RU2223777C2 - Method for prophylaxis and treatment of experimental chronic pancreatitis and hepatitis - Google Patents

Abstract

FIELD: medicine, experimental pharmacology, phytotherapy. SUBSTANCE: for prophylaxis and treatment of indicated diseases method involves administration of species infusion in the concentration 1:10. Infusion comprises the following components: big burdock roots, swampy cudweed herb, marsh-mallow roots, glabrous licorice roots, common oak bark, medicinal sweet clove herb, common motherwort herb, knot-grass herb, purple coneflower herb, colt's foot leaves, walnut leaves taken in the ratio = 2:1:2:2:2:2:1:1:1:2: 1, respectively. Infusion is given in the dose 0.3 ml per 100 g of body mass of laboratory animal for 10 days. Invention is directed for the enhanced effectiveness in prophylaxis and treatment of chronic pancreatitis and hepatitis developing simultaneously. EFFECT: enhanced effectiveness of method. 16 dwg, 3 ex

Description

 The proposed group of inventions relates to medicine, namely to experimental and clinical pharmacology and can be used for the prevention and treatment of chronic pancreatitis and hepatitis in the experiment and for the simultaneous modeling of chronic pancreatitis and hepatitis.

 The problem solved by the group of inventions is to obtain a method for the prevention and treatment of chronic pancreatitis and hepatitis, which, as practice shows, often develop in parallel. To develop such a method, the second invention was carried out, aimed at creating a simultaneous model of chronic pancreatitis and hepatitis.

 Thus, the claimed group of inventions is associated with a single inventive concept.

 The authors in the medical and patent literature did not find information on the prevention and treatment of chronic pancreatitis and hepatitis at the same time, but some methods for treating these diseases separately were known and found, while no information was found on prevention.

 There is a method of treating chronic pancreatitis using the collection of medicinal plants: bitter wormwood grass, pharmacy chamomile flowers, perforated St. John's wort grass, swamp grass, peppermint leaf, elecampane roots, taken in a ratio of 1.5: 2: 2: 2: 1, 5: 2. / Nogaller A.M., Nikolaeva V.G., Alekseev V.F. // Herbal medicine for diseases of the digestive system. Ryazan, 1987, p. 86-89 /.

 A known method of treating toxic and viral hepatitis using hepatoprotector of plant origin - Silibora / Skakun P.P. et al. // Pharmacy. - 1987. - 6. - p. 13-17 /; collection "Hepafit" / Patent 2128515 from 04/10/99 /.

 The authors in the medical and patent literature did not find information about the simultaneous model of chronic pancreatitis and hepatitis, while it is known that when the pancreas is affected, the liver is also affected and, conversely, that is often observed in the clinic. Only methods for modeling chronic pancreatitis and hepatitis separately are known.

 To simulate chronic pancreatitis, the method of surgical intervention is known, while trypsin / Pathological physiology is introduced into the pancreatic duct // edited by Ado A.D., Novitsky V.V. Tomsk: Publishing house of Tomsk University, 1994, p. 371 /.

 For the modeling of chronic hepatitis, various chemicals were used: carbon tetrachloride / Nikolaev S.M. // Herbal medicines for damage to the hepatobiliary system. - Novosibirsk, 1992, p. 70 /; tetracycline / Baraclay A.I., Prokhorova A.M. // Antibiotics. - 1967.8 - p.708-711 /.

 The technical result is the prevention and treatment of simultaneous chronic pancreatitis and hepatitis.

The method is illustrated by the following figures:
Figure 1. An extensive zone of liver necrosis after intoxication with sovtol. Hematoxylin-eosin stain. Microphoto, vol. 10, approx. 40.

 FIG. 2. Destructive processes of the exocrine part of the pancreas after intoxication with sovtol. Hematoxylin-eosin stain. Microphoto, vol. 10, approx. 40.

 FIG. 3. The zone of the central vein of the liver zone of the control group of animals (healthy). Hematoxylin-eosin stain. Microphoto, vol. 10, approx. 40.

 FIG. 4. The endocrine and exocrine parts of the pancreas of the control group of animals (healthy). Hematoxylin-eosin stain. Microphoto, vol. 10, approx. 40.

 FIG. 5. Small droplet fat inclusions in hepatocytes during intoxication with sovtol and subsequent treatment with the proposed collection. Hematoxylin-eosin stain. Microphoto, vol. 10, approx. 40.

 FIG. 6. The exocrine part of the pancreas during intoxication with sovtol and subsequent treatment with the proposed collection. Hematoxylin-eosin stain. Microphoto, vol. 10, approx. 40.

 FIG. 7. Reduction of fat vacuoles in the central part of the liver lobule after intoxication with sovtol, when the proposed collection was introduced before intoxication for prophylactic purposes. Hematoxylin-eosin stain. Microphoto, vol. 10, approx. 40.

 FIG. 8. Weakly expressed destructive changes in the pancreas after intoxication with sovtol, when the proposed collection was introduced before intoxication for prophylactic purposes. Hematoxylin-eosin stain. Microphoto, vol. 10, approx. 90.

 Fig.9. Diffuse fatty degeneration in the zone of the liver triad during intoxication with sovtol and subsequent treatment with pancreatin. Hematoxylin-eosin stain. Microphoto, vol. 10, approx. 20.

 FIG. 10. Destructive processes of the exocrine part of the pancreas during intoxication with sovtol and subsequent treatment with pancreatin. Hematoxylin-eosin stain. Microphoto, vol. 10, approx. 40.

 The proposed method of prevention and treatment is as follows: rats are injected at a concentration of 1:10 infusion of the collection containing the roots of the marshmallow medicinal, licorice, burdock big, grass dried cinnamon, sweet clover, motherwort, birdwort, Echinacea purpurea, bark of ordinary oak, leaves of coltsfoot, walnut, taken in a ratio of 2: 2: 2: 1: 2: 1: 1: 1: 2: 2: 1. The infusion is administered intragastrically at a dose of 0.3 ml per 100 g of body weight for 10 days.

 The experiment involved 5 groups of 10 animals.

 The first group of rats was injected intragastrically with a collection dose of 0.3 ml per 100 g of body weight for 10 days as a prophylactic. The collection infusion was prepared at a concentration of 1:10 in accordance with the requirements of Global Fund XI.

 The second group was on a regular vivarium diet.

 After 10 days, both groups began to receive "Sovtol", consisting of a mixture of polychlorinated biphenyls and trichlorobenzene. Sovtol was diluted to 5% concentration in olive oil and was administered intragastrically at a dose of 0.12 ml per 100 g of animal body weight for 12 days.

 After intoxication, all rats were killed by decapitation under mild ether anesthesia, the pancreas and liver were removed, which were fixed in 10% formalin and embedded in paraffin for histological analysis.

 The method is illustrated in Fig.7 and 8.

 The remaining three groups of 10 rats each were administered a 5% solution of sovtol in olive oil at a dose of 0.12 ml per 100 g of animal weight for 12 days. During the experiment, the death of 17 animals from poisoning was observed (42.5%).

 After 12 days, the surviving animals of one group were killed by decapitation under mild ether anesthesia. Of the remaining animals, 2 groups of 5-6 rats were formed in each for subsequent treatment.

 The first group was treated with the introduction of pancreatin in a dose of 0.4 ml per 100 g of animal body weight.

 The second group was treated by introducing the proposed collection at a dose of 0.3 ml per 100 g of animal body weight.

 The treatment was continued for 10 days.

 No deaths were observed during treatment; after the treatment was completed, the animals were killed by decapitation under light ether anesthesia. The pancreas and liver were fixed in 10% formalin and embedded in paraffin for further histological examination.

 The method is illustrated by the following figures: intoxication sovtol - 1, 2; Pancreatin treatment (comparison group) - 9, 10; collection treatment - 5, 6; healthy - 3, 4.

The technical result of the invention is the simultaneous modeling of chronic pancreatitis and hepatitis. The method is illustrated by the following figures:
11. Dystrophy and necrosis of hepatocytes with the formation of fat-protein detritus during intoxication with sovtol. Hematoxylin-eosin stain. Microphoto, approx. 10, vol. 40.

 Fig. 12. Fatty degeneration of the liver with intoxication with sovtol. Hematoxylin-eosin stain. Microphoto, approx. 10, vol. 40.

 FIG. 13. During intoxication with sovtol around a dense pancreatic islet, the acini increase and become oxyphilic, their serocytes are filled with secretory granules. The remaining end sections are densified and devoid of zymogenic and homogeneous zones. Hematoxylin-eosin stain. Microphoto, approx. 10, vol. 40.

 FIG. 14. The destruction of the secretory departments of the pancreas during intoxication with sovtol. End sections are reduced in size. Acinocytes are deprived of zymogenic and homogeneous zones, cell boundaries are not determined. Hematoxylin-eosin stain. Microphoto, approx. 10, vol. 40.

 Fig.15. The endocrine and exocrine parts of the pancreas of the control group of animals (healthy). Hematoxylin-eosin stain. Microphoto, vol. 10, approx. 40.

 FIG. 16. The zone of the central vein of the liver of the control group of animals (healthy). Hematoxylin-eosin stain. Microphoto, vol. 10, approx. 40.

 The proposed method is as follows.

 Rats were injected with a 5% solution of Sovtol, which is a mixture of polychlorinated biphenyls and trichlorobenzene / Tutelyan V.A. et al., 1983 / in olive oil at a dose of 0.12 ml per 100 g of animal mass once, intragastrically with a probe for 12 days. The optimal mode of administration was established experimentally.

 After intoxication, a vivid picture of poisoning was observed: the animals had an unhealthy appearance, were passive, all lost from the original mass from 10 to 30%, out of 40 animals, 17 animals died, which is 42.5%.

 After the experiment, the surviving rats were killed by decapitation under light ether anesthesia. For histological examination, the pancreas and liver were fixed in 10% formalin and embedded in paraffin. Sections 7 μm thick were stained with hematoxylin-eosin.

 As our experiments showed, in experimental animals, after intoxication with sovtol, toxic necrosis and fatty hepatitis developed.

 In the pancreas, chronic pancreatitis was observed, expressed in the form of destructive processes of individual or groups of acini, as well as the islet apparatus.

 Example 1

 One rat weighing 250 g was injected as a prophylactic with a collection infusion at a concentration of 1: 10, prepared in accordance with the requirements of GF XI once a day for 10 days. After prophylaxis, a 5% solution of Sovtol in olive oil was administered at a dose of 0.12 ml per 100 g of animal body weight for 12 days. At the end of the term, the rat was killed by decapitation under light ether anesthesia, the pancreas and liver were fixed in 10% formalin and embedded in paraffin for histological examination.

 As can be seen from the proposed figures (7, 8), in comparison with the control (see example 3), in rats receiving prophylactic collection, a milder course was noted, the symptoms and severity of the diseases were less pronounced. The absence of deaths during intoxication was also noted.

 Example 2

 Two healthy rats were injected with a 5% solution of Sovtol in olive oil for 12 days. After one rat was killed by decapitation under mild ether anesthesia.

 The remaining rat was treated for 10 days with infusion collection. At the end of treatment, the rat was killed by decapitation under light ether anesthesia. The pancreas and liver of all rats were fixed in 10% formalin and embedded in paraffin for histological examination. The results in the following figures: treatment - 5, 6; intoxication - 1, 2; healthy - 3, 4.

 Example 3

 A healthy rat weighing 250 g was injected with a 5% solution of Sovtol in olive oil at a dose of 0.12 ml per 100 g of animal body weight. Administration: once with a probe for 12 days. After intoxication, the rat was killed by decapitation under mild ether anesthesia. The pancreas and liver were fixed in 10% formalin and embedded in paraffin. After conducted a histological examination of these organs.

 The method is illustrated by the following figures: intoxication - 11, 12, 13, 14; healthy - 15, 16.

Claims (1)

A method for the prevention and treatment of chronic pancreatitis and hepatitis in an experiment, characterized in that the infusion is collected at a concentration of 1:10, containing roots of burdock, grass of dried cinnamon, medicinal marshmallow roots, naked licorice, common oak bark, medicinal sweet clover grass, motherwort grass ordinary, grass of the mountaineer bird, grass of Echinacea purpurea, leaves of coltsfoot, leaves of walnut taken in a ratio of 2: 1: 2: 2: 2: 2: 1: 1: 1: 2: 1, while it is introduced at a dose of 0.3 ml per 100 g of body weight of a laboratory animal during of 10 days.

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