RU2250265C2 - Method for production of feedstuff - Google Patents

Abstract

FIELD: biotechnology, in particular feedstuff production.

SUBSTANCE: claimed method includes cultivation of lactic and propionic acid bacteria pairwise: Lactobacillus plantarum B 578/25 and Propionibacterium freudenreichii subsp. Shermanii Ac102/12 or Lactobacillus plantarum B 578/25 and Propionibacterium acnes Ac1450/28 in post-alcohol distillery slop. Obtained biomass is divided onto solid and liquid phases. Solid phase is used as feedstuff product, and liquid one - as technological recycled water. Method of present invention makes in possible to utilize waste from alcohol production (i.e. post-alcohol distillery slop) and decrease nutrient consumption for alcohol yeast due to filtrate recycling into alcohol production.

EFFECT: environment friendly method; protein-vitamin feedstuff of high quality.

2 cl, 7 ex, 7 tbl

Description

The invention relates to the production of feed from waste products of the alcohol industry.

In the production of ethyl alcohol from grain raw materials, after distillation of alcohol from the mash, the waste of alcohol production remains - post-alcohol distillery stillage. It is a complex polydisperse system, the dry substances of which are in the form of suspensions and in a dissolved state.

Significant amounts of the stillage formed (13 decalitres per 1 decalitre of alcohol) and the absence of an effective and reliable method for its disposal make the task of processing post-alcohol distinction stillage.

This is because barda has a low solids content of 5-7%, can be stored for no more than a day, and its colloidal properties do not allow to provide the necessary quality of its separation into solid and liquid phases.

A known method for the production of fodder product, including the cultivation of fodder yeast on post-alcohol stillage with obtaining fodder product in the form of yeast biomass (USSR author's certificate No. 419552, C 12 D 13/06, publ. 15.03.74) [1].

However, protein feed in the form of biomass of fodder yeast obtained by this known method has insufficiently high feed value due to the need for mandatory heat treatment of biomass of fodder yeast, this is accompanied by significant losses of biologically active substances and requires high energy consumption.

A known method of producing a feed product, including the preparation of wort, fermenting it to obtain mash, distillation of the mash to produce ethanol and post-alcohol distillery stillage, which is used to prepare the feed product (patent RU No. 2127760, class C 12 P 7/06, publ. 20.03.99 ) [2].

The feed product according to this known method is obtained by mixing post-alcohol distillery stillage with husk grain used for making wort.

The method provides for the reception of the return of part of the post-alcohol distillery stillage to the stage of batch preparation, as a result of which the concentration of the wort prepared from it increases, which positively affects the process of fermentation.

However, the post-alcohol distillation stillage does not effectively affect the fermentation of the wort. She also has physico-chemical characteristics that do not allow her to return in significant quantities. The feed obtained by this known method has a low protein content.

A known method of producing a feed product, including making wort, fermenting it to obtain mash, distilling the mash to produce ethanol and post-alcohol stillage, cultivating microorganisms in the post-alcohol stillage to obtain their biomass, separating biomass into solid and liquid fractions, using the solid fraction as a feed product, and liquid - as circulating process water (USSR author's certificate No. 1500671, С 12 Р 7/06, publ. 08.04.87) [3] - prototype.

However, the feed obtained by this known method is subjected to mandatory heat treatment to terminate the activity of feed yeast, the biomass of which is obtained from the disposal of post-alcohol stillage. This requires increased energy consumption and is accompanied by a partial destruction of biologically active components of the feed. The production of fodder yeast is an aerobic process that requires high air consumption (up to 60 cubic meters per hour per 1 cubic meter of culture fluid). The liquid fraction returned to the previous stages of alcohol production is depleted in nutrients and therefore does not significantly affect the process.

The technical result achieved by the present invention is to improve the quality of the feed product, to ensure the complete utilization of the post-alcohol stillage stillage, to intensify the process of preparing the feed product by intensifying the process of fermentation of the wort, preventing acidification and reducing labor costs.

The specified technical result is achieved by the fact that in the method of producing a feed product, including making wort, fermenting it to obtain mash, distilling the mash to produce ethanol and post-alcohol stillage, cultivating microorganisms on the post-alcohol stillage to obtain their biomass, separating the biomass into solid and liquid fractions and the use of the solid fraction as a feed product, a distinctive feature is that pairs of lactic acid and propionic strains are grown on the post-alcohol bard mated bacteria: Lactobacillus plantarum in 578/25 and Propionibacterium freudenreichii subsp. shermanii Ac103 / 12; or Lactobacillus plantarum B578 / 25 and Propionibacterium acnes Ac1450 / 28.

The liquid biomass fraction is recommended to be used as recycled process water in the preparation of wort.

Lactic acid and propionic acid bacteria are anaerobes and therefore, when grown on the post-alcohol bard, aeration is not required, which reduces energy costs. When growing on a post-alcohol bard, lactic and propionic acid bacteria consume colloidal, soluble bard substances to obtain a culture fluid with physicochemical parameters that provide good filtering characteristics. This makes it possible to dehydrate the resulting biomass by 90% mechanically, which reduces energy consumption. The resulting protein-vitamin product contains 35-50% of a complete protein, is enriched by 30-40% with amino acids, vitamins, microelements, and the depleted filtrate of the treated stillage returns completely to the alcohol industry.

The filtrate of the treated stillage contains biologically active waste products of lactic and propionic acid bacteria, which serve as food sources for yeast fermenting wort in the production of alcohol.

When grown on a post-alcohol bard, lactic and propionic acid bacteria consume a significant part of the nutrient components, including those in colloidal and soluble form. Therefore, after the end of the fermentation process, the culture fluid has a minimum viscosity and is well separated into solid and liquid phases. Little dissolved substances remain in the liquid phase (1-1.7%), and it completely returns to the alcohol industry instead of water, for example, to the kneading stage in the preparation of wort.

Repeated repeated returns of the treated distillery stillage filtrate to a batch do not cause an increase in viscosity, an increase in the osmotic pressure of the fermented medium, accumulation of non-fermentable substances and other adverse effects in the alcohol industry, in each cycle, the bard is subjected to treatment with lactic and propionic acid bacteria and is depleted to 1-1.7% d.v. versus 3-5% in the stillage filtrate without treatment. The small amount of solids remaining in the filtrate, including nitrogenous, trace elements, vitamins, is used for growth and vital activity of alcoholic yeast, and sodium or calcium lactates and propionates play the role of stimulants and preservatives, protecting the wort from acidification.

Strain Lactobacillus plantarum B578 / 25. The strain was identified in accordance with Bergey's Manual of Determinative Bacteriology, 9th edition, 1994, Williams & Wilkins, USA, obtained from Lactobacillus plantarum BKM B-578 by step selection on media with high concentrations of lactic acid.

Cultural and morphological features of the strain: fixed rods of size (0.9-1.2) × (3-8) microns. Gram-positive. Non-spore forming. Aerial Tolerance. Fermentation of fructose, lactose, galactose, glucose (acid), maltose, mannitol, ribose, sucrose, cellobiose. Forms DL-forms of lactic acid. The optimum pH is 5.8-6.0. The optimum temperature is 37 ° C.

The method, conditions and composition of the media for long-term storage of the strain is lyophilization in separated milk under conditions deprived of oxygen.

The method, conditions and composition of the medium for the propagation of the strain is distilled water - 600 ml, meat water - 400 ml, yeast extract - 5.0 g, sodium acetate - 5.0 g, glucose - 2.5 g, ammonium citrate - 2, 0 g, K ₂ NRA ₄ - 2.0 g, MgSO ₄ · 7H ₂ O - 0.2 g, MgO ₄ · 4H ₂ O - 0.05 g, Tween 80 - 1 ml, agar - 5.0 g.

The strain is not zoopathogenic, phytopathogenic and is not dangerous for other reasons.

The strain is received and stored at the All-Russian Research Institute of Food Biotechnology (GNU VNIIIPBT). Address: 111033, Moscow, st. Scooter, d.4B. Tel 362-44-18.

Deposited as a strain of Lactobacillus plantarum B578 / 25. Depositor: Federal State Institution All-Russian State Research Institute for Control, Standardization and Certification of Veterinary Medicines - Center for Quality of Veterinary Medicines and Feed (FGU VGNKI) (Ministry of Agriculture of the Russian Federation, Department of Veterinary).

Strain Propionibacterium freudenreichii subsp. shermanii Ac103 / 12. The strain is identified in accordance with Bergey's Manual of Determinative Bacteriology, 9 edition, 1994, Williams & Wilkins, USA, obtained from Propionibacterium freudenreichii subsp. shermanii BKM-B103 (BKM Ac-2260) by step selection on media with high concentrations of propionic acid.

Cultural and morphological features of the strain: fixed rods of 0.5-1.5 microns in size, sometimes forming short curved chains, sometimes look like cocci. Gram-positive. Non-spore forming. Aerial Tolerance. Ferments fructose, lactose, galactose, glucose, mannose. The main product of metabolism is propionic acid. In the cell wall, the main component is meso-DAN. The optimum pH is 7.0-7.2. The optimum temperature is 32 ° C.

The method, conditions and composition of media for long-term storage of the strain is lyophilization in separated milk under conditions devoid of oxygen and 10% sugar gelatin agar.

The method, conditions and composition of the medium for the propagation of the strain is distilled water - 1000 ml, yeast extract - 10.0 g, K ₂ NRA ₄ - 1.0, Na ₂ HPO ₄ · 2H ₂ O - 3.0 g, Na lactate ( 70%) - 40 ml. Dissolve all ingredients and add sodium lactate. The optimum pH is 7.0.

The strain is not zoopathogenic, phytopathogenic and is not dangerous for other reasons. The strain is received and stored at the All-Russian Research Institute of Food Biotechnology (GNU VNIIIPBT). Address: 111033, Moscow, st. Scooter, d.4B. Tel 362-44-18.

Deposited as a strain of Propionibacterium freudenreichii subsp. shermanii Ac103 / 12.

Depositor: Federal State Institution All-Russian State Research Institute for Control, Standardization and Certification of Veterinary Medicines - Center for Quality of Veterinary Medicines and Feed (FGU VGNKI) (Ministry of Agriculture of the Russian Federation, Department of Veterinary).

Strain Propionibacterium acnes Ac1450 / 28. The strain was identified in accordance with Bergey's Manual of Determinative Bacteriology, 9th edition, 1994, Williams & Wilkins, USA, obtained from Propionibacterium acnes BKM Ac-1450 by stepwise selection on media with high concentrations of propionic acid.

Cultural and morphological features of the strain: fixed rods of 0.5-1.5 microns in size, slightly curved, at a young age can be long, branching. In older cultures, cells often have a coccoid shape. Gram-positive. Non-spore forming. Aerial Tolerance. Catalopositive. Ferments fructose, sucrose, maltose, galactose, glucose, mannose. The main product of metabolism is propionic, traces of acetic, succinic, lactic and formic acids. The optimum pH is 7.0. The optimum temperature is 37 ° C.

The method, conditions and composition of the media for long-term storage of the strain is lyophilization in separated milk under conditions devoid of oxygen, 10% sugar gelatin agar and in a semi-liquid medium under oil.

The method, conditions and composition of the medium for the propagation of the strain - distilled water - 1000 ml, yeast extract - 10.0 g, K ₂ NRA ₄ - 1.0 g, Na ₂ HPO ₄ · 2H ₂ O - 3.0 g, lactate Na (70%) - 40 ml. Dissolve all ingredients and add sodium lactate. The optimum pH is 7.0.

The strain is not zoopathogenic, phytopathogenic and is not dangerous for other reasons. The strain is received and stored at the All-Russian Research Institute of Food Biotechnology (GNU VNIIIPBT). Address: 111033, Moscow, st. Scooter, d.4B. Tel 362-44-18.

Deposited as a strain of Propionibacterium acnes Ac1450 / 28.

Depositor: Federal State Institution All-Russian State Research Institute for Control, Standardization and Certification of Veterinary Medicines - Center for Quality of Veterinary Medicines and Feed (FGU VGNKI) (Ministry of Agriculture of the Russian Federation, Department of Veterinary).

The following are examples of the implementation of the invention.

Example 1

The strain Lactobacillus plantarum B578 / 25 was obtained by repeated (at least 30) reseeding of cells of the original strain of Lactobacillus plantarum B 578 on a solid nutrient medium of the following composition:

Distilled water 600 ml

Meat water 400 ml

Yeast Extract 5.0 g

Sodium acetate 5.0 g

Glucose 2.5 g

Ammonium Citrate 2.5 g

K ₂ NRA ₄ 2.0 g

MgSO ₄ · 7H ₂ O 0.2 g

MnO ₄ · 4H ₂ O 0.05 g

Twin 80 1 ml

Agar 20.0 g

The content of free lactic acid in the medium was gradually increased from 0 to 1.5%. Cells were incubated in solid nutrient medium at 37 ° C for 24 hours. The selection was carried out according to the number of colonies and acid-forming ability, which was determined by titration. Those colonies were selected whose growth on solid nutrient medium was not inhibited in the presence of 1.5% lactic acid. After 30 passages, the strain reached genetic resistance. The selection results of a new strain of Lactobacillus plantarum B 578/25 by stepwise selection are shown in Table 1, which indicates that when the content of lactic acid in the medium is up to 2.0%, the cell growth of the new strain of lactic acid bacteria (unlike the original strain) is not inhibited and is characterized by a sufficiently large number of cells. With a higher content of lactic acid (2.5%), a sharp decrease in the number of cells occurs and the morphology of the cells changes.

Table 1
The effect of the concentration of lactic acid on the growth of the strain of Lactobacillus plantarum B 578/25 The name of the strain The concentration of lactic acid,% The number of cells in 1 ml of culture fluid Lactobacillus plantarum 0 2.010 ⁹ B 578/25 0.5 1.510 ⁹ 1,0 1,0 · 10 ⁷ 1.25 1.5 · 10 ⁶ 1,5 1,0 · 10 ⁵ 2.0 1,0 · 10 ⁴ 2,5 0.5 · 10 ²

To propagate the strain, the selected largest colonies were subcultured onto a liquid nutrient medium of the following composition:

Distilled water 600 ml

Meat water 400 ml

Yeast Extract 5.0 g

Sodium acetate 5.0 g

Glucose 2.5 g

Ammonium Citrate 2.5 g

K ₂ NRA ₄ 2.0 g

MgSO ₄ · 7H ₂ O 0.2 g

MnO ₄ · 4H ₂ O 0.05 g

Twin 80 1 ml

Agar 5.0 g

Example 2

The strain Lactobacillus plantarum B 578/25 was evaluated by its ability to form an increased protein content by culturing it on a liquid nutrient medium of the composition specified in example 1, by measuring the amount of biomass and crude protein formed in the culture fluid.

An inoculum in an amount of five volume percent was introduced into flasks with a 750 ml liquid nutrient medium and cultivation was carried out under stationary conditions, subject to the following parameters: temperature 37 ° C, pH 6.0. A 20% sodium hydroxide solution was used as a neutralizing agent. After 24 hours of cultivation, bacterial growth and the amount of biomass and crude protein formed in the biosynthesis in the culture fluid were determined.

The results are presented in table 2.

table 2
Characterization of the strain Lactobacillus plantarum B 578/25 Name of indicator Indicator value The number of cells in 1 ml of culture fluid 0.810 ⁹ The specific yield of the substrate, wt. % 95.0 Mass fraction of crude protein,% 49

Based on the results obtained, it was concluded that the selected strain has high productivity with respect to biomass accumulation (the initial content of bacterial cells was 1.0 · 10 ² ), a high specific yield from the substrate used and the accumulation of crude protein up to 49% versus 25% in the original strain.

Example 3

Strain Propionibacterium freudenreichii subsp. shermanii Ac103 / 12 was obtained by repeated (at least 30) reseeding of cells of the original strain Propionibacterium freudenreichii subsp. shermanii Ac-103 on a solid nutrient medium of the following composition:

Distilled water 1000 ml

Yeast extract 10.0 g

KH ₂ PO ₄ 1.0 g

Na ₂ HPO ₄ · 2H ₂ About 3.0 g

Sodium Lactate (70% solution) 40.0 ml

CoSO ₄ 1.0 mg

Agar 20.0 g

The content of free propionic acid in the medium was gradually increased from 0 to 1.5%. Cells were incubated in solid nutrient medium at 37 ° C for 24 hours. The selection was carried out according to the number of colonies and acid-forming ability, which was determined by titration. Those colonies were selected whose growth on a solid nutrient medium was not inhibited in the presence of 1.5% propionic acid. After 30 passages, the strain reached genetic resistance. The results of the selection of a new strain of Propionibacterium freudenreichii subsp. shermanii Ac103 / 12 by step selection is shown in Table 3, which indicates that when the content of propionic acid in the medium is up to 1.0%, the cell growth of a new strain of propionic acid bacteria (in contrast to the original strain) is not inhibited and is characterized by a sufficiently large number cells. With a higher content of propionic acid (1.5%), a sharp decrease in the number of cells occurs and the morphology of the cells changes.

Table 3
The effect of the concentration of propionic acid on the growth of the strain Propionibacterium freudenreichii subsp. shermanii Ac-103/12 The name of the strain The concentration of propionic acid,% The number of cells in 1 ml of culture fluid Propionibacterium freudenreichii subsp. shermanii Ac-103/12 0
0.25 3.0 · 10⁸
1,0 · 10⁸ 0.5 5.0 · 10 ⁶ 0.75 1,0 · 10 ⁵ 1,0 2.0 · 10 ⁴ 1,5 1,0 · 10 ²

To propagate the strain, the selected largest colonies were subcultured onto a liquid nutrient medium of the following composition:

Distilled water 1000 ml

Yeast extract 10.0 g

KN ₂ PO ₄ 1.0 g

Na ₂ HPO ₄ · 2H ₂ About 3.0 g

CoSO ₄ 1.0 mg

Sodium Lactate (70% solution) 40.0 ml

Example 4

Strain Propionibacterium freudenreichii subsp. shermanii Ac-103/12 was evaluated by its ability to form an increased protein content by culturing it on a liquid nutrient medium of the composition described in Example 3, by measuring the amount of biomass and crude protein formed in the culture fluid.

An inoculum in an amount of five volume percent was introduced into flasks with a 750 ml liquid nutrient medium and cultivation was carried out under stationary conditions, subject to the following parameters: temperature 37 ° C, pH 6.0. A 20% sodium hydroxide solution was used as a neutralizing agent. After 24 hours of cultivation, bacterial growth and the amount of biomass and crude protein formed in the biosynthesis in the culture fluid were determined.

The results are presented in table 4.

Table 4
Characterization of the strain Propionibacterium freudenreichii subsp. shermanii Ac-103/12 Name of indicator Indicator value The number of cells in 1 ml of culture fluid 0.710 ⁹ The specific yield of the substrate, wt.% 95.0 Mass fraction of crude protein,% 47

Based on the results obtained, it was concluded that the selected strain has high productivity with respect to biomass accumulation (the initial content of bacterial cells was 1.0 · 10 ² ), a high specific yield from the substrate used and the accumulation of crude protein up to 47% versus 25% in the original strain.

Example 5

The strain Propionibacterium acnes Ac-1450/28 was obtained by repeated (at least 30) reseeding of cells of the original strain Propionibacterium acnes BKM Ac-1450 on a solid nutrient medium of the following composition:

Distilled water 1000 ml

Yeast extract 10.0 g

KN ₂ PO ₄ 1.0 g

Na ₂ HPO ₄ · 2H ₂ O 3.0 g

Agar 20.0 g

CoSO ₄ 1.0 mg

Sodium Lactate (70% solution) 40.0 ml

The content of free propionic acid in the medium was gradually increased from 0 to 1.5%. Cells were incubated in solid nutrient medium at 37 ° C for 24 hours. The selection was carried out according to the number of colonies and acid-forming ability, which was determined by titration. Those colonies were selected whose growth on a solid nutrient medium was not inhibited in the presence of 1.5% propionic acid. After 30 passages, the strain reached genetic resistance. The results of selecting a new strain of Propionibacterium acnes Ac-1450/28 by stepwise selection are shown in Table 5, which indicates that when the content of propionic acid in the medium is up to 1.0%, the cell growth of the new strain of propionic acid bacteria (unlike the original strain) does not inhibited and characterized by a sufficiently large number of cells. With a higher content of propionic acid (1.5%), a sharp decrease in the number of cells occurs and the morphology of the cells changes.

Table 5
The effect of the concentration of propionic acid on the growth of the strain Propionibacterium acnes Ac-1450/28 The name of the strain The concentration of propionic acid,% The number of cells in 1 ml of culture fluid Propionibacterium acnes Ac-1450/28 0 2.010 ⁸ 0.25 1,0 · 10 ⁷ 0.5 0.5 · 10 ⁶ 0.75 5.0 · 10 ⁴ 1,0 1,0 · 10 ⁴ 1,5 0.5 · 10 ²

To propagate the strain, the selected largest colonies were subcultured onto a liquid nutrient medium of the following composition:

Distilled water 1000 ml

Yeast extract 10.0 g

KN ₂ PO ₄ 1.0 g

Na ₂ NRA ₄ · 2H ₂ O 3.0 g

CoSO ₄ 1.0 mg

Sodium Lactate (70% solution) 40.0 ml

Example 6

The strain Propionibacterium acnes Ac-1450/28 was evaluated by its ability to form a high protein content by culturing it on a liquid nutrient medium of the composition described in Example 5, by measuring the amount of biomass and crude protein formed in the culture fluid.

An inoculum in an amount of five volume percent was introduced into flasks with a 750 ml liquid nutrient medium and cultured under stationary conditions and the following parameters were observed: temperature 37 ° С, pH 6.0. A 20% sodium hydroxide solution was used as a neutralizing agent. After 24 hours of cultivation, bacterial growth and the amount of biomass and crude protein formed in the biosynthesis in the culture fluid were determined.

The results are presented in table 6.

Table 6
Characterization of the strain Propionibacterium acnes Ac-1450/28 Name of indicator Indicator value The number of cells in 1 ml of culture fluid 0.6 × 10 ⁹ The specific yield of the substrate, wt. % 92.6 Mass fraction of crude protein,% 45.0

Based on the results obtained, it was concluded that the selected strain has high productivity with respect to biomass accumulation (the initial content of bacterial cells was 1.0 · 10 ² ), a high specific yield from the substrate used and the accumulation of crude protein up to 45% versus 25% in the original strain.

Example 7

A mixture of pure cultures of lactic acid and propionic acid bacteria is grown on the post-alcohol bard:

- Lactobacillus plantarum B 578/25 and Propionibacterium freudenreichii subsp. shermanii Ac-103/12;

- or Lactobacillus plantarum B 578/25 and Propionibacterium acnes Ac-1450/28.

At the end of the fermentation process, the resulting mass is separated into solid and liquid phases by filtration.

The solid phase is directed to drying at a temperature of (80-90) ° C. The obtained target product has a crude protein content of 40%, the amount of amino acids 35.5%.

The liquid phase - the filtrate is characterized by the following indicators: solids content of 1.7%, mass fraction of crude protein 0.69%, mass fraction of carbohydrates 0.39%, mass fraction of ash 0.25%.

Table 7 shows the indicators characterizing the liquid phase.

Table 7
Indicators characterizing the liquid phase Name of indicator The filtrate distilleries processed by a consortium of microorganisms Solids content,%
Mass fraction of nitrogenous substances,%
Mass fraction of ash,%
Mass fraction of fat,%
Mass fraction of carbohydrates,%
Mass fraction of ammonium nitrogen,%
The total amino acid content,%
The content of trace elements,%
The content of vitamins, mg / l
The content of lactate (propionate),% 1.01-1.7
0.104-0.11
0.13-0.25
0.0006-0.002
0.17-0.39
0.004-0.0055
0.29-0.31
0.105-0.135
101.3-156.5
0.2-0.5

The data presented in the table indicate that the filtrate of the culture fluid (treated vinasse) contains less solids (1-1.7%) compared to the original vinasse (5-6%), which allows the vinasse to be returned to the alcohol industry. At the same time, the presence in the processed stillage of nutrients (trace elements, vitamins, nitrogenous substances) stimulates the growth of alcoholic yeast.

The food product obtained after drying the solid phase contains 45.9% of crude protein and has the following composition:

Mass fraction of carbohydrates,% d.v. 44,2

Mass fraction of ash,% d.v. 2,5

total amino acid content,% 43.4

of them:

Lysine + histidine 2.15

Arginine 1.25

Aspartic acid 4.25

Threonine 2.0

Serene 2.08

Glutamic acid 14.84

Methionine + Cystine 1.3

Glycine 2.6

Proline 3.14

Phenylalanine + Tyrosine 2.65

Alanine 4.4

Isoleucine + Leucine 2.53

Mass fraction of protein according to Barstein,% by d.v. 37.1

Mass fraction of fat,% d.v. 5.1

total micronutrient content, mg / kg 21800

of them:

Phosphorus 5900

Potassium 3500

Sodium 500

Calcium 11600

Magnesium 1000

Iron 750

Cobalt 4.9

the content of vitamins, mg / kg:

E (tocopherol) 43.5

B ₁ (thiamine) 3.3

B ₂ (riboflavin) 7.7

B ₃ (pantothenic acid) 35.3

B ₄ (choline) 700

B ₅ (nicotinic acid) 26.8

B ₆ (pyridoxine) 8.2

B ₉ (folic acid) 18.0

B ₁₂ (cyancobalamin) 34.4

exchange energy, MJ 13.4

feed units, kg 1.37

The liquid phase obtained after filtration with a content in g / l:

Solids 10.1

Mass fraction of nitrogenous substances 1.04

Mass fraction of ash 1.3

Mass fraction of fat 0,006

Mass fraction of carbohydrates 1.7

Mass fraction of ammonia nitrogen 0.04

The total amino acid content,% 0.29

Total micronutrient content 1.05

Total Vitamin 1.01

The content of lactate (propionate), g / 100 ml 1.12

The resulting feed product contains living cells of lactic acid and propionic acid bacteria, enriching the intestinal microflora of animals consuming food, which leads to an increase in its biological value.

The liquid phase contains a small amount of solids, which are an additional source of nutrition for spirit yeast.

Thus, the method according to the invention allows to obtain a high-quality feed product, completely utilize the post-alcohol distillery stillage, reduce energy consumption and simplify the process itself, since it does not require heat treatment of the final product and aeration of the fermentation process, as in the production of fodder yeast. The liquid fraction returned to the previous stages of alcohol production has optimal physicochemical parameters and therefore does not adversely affect the alcohol fermentation process.

When filtering, a biomass is obtained, which is easily filtered mechanically, has a low moisture content, therefore, when it is dried, heat and energy consumption is reduced.

Claims (2)

1. A method of producing a feed product, including making wort, fermenting it to obtain mash, distilling the mash to produce ethanol and post-alcohol distillery stillage, growing microorganisms in the post-alcohol distillery stillage to obtain their biomass, separating biomass into solid and liquid fractions, and using the solid fraction as a feed product , characterized in that on post-alcohol bard grow pairs of strains of lactic acid and propionic acid bacteria: Lactobacillus plantarum B578 / 25 and Propionibacterium freudenreichii subsp. shermanii Ac103 / 12 or Lactobacillus plantarum B 578/25 and Propionibacterium acnes Ac1450 / 28. 2. The method of production of the feed product according to claim 1, characterized in that the liquid biomass fraction is used as recycled process water in the preparation of the wort.