RU2272630C2 - Method for increasing cow embryo survival - Google Patents

Abstract

FIELD: veterinary science.

SUBSTANCE: method for increasing cow embryos survival involves a single intramuscular administration gonadoliberin surfagon in the dose 10 mcg to cows on 8-10 days after insemination followed by additional injection of thymogen in the dose 5 mcg/kg of body mass on 8, 9 and 10 days. Method provides maintenance of hormonal activity of yellow body in cows.

EFFECT: improved increasing method.

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Description

The invention relates to agriculture, in particular to livestock, and can be used to prevent embryonic mortality in female cattle.

In experiments with the slaughter of animals 2-3 days after insemination, it was found that fertilization occurs in 95-100% of cases. Not more than 50-70% become pregnant from insemination in one hunt. The difference between fertility and the percentage of pregnancy is embryonic loss. Most of these losses occur due to the death of zygotes, morula and early blastocysts in the first week of embryogenesis, and late blastocysts and early embryos - in the initial period of implantation - from 8 to 22 days.

The causes of embryonic mortality in clinically healthy cows are very diverse. Among them is the inadequate state of the animal immune system during critical periods of embryogenesis, arising from the immunosuppressive effect of lactation on the body.

A known method of reducing the loss of embryos in cows, based on the use of progesterone. An oil solution of the hormone in a dose of 100 mg is administered 3, 5, 7 and 9 days after insemination (Ustinov A.P. Effect of progesterone on fetal mortality in cows // Tr. All-Russian Research Institute of Beef Cattle Breeding, 1970. - Issue 15).

A known method of activating the corpus luteum in cows by intramuscular injection of surphagon at a dose of 10 μg. Deciphering the chemical structure and subsequent synthesis of gonadoliberin made it possible to use it to increase the hormonal activity of the corpus luteum in order to maintain and preserve pregnancy (VIDAL Reference book, veterinarian. Medicines for veterinary use in Russia. - M.: AstraFarmService, 2003/2004 - 480 p.) .

The disadvantage of these methods is that the effectiveness of the use of progesterone and surphagon largely depends on the individual sensitivity and condition of the target cells.

The aim of the invention is to increase the survival of embryos in cows.

This is achieved by the fact that animals are administered an immunomodulator at 8, 9, and 10 days after insemination. For 8-10 days, the introduction of an immunomodulator is combined with a single injection of gonadoliberin.

The immunomodulator thymogen, administered to cows at a dose of 5 μg / kg, has a corrective effect on the immune system of animals. Gonadoliberin Surfagon at a dose of 10 μg activates the synthesis of lutropin and its entry into the bloodstream, which contributes to an increase in the functional activity of the corpus luteum. The use of biologically active substances according to this scheme ensures the maintenance and progression of pregnancy in the initial period of implantation.

Thus, the technical result of the claimed invention is to increase the survival of embryos in cows.

Example. An experiment was carried out on crossbred (PE × G) cows of 3-5 lactation, with a productivity of 4750-5000 kg per lactation. After obstetric and gynecological examination on 45-50 days after calving, three groups of clinically healthy animals were formed: two experimental of 15 and 16 goals and one control of 23 goals. Cows of the first experimental group were injected intramuscularly at a dose of 10 μg intramuscularly on the 8th day after insemination, the second - surfagon (8 days, 10 μg) + thymogen (8, 9, 10 days, 5 μg kg / mass). Animals of the third group served as a control, they were injected with 2 ml of isotonic sodium chloride solution. The effectiveness of the drugs was evaluated by the onset of pregnancy in cows, determined by rectal palpation 50-60 days after insemination, the insemination index and the duration of the service period.

The research results are shown in the table.

1. The effect of the combined use of an immunomodulator and gonadoliberin on the survival of embryos in cows

Indicators Groups First (n = 15) The second (n = 16) Third (n = 2) They became pregnant after two inseminations: n eleven 13 fifteen % 73.3 81.3 652 Insemination index 2,5 2,3 3,1 Service period 84.5 83.3 98.1

As can be seen from the data presented in the table, after the administration of biologically active substances to the cows in the group of animals treated with thymogen together with the surrogate, 8.0% more pregnant became pregnant than in the group of animals that were injected with surfactant only. In addition, in this group, insemination was spent on 0.2 insemination less, and the service period was shorter by 1.2 days compared with the first group.

Thus, the combined use of an immunomodulator and gonadoliberin helps maintain the hormonal activity of the corpus luteum, which increases embryo survival and pregnancy.

Claims (1)

A method of increasing the survival of embryos in cows, including a single intramuscular injection of surfagon gonadoliberin in cows at a dose of 10 μg for 8-10 days after insemination, characterized in that the immunomodulator thymogen is additionally injected into the cows for 8, 9, 10 days at a dose of 5 μg / kg of body weight .