пробу лазерами разного цвета (в заявке «цвет» следует понимать как краткое название соответствующего участка спектра от УФ до ИК) одновременно или в промежутках между измерениями другим лазером. Тогда люминесцент может изменить цвет люминесценции (холм будет на другом участке спектра), изменить род люминесценции, пусть на том же участке, холм может быть высвечен в другое время, может быть использован запасный люминесцент, это даст возможность использовать этот способ видоизмененным по п.10 формулы изобретения. Использование УФ-лазера создаст неудобства для измеряющего из-за того, что он не будет видеть пучок, и потребует ужесточение требований охраны труда, что не препятствует использованию способа.9. The meter includes a program for printing a graph of the dependences of concentrations on time, enters the concentrations in them, and keeps a graph "luminescent concentration = f (t)" with tolerance lines for concentration (Fig. 8). The measurement is completed, the computer took all the measurements, so the measurement took less than a minute.

was taken constant, because the beam excited all phosphorescence centers during the first cycle, and in the second cycle they remained excited and did not have time to reduce the power more than the measurement error. Without simplifying conditions, the hills will intersect, this will not cause difficulties for the computer, but will require more time to understand the method, therefore this simplification was introduced. Difficulties will begin when there will be homogeneous luminescents, for example 2 fluorescents, the hills of which intersect, but the computer can completely calculate the hills from the mismatched parts of these hills, because they are known for any concentration. If their sections on the abscissa axis of the spectrographics coincide, then they can be made different by illuminating the sample with lasers of different colors (in the application “color” should be understood as the short name of the corresponding part of the spectrum from UV to IR) simultaneously or in the intervals between measurements by another laser. Then the luminescence can change the color of the luminescence (the hill will be in a different part of the spectrum), change the kind of luminescence, even in the same area, the hill can be highlighted at another time, a spare luminescence can be used, this will make it possible to use this method modified in accordance with claim 10 claims The use of a UV laser will create inconvenience for the measuring person due to the fact that he will not see the beam and will require stricter labor protection requirements, which does not preclude the use of the method.

и К_ф измеряющий вводит их в электронный градграфик, и компьютер высвечивает числовое значение концентраций возбужденного фосфоресцента и флуоресцента. Числовые значения люминесцентов измеряющий вводит в программу печатания графиков зависимостей концентраций люминесцентов от времени (фиг.8).Description of claim 7 of the formula. To increase labor productivity during measurements, a program was developed to create electronic graphs for luminescents with the option of displaying the desired graph on a manual command, introducing the measured integrated intensity into it and highlighting the absolute concentration of luminescence. The meter previously created electronic gradographs for each luminescent, for which it included this program, entered the data of the actual graphs in it, i.e. created electronic gradographs. In the measurements, after measuring the integral intensity of the constant phosphorescent, it measures its numerical value on the keyboard, the computer displays the corresponding point on the abscissa axis, the perpendicular to the intersection with the calibration line, the corresponding point on the ordinate axis and the numerical concentration value. After measurement

and the K _f measuring device enters them into the electronic graph, and the computer displays the numerical value of the concentrations of excited phosphorescence and fluorescence. The numerical values of the luminescent measuring device enters into the program for printing graphs of the dependences of the concentration of luminescent on time (Fig. 8).

Description of claim 8 of the formula. Based on the program for creating electronic graphs (see description of claim 7 of the formula), a program was developed to automatically measure the concentration of luminescents from electronic graphs. After obtaining the numerical values of the integrated intensities of constant phosphorescence and fluorescence, the measuring device enters their numerical values into the program for automatic concentration measurement, the computer displays their concentrations and transfers the graph of the dependences of the concentrations on time to the printing program (Fig. 8).

Description of paragraph 9 of the formula. It is designed to measure the product in the biostation product pipeline, i.e. in relation to claim 4 of the claims. Between measurements of samples in delivered carriers, the concentration of luminescent in the product lines of a biostation is measured, for which an optical switch (not shown) is connected to the fibers 11a and 12a of the main combined cable 13 with the same fibers of the first same but long combined cable (not shown), end which rests on the transparent part of the wall of the product pipeline, and perform the steps according to claims 6 to 8 of the claims. After measurement, switch these fibers to another same long cable and repeat the measurements.

Х_л будут высвечиваться из разных участков движущегося продукта, однако можно принять условие, что за время между 1-ми тактами высвечивания (доли секунды) участок освещенного пучком объема продукта не успеет уйти из объема, откуда попадают потоки от возбужденных люминесцентов, поэтому описанные действия измеряющего и результаты измерения будут правильными, причем измерения можно выполнять проще и чаще, чем при доставке пробирок к концентратомеру. Измеряющий не только измеряет, но и следит за приближениями холмов к граничным холмам и при угрозе касания докладывает технологу, у которого есть такой же компьютер, подключенный параллельно, технолог принимает меры для предотвращения отклонений холмов от заданных технологической документации. При слишком большой скорости продукта возникает задача изобретения нового способа.Given the features of this measurement: product movement during measurement, attention should be paid to the possibility of error, because

X _l are displayed from different portions of a moving product, but can adopt a condition that the time between the 1st and strokes luminescence (a split second) portion illuminated by a beam volume of the product does not have time to escape from the volume from which flows from the excited luminescent fall, so the actions described measuring and the measurement results will be correct, and the measurements can be performed easier and more often than when delivering the tubes to the concentrator. The meter not only measures, but also monitors the approach of the hills to the boundary hills and, if there is a threat of contact, reports to the technologist who has the same computer connected in parallel, the technologist takes measures to prevent deviations of the hills from the given technological documentation. If the product speed is too high, the task of inventing a new method arises.

Description of paragraph 10. If a sample needs to measure luminescents that are not excited by a single laser, or luminesce outside the working area of the spectrometer, or the hills coincide, causing errors, etc., then use the right number of lasers and spectrometers connected by the right amount of U-shaped light cables with the required number of computers perform the steps of the previous claims. This is a more complex method, it requires preliminary studies to choose lasers of such colors that excite luminescence, which do not coincide with others. The actions of the measuring will differ in that he will work with different lasers in a given sequence, and the computer will work on computer programs.

Description of paragraph 2.5. The beam power varies depending on the laser temperature, the intensity of the flows behind the filter and devices for this purpose varies with their temperature, the power of the signals of the photoelectric converters varies with temperature, therefore, to reduce the temperature errors, the graphs are performed by the manufacturer in relation to operating conditions at a given temperature, not causing unacceptable errors during graduation and measurements. According to medical requirements, the temperature error should be within ± 5% for normal conditions. This is feasible when the concentrator is operated in clinics under normal conditions, if graduation was performed at other temperatures, for example, in workshops at a different ambient temperature.

The advantage of the group is that it continuously monitors the deviations of all concentrations of the given luminescence, which allows to simultaneously increase the quality and quantity of the product while reducing the cost of its production, including reimbursement of defective products, which has decreased.

Information sources

1. Alexandrov M.T. and other diagnostic device. Application to the Patent Office of the United Kingdom No. GB 96/02609, 10.24.96

2. Alexandrov M.T. and others. A method for the diagnosis of hard tissues of the tooth and its deposits. Pat. RF №2112426, MKI А61В 6/00, 1997/98

3. Alexandrov M.T. et al. A test carrier and a method for quickly measuring the absolute concentration of bacteria in a biosubstrate by their luminescence (options). Pat. RF №2255978, MKI C12Q 1/06, 2002/2004 (prototype).

Claims (11)

1. Laser-luminescent concentrator containing a laser, spectrometer, U-shaped system of light cables with the main combined light cable, optically connecting them during measurement, a computer with programs for highlighting, storing and printing graphs of the spectral distribution of intensities in the form of a peak and hills, their integral intensities and mathematical operations on them, sets of training samples, test media, calibration graphs and the basis for placing its parts, characterized in that it is equipped with a beam generator, which contains a housing with means for embedding it between the laser and the laser light cable, while each sample carrier is equipped with a protective cover that prevents phosphorescent excitation from accidental illumination, with the same reflective properties as the medium, the computer uses a program for highlighting boundary hills, programs for calculating difference hills, programs for calculating and storing the values of the integral intensities of luminescence from luminescents and their tolerances s, calculation program, storing and printing Fluorescent excitation coefficients and dependencies graphics printing program concentrations against time. 2. The concentration meter according to claim 1, characterized in that it is equipped with a program for creating electronic calibration graphs for all measured luminescents with the ability to display the desired graph on a manual command and display the absolute concentration of the luminescent. 3. The concentration meter according to claim 1, characterized in that it is equipped with a program for automatically measuring the absolute concentration of luminescents according to electronic calibration graphs. 4. The concentrator according to claim 1, characterized in that a fiber optic splitter is connected to the main combined light cable, to which the same combined cable is connected via fiber optic switches or shorter for abutment in delivered carriers and parallel long combined cables, each end of which is fixed in a radial hole the clamp on the product pipeline when abutting the end face in a transparent section of the wall of the food product. 5. The concentration meter according to claim 1, or 2, 3, or 4, characterized in that additional spectrometers are attached to the computer, the working areas of which cover all the measured luminescents, optically connected by U-shaped light cables to additional lasers of different frequencies, causing luminescence of all measured luminescents, while lasers and spectrometers are fixed on a common basis or on separate bases. 6. The laser-luminescent method for minute measurement of the absolute concentration of luminescent, in which the working end of the combined light cable of the tuned laser-luminescent concentration meter rests on the face of the carrier with the sample, the name of the luminescence is determined from the shape of the hill on the graph of the spectral intensity distribution on the computer screen and the value of the luminescence coefficient on the calibration graph determine the absolute concentration of the luminescent, characterized in that the beam interrupter interrupt azero beam, abut the working end face in the carrier with a protective cover, turn on the program for highlighting boundary hills and get a hill from constant phosphorescence, its integral intensity and boundary hills, measure the concentration of constant phosphorescence from its integrated intensity and the actual calibration graph, illuminate the sample with a laser beam and get peak and hill from luminescents, interrupt the beam and receive a hill from phosphorescents, include a differential hill calculation program and receive a hill from of excited phosphorescence and fluorescence and their boundary hills, include a program for calculating the integrated intensities of luminescents with their tolerances and obtain their values, include a program for calculating the luminescence excitation coefficients and obtain their values, measure concentrations from luminescence excitation coefficients and actual calibration graphs, and include a dependency graph printing program concentrations from time to time. 7. The method according to claim 6, characterized in that the calibration graph of the measured luminescence is displayed according to a manual command, the measured luminescent flux is introduced into it, the computer displays a point on the ordinate axis corresponding to the measured concentration, its value and prints this value. 8. The method according to claim 6, characterized in that it includes a program for automatically measuring the absolute concentration of luminescent. 9. The method according to claim 6, characterized in that they use a combined light cable and long combined light cables, while additionally measuring the concentration of luminescent in the product pipelines of biostations, for which an optical switch connects the same main fibers to the fibers of the main combined cable
fibers of the first same, but long combined cable, the end of which is abutted in the transparent part of the product pipeline wall, and after measurement, switch these fibers to another same long cable and repeat the measurements. 10. The method according to claim 6, characterized in that they use different lasers, while interrupting the beams of different lasers and switching lasers and a spectrometer, measure the concentration of a larger number of luminescents. 11. The method according to any one of paragraphs.6-10, characterized in that the calibration graphs are performed at the manufacturer in relation to operating conditions in the field of ambient temperature, which does not cause unacceptable errors during calibration and measurements, and for measurements at a different temperature, calibration graphs are performed at this temperature.

Images