RU2290943C2 - Composition with restorative action - Google Patents

Abstract

FIELD: pharmaceutical industry, in particular restorative agent.

SUBSTANCE: claimed composition contains extracts from grape stalk, green tea, magnolia-vine fruit, liquorice root, and blackberry in specific ratio.

EFFECT: composition with effective restorative action, accelerating restoration after physical exertion, improving psychomotor ability and action of cardiovascular system.

3 cl, 5 tbl

Description

The invention relates to the creation of herbal products with a tonic effect, which in the future can be used to improve the functional state of the body.

Compositions containing caffeine and taurine are known as "energy drinks". Their composition is largely similar: water, vitamins, flavors and caffeine.

There are a large number of studies in the scientific literature on the effects of caffeine. It has been shown that caffeine can compensate for the deterioration of cognitive functions and psychomotor reactions caused by substances that depress the central nervous system, including alcohol [Smith A., Brice C. et al., 2003; Marczinski C.A., Fillmore M.T., 2003; Drake C. L., Roehrs T. et al, 2003; Mackay M., Tiplady B., Scholey A.B., 2002], although some studies do not confirm the antagonistic effect of caffeine on the inhibitory effect of alcohol [Fillmore M.T., Roach E.L., Rice J.T., 2002]. Low doses of caffeine (150 mg) prevent drowsiness and cognitive impairment associated with taking moderate doses of alcohol (0.5 g / kg), that is, caffeine removes the sedative effects of alcohol, although some negative changes persist with the combined use of alcohol with caffeine (for example, dizziness) [Drake CL, Roehrs T. et al., 2003]. In animal (mouse) models, caffeine has been shown to reduce the hypnotic effect of alcohol by blocking type 2A adenosine receptors [El Yacoui M., Ledent C. et al., 2003].

The problem of fatigue occupies a large place in research conducted in the framework of a wide variety of sciences. First of all, this phenomenon is studied in ergonomics, but also in the psychology of labor, sports, engineering, social, pedagogical and space psychology, psychophysiology, human physiology. Studies of this problem are mainly aimed at creating optimal living and working conditions that ensure maximum efficiency and reliability of human work. These tasks are especially important for the activities of the human operator, whose work is constantly accompanied by a state of fatigue. One of the most characteristic types of operator activity is the activity of the driver of a vehicle (car), and in this study, this particular type of activity was used as an imitation of the labor of a human operator.

There are a number of external factors that contribute to the development of a state of fatigue and are harmful to the environment: noise, vibration, infrasound, electromagnetic fields from devices, insufficient or incorrect lighting, monotony of activity, toxic substances, etc. Almost all of these factors affect the driver while driving. The environmental factors are added the need for increased concentration of attention and visual tension, increased responsibility, limited time for decision-making, a long stay in an unchanged position. All these factors are stressful and, ultimately, lead to the development of fatigue.

It is known that the state of fatigue can manifest itself as a decrease in the level of activation in the body, the development of drowsiness, boredom, lethargy (which is especially typical for monotonous activity), and an increase in the level of activation of the body up to overexcitation, irritation, and affective reactions (for example, with increased mental stress ) Therefore, when studying the phenomenon of fatigue and possible methods for its prevention and correction, a special place is occupied by the question of achieving the optimal functional state of a person, in which the efficiency of his activity will be maximum. The optimal state of the body does not arise with minimal or maximum activation, but with an average level of activity of all systems.

An important means of preventing fatigue is the creation of optimal working and resting conditions, the development of voluntary self-regulation skills, the optimal distribution of functions between a person and a machine, giving tools, controls and mechanisms optimal characteristics, etc. In addition to such methods of maintaining working capacity at a constant level, various medication and non-medication stimulants are used for preventive purposes (especially in sports).

For a quick and short-term increase in working capacity in some cases (as prescribed by a doctor), drugs from the group of psychostimulants are used. However, these substances have a wide range of side effects, restrictions on administration, contraindications, negative consequences of administration, in some cases even the possibility of developing drug addiction. Therefore, as a widely used means to increase mental and physical performance, it is advisable to use more mildly active substances of plant and animal origin.

These substances are adaptogenic and tonic, which increase the body's defenses, overall stability and vitality, physical and mental performance, reduce the negative effects of the environment and stress. In contrast to psychostimulants, which lead to the use of the body’s reserves and can ultimately lead to its depletion and paradoxical reactions, adaptogens and actoprotectors contribute to the rational use of the body’s resources, maintaining its functioning for a long time at the optimal level.

Such agents include compositions containing eleutherococcus, lemongrass, aralia, ginseng, etc. (for example, ed. certificate of the USSR No. 583792, 1977, No. 1722397, 1992, Russian patent No. 2011616, 1994).

However, these compositions have limitations and are not indicated for persons with high excitability, suffering from hypertension, because they are all stimulants of the central nervous system. In this aspect, polyphenolic compounds (glycosides, lignans, flavonoids, etc.) can be of significant interest, which, without energy value or a specific action, increase the body's resistance to the action of a large number of extreme factors and their combinations, optimizing adaptation to these factors. Representatives may be compounds contained in known plants, in particular in Chinese magnolia vine, cultivated grapes, tea, and many others (Meerson F.Z., Pshennikova M.G., 1988; Lupandin A.V., 1991; Pilat T.L. ., Ivanov A.A., 2002).

Among the possible mechanisms of action of polyphenolic adaptogens, three main ones can be distinguished. First, the stimulating effect on the central nervous system is associated with the activation of catecholaminergic synaptic transmission, due to the inhibition of catechol-ortho-methyltransferase, which is known to be involved in the enzymatic destruction of catecholamines in the synaptic cleft. Secondly, many polyphenolic compounds protect cell membranes from destruction by free radicals that are formed in excess under the action of extreme factors and thereby significantly affect the course of the adaptation process. Apparently, they not so much themselves inhibit the processes of lipid peroxidation in the body, but reactivate the components of the natural non-enzymatic antioxidant system. The latter is represented by substances that, when oxidized, can break the free radical oxidation chain: tocopherols, ascorbic, citric, nicotinic acids, ubiquinone, phospholipids, steroid hormones, compounds having sulfhydryl groups, and some others. The interaction of polyphenolic adaptogens with a non-enzymatic antioxidant system is confirmed by some indirect data. In particular, lemongrass extract shows synergism with tocopherol, increasing the resistance of athletes to fatigue, optimizing their recovery processes and increasing specific power indicators. Thirdly, polyphenolic compounds realize their effects with the mandatory participation of the endocrine system, in particular the pituitary-adrenal system (Brekhman I.I., 1976; Meerson F.Z., Pshennikova MG, 1988; Lupandin A.V., 1991).

According to these ideas, polyphenolic adaptogens can be divided into three groups:

- with a predominantly neurotropic effect: lignans of Schisandra chinensis;

- with a predominantly antioxidant effect: flavonoids, catechins, leukoanthocyanides, proanthocyanides of cultivated grapes, as well as vitamins and vitamin-like substances contained in it;

- with a mixed action: green tea extract containing glycosides (caffeine, theophylline), providing a neurotropic effect, and catechins, vitamins and vitamin-like substances that mediate the antioxidant effect (Lupandin A.V., 1991).

According to the classical scheme of formation of individual adaptation developed by F.Z. Meerson, the correction of urgent adaptation by polyphenolic adaptogens is realized primarily due to the selective modulation of catecholaminergic synapses with subsequent restriction of the activation of the pituitary-adrenal system. Correction of long-term adaptation is achieved by reducing the intensity of lipid peroxidation and protecting tissues from destruction by free radicals (Meerson F.Z., Pshennikova MG, 1988). In this aspect, polyphenolic compounds (glycosides, lignans, flavonoids, etc.) can be of significant interest, which, without energy value or a specific action, increase the body's resistance to the action of a large number of extreme factors and their combinations, optimizing adaptation to these factors. Representatives may be compounds contained in known plants, in particular in Chinese magnolia vine, cultivated grapes, tea, and many others (Meerson F.Z., Pshennikova M.G., 1988; Lupandin A.V., 1991; Pilat T.L. ., Ivanov A.A., 2002).

The seeds of Schisandra chinensis contain essential oils, vitamin E. The main active ingredients are lignans: schizandrin, schisandrol, deoxyschisandrol (Muravyova D.A., 1991; Murray M.T., 1996).

Green tea extract contains alkaloids, caffeine, theophylline, tannins, catechins, vitamins C, B ₁ , B ₂ , K, PP, pantothenic acid (Muravyova D.A., 1991; Murray MT, 1996; Pilat T.L., Ivanov A.A., 2002).

Grape crest extract contains tannins, quercetin, glycosides, organic acids, vitamins C, groups B, K, folacin. The main active substances are catechins, proanthocyanides and leukoanthocyanides (Muravyova D.A., 1991; Murray M.T., 1996).

In the licorice extract as active substances, flavones (liquiquirozide) and glycoside glycyrrhizin are present, which are similar in structure to steroid hormones (Murray MT, 1996; Tutelyan V.A., Sukhanov B.P. et al., 1999; Pilate T. L. ., Ivanov A.A., 2002).

The present invention is the creation of a tonic composition based on plant components, which helps to accelerate the recovery process with depleting physical activity, improve psychomotor reactions and the activity of the cardiovascular system

The problem is solved as follows.

A composition having a tonic effect based on plant materials is proposed. The composition contains extracts of grapes, green tea, fruits of magnolia vine, licorice root and blueberries in the following ratio of ingredients, in wt.%:

grape comb extract 0.1-25 green tea extract 0.1-25 Schisandra fruit extract 0.1-25 licorice root extract 0.1-25 blueberry extract rest

The composition may be in the form of a powder, caramel, tablet, and may further contain targeted additives.

The composition is tentatively named "Bulldozer".

The composition can be used ready-made dry plant extracts produced according to TU 9370-027-02068315-02, or extracts prepared using an aqueous or aqueous-alcoholic extractant.

The composition is obtained by mixing the components (in liquid or dry form) in predetermined proportions.

The acute toxicity index (LD ₅₀ ) of the Bulldozer composition was studied by oral administration of 40 sexually mature white non-linear mice of both sexes weighing 18-22 g by the generally accepted method (Sernov L.N., Gatsura V.V., 2000). Animals were randomly divided into 4 groups of 10 animals. An aqueous solution was prepared from the test composition. The first group was administered intragastrally a solution of the studied composition at the rate of 2500 / mg kg, the second - 5000 mg / kg, the third - 7000 mg / kg. The fourth control group was injected with distilled water in the same volume. Animals were placed in separate cages with free access to food and water. Within 24 hours, the number of dead animals in each group was recorded. For the composition "Bulldozer" acute toxicity index LD ₅₀ > 7000 mg / kg

The pharmacological activity of the claimed complex was studied on volunteers.

In the study of pharmacological activity, the composition was taken in different variability of these ratios.

The research involved 83 Russian students studying at the Volgograd Medical Academy, aged 18-20 years. Students were randomly assigned to 3 groups. The first group (41 people) after exercise received the oral composition "Bulldozer". Representatives of the second group (12 people) received a placebo, and the 3rd group (30 people) was a control.

As a solvent for the composition "Bulldozer" for oral administration, a Karkade tea drink in an amount of 100 ml, cooled to a temperature of 37-40 ° C, was used. The original drink for breeding was prepared at the rate of 1 standard bag (2 g) per 100 ml of boiled water. In the case of preparing a placebo solution, the same amount of Karkade tea drink was used, cooled to a temperature of 37-40 ° C, with fructose dissolved in it, in an amount of 850 mg.

Physical activity lasting 30 minutes was performed under standard conditions (sports hall of the Volgograd Medical Academy, at a temperature of 18 ° C) in the following sequence:

1. Running around the perimeter of the gym - 1000 m, at a pace of 180 steps per minute. The pace of running was set by the electronic pace meter of walking and running "Rhythm and Rest". The time interval for rest after running was 1 minute.

2. Exercises with a gymnastic bench:

- squats with a gymnastic bench on the forearms when building in one line (6 people) 2 × 30 times. The main working muscle groups are the hip extensors. The operating mode is dynamic. Rest - 1 minute;

- lifting the bench up with both hands from each shoulder alternately - 2 × 30 times, standing in a column one at a time (6 people). The main working muscle groups are the extensors and flexors of the shoulder, the pectoralis major and minor, the middle bundles of the deltoid muscles. The operating mode is dynamic. Rest - 1 minute;

- flexion and extension of the arms in the elbow joints, holding the bench by the lower pole with a grip from the bottom - 2 × 30 times, when building in a row (6 people). The main working muscle groups are the flexors and extensors of the shoulder. The operating mode is dynamic. Rest - 1 minute;

- deep bends to the sides with a bench in hands 2 × 30 times when building into a column one at a time (6 people). The main working muscle groups are the lateral surface of the trunk. The operating mode is dynamic. Rest - 1 minute;

- flexion and extension of the arms with emphasis lying on the floor, legs on the bench 2 × 20 times. The main working muscle groups are the flexors and extensors of the shoulder, the pectoralis major and minor muscles, the front and middle bundles of the deltoid muscles, and the long back muscle. The operating mode is dynamic. Rest - 1 minute;

- from the starting position: one leg on the bench, the other on the floor, changing the position of the legs in a jump - 2 × 50 times. The main working muscle groups are the flexors and extensors of the thigh, lower leg, and ankle joint muscles. The operating mode is dynamic. Rest - 1 minute.

3. Running around the perimeter of the gym - 1000 m, at a pace of 180 steps per minute.

Subjective assessment of well-being by subjects before and after physical activity in% of the total number of people is shown in table 1.

Table 1.
SUBJECTIVE ASSESSMENT OF WELL-BEING BY THE EXPERIENCES IN% OF THE TOTAL NUMBER OF PEOPLE IN THE GROUP Assessment of well-being To load After loading Good 83.3 66.7 Satisfactorily 16.7 33.3 poorly 0 0

As can be seen from the data presented, not one of their subjects noted poor health.

The restoration of the functional state of the central nervous system, estimated by the time of an elementary motor reaction to a conditioned stimulus, is presented in table 2.

Table 2.
DYNAMICS OF CHANGES IN THE RESPONSE TIME TO THE IRRITANT AT CHRONOR REFLEXOMETRY OF THE TESTS AFTER PHYSICAL LOAD (M ± m) (ms) Group 5 'after loading 15 'after loading 30 'after loading 45 'after loading The control 19.20 ± 0.14 20.25 ± 0.53 18.58 ± 0.61 17.52 ± 0.82 Placebo 20.40 ± 1.35 20.15 ± 0.61 19.95 ± 1.03 18.58 ± 0.86 Bulldozer 19.19 ± 1.09 16.92 ± 0.82 * 16.97 ± 0.41 16.73 ± 0.72 * - P <0.05 with respect to control and placebo.

As can be seen from the data presented, in individuals taking the composition "Bulldozer", already at 15 minutes after exercise there was a restoration of the time of the elementary motor reaction to the conditioned stimulus to the initial level (before the load), while in the control group and placebo group recovery to the initial level was observed by the 45th minute after exercise.

The influence of the composition of "Bulldozer" on the process of restoring the physical performance of volunteers after exercise.

The process of restoring physical performance is presented in dynamics by the level of accuracy of muscle efforts and the results of the tapping test (Tables 3, 4 and 5).

Table 3.
DYNAMICS OF CHANGE IN ACCURACY OF MUSCULAR EFFORTS TESTED AFTER PHYSICAL LOAD (M ± m) (% deviation) Group 5 'after loading 15 'after loading 30 'after loading 45 'after loading The control 50.72 ± 10.55 52.59 ± 10.54 45.96 ± 9.42 48.0 ± 5.16 Placebo 53.22 ± 6.87 48.78 ± 11.44 44.93 + 8.41 45.78 ± 7.11 Bulldozer 51.30 ± 7.87 41.04 ± 9.46 36.96 ± 6.37 32.38 ± 5.52 * * - P <0.05 with respect to the control.

Table 4.
DYNAMICS OF CHANGES IN THE SPEED OF DOTING POINTS IN TEPPING TEST AFTER PHYSICAL LOAD (M ± m) (bpm) Group 5 'after loading 15 'after loading 30 'after loading 45 'after loading The control 5.43 ± 0.56 5.99 ± 0.67 6.17 ± 0.40 6.33 ± 0.64 Placebo 5.22 ± 0.37 5.73 ± 0.68 5.82 ± 0.58 5.70 ± 0.64 Bulldozer 5.59 ± 0.59 6.28 ± 0.55 6.54 ± 0.53 6.66 ± 0.47 Table 5.
DYNAMICS OF CHANGES IN THE TIRING FACTOR BY THE TEPPING TEST DATA AFTER PHYSICAL LOAD (M ± m) Group 5 'after loading 15 'after loading 30 'after loading 45 'after loading The control 0.85 ± 0.12 0.92 ± 0.08 0.9 ± 0.04 0.97 ± 0.04 Placebo 0.90 ± 0.04 0.90 ± 0.08 0.88 ± 0.02 0.98 ± 0.12 Bulldozer 0.90 ± 0.10 0.96 ± 0.05 1.03 ± 0.02 * 1.06 ± 0.12 * - P <0.05 with respect to control and placebo

The level of accuracy of muscle efforts in all groups at 1 minute after exercise was significantly reduced in relation to the outcome by an average of 42.9% in relation to the outcome (P <0.05) and gradually recovered by the 45th minute, however, the speed and the degree of recovery of this indicator in the studied groups was different. So, at the 15th minute of the recovery period, the difference with respect to the initial deviation of the accuracy of muscle effort in the control and placebo groups was 30.2% and 20.1%, respectively. In the group taking "Bulldozer", this indicator returned to its original value. At the 45th minute of the recovery period, while this indicator worsened at the level of 18.8% in the control and 13.3% in the placebo group, in the experimental group there was a significant improvement in the accuracy of muscle efforts by 19.9% (P <0.05 ) According to the results of the tapping test, the speed of applying the dots was approximately the same in all groups (Table 4), however, in the group taking "Bulldozer", the fatigue coefficient was restored to the initial values (according to the tapping test) already at the 30th minute of the recovery period (P <0.05), in contrast to the control and placebo groups, in which the value of the fatigue coefficient did not reach the initial level at the 45th minute of the indicated period (Table 5).

The data obtained during the measurement of the accuracy of muscle efforts and in the tapping test indicate an increase in the kinesthetic sensitivity and improvement of the coordination capabilities of the neuromuscular apparatus when taking the “Bulldozer” composition, which may be explained by the presence of a stimulating effect.

The studies were also carried out on the basis of the Russian Scientific Research Institute of Health by a research team, which included members of the scientific group established in accordance with the agreement on scientific and practical cooperation between the Russian Scientific Research Institute of Health and the National Scientific Center for Narcology of the Russian Ministry of Health.

During the tests, the participants, according to the scheme, took tonic drinks: Red Bull, Non-Stop, "Bulldozer" (powder) and a neutral carbonated drink, while at various stages of testing in the appropriate questionnaires they expressed their feelings in the emotional, cognitive and somatic spheres .

The studies were carried out using the hardware-software complex for assessing the psychophysiological state of a person "TONUS" (production of OOO Research Institute of Problems of Technologies and Intellectual Rights). The device is a prefix to an IBM compatible computer, interface, and software package in a Windows 98 environment.

1. Tapping test. This test allows you to determine the properties of the nervous system, such as lability and strength. The maximum possible frequency of pressing the right (leading) hand on the sensor located on the instrument panel with the index finger was determined. It is believed that the higher the frequency of clicks, the better the functional state of a person, his nervous system.

2. Tremorometry. In this study, tremor (trembling) of the leading hand was determined. The test subject had to hold the pin in the hole so that it did not touch its walls (when touched, the red light came on); while the elbow is in weight. This indicator reflects the level of regulation of the tone of peripheral nerves and muscles from the higher motor centers (cerebellum and motor zones of the cerebral cortex).

3. The time of a simple hand-eye reaction. This test, like the tapping test, reflects the state of the peripheral nervous system, but in this case, hand-eye coordination is also involved. This indicator reflects the signal detection time (the latent period from the moment the signal appears until the moment the device button is pressed) is recorded. The time value of a simple visual-motor reaction normally ranges from 160 to 220 ms. The shorter the reaction time, the better the functional state of a person.

4. The reaction time of choice (visual stimulus). A complex reaction, or a selection reaction, in contrast to a simple reaction, when executed, includes a decision-making step (a choice of two alternatives). Therefore, the reaction time of choice is on average longer than the time of a simple reaction and is about 300 ms.

5. The critical frequency of light flicker. The light signal repetition rate, at which they are perceived as a continuous signal, is called the critical light flicker frequency (CFSM). A series of light pulses is perceived as a continuous signal if the intervals between them are commensurate with the inertia time of the visual analyzer. CFSM is measured in the range from 14 to 70 Hz depending on brightness, stimulus shape, angular size of the object, projection onto the retina, level of adaptation of the visual receptors, and the functional state of the visual analyzer. With the improvement of the functional state of a person and, consequently, of his visual analyzer, the value of CFSM increases, and with deterioration, on the contrary, it decreases.

6. Short-term (operational) memory (for geometric shapes). RAM allows you to save current information for the time necessary to solve certain practical problems. In real conditions, this time can vary from a few seconds to several minutes. The amount of RAM is its most important characteristic and is determined by the number of correctly reproduced in a single presentation of the elements. This indicator is practically independent of the information content of incentives and averages from 5 to 9 units.

7. Switching attention. To study the properties of attention (attention switching time), the red-black table technique (Schulte-Platonov table) was used. The instruction to the subject consisted of the following: it is necessary to find (alternating) red numbers in decreasing order, starting at 13, and black ones in ascending order (starting from 1). The time taken to complete the task, the number of errors, and the average error correction time were recorded. The shorter the execution time and less errors, the better the concentration and switching attention.

The effect of taking the investigated drugs was felt by all subjects. According to their reports, the effect was manifested in an improvement in overall well-being, attention, the appearance of clarity of thinking, the elimination of muscle discomfort, eye fatigue, the appearance of vigor, and a decrease in drowsiness.

The severity of the effect after the first intake is the highest when drinking Red Bull, however, for other additives, the severity of the effect increases as a result of the second dose.

It should also be noted that after taking Non-Stop and Red Bull caffeinated drinks, individual subjects noted a deterioration in the flow of thought processes (“inhibition of thought and action,” “the head thinks worse, but I perform the actions clearly”).

The results obtained are consistent with the notion that drugs with adaptogenic effects obtained from plants have a slight psychostimulating effect (in terms of severity and speed of development, they are significantly inferior to psychostimulants and general tonic drugs). Psychostimulation is manifested in improving performance (physical and mental), reducing signs of asthenia and fatigue. This effect is not accompanied by signs of arousal, including euphoria. It should be noted that the manifestation of the general tonic and psychostimulating effects of "energy" drinks is in the range of physiological norms.

Adaptogens, including those included in the studied drinks, have a complex chemical structure and their effect cannot be explained due to any one of the components. In addition, when preparing a mixture of additives for many tonic agents, substances are selected so that they enhance the effects of each other. Polyphenolic plant adaptogens (extracts of eleutherococcus, leuzea, rhodiola, lemongrass), as well as vitamins E and C belong to the group of antioxidants and antihypoxants that protect the body (its cells and tissues) from the damaging effects of free radicals and oxygen deficiency.

Vitamins added to almost every tonic drink by themselves do not cause an increase in working capacity, but can be considered as factors contributing to the recovery processes, especially in combination with adaptogens and antioxidants.

It is assumed that in the implementation of adaptogenic action also plays a role in enhancing the adaptive synthesis of RNA and proteins, the activity of energy metabolism enzymes and regeneration processes.

The general tonic effect of the studied drugs develops gradually and is expressed in an increase in the tone and vital activity of the body, there is a restoration of reduced vascular tone, a slight increase in blood pressure and heart function. It is believed that the basis of these effects is the activation of metabolism, endocrine and autonomic regulation.

The results of the study show that the claimed tool is not toxic. The complex quickly removes the feeling of fatigue in various situations, increases working capacity, endurance, adjustment of attention, improves eyesight and speed of reaction in emerging stressful situations. The complex has a positive effect on the quality of performing complex operational tasks, positively affects the activity of the cardiovascular system, improves mood.

When studying tests on healthy volunteers conducted at the Volgograd Medical Academy Department of Physical Education, the effect of the composition “Bulldozer” on the psychophysiological status of the subjects, subjective condition, physical performance, quality of operant activity, as well as indicators of the state of the cardiorespiratory system before and after physical activity was studied .

It was established that the Bulldozer complex of biologically active substances has a general tonic effect, improves the subjective positive perception of the severity of physical activity, reduces the manifestations of fatigue, which is manifested by an increase in the quality of the corrective test, restores kinesthetic sensitivity (according to the measurement of the reproduction of the dosed muscle effort), improves response time to an external stimulus (according to chronoreflexometry).

The studied compound does not affect the parameters of cardiovascular activity, causes a statistically insignificant increase in oxygen demand (according to the data of a breath-holding test (Genchi test) and in some cases affects the quality of sleep).

The study on volunteers found that after exhausting physical activity, the composition of the biologically active substances "Bulldozer" improves the subjective positive perception of significant physical activity, reduces manifestations of fatigue (according to the tapping test), restores kinesthetic sensitivity, increasing the accuracy of muscle efforts, increases mental performance by exerting positive dynamics on the quality of performing complex operant tasks ("Corrective Test"), and also objectively improves the condition Aries reflex response to an external stimulus.

Claims (3)

1. A composition having a tonic effect on the basis of plant materials, characterized in that it contains extracts of grapes, green tea, fruits of magnolia vine, licorice root and blueberries in the following ratio of ingredients, wt.%: Grape Comb Extract 0.1-25 Green tea extract 0.1-25 Schisandra fruit extract 0.1-25 Licorice root extract 0.1-25 Blueberry extract Rest 2. The composition according to claim 1 is made in the form of a powder, caramel, tablet, solution, drink. 3. The composition according to claim 2 further comprises targeted additives.