RU2287301C1 - Food biologically active additive of antitoxic properties - Google Patents

Abstract

FIELD: food industry, butter and fats industry.

SUBSTANCE: the present innovation deals with obtaining high-quality biologically active additive (BAA) applied for simultaneous intake with food and, also, in case of cellular lesions caused with toxic factors. Food BAA suggested includes vegetable oil or the mixture of vegetable oils and CO₂-extract. As CO₂-extract it is necessary to apply CO₂-extract of yarrow at the quantity being not more than 5 weight%. Vegetable oil or the mixture of vegetable oils and CO₂-extract should be additionally treated in constant electromagnetic field at magnetic induction being about 0.45-0.70 Tl at flow rate being 1.0-2.5 m/sec for about 15-40 min at 20-25° C. The innovation enables to obtain food BAA of high antitoxic action. It is highly efficient at alcoholic and other types of intoxication, chronic radiation disease and hepatotropic intoxications.

EFFECT: higher efficiency of application.

4 ex, 1 tbl

Description

The invention relates to food, namely to the oil and fat industry, and can be used to obtain high-quality biologically active additives (BAA), used for direct consumption in food, as well as in case of cellular damage caused by toxic factors (alcohol intoxication, chronic radiation sickness, hepatotropic poisoning).

It is known that polyunsaturated acids (SCFA) of fish oil are used in diet therapy. / Samsonov M.A., Isaev V.A. New in the prevention and treatment of atherosclerosis, coronary heart disease, hyperlipidemia and other diseases. - Nutrition issues. - No. 4. - 1995, - S.33-34 /. Their disadvantage is that they initiate free radical oxidation (SRO) of lipids in the body, the excessive acceleration of which inevitably damages the cell membranes, disrupting the metabolism in the cells of the body.

Also known is the pharmaceutical preparation "Aevit" - a 50% oil solution of vitamins A and E (in a 1: 1 ratio), intended for the treatment of atherosclerotic changes in blood vessels, tissue trophic disorders, obliterating endarteritis.

The disadvantage of the drug is the danger of hypervitaminosis in case of overdose, and with increased sensitivity to vitamins and at usual dosages.

Closest to the claimed is sunflower oil "Steppe aroma" with additives of carbon dioxide extracts of spices and aromatic plants (thyme, saffron, parsley, coriander) ("Steppe aroma" according to TU 10-480134-33-92 - prototype).

The disadvantages of this oil are:

- unrefined vegetable oil is a source of peroxide compounds that initiate free-radical oxidation of lipids in the body, the latter leads to a violation of lipid metabolism in the body, and therefore to disorders responsible for the development of atherosclerosis;

- the functional orientation of the product is insufficient for nutrition in lipid metabolic disorders due to the lack of an optimal ratio of linolenic and linoleic acids in its composition, the physiological effects of which in the body are caused by arachidonic and eicosopentaenoic acids formed as a result of elongation and desaturation.

The objective of the invention is the creation of dietary supplement for food with antitoxic properties in cell damage caused by toxic factors.

The problem is solved in that the biologically active food additive consisting of a vegetable oil or a mixture thereof and CO ₂ extracts, as a CO ₂ extract contains CO ₂ extract of yarrow in an amount of not more than 5 wt.%, And vegetable oil, or the mixture and the CO ₂ extract are additionally treated in a constant electromagnetic field with magnetic induction of 0.45-0.70 T at a flow rate of 1.0-2.5 m / s for 15-40 minutes at a temperature of 20-25 ° C.

The inventive dietary supplement has the indicated properties, probably due to the following.

As we have shown experimentally, the mechanisms of the therapeutic effect of a new product of the claimed composition are reduced, on the one hand, to enhancing the function of the antioxidant system of the body and, on the other hand, to providing the body with plastic material to restore the integrity and functional activity of cell membranes affected by the pathological process.

The main biologically active principle in the antitoxic effect of the product is a complex of PUFA and phospholipids, obtained by new technology. The inventive process parameters (the magnitude of the magnetic induction of a constant electromagnetic field, flow rate, time of formation of the system) ensure the conservation of the biological activity of native plant phospholipids and PUFAs.

Vegetable oils included in dietary supplements are represented by two-component mixtures balanced by PUFAs of the ω-3 family and the ω-6 family, the biological role of which is due to the participation in the structural and functional organization of cell membranes.

The optimal amount and ratio of these PUFA fractions in dietary supplements corrects the structure of cell membranes, which is extremely important in terms of their permeability and stability. By regulating the processes of free-radical oxidation, dietary supplement helps to restore the chemical composition, structure and function of cell biomembranes and their susceptibility to regulatory influences.

As we have shown experimentally, the use of a constant electromagnetic field with the indicated parameters at the stage of mixing the CO ₂ extract with vegetable oil under mild temperature conditions leads to a change in the structure of molecules, weakening or even partial breaking of hydrogen or coordination bonds, which increases the polarity of polyunsaturated fatty acids and phospholipids contained in CO ₂ extracts.

Thus, the needs for omega-3 PUFAs are met both by directly incorporating the fatty acids entering the dietary supplement into the lipids of the cell membranes and by metabolism along the entire chain — elongation and desaturation of the starting linolenic acid into polyunsaturated analogues. This avoids the simultaneous circulation in the bloodstream of a significant amount of PUFAs, which are a substrate of peroxidation in the body.

The use in the proposed dietary supplement complex of phospholipids with high-quality triacylglycerols increases the "degree of unsaturation" of cell membranes and the permeability of the latter by 20-30% on average. At the same time, the evacuation of toxic substances (decomposition products of unsaturated fatty acids, which are part of cell membranes damaged by free radical oxidation, as well as phospholipid complexes with heavy metal ions) from the body is facilitated.

The phospholipid-triacylglycerol complex of plant origin has a beneficial effect on phospholipid metabolism in platelet membranes. Moreover, as we have shown experimentally, the content of readily oxidizable phospholipid fractions (phosphatidylserine, phosphatidylethanolamine) responsible for the interaction with heavy metal ions, in combination with an increase in the amount of structural stabilizer of sphingomyelin cell membranes, enhancing the antioxidant potential of the lysosomal layer, significantly increases.

The additional introduction of a biologically active component such as yarrow extract containing vitamin E, K and beta-carotene into the dietary supplement in the claimed amount provides a synergistic effect on the antitoxic properties of the new product, since they are interceptors of free radicals when initiating free radical lipid oxidation processes, which accompanies any pathological processes associated with damage to cell membranes.

Thus, the claimed dietary supplement is a complex of biologically active components, the inclusion of which in the diet prevents the destruction of cell membranes, partially restoring cellular structures.

The antitoxic activity of the claimed dietary supplement to food is confirmed by examples, the initial indicators of which are technological indicators characterizing the parameters of the constant electromagnetic field, the duration and temperature of the processing system, as well as the claimed composition of the product. The following indicators were experimentally determined:

the activity of lysosomal (surrounded by a membrane) enzymes (aryl sulfatase, beta-galactidase) as an indicator of the ability of a product to influence the state of liver membranes and change their properties;

lipid peroxidation (LPO) activity was evaluated by the content of malondialdehyde (MAD);

erythrocyte hemolysis characterized the latter's resistance to hydrogen peroxide and the degree of their regeneration (recovery) by replacing damaged PUFAs in the erythrocyte membrane with undamaged ones received with the product.

As a destroyer of cell membranes, the mycotoxic product of the vital activity of mold fungi - T-2 toxin was used.

The experiments were carried out at the Institute of Nutrition RAMS (Moscow) on growing white rats of the Wistar line, in the diets of which were introduced the claimed dietary supplements, complete in all irreplaceable components (experimental groups of only 4, 15 animals each) and a well-known product (control group, 15 animals), consisting of unrefined sunflower oil and aromatic plant extracts. Products were included in rat diets in the amount of 30% of the fatty part of the diet.

T-2 toxin was administered to animals that received a pre-4 week control or experimental rations with the claimed product in accordance with the examples. T-2 toxin was administered orally in a 0.1% aqueous solution at a dose of 1 mg / kg for 9 days. Then the animals were killed and conducted the studies below.

The manifestation of the toxic effect of T-2 toxin - loss of appetite, ruffle, lethargy, gemmarogical exudates around the nose - was observed in animals of all groups, and their severity was the same, as well as a slowdown in body weight gain compared to rats that were not exposed to seeding. For the entire period of the experiment there were no cases of death of animals. At autopsy, it was found that the relative liver mass in rats receiving T-2 toxin against the background of the control diet was 60% higher than in intact (without seed), and increased only by 40% against the background of the experimental diet. The liver of all rats treated with the toxin and control diet was greatly enlarged and had a pronounced fatty infiltration upon microscopic examination. In the group of rats treated with toxin and experimental diets, moderate rats had a moderate fatty infiltration in 2 out of 15 rats. In the remaining animals of these groups, only the initial forms of fatty liver infiltration were noted.

The inventive dietary supplement to food is illustrated by examples.

Example 1

Food supplement contains sunflower high oleic refined deodorized oil (50.54%), linseed unrefined oil (44.46%) and a yarrow CO ₂ extract processed by mixing with a mixture of vegetable oils in a constant electromagnetic field with magnetic induction of 0.70 T at a flow velocity of 2.4 m / s for 25 min at a temperature of 22 ° C - 5%.

Example 2

Food supplements contain rapeseed refined deodorized oil (38.8%), soybean refined deodorized oil (58.2%) and a yarrow CO ₂ extract treated with a mixture of vegetable oils in a constant electromagnetic field with magnetic induction of 0.45 T at a flow velocity of 1.5 m / s for 30 min at a temperature of 25 ° C - 3%.

Example 3

Supplements for food contain refined corn deodorized oil (95.0%) and a yarrow CO ₂ extract processed by mixing with refined corn deodorized oil in a constant electromagnetic field with magnetic induction of 0.55 T at a flow velocity of 1.6 m / s in for 35 minutes at a temperature of 20 ° C - 5%.

Example 4

Food supplements contain sunflower high oleic refined deodorized oil (77.62%), corn refined deodorized oil (21.38%) and a yarrow CO ₂ extract, processed by mixing with a mixture of vegetable oils in a constant electromagnetic field with magnetic induction of 0.60 T at a flow rate of 1.0 m / s for 15 min at a temperature of 25 ° C - 1%.

The values of the studied indicators are given in the table.

As can be seen from the table, the claimed dietary supplement has antitoxic properties, since with direct use the liver is less affected by toxic effects, which is confirmed by pathological studies and the protective effect of the claimed dietary supplement on rat liver lysosomal membranes (the enzyme system of lysosomes is activated).

Name of indicator The inventive dietary supplement to food Famous product example 1 example 2 example 3 example 4 one 2 3 four 5 6 Non-sedimentary activity (% of total) of rat liver lysosome enzymes: arylsulfatase 4.01 3.83 3.91 3.78 1.97 beta galactidase 5.34 3.89 3.60 3.30 2.10 MDA, nmol / ml 301,2 284.7 268.3 251.2 362.2 Peroxide hemolysis erythrocytes,% 18.6 18.8 20.3 19,4 33.8

The use of the claimed dietary supplement in a diet had a protective effect on the activation of LPO in the liver, intensified by the toxin. With the direct use of the claimed dietary supplement, the stability of erythrocyte membranes increases due to the exchange of damaged phospholipid molecules into intact biologically active ones from a new product. Known product does not have a membrane protective effect.

Thus, the claimed dietary supplement to food is active as a protector of toxic effects caused by toxins, in the mechanisms of the destructive action of which lies damage to the structural and functional organization of cell membranes.

The claimed dietary supplement, having an antitoxic effect, can be recommended for direct use in food and diet of patients with alcohol and other intoxication, chronic radiation sickness, hepatotropic poisoning.

Claims (1)

Dietary supplement to food, consisting of a vegetable oil or a mixture thereof and CO ₂ -extract, characterized in that the CO ₂ extract of CO ₂ used yarrow extract in an amount of not more than 5 wt.%, And vegetable oil or a mixture thereof and the CO ₂ extract is additionally treated in a constant electromagnetic field with magnetic induction of 0.45-0.70 T at a flow rate of 1.0-2.5 m / s for 15-40 minutes at a temperature of 20-25 ° C.