RU2271041C2 - Method for simultaneous modeling of chronic pancreatitis and hepatitis - Google Patents

Abstract

FIELD: experimental medicine, in particular simultaneous modeling of chronic pancreatitis and hepatitis.

SUBSTANCE: claimed method includes application of mixture containing polychlorinated biphenyls in form of 5 % solution in olive oil. Said solution is administered into stomach in dose of 0.12 ml/100 g of animal mass 1 time per day every day for 12 days.

EFFECT: method for simultaneous reproducing of chronic pancreatitis and hepatitis in animals.

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Description

The present invention relates to medicine, namely to experimental pharmacology, and can be used for simultaneous modeling of chronic pancreatitis and hepatitis.

The problem solved by this invention is the creation of a simultaneous model of chronic pancreatitis and hepatitis.

The authors in the medical and patent literature did not find information about the simultaneous model of chronic pancreatitis and hepatitis, while it is known that when the pancreas is affected, the liver is also affected and, conversely, that is often observed in the clinic. Only methods for modeling chronic pancreatitis and hepatitis separately are known.

To simulate chronic pancreatitis, the method of surgical intervention is known, while trypsin / Pathological physiology is introduced into the pancreatic duct // edited by Ado A.D., Novitsky V.V. Tomsk: Publishing house of Tomsk University, 1994, p. 371 /.

For the modeling of chronic hepatitis, various chemicals were used: carbon tetrachloride / Nikolaev S.M. // Herbal medicines for damage to the hepatobiliary system. - Novosibirsk, 1992, p. 70 /; tetracycline / Baraclay A.I., Prokhorova A.M. // Antibiotics. - 1967. No. 8 - p. 708-711 /.

The technical result of the invention is the simultaneous modeling of chronic pancreatitis and hepatitis.

The method is illustrated by the following drawings:

Figure 1. Dystrophy and necrosis of hepatocytes with the formation of fat-protein detritus during intoxication with sovtol. Hematoxylin-eosin stain. Microphoto, vol. 10, approx. 40.

Figure 2. Fatty degeneration of the liver with intoxication with sovtol. Hematoxylin-eosin stain. Microphoto, vol. 10, approx. 40.

Figure 3. When intoxication with sovtol around a dense pancreatic islet, the acini increase and become oxyphilic, their serocytes are filled with secretory granules. The remaining end sections are densified and devoid of zymogenic and homogeneous zones. Hematoxylin-eosin stain. Microphoto, vol. 10, approx. 40.

Figure 4. Destruction of the secretory departments of the pancreas during intoxication with sovtol. End sections are reduced in size. Acinocytes are deprived of zymogenic and homogeneous zones, cell boundaries are not determined. Hematoxylin-eosin stain. Microphoto, vol. 10, approx. 40.

Figure 5. The endocrine and exocrine parts of the pancreas of the control group of animals (healthy). Hematoxylin-eosin stain. Microphoto, vol. 10, approx. 40.

6. Zone of the central vein of the liver of the control group of animals (healthy). Hematoxylin-eosin stain. Microphoto, vol. 10, approx. 40.

The proposed method is as follows:

Rats are injected with a 5% solution of Sovtol, which is a mixture of polychlorinated biphenyls / Tutelyan V.A. et al., 1983 / in olive oil at a dose of 0.12 ml per 100 g of animal mass once every day for 12 days, intragastrically using a probe. The optimal mode of administration was established experimentally.

After intoxication, a vivid picture of poisoning was observed: the animals had an unhealthy appearance, were passive, all lost from the original mass from 10 to 30%, out of 40 animals, 17 animals died, which is 42.5%.

After the experiment, the surviving rats were killed by decapitation under light ether anesthesia. For histological examination, the pancreas and liver were fixed in 10% formalin and embedded in paraffin. Sections 7 μm thick were stained with hematoxylin-eosin.

As our experiments showed, in experimental animals, after intoxication with sovtol, toxic necrosis and fatty hepatitis developed.

In the pancreas, chronic pancreatitis was observed, expressed in the form of destructive processes of individual or groups of acini, as well as the islet apparatus.

Example

A healthy rat weighing 250 g was injected with a 5% solution of Sovtol in olive oil at a dose of 0.12 ml per 100 g of animal body weight. Administration: once using a probe for 12 days daily. After intoxication, the rat was killed by decapitation under light ether anesthesia, the pancreas and liver were fixed in 10% formalin and embedded in paraffin. After conducted histological studies of these organs.

The method is illustrated by the following figures: intoxication - 1, 2, 3, 4; healthy - 5, 6.

Claims (1)

A method for simulating chronic pancreatitis and hepatitis at the same time, characterized in that a 5% solution of Sovtol, which is a mixture of polychlorinated biphenyls, in olive oil in a dose of 0.12 ml per 100 g of animal weight is administered intragastrically once daily for 12 days.

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