RU2264357C2 - Biopreparation for environment purification from oil and oil products - Google Patents

Abstract

FIELD: biotechnology, in particular environment (especially soil) purification from oil and oil products.

SUBSTANCE: claimed preparation contains (mass %): peat 25-40; wood sawing 10-30; cattle droppings 0.5-3.0; lignin 0.5-2.5; biodestructing microorganisms in effective amount, and balance: bird dung. As biodestructing microorganisms preparation contains bacterium strain Pseudomonas putida SP-203, and/or bacterium strain Rhodococcus rubber PS-02, and/or micromycete strain Trichoderma citrinoviride VS-89, and/or micromycete strain Metarrihisium anisoplae (Metschn.) Soros AF-03. Bioprepatarion of present invention makes it possible to utilize wastes of agriculture and plant raw material processing industry.

EFFECT: high effective method for controlling of oil contaminations.

2 tbl, 16 ex

Description

The invention relates to a means of combating pollution of environmental objects, in particular soil, with oil and oil products.

Known biological product for cleaning environmental objects from oil and oil products, containing microorganisms, biodestructors and additives that help maintain their viability and oil-oxidizing activity in the form of oil, sucrose and polyethylene glycol (Patent RU No. 2191752, class 02 F 3/34, publ. 10.27.2002).

However, this known biological product, when used to combat pollution of environmental objects with oil and oil products, provides for additional introduction of oil into the environment.

Known biological product for cleaning environmental objects from oil and oil products containing microorganisms, biodestructors and components of their nutrition (Patent RU No. 2081854, CL 02 F 3/34, publ. 06/20/1997).

However, this well-known preparation contains expensive non-natural components of the nutrition of microorganisms-biodestructors.

Known biological product for cleaning environmental objects from oil and oil products containing microorganisms, biodestructors, their power sources and sorbent (Patent RU No. 2191753, class C 02 F 3/34, publ. 10.27.2002).

This known biological product contains an expensive sorbent, which is wood pulp granules coated with layers of a sorbent substance, as well as expensive power sources in the form of thiourea and sucrose.

Known biological product for cleaning environmental objects from oil and oil products, containing microorganisms, biodestructors and an organic substrate in the form of a processing product of plant material, including moss, wood, peat (Patent RU No. 2193533, CL 02 F 3/34, publ. 27.11 .2002).

The disadvantage of this known drug is the use in it of a natural plant substrate, not in its native form, but after its processing, which requires the use of labor and energy.

The closest analogue of the claimed biological product is a known biological product for cleaning environmental objects from oil and oil products, containing microorganisms, biodestructors and peat (Patent RU No. 2122980, class 02 F 3/34, publ. 10.12.1998).

The well-known drug was used for soils with different pH, however, there is no data on its effectiveness for cleaning various types of soils. This well-known biological product in addition to the natural component of peat contains mineral additives as a source of nitrogen, phosphorus and potassium. This increases the cost of this drug and does not allow for its production to utilize agricultural waste and waste from vegetable processing industries. Such wastes accumulate in significant quantities and do not find ways to utilize them. In addition, this known preparation uses only one variant of the combination of strains of microorganisms-biodestructors, it cannot be used when other microorganisms-biodestructors are introduced into it.

The technical result achieved by the present invention is to expand its field of application with high efficiency for various types of soils, both soddy podzolic and black soil, increasing the availability of its components, saving the use of irreplaceable natural material - peat, ensuring the disposal of agricultural waste and processing industries plant materials, the versatility of the composition of the biological product, ensuring the possibility of using a spectrum of microorganisms in it Ktorov while ensuring the high efficiency of a biological product.

The specified technical result is achieved in that the biological product for cleaning the soil from oil and oil products containing microorganisms, biodestructors and peat, has the distinctive feature that it additionally contains sawdust, cattle manure and lignin in the following ratio, wt.%:

Peat 25-40 Sawdust 10-30 Cattle manure 0.5-3.0 Lignin 0.5-2.5 Biodestructive microorganisms in effective amount Bird droppings Rest

As a microorganism-biodestructor, it may contain a bacterial strain Pseudomonas putida SP-203.

It may also contain the bacterial strain Rhodococcus ruber PS-02 as a biodestructive microorganism.

Biodestructor microorganism can also be strains of bacteria Pseudomonas putida SP-203 and Rhodococcus ruber PS-02.

The biological product as a microorganism-biodestructor may also contain a strain of micromycete Trichoderma citrinoviride (teleomorph Hypocrea schweinitzii (Fresen) Sacc.) VS-89.

It may also contain a strain of micromycete Metarrihizium anisopliae (Metschn.) Soroc AF-03 as a biodestructive microorganism.

Strains of micromycetes Trichoderma citrinoviride (teleomorph Hypocrea schweinitzii (Fresen) Sacc.) VS-89 and Metarrihizium anisopliae (Metschn.) Soroc AF-03 can also be included in the composition of the biological product.

It may include the bacterial strain Pseudomonas putida SP-203 and the micromycete strain Trichoderma citrinoviride (teleomorph Hypocrea schweinitzii (Fresen) Sacc.) VS-89 as biodestructive microorganisms.

They can also be a strain of bacteria Rhodococcus ruber PS-02 and a strain of micromycete Trichoderma citrinoviride (teleomorph Hypocrea schweinitzii (Fresen) Sacc.) VS-89.

As biological microorganisms, the biological product may include the bacterial strain Pseudomonas putida SP-203 and the micromycete strain Metarrihizium anisopliae (Metschn.) Soroc AF-03.

The biological product may also contain a strain of bacteria Rhodococcus ruber PS-02 and a strain of micromycete Metarrihizium anisopliae (Metschn.) Soroc AF-03 as biodestructive microorganisms.

It may also contain bacterial strains of Pseudomonas putida SP-203 and Rhodococcus ruber PS-02 and the strain of micromycete Trichoderma citrinoviride (teleomorph Hypocrea schweinitzii (Fresen) Sacc.) VS-89 as microorganisms-biodestructors.

The biological product may also contain strains of bacteria Pseudomonas putida SP-203 and Rhodococcus ruber PS-02 and a strain of micromycete Metarrihizium anisopliae (Metschn.) Soroc AF-03 as biodestructive microorganisms.

As a biological microorganism, the biological product may also contain strains of micromycetes Trichoderma citrinoviride (teleomorph Hypocrea schweinitzii (Fresen) Sacc.) VS-89 and Metarrihizium anisopliae (Metschn.) Soroc AF-03 biodestructor and bacterium Pomida putida.

It may also contain, as biodestructive microorganisms, strains of micromycetes Trichoderma citrinoviride (teleomorph Hypocrea schweinitzii (Fresen) Sacc.) VS-89 and Metarrihizium anisopliae (Metschn.) Soroc AF-03 bacterial strain Rhodococcus ruber PS-02.

As microorganisms, biodestructors, the biological product can also contain strains of micromycetes Trichoderma citrinoviride (teleomorph Hypocrea schweinitzii (Fresen) Sacc.) VS-89 and Metarrihizium anisopliae (Metschn.) Soroc AF-03 biodestructor and 20 strains of bacterium Prucodis rucida Pruce -02.

It is advisable that the biological product contains strains of microorganisms-biodestructors in a final concentration of each (0.5-3) · 10 ⁸ cells / cm ³ preparation.

The strains of microorganisms-biodestructors used in the biological product are stored in the collection of the Department of Microbiology of the Moscow Agricultural Academy named after K.A. Timiryazev.

The following are examples of the implementation of the invention.

Example 1. Prepared a biological product with the following ratio of components, wt.%:

Peat 25 Sawdust 10 Cattle manure 0.5 Lignin 0.5 Microorganism-biodestructor: cells of the bacterial strain Pseudomonas putida SP-203 (cells / cm ³ preparation) 3 · 10 ⁸ Bird droppings Rest

Example 2. Prepared a biological product with the following ratio of components, wt.%:

Peat 40 Sawdust thirty Cattle manure 3.0 Lignin 2,5 Microorganism-biodestructor: cells of the strain Rhodococcus ruber PS-02 (cells / cm ³ preparation) 0.510 ⁸ Bird droppings Rest

Example 3. Prepared a biological product with the following ratio of components, wt.%:

Peat 25 Sawdust 10 Cattle manure 0.5 Lignin 0.5 Biodestructive microorganisms: cells of the bacterial strain Pseudomonas putida SP-203 (cells / cm ³ preparation) 3 · 10 ⁸

cells of the bacterial strain Rhodococcus ruber PS-02

(cells / cm ³ preparation) 1 · 10 ⁸ Bird droppings Rest

Example 4. Prepared a biological product with the following ratio of components, wt.%:

Peat 25 Sawdust 10 Cattle manure 0.5 Lignin 0.5 Microorganism-biodestructor: spores of the strain of micromycete Trichoderma citrinoviride (teleomorph Hypocrea schweinitzii (Fresen) Sacc.) VS-89 (cells / cm ³ preparation) 2 · 10 ⁸ Bird droppings Rest

Example 5. Prepared a biological product with the following ratio of components, wt.%:

Peat 40 Sawdust twenty Cattle manure 2,5 Lignin 1,5 Microorganism-biodestructor: spores of the micromycete strain Metarrihizium anisopliae (Metschn.) Soroc AF-02 (cells / cm ³ preparation) 2 · 10 ⁸ Bird droppings Rest

Example 6. Prepared a biological product with the following ratio of components, wt.%:

Peat thirty Sawdust 10 Cattle manure 2,5 Lignin 1,5 Biodestructive microorganisms: spores of the strain of micromycete Trichoderma citrinoviride (teleomorph Hypocrea schweinitzii (Fresen) Sacc.) VS-89 (cells / cm ³ preparation) 2 · 10 ⁸ spores of the micromycete strain Metarrihizium anisopliae (Metschn.) Soroc AF-03 (cells / cm ³ preparation) 2 · 10 ⁸ Bird droppings Rest

Example 7. Prepared a biological product with the following ratio of components, wt.%:

Peat thirty Sawdust twenty Cattle manure 1,5 Lignin 0.5 Biodestructive microorganisms: cells of the bacterial strain Pseudomonas putida SP-203 (cells / cm ³ preparation) 1 · 10 ⁸ spores of the strain of micromycete Trichoderma citrinoviride (teleomorph Hypocrea schweinitzii (Fresen) Sacc.) VS-89 (cells / cm ³ preparation) 2 · 10 ⁸ Bird droppings Rest

Example 8. Prepared a biological product with the following ratio of components, wt.%:

Peat 40 Sawdust 10 Cattle manure 3.0 Lignin 0.5 Biodestructive microorganisms: cells of the strain of bacteria Rhodococcusruber PS-02 (cells / cm ³ preparation) 1 · 10 ⁸ spores of the strain of micromycete Trichoderma citrinoviride (teleomorph Hypocrea schweinitzii (Fresen) Sacc.) VS-89 (cells / cm ³ preparation) 2 · 10 ⁸ Bird droppings Rest

Example 9. Prepared a biological product with the following ratio of components, wt.%:

Peat 40 Sawdust 10 Cattle manure 3.0 Lignin 0.5 Biodestructive microorganisms: cells of the bacterial strain Pseudomonas putida SP-203 (cells / cm ³ preparation) 1 · 10 ⁸ spores of the micromycete strain Metarrihizium anisopliae (Metschn.) Soroc AF-03 (cells / cm ³ preparation) 2 · 10 ⁸ Bird droppings Rest

Example 10. Prepared a biological product with the following ratio

components, wt.%:

Peat 40 Sawdust 10 Cattle manure 3.0 Lignin 0.5 Biodestructive microorganisms: cells of the bacterial strain Rhodococcus ruber PS-02 (cells / cm ³ preparation) 1 · 10 ⁸ spores of the micromycete strain Metarrihizium anisopliae (Metschn.) Soroc AF-03 (cells / cm ³ preparation) 2 · 10 ⁸ Bird droppings Rest

Example 11. Prepared a biological product with the following ratio of components, wt.%:

Peat 40 Sawdust twenty Cattle manure 2.0 Lignin 1,0 Biodestructive microorganisms: cells of the bacterial strain Pseudomonas putida SP-203 (cells / cm ³ preparation) 1.5 · 10 ⁸ cells of the strain of bacteria Rhodococcus ruber PS-02 (cells / cm ³ preparation) 1 · 10 ⁸ spores of the strain of micromycete Trichoderma citrinoviride (teleomorph Hypocrea schweinitzii (Fresen) Sacc.) VS-89 (cells / cm ³ preparation) 2 · 10 ⁸ Bird droppings Rest

Example 12. Prepared a biological product with the following ratio of components, wt.%:

Peat 25 Sawdust twenty Cattle manure 2.0 Lignin 1,0 Biodestructive microorganisms: cells of the bacterial strain Pseudomonas putida SP-203 (cells / cm ³ preparation) 1.5 · 10 ⁸ cells of the strain of bacteria Rhodococcus ruber PS-02 (cells / cm ³ preparation) 1 · 10 ⁸ spores of the micromycete strain Metarrihizium anisopliae (Metschn.) Soroc AF-03 (cells / cm ³ preparation) 2 · 10 ⁸ Bird droppings Rest

Example 13. Prepared a biological product with the following ratio of components, wt.%:

Peat 25 Sawdust thirty Cattle manure 2,5 Lignin 0.5 Biodestructive microorganisms: spores of the strain of micromycete Trichoderma citrinoviride (teleomorph Hypocrea schweinitzii (Fresen) Sacc.) VS-89 (cells / cm ³ preparation) 2 · 10 ⁸ spores of the micromycete strain Metarrihizium anisopliae (Metschn.) Soroc AF-03 (cells / cm ³ preparation) 2 · 10 ⁸ cells of the bacterial strain Pseudomonas putida SP-203 (cells / cm ³ preparation) 1.5 · 10 ⁸ Bird droppings Rest

Example 14. Prepared a biological product with the following ratio of components, wt.%:

Peat 25 Sawdust thirty Cattle manure 2,5 Lignin 0.5 Biodestructive microorganisms: spores of the strain of micromycete Trichoderma citrinoviride (teleomorph Hypocrea schweinitzii (Fresen) Sacc.) VS-89 (cells / cm ³ preparation) 2 · 10 ⁸ spores of the micromycete strain Metarrihizium anisopliae (Metschn.) Soroc AF-03 (cells / cm ³ preparation) 2 · 10 ⁸ cells of the bacterial strain Rhodococcus cells ruber PS-02 (cells / cm ³ preparation) 1 · 10 ⁸ Bird droppings Rest

Example 15. Prepared a biological product with the following ratio of components, wt.%:

Peat 25 Sawdust thirty Cattle manure 0.5 Lignin 1,5 Biodestructive microorganisms: cells of the bacterial strain Pseudomonas putida SP-203 (cells / cm ³ preparation) 1.5 · 10 ⁸ cells of the bacterial strain Rhodococcus cells ruber PS-02 (cells / cm ³ preparation) 1 · 10 ⁸ spores of the strain of micromycete Trichoderma citrinoviride (teleomorph Hypocrea schweinitzii (Fresen) Sacc.) VS-89 (cells / cm ³ preparation) 2 · 10 ⁸ spores of the micromycete strain Metarrihizium anisopliae (Metschn.) Soroc AF-03 (cells / cm ³ preparation) 2 · 10 ⁸ Bird droppings Rest

Example 16. On the example of artificially oil-contaminated samples of sod-podzolic and chernozem soil, biological preparations were tested according to examples 1-15. At the same time, oil was introduced into soil samples in an amount of 2.5% of their weight. For artificial contamination of soil samples, two types of oil were used:

1. Oil of the Kharyachinsky field (oil A): medium paraffin with a paraffin content of 2.4%, sulfur with a content of 0.81% and with a pour point of + 10 ° C;

2. Oil of the Sugmutskoye field (oil B): highly paraffin with a paraffin content of 7.9%, sulfur with a content of 0.91% and a pour point of + 29 ° C.

After biologics were added to samples of soil contaminated with oil, we monitored the process of reducing the oil content in them after 2 months, after 4 months, and after 6 months.

The control was the same soil samples without the introduction of biological products.

Table 1, 2 shows the average results of observations and indicate the high efficiency of all tested biological products according to examples 1-15. Observations showed that the process of natural cleaning of the soil from oil pollution is much less intense than in the presence of biological products according to the invention. So, in samples of sod-podzolic soil (see Table 2), in which no biological products were added, the average oil content for 6 months decreased by 58.2%, and the oil content B - by 51.2%. In the presence of biological products for the same period of time, the content of oil A and oil B decreased on average by 73.0% and 70.1%, respectively. In samples of chernozemic soil (see Table 2), without biologics being added to them for 6 months, the content of oil A decreased on average by 57.4%, and the content of oil B - by 53.6%. Over the same period of time in the presence of biological products, the oil content A decreased by 82.0%, and the oil content B - by 79.7%. The data indicate the high effectiveness of biological products, which are included in the various declared variants of microorganisms-biodestructors.

Thus, the biological product according to the invention is highly effective in its use for combating environmental pollution by oil and oil products. It includes waste from agriculture and plant processing industries, which allows for their disposal and prevent their accumulation in farms and factories, ensures the availability and uninterrupted supply of raw materials for the preparation of a biological product. Reduced use of expensive non-renewable natural material - peat. The optimally selected qualitative and quantitative composition of the components of the biological product makes it universal and allows you to enter into it a wide range of cultures of microorganisms, biodestructors of oil and oil products.

Table 1
The dynamics of changes in oil content in contaminated sod-podzolic soil No. Oil Biological product The decrease in oil content,% After 2 months After 4 months After 6 months 1 Kharyanskoye field, medium paraffin - 28.5 49.5 58.2 2 Kharyanskoye field, medium paraffin + 48.5 68.5 73.0 3 Sugmutskoye field, highly paraffinous - 18.6 38.7 51,2

4 Sugmutskoye field, highly paraffinous + 41.0 61.0 70.1

table 2
Dynamics of changes in oil content in contaminated chernozem soil No. Oil Biological product The decrease in oil content,% Across
2 months Across
4 months After 6 months 1 Kharyanskoye field, medium paraffin - 31.8 51.8 57.4 2 Kharyanskoye field, medium paraffin + 41.2 71.2 82.0 3 Sugmutskoye field, highly paraffinous - 24.2 44,2 53.6 4 Sugmutskoye field, highly paraffinous + 40.5 70.5 79.7

Claims (2)

1. A biological product for cleaning the soil from oil and oil products containing microorganisms, biodestructors and peat, characterized in that as microorganisms biodestructors it contains a bacterial strain Pseudomonas putida SP-203, and / or a bacterial strain Rhodococcus ruber PS-02, and / or a strain of micromycete Trichoderma citrinoviride (teleomorph Hypocrea schweinitzii (Fresen) Sacc.) VS-89, and / or a strain of micromycete Metarrihizium anisopliae (Metschn.) Soroc AF-03, as well as a biological product additionally contains sawdust, cattle manure the following content of components, wt.%: Peat 25-40 Sawdust 10-30 Cattle manure 0.5-3.0 Lignin 0.5-2.5 Biodeostructural microorganisms in effective amount Bird droppings Rest 2. The biological product according to claim 1, characterized in that it contains strains of microorganisms-biodestructors in a final concentration of each (0.5-3) · 10 ⁸ cells / cm ³ preparation.