RU2309617C1 - Phospholipid biologically active food additive having antioxidant properties - Google Patents

Abstract

FIELD: food processing industry, in particular production of biologically active food additives.

SUBSTANCE: phospholipid biologically active food additive having antioxidant properties represents phospholipid product. Said product is obtained by hydration of non-refined high oleic sunflower oil with aqueous solution of monosubstituted sodium citrate with concentration of 1.0-4.0 % pretreated in alternating magnetic field with flux density of 0.1-0.4 Tl at 30-50°C. Further phospholipid emulsion is separated from hydrated oil, and dried to produce target product. Phospholipid biologically active food additive has antioxidant properties due to maximal conservation of native physiologically functional ingredients such as phospholipids including phosphatidyl cholines, phosphatidyl ethanolamines, phosphatidyl serines, and phosphatidyl inozitoles, as well as tocopheroles and beta-carotene.

EFFECT: biologically active food additive of improved quality.

3 tbl, 3 ex

Description

The invention relates to the food industry and can be used to obtain high-quality biologically active additives (BAA), used for direct consumption as a prophylaxis or to create functional food products.

Known food phospholipids obtained from vegetable oil (Technology of processing fats / N.S. Harutyunyan, E.P. Kornen, L.I. Yanova and others. Under the editorship of Professor N.S. Harutyunyan. - 3rd ed. - M.: Pishchepromizdat, 1999, - 452 p.).

However, such phospholipids, due to the conditions for their preparation, do not have antioxidant properties, because the presence of harmful impurities in them - lysoform of phospholipids, peroxides, and secondary oxidation products does not make it possible to recommend these phospholipids as dietary supplements for direct consumption and for the creation of functional food products.

The objective of the invention is the creation of a phospholipid biologically active food supplement with antioxidant properties.

The problem is solved in that a phospholipid biologically active food supplement having antioxidant properties is characterized in that it is a phospholipid product obtained by hydrating unrefined high oleic sunflower oil with an aqueous solution of monosubstituted sodium citrate in a concentration of 1.0-4.0%, pretreated in an alternating electromagnetic field with a magnetic induction of 0.1-0.4 T at a temperature of 30-50 ° C, separating the resulting phospholipid emulsion from hydrated oil followed by drying the phospholipid emulsions to give the desired product.

The technical result is to give the phospholipid biologically active additive antioxidant properties, which is achieved through the use of mild production modes that maximize the native preservation of physiologically functional food ingredients such as phosphatidylcholines, phosphatidylserines, phosphatidylinositols, phosphatidylethanolamines and tocopherols.

As we have shown experimentally, upon receipt of the inventive phospholipid dietary supplement according to the indicated modes, the necessary physiologically functional ingredients that provide an antioxidant effect are stored in it in their native form.

Data characterizing the antioxidant effect of the claimed phospholipid dietary supplement, confirmed in experiments on white rats, 25% of the diet of which was provided by the claimed phospholipid dietary supplement.

The control group of animals did not receive the claimed phospholipid dietary supplement.

Feeding was carried out according to the principle of "plenty" with free access to water. The duration of the experiments was 3 months.

As indicators characterizing the effect of the claimed phospholipid dietary supplement on the processes of peroxidation, the content of malondialdehyde (MDA) in the blood serum and the activity of superoxide dismutase (SOD) were determined.

The antioxidant effect of the claimed phospholipid dietary supplement is illustrated by examples, the initial indicators of which were the modes of its production, and experimentally determined indicators are the content of malondialdehyde (MDA), which serves as an indicator of the state of lipid peroxidation in the body, as well as superoxide dismutase (SOD) activity, which is one from important indicators of the activity of the enzymatic antioxidant defense of the body.

Example 1. A phospholipid biologically active food supplement is a phospholipid product obtained by hydrating unrefined high oleic sunflower oil with an aqueous solution of monosubstituted sodium citrate at a concentration of 1.0%, pre-treated in an alternating electromagnetic field with magnetic induction of 0.4 T at a temperature of 30 ° C separating the resulting phospholipid emulsion from the hydrated oil and then drying the phospholipid emulsion to obtain the desired product.

The values of the studied indicators are shown in table 1.

Table 1 Indicators The experimental group (the claimed phospholipid dietary supplement) Control group The content of MDA in serum, nmol / ml 4.1 9.8 SOD activity, relative units / mg protein 6.6 3.6

Example 2. A phospholipid biologically active food supplement is a phospholipid product obtained by hydration of unrefined high oleic sunflower oil with an aqueous solution of monosubstituted sodium citrate concentration of 2.5%, pre-treated in an alternating electromagnetic field with magnetic induction of 0.3 T at a temperature of 40 ° C separating the resulting phospholipid emulsion from the hydrated oil and then drying the phospholipid emulsion to obtain the desired product.

The values of the studied indicators are shown in table 2.

table 2 Indicators The experimental group (the claimed phospholipid dietary supplement) Control group The content of MDA in serum, nmol / ml 4.0 9.8 SOD activity, relative units / mg protein 6.5 3.6

Example 3. A phospholipid biologically active food supplement is a phospholipid product obtained by hydration of unrefined high oleic sunflower oil with an aqueous solution of monosubstituted sodium citrate at a concentration of 4.0%, pretreated in an alternating electromagnetic field with magnetic induction of 0.2 T at a temperature of 50 ° C separating the resulting phospholipid emulsion from the hydrated oil and then drying the phospholipid emulsion to obtain the desired product.

The values of the studied indicators are shown in table 3.

Table 3 Indicators The experimental group (the claimed phospholipid dietary supplement) Control group The content of MDA in serum, nmol / ml 3.9 9.8 SOD activity, relative units / mg protein 6.7 3.6

From the above data it is seen that the claimed phospholipid biologically active food supplement is a natural physiologically valuable plant product with antioxidant properties, and can be recommended for direct use as an independent prophylactic or against the background of general diet therapy, as well as to create functional food products.

Claims (1)

A phospholipid biologically active food supplement with antioxidant properties, characterized in that it is a phospholipid product obtained by hydrating unrefined high oleic sunflower oil with an aqueous solution of monosubstituted sodium citrate with a concentration of 1.0-4.0%, pre-treated in an alternating electromagnetic field with magnetic induction of 0.1-0.4 T at a temperature of 30-50 ° C, separation of the resulting phospholipid emulsion from hydrated oil and subsequent drying folipidnoy emulsion to give the desired product.