RU2300812C2 - Method for modeling epiphyseal aseptic necrosis under experimental conditions - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: method involves creating blood circulation disorder conditions in soft tissues of tubular bone epiphysis. To do it, needle with mandrin are introduced into epiphysis under study under general anesthesia condition in aseptic mode. 1 ml of mixture is introduced via the needle after removing mandrin from the needle. The mixture contains equal parts of 10% calcium chloride solution and 96% ethyl alcohol.

EFFECT: improved aseptic necrosis model reproducibility as a result of additional mechanical injury of epiphysis tissue.

Description

The invention relates to medicine, namely to traumatology and orthopedics, and is intended for scientific research in the treatment of epiphyseal aseptic necrosis.

A known method of modeling epiphyseal aseptic necrosis (EAN) in an experiment by electrolysis, in which, under the influence of an electric field, it was possible to cause circulatory disturbance in the bone tissue of the pineal gland pineal gland [2, 3].

A known method of simulating EAN by injection into the arterial vessels of a suspension of carcasses [4].

The disadvantages of the described analogues of the claimed invention is the low efficiency of the method.

A known method of modeling EAN in the study of the etiopathogenesis of osteochondropathy in an experiment, which consists in circular excision of the capsule of the hip joint, muscle ligation and the destruction of the endosteal metadiaphysis of the femur [1]. The disadvantage of this method is the complexity and complexity of providing EAN in the experiment.

There is a method of modeling aseptic epiphyseal necrosis described in the patent of the Russian Federation for the invention "Method for the treatment of epiphyseal aseptic necrosis" No. 2182473, Cl. A61B 17/56, 05.20.2002, p. 4, 6 paragraph, which consists in introducing into the pineal gland 1 ml of a solution consisting of equal parts of 10% calcium chloride and 96% ethyl alcohol.

The disadvantage of this method is the short duration and insufficient severity of achieved aseptic necrosis. For the purpose of this method, for some effects on bone tissue, as well as the number of essential features common with the claimed invention, this method is selected as a prototype.

An object of the invention is to achieve a stable long-term aseptic epiphyseal necrosis in the experiment.

The essence of the invention lies in the fact that in the method of modeling epiphyseal aseptic necrosis in an experiment based on creating conditions of circulatory disorders in the bone tissue of the pineal gland of the tubular bone by introducing into the test pineal gland 1 ml of a mixture consisting of equal parts of 10% calcium chloride and 96% of ethanol, a needle with a mandrel is introduced into the pineal gland under general anesthesia under aseptic conditions, after the mandrene is removed from it, the mixture is injected through the needle under pressure.

The introduction of the solution is carried out with additional destruction of the bone tissue of the pineal gland due to mechanical injury of the tissue of the pineal gland by introducing a needle with a mandrin into the pineal gland, followed by injection of the mixture into the pineal gland under pressure through this needle after extraction of the mandrin from it, which creates additional barotrauma of the bone tissue and leads to to persistent prolonged aseptic necrosis of the pineal gland.

The achievement of the technical result of the invention provides significant distinguishing features of the method, expressed by a combination of actions, namely: the introduction of the needle with the mandrin into the pineal gland, the introduction of the mixture into the pineal gland under pressure through the needle after removing the mandrin from it.

The drawing shows a diagram of a method for modeling aseptic epiphyseal necrosis.

Description of the method.

Under general anesthesia under aseptic conditions, a needle 2 with a diameter of two millimeters with mandrin at a right angle is inserted into the studied pineal gland 1. The mandrin is removed, a syringe 3 with a mixture of chemicals is connected to the needle and 1 ml of a mixture of 10% calcium chloride with 96% ethyl alcohol in a 1: 1 ratio is injected into the pineal gland under pressure, the needle is removed and an aseptic dressing is applied.

The experiment was conducted on 30 outbred puppies of both sexes at the age of 1.5 months, with an average weight of 1.5 kg. The experiments were carried out on the right limbs, the left was used for control studies.

All studies and sampling of experimental material were carried out after 1 week, 2 weeks, 1 month, 2 months, 3 months after the experiment.

At the indicated times after the experiment, the intraosseous pressure was measured using the Waldman apparatus, followed by histological examination of the bone tissue in the experimental and control pineal glands.

Results: intraosseous pressure after a week did not differ from the control and corresponded to 0.75 kPa. Histologically, during this period, edema of the connective tissue of the bone marrow cavities, plethora of blood vessels, and subchondral necrosis were determined. After 2 weeks, the pressure increased to 0.9 kPa (1.2 times more than the control). In bone tissue - pronounced paresis of blood vessels, edema and leukocyte infiltration, autolysis.

One month after the experiment, intraosseous pressure increased to 2.0 kPa (2.7 times more than the control). In bone marrow cavities, there was an increase in edema and lymphocytic infiltration, pronounced bone resorption. After 2 months, there was a maximum increase in intraosseous pressure to 2.8 kPa (3.7 times more than the control). After 3 months, intraosseous pressure was 2.25 kPa (3 times more than the control). In these terms, the formation of bone tissue with an erratic arrangement of bone beams began.

Thus, using this method, a persistent long-term epiphyseal aseptic necrosis was created in the experiment.

Information sources

1. Abakarov A.A., Bogosyan A.B., Melgunov A.V. Stimulation of reparative osteogenesis with aseptic necrosis of the femoral head in the experiment. // Orthopedics, traumatology and prosthetics. - 1986, No. 11 - P.14.

2. Axhaüsen G. Der anatomische Krarikheitsablauf bei der Köhlerschen Krankheit der Metatarsalköpchen und der Perteschen Krankheit des Hüftkopfes. // Arch. klin. Chir. 1923 - S.511-542.

3. Axhaüsen G., Bergman. Ernahrungsunterbrechungen am Knochen. - In: Handbuch der speciellen. Anat. Und histol. Lubarsch. Henke u. Rosle. - Berlin: Knochen und Gelenke. - 1937, Vol. 3.

4. Bukhny A.F. Damage to the pineal areas of bones in children. M .: Medicine, 1973. - 166 p.

Claims (1)

A method of modeling epiphyseal aseptic necrosis in an experiment based on creating conditions of circulatory disturbance in the bone tissue of the pineal gland of the tubular bone by introducing into the studied pineal gland 1 ml of a mixture consisting of equal parts of 10% calcium chloride and 96% ethyl alcohol, characterized in that a needle with a mandrel is introduced into the pineal gland under general anesthesia under aseptic conditions, after the mandrene is removed from it, the mixture is introduced through the needle.