RU2271841C1 - Method for carrying out resonance-based repair of human immune status - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: method involves concurrently treating pulmonary blood circulation zone and reticular tissue of flat patient sternum bones with nonthermal microwave frequency radiation with resonant frequencies selected from bandwidth of 2-40 GHz and radiation power density of not greater than 1 mW/cm². Alternating magnetic field of 10-100 Hz and magnetic intensity amplitude to 50 mTesla units is applied for 90 min.

EFFECT: enhanced effectiveness of treatment.

5 cl, 6 tbl

Description

The invention relates to medicine and is intended to restore a violated immune status of a person in various immunodeficiency states caused by adverse environmental influences and / or pathogenic microorganisms, including HIV infection.

An immunodeficiency state is understood as the absence of one or several components of the immune system or the loss of their effectiveness due to various infectious diseases or adverse environmental influences (radiation, chemical xenobiotics, stress, etc.) [1].

In the 90s, a special class of substances was identified - immunomodulators [2, 3], which had high hopes, but they did not materialize. It is known [4, 5] that the same immunomodulators, depending on the dose, time, administration schedule and previous immunological history of the patient, can have both stimulating and suppressive effects, which greatly complicates the individual clinical selection of immunomodulators and subsequent treatment.

In addition, a number of side effects were identified associated with serious disorders in the functioning of the neuroendocrine system [6, 7, 8], including in AIDS patients [9, 10].

Studies on patent and medical literature have revealed a number of methods for the treatment and prevention of various diseases associated with exposure to magnetic and electromagnetic fields [11, 12, 13, 14], which significantly increase the effectiveness of treatment and prevention. All this is more than relevant against the backdrop of a deteriorating environmental situation, causing an increase in immunodeficiency diseases, as well as the onset of the AIDS pandemic.

A known method of EHF-therapy [15], including exposure to coherent EHF-radiation in the range of 30-300 GHz. However, this method is limited in functionality and technically complicated.

There is also a method of objective and non-invasive selection of the optimal frequency of electromagnetic radiation for EHF therapy [16], which is characterized by the indirect effect of EHF radiation on the studied cells and is multi-stage in nature. This effect greatly complicates the application of the method in clinical practice.

A known method of treating neoplasms and viral diseases [17] using electromagnetic waves in the frequency range 0.01-18 GHz with a power flux density of less than 10 mW / cm ² . The method was tested only on diseases associated with the etiology of acute respiratory infections and herpes. There is no experimental and clinical picture of the effect of HIV on this method and on the human immune status.

There is also known a method of magnetoelectromagnetic cleaning of an HIV-infected human body from AIDS virions [18]. But this method does not activate or regulate the human immune system.

As a prototype of the proposed method of resonant restoration of the human immune system, the method of magnetoelectronic reparative prevention of diseases of old age is selected [19].

The prototype method is designed only for exposure to electromagnetic radiation on stellate cells of the bone marrow reticular tissue and does not apply to diseases of viral etiology.

The objective of the invention is the creation of an effective, safe, easy-to-use outpatient method that allows you to quickly restore the violated immune status of the human body after suffering immunodeficiency diseases associated with the harmful effects of the environment and / or infection by pathogenic microorganisms of viral etiology, including HIV infection .

The technical result achieved by the implementation of the claimed invention is to restore the normal functioning of the bone marrow reticular tissue (RTKM) and suppress the replication of microorganisms of viral etiology, including HIV infection.

The problem is solved in that in the inventive method for the resonant restoration of the violated immune status of a person according to the invention, sessions of simultaneous exposure of the region of the pulmonary circulation and the reticular tissue of the sternum flat bones with non-thermal microwave radiation (UHF) with resonant frequencies (RF) selected from the range of 2- 40 GHz, with a power flux density of up to 1 mW / cm ² and an alternating magnetic field (PEMP) with a frequency of 10-100 Hz and an amplitude of magnetic induction of up to 50 mT for 90 min. The full course of restoration of the body’s immune system and / or complete reduction of the viral load for HIV infection consists of 8-11 outpatient non-invasive sessions of simultaneous exposure to UHF and PEMP, which are carried out sequentially every other day.

It is known that the molecular structure of water is an equilibrium mixture of hexagonal triatomic molecules associated through moving hydrogen bonds into openwork spatial formation [20]. In bioobjects, carbohydrates, interacting with water, form similar microcomplexes, which are cluster structures that behave like anisotropic liquids with signs of ordering. Being in a constant vibrational state, they form a system of oscillators, which, self-synchronizing, create in the microbiosystem (cell, protein system, etc.) their own weak electromagnetic field, inherent only to this biosystem.

It is known that RTKM consists of 65-70% of water and has cluster structures with the above properties [21]. Since RTKM is the main producer of blood cells, including lymphocytes - the basis of immunity, we can talk about the regulation of the immune system of the human body as a whole through the external influence of various fields on RTKM.

It is known that the cell-wall and nuclear proteins of the AIDS virion [22] form a tertiary structure due to ionic interactions of oppositely charged groups NH ₃ ⁺ and COO ^- . Such a structure is similar to the RTKM cell cluster and has a large number of oscillating dipoles, which lead to the appearance of intrinsic electromagnetic fields in the AIDS virion [23]. Obviously, in this case these fields can be controlled externally.

To select an algorithm for the implementation of the proposed method for resonant restoration of the violated human immune status, studies are carried out in two stages: the first is carried out in vitro, and the second in vivo.

The first stage is as follows:

1. Determine the resonant frequencies (RF) of the UHF radiation in the experimental samples (OP), choosing them from the range of 2-40 GHz with a scan step of 150 MHz with a frequency stability of no worse than 10 ^-2 . In this case, the density of the power flow of the UHF is set to not more than 1.0 mW / cm ² , which corresponds to the International standards for electromagnetic safety.

As the generator of the UHF radiation, a standard tunable Ghana diode with a power amplifier UM-412 is used.

2. RF search is carried out by the known method of resonance-transmission microwave radio spectroscopy with registration of the radio response of the sample using a highly sensitive (10 ^-17 W) microwave radiometer. At the same time, the interaction of external radiation with its own electromagnetic oscillation of clusters of stellate cells of RTKM or proteins of AIDS virions is observed.

3. Choose the optimal parameters of the amplitude of the magnetic induction of PEMP, which provides the maximum biological effect. A MAG-30-03 magnetic inductor with a pulse shaper FIS-01 is used as a PEMP generator. It was found that the increase in the bioeffect ceases when the magnetic induction amplitude of 50 mT is reached.

4. Select the required time of exposure to the resonant UHF-radiation and PEMP on RTKM stellate cells and AIDS virions, due to the end of the biological effect, which corresponds to 90 minutes.

5. The clinical efficacy of the combined effects of resonant UHF-radiation and PEMP on RTKM stellate cells and AIDS virions in working samples (RP) in vitro is evaluated.

For this, control samples (KO) selected from RP are irradiated for 90 min, constituting an exposure session. Sessions are held every other day. The latter is associated with the inertia of the biological response of the samples, which is observed during the day.

6. Sessions on exposure to KO are carried out until the expected clinical effect is confirmed. For RTKM - complete restoration of the normal structure of stellate cells. For AIDS virions - slowing down their replication and complete destruction. The latter is confirmed by the analysis of microprobe (MP) periodically withdrawn from KO. The final number of sessions will be the full course of exposure.

The first stage of research is implemented as follows. 260 RT-1 were sequentially selected from RTKM with stellate cell pathology. The same selection is carried out from lymphatic tissue infected with HIV infection, a total of 260 OP-2.

RTKM is placed in Earl's nutrient medium, and lymph tissue is placed in the nutrient formulation R-199, to which interleukin-2 is added. The storage temperature is maintained with a thermostat at + 3 ° C. The latter contributes to the long-term storage of the test material. Ditches in which OP-1 and OP-2 are irradiated are made of polyurethane plastic, which is close in electromagnetic and magnetic permeability to human biological tissue.

The impact of fields on the OP-1 and OP-2 is carried out at two workplaces. At the moment of coincidence of the internal electromagnetic oscillation of RTKM stellate cells or AIDS virions with the frequency of external LEL radiation, a resonance is observed, which manifests itself as an increase in the amplitude of the signal received by the microwave radiometer. In this case, the response of the biological object is recorded in the form of an amplitude-frequency dependence (resonance spectrum). In this case, the following parameters have information value: the amplitude of the resonant signal (∑ ₀ ), the resonant frequency of the signal (ν _p ) and the quality factor (Q), which is understood as the ratio of the resonant frequency of the signal to the half-width (δ _0.5 ) of the spectral line by level half amplitudes, i.e.

The radio response to the effect of resonant LFH radiation occurs as a result of the dissipation of resonant waves on the structural inhomogeneities of the clusters. Therefore, the amplitude of the radio response (∑ ₀ ) reflects the degree of structural disorder of the microbiosystem and is directly proportional to the entropy of the system. The latter can be used to modulate destructive effects on this biosystem.

The resonant frequency (ν _p ) reflects the strength and nature of intermolecular interactions, which can either be strengthened or weakened. The quality factor (Q) determines the degree of synchronization of the electromagnetic oscillations of oscillators in RTKM stellate cells and AIDS virions. It is used to select the most profitable and effective RF LFM radiation in OP-1 and OP-2.

After the detection and selection of RF, the clinical efficacy of the effect of radiation on biological samples taken from RTKM and lymphatic tissue is evaluated. For this, working samples (RP) are formed, which are not exposed to radiation, but are used as basic test benchmarks. Control samples (KO) were taken from RP and subjected to repeated irradiation. Each irradiation session is, as for OP, 90 minutes. The number of sessions is increasing. After each combined effect of resonant LFM radiation and PEMP on KO, micro samples (MP) are taken from them, which are analyzed. For MP-1 taken from RTKM KO, the analysis is carried out by the standard method of phase-contrast photomicroscopy (PCFM) with direct photocounting of cells. The result is statistical in nature with a probability of deviation of P = 0.05 and is calculated by the formula

where α is the coefficient of the regenerating effect, the dimensionless;

MP - the specific content in MP of deformed and damaged RTKM stellate cells after an exposure session, ed .;

RP - specific content in RP of deformed and damaged RTKM stellate cells. The value of the reference constant, from unit

The calculation results are shown in table 1.

For MP-2 taken from CDs of HIV-infected lymphatic tissue, the analysis is carried out by polymerase testing. To do this, use the Amplicor polymerase chain reaction (PCR) test system of the Hoffman-La-Roche company. Exposure sessions are carried out until they obtain the desired sustained clinical effect. It consists in the fact that in the last MP-1, a complete restoration of the normal structure of RTKM stellate cells is observed (Table 1), and in the last MP-2, the absence of AIDS virions is observed (Table 2). After achieving such a clinical effect, the effect on KO is stopped. The total number of sessions is the full course of exposure.

At the end of the first stage of research carried out in vitro, proceed to the second - in vivo. The algorithm of the second stage of the proposed method of resonant restoration of the violated human immune status is as follows:

1. Two groups of volunteers (volunteers) are selected. The first group (A) consists of 50 people who have immunodeficiency states caused by various reasons. The second group (B) consists of 10 people with an “experience” of HIV infection from 3 to 5 years and a T-4 content of heller lymphocytes (CD-4 ⁺ ) of at least 200 cells / μl.

2. Carry out simultaneous exposure to the resonant UHF-radiation and PEMP for each volunteer, using the previously selected parameters of these fields.

For this, an experimental apparatus is used, consisting of a generator of low-frequency radiation and a PEMP magnetic inductor, as well as auxiliary elements - a power meter, a ferrite gate, a waveguide path, an attenuator, etc.) [25].

3. Determine the clinical effectiveness of the method. For this, at the end of the full course of exposure, volunteers from both groups take blood tests. In group A, the results shown in the detailed immunograms are examined and systematized. In group B, in addition, the results of the PPR testing are analyzed. Based on the averaged results of immunocorrection in groups, a conclusion is drawn on the degree of clinical effectiveness of the method.

The second stage of the proposed method for the resonant restoration of the violated immune status of a person is as follows.

Group A is divided into 5 subgroups on the basis of immunodeficiency. These signs include:

- for subgroup A-1 - thermal injuries;

- for subgroup A-2, post-infectious complications of various viral etiologies (excluding HIV);

- for subgroup A-3 - post-stress state;

- for subgroup A-4 - transferred effects of exposure to ionizing radiation and chemical xenobiotics;

- for subgroup A-5 - the transferred consequences of an autoimmune disease (rheumatoid arthritis).

For all subgroups of group A, the degree of initial immunodeficiency state of volunteers is assessed as moderate.

For group B, the degree of disease of the volunteers is estimated as 2B-3A, which also corresponds to the degree of moderate severity.

For volunteers of all groups, the same conditions and sequence of operations of the proposed method for the resonant restoration of the broken immune status of a person are used.

Two PEMP magnetic inductors with an area of 108 cm ² each are fixed with elastic tape on the lateral surfaces of the anterior part of the sternum of patients.

A phased array (emitter of resonant low-frequency microwave radiation) with an area of 200 cm ^{2 is} fixed above the solar plexus region. With each subsequent session, the position of the inductors and emitter is reversed clockwise. Thus, a combined circle of blood circulation and RTKM flat bones of the anterior part of the sternum are subjected to the combined effects of the fields.

The full course of exposure to groups A and B consists of 8-11 hour and a half sessions, repeated every other day for 2-3 weeks. The course of exposure is carried out on an outpatient and non-invasive basis. The results of a clinical study in each group systematize and evaluate their effectiveness (Table 3-6).

So, for example, subgroup A-1 is characterized by effective immunocorrection, which resulted in a rapid transition of the immune system from a state of acute toxemia to normal, bypassing the period of septicotoxemia (Table 3).

For subgroups A-2, A-3, a stable remission is observed, consisting in a prolonged prolongation of the clinical effect after a course of exposure (Table 4).

Subgroup A-4 is characterized by rapid relaxation of the cellular component in the immune status of patients (Table 5).

In subgroup A-5, a positive mutual correction of the cellular and humoral levels of the immune system is observed (Table 6).

In subgroup B, there is a complete absence of AIDS virions in PCR test samples, as well as rapid positive immunocorrection of all levels of immunity.

Thus, the claimed method of resonant restoration of the violated immune status of a person is effective, safe and easy to use.

Information sources

1. Immunodeficiency conditions. Ed. prof. B.C. Smirnova and prof. I.S. Freidlin. The Tome, 2000.

2. Ivanov V.P. Immunomodulating peptides: the role of peptide fragments of endogenous and exogenous proteins in the modulation of immune processes. Successes of Modern Biology, 1994, vol. 114, issue 3, p. 353-371.

3. Novikov B.C., Smirnov B.C. Immunophysiology of extreme conditions. SPb., "Science", 1995, p.172

4. Korestelev S.A., Isakova K.L. et al. Effect of endogenous opioid peptides and their synthetic analogues on T-cell immunity. Experimental and Clinical Pharmacology, 1994, vol. 57, No. 1, pp. 57-61.

5. Miroshnichenko I.V., Sharova N.I., Ryabinina I.D. et al. Analysis of the biological activity of thymogen and synthetic analogues of thymopentin. Immunology, 1997, No. 2, pp. 25-29.

6. Zoloedov V.N., Zemskov A.M., Stupnitsky A.A. et al. Treatment of thymogen and myelopeptide in patients with bronchial asthma. Clinical medicine. 1995, No. 6, p. 43-45.

7. Smirnov B.C., Selivanov A.A. Bioregulators in the prevention and treatment of influenza. "Science", 1996, p.69.

8. Clinical applications of cytokines: the emerging markets. Ed. By R.M. Bonney, PJB Pablication LTD, 1996, p. 233.

9. Kaplanski G, Buscaylet S. et al. Antimony / interferon - gamma combination in the treatment of visceral leishmaniasis in AIDS. AIDS 1996. Vol.10, No. 10, p. 1172-1174.

10. Kraevsky A.A. The possibility of chemotherapy for AIDS. Vestnik RAN, 1999, No. 9, pp. 798-812.

11. Devyatkov N.D. and others. Features of biomedical application of millimeter waves. M., 1994, SU 818267.

12. Bessemeltsev S.S. et al. Effect of an in vitro variable magnetic field on immunocompetent blood cells and the clone-forming ability of bone marrow cells. Hematology and transphysiology, 1998, No. 2, pp. 12-15.

13. Tsib A.F. New and special technologies in medical radiology and radiation medicine. Vestnik RAMS, 1999, No. 10, M .: Medicine.

14. Abramovich S.G. et al. Features of the geroprotective effect of magnetotherapy in the elderly with combined cardiovascular pathology. Issues of balneology, physiotherapy and physiotherapy, 1999, No. 5, pp. 5-7.

15. RF patent No. 2053812, IPC A 61 N 5/00, 1996.

16. RF patent No. 2053811, IPC A 61 N 5/00, 1996.

17. RF patent No. 2134598, IPC A 61 N 5/00, 5/02, 1999.

18. RF patent No. 2216364, IPC A 61 N 2/04, 5/02, 2003.

19. RF patent No. 2183481, IPC A 61 N 2/04, 5/02, 2002.

20. Sinitsyn N.I., Elkin V.A. and others. The special role of the system "MM - waves - water environment" in nature. Biomedical Electronics, 1998, No. 1.

21. Petrosyan V.I. Zhiteneva E.A., Gulyaev Yu.V. et al. Physics of the interaction of MM waves with objects of various nature. Radio Engineering, 1996, No. 9. Magazine in the journal "Biomedical Electronics", No. 3.

22. Zmushko E.I., Belozerov E.S. HIV infection, a series of "Modern Medicine", 2000, St. Petersburg: "Peter", p.12-25, p.45-62.

23. Petrosyan V.I., Gulyaev Yu.V. etc. Interaction of physical and biological objects with electromagnetic radiation of the EHF band. Radio engineering and electronics, 1995, v.40, v.1.

24. Kaganov N.V. Spin dynamics in low concentrated paramagnets. Perm State University, 1998, Perm, pp. 2-15

25. Utility model of the Russian Federation No. 21522, IPC A 61 N 2/02, 5/02, 2002.

Table 1
The dynamics of the coefficient of the regenerating effect (α) Device name MP exposure days one 3 5 7 9 eleven 13 fifteen 17 19 21 Analysis / Session Number one 2 3 four 5 6 7 8 9 10 eleven Recovery factor value RP Absent MP Within the measurement error 0.10 0.20 0.30 0.45 0.70 0.80 0.90 In the limit of measurement error

table 2
The dynamics of the amount of virus in microsamples, copies / ml Name Exposure days one 3 5 7 9 eleven 13 fifteen 17 19 21 Analysis / Session Number one 2 3 four 5 6 7 8 9 10 eleven RP Is of constant importance MP 8700 8100 7200 6000 4600 2700 900 450 Virus concentration not determined

Table 3
Changes in the immune system in the subgroup A-1 (after burns) Indicators Before treatment (acute toxemia) During recovery Right after the course One month after the end of the course Lymphocytes, 10 ⁹ L 1.20 ± 0.12 1.88 ± 0.05 1.98 ± 0.07 SD2 ⁺ , 10 ⁹ / L 0.51 ± 0.06 0.99 ± 0.04 1.09 ± 0.05 SD4 ⁺ 10 ⁹ / L 0.21 ± 0.03 0.63 ± 0.02 0.69 ± 0.04 SD8 ⁺ 10 ⁹ / L 0.39 ± 0.02 0.42 ± 0.01 0.44 ± 0.02 CD4 ⁺ / CD8 ⁺ 0.63 ± 0.03 1.51 ± 0.05 1.65 ± 0.1 I _g M, g / l 0.91 ± 0.02 1.14 ± 0.3 1.16 ± 0.04 I _g G, g / l 10.1 ± 0.6 11.30 ± 0.4 11.50 ± 0.5 I _g A, g / l 1.01 ± 0.03 1.81 ± 0.08 1.90 ± 0.09 Phagocytic index 1.90 ± 0.13 3.9 ± 0.17 4.9 ± 0.51

Table 4
The state of the immune system in subgroups A-2 and A-3 (post-infectious and post-stress complications) Indicators Before treatment After treatment Immediately upon completion of the course One month after the course Hemoglobin, g / l 108 ± 6 139 ± 9 144 ± 12 ESR, mm / hour 27 ± 4 11 ± 3 5 ± 2 White blood cells, 10 ⁹ / L 9.9 ± 0.9 7.1 ± 0.5 5.5 ± 0.4 Lymphocytes, 10 ⁹ / L 1.7 ± 0.3 2.7 ± 0.3 2.9 ± 0.3 T-lymphocytes, 10 ⁹ / L 0.45 ± 0.09 0.7 ± 0.05 0.8 ± 0.1 B-lymphocytes, 10 ⁹ / L 0.40 ± 0.06 0.35 ± 0.04 0.3 ± 0.05 I _g M, g / l 2.7 ± 0.4 2.1 ± 0.2 1.2 ± 0.3 I _g G, g / l 18 ± 1.9 14 ± 1,2 11.0 ± 0.9 I _g , A, g / l 3.9 ± 0.6 2.7 ± 0.4 2.1 ± 0.3 Leukocyte Intoxication Index 3.1 ± 0.1 0.8 ± 0.05 0.6 ± 0.04

Table 5
The state of the immune system in subgroup A-4 (after the exposure to ionizing radiation and chemical xenobiotics) Indicators Before treatment After treatment Immediately upon completion of the course One month after the course White blood cells, 10% 2.9 ± 0.5 4.8 ± 0.3 5.45 ± 0.3 Lymphocytes, 10% 0.9 ± 0.4 1.89 ± 0.4 2.61 ± 0.4 SD3 ⁺ , 10 ⁹ / L 0.23 ± 0.05 0.9 ± 0.1 1.01 ± 0.1 SD4 ⁺ , 10 ⁹ / L 0.07 ± 0.01 0.45 ± 0.04 0.61 ± 0.03 SD8 ⁺ , 10 ⁹ / L 0.16 ± 0.04 0.40 ± 0.05 0.42 ± 0.04 SD19 ⁺ , 10 ⁹ / L 0.13 ± 0.03 0.36 ± 0.05 0.40 ± 0.1

Table 6
The dynamics of laboratory indicators of immunity in the subgroup A-5 (rheumatoid arthritis) Indicators Before treatment After treatment Immediately upon completion of the course 3 months after the course Hemoglobin, g / l 101 ± 3,5 124 ± 4 131 ± 3 Red blood cells, 10 ¹² / l 3.3 ± 0.5 4.1 ± 0.5 4.3 ± 0.4 ESR, mm / hour 42.4 ± 3.6 21 ± 3,5 11 ± 4 CD4 ⁺ / CD8 ⁺ 3.3 ± 0.3 2.0 ± 0.2 1.8 ± 0.2 I _g M, g / l 3.6 ± 1.2 2.1 ± 1.1 1.6 ± 1.0 I _g G, g / l 19.7 ± 4.6 15.3 ± 3 12.9 ± 2 I _g , A, g / l 3.8 ± 1.1 3.0 ± 1.0 2.7 ± 0.9

Claims (5)

1. A method of restoring a violated human immune status, including a magnetoelectromagnetic effect on the body, characterized in that they simultaneously affect the region of the pulmonary circulation and the reticular tissue of the flat bones of the patient’s sternum with non-thermal microwave radiation with resonant frequencies selected from the range of 2-40 GHz, with a power flux density of not more than 1 mW / cm ² and an alternating magnetic field with a frequency of 10-100 Hz, magnetic induction amplitude of up to 50 mT, for 90 min, comprising one session. 2. The method according to claim 1, characterized in that the full cycle of exposure consists of 8-11 outpatient non-invasive sessions, which are carried out sequentially every other day for 2-3 weeks until the violated human immune status is restored and / or the HIV viral load is reduced infections. 3. The method according to claim 1, characterized in that two sources of alternating magnetic field are fixed with an elastic tape on the side surfaces of the anterior sternum. 4. The method according to claim 1, characterized in that the source of resonant non-thermal microwave radiation is fixed over the area of the solar plexus. 5. The method according to claims 1 to 4, characterized in that with each subsequent session the location of the sources of non-thermal microwave radiation and an alternating magnetic field is changed clockwise.