RU2236854C1 - Disinfectant - Google Patents

Abstract

FIELD: veterinary science, medicine, pharmaceutics.

SUBSTANCE: the present innovation deals with water-soluble iodine-containing disinfecting preparations for disinfection and disinvasion of surfaces, equipment and housings. The suggested preparation is reaction product of iodine with complex-forming component at the presence of surface-active substance. Complex-forming component is monoalkylphenyl ethers, or oxyethylated alkylphenol ethers of polyethylene glycol, or polyoxyethyl ethers of synthetic primary fatty alcohols at the following ratio of initial components, weight%: crystalline iodine 10-20, complex-forming component 30-70, surface-active substance 15-20. As surface-active substance one should apply triethylene glycol or polyethylene glycol-200, or polyethylene glycol-400 or polypropylene glycol. The innovation enables to carry out disinfection at wider range of parasitic diseases' agents.

EFFECT: higher efficiency of application.

1 cl, 10 ex, 7 tbl

Description

The invention relates to the field of veterinary medicine, medicine and the pharmaceutical industry, in particular to water-soluble iodine-containing disinfectants used to disinfect and disinfect surfaces of equipment and premises of livestock complexes, indoor air, animals themselves and water. In addition, the tool can be used to treat the surgical and injection fields, to treat wounds, as well as for the treatment of respiratory and gastrointestinal diseases of animals.

In recent years, a number of drugs have appeared that have disinfectant and therapeutic properties, the active substance of which is iodine. A number of iodine-containing water-soluble preparations are known in which the dissolution of iodine in water is achieved by the inclusion of either potassium iodide (iodomidol, RU 2130312, 1999), or polyvinylpyrrolidone (iodovidone, RU 2145860, 2000), or hydrochloric acid in combination with potassium iodide, potassium iodide (RU 2075974, 1997), which significantly increases the cost of the tool. In addition, as a rule, the iodine content in known products is 1-5%.

Closest to the proposed one is the disinfectant described in patent RU 2161507, 1999. This tool is obtained by the complexation reaction of iodine with the copolymerization product of ethylene oxide and propylene obtained in the presence of a fraction of C ₁₂ -C ₁₄ alcohols. As a surfactant, polyethylene oxide-400 is added to the iodine-containing complex. In this case, the ingredients are taken in the following ratio: copolymer - 50-60%; polyethylene oxide-400 - 30-40% and crystalline iodine - 10-15%. At the same time, according to the description of the invention, the content of active iodine in the resulting product is only 7-10%, which may indicate that the complexation reaction does not take place to the end.

The drug, called pharmaiod, is highly soluble in water, has an antimicrobial effect against gram-negative and gram-positive bacteria, viruses and yeast-like fungi and is recommended for the treatment of surgical and injection fields.

The main disadvantage of the described disinfectant is a narrow spectrum of its action, in particular the lack of effectiveness against pathogens of the most resistant parasitic diseases.

To eliminate these drawbacks, it is proposed to use, as a complexing component, compounds of monoalkylphenyl ethers of polyethylene glycol, for example 35 (4-nonylphenoxy) 3, 6, 9, 12, 15, 18, 21, 24, 27, 30, 33 undecaoxapentatriacontan-1-ol, known under the name neonol AF 9-12, with the molecular formula C ₉ H ₁₉ C ₆ H ₄ O (C ₂ H ₄ O) ₁₂ N or ethoxylated alkyl phenol (monoalkyl) polyethylene glycol ethers based on polymer distillate or polyoxyethylene glycol ethers of synthetic primary fatty alcohols of fraction C ₁₂ -C ₁₄ (syntanol ALM-10). As additional surfactants, triethylene glycol, polyethylene glycol-200, polyethylene glycol-400 or propylene glycol are used. The complexation reaction is carried out at a temperature of 40-50 ° C for 1-3 hours.

To obtain a product - a product with a high content of active iodine, the following composition is proposed: crystalline iodine 10-20%; complexing component 30-70% and surfactant - 20-60%. The proposed conditions allow to obtain the final product with an output of active iodine of up to 95%. The resulting iodine-containing polymer complex compound, hereinafter referred to as farmayod-2, mixes well with water in all ratios, forming stable solutions. In practice, for the purposes of disinfection and disinvasion, aqueous solutions of the product with an active iodine content of from 0.003 to 5.0% are used.

According to the parameters of acute oral toxicity, the agent belongs to the III class of moderately hazardous substances. Vapors are not dangerous during inhalation exposure (IV class is low in volatility).

A distinctive feature of the claimed invention is the use as complexing agents of compounds of monoalkylphenyl ethers of polyethylene glycol, ethoxylated alkyl phenol ethers of polyethylene glycol or polyoxyethyl ethers of synthetic primary fatty alcohols, which allows to obtain the final product with a high content of active iodine. Moreover, the yield of the final product at the stage of complexation is at least 90%. The use of the proposed complexing agents gives the resulting agent a high antibacterial, antiviral and antifungal activity and can be used to disinfect injection and surgical fields. In addition, the proposed tool has disinvasive properties, causing the death of helminth eggs (for example, A. suum). The combination of disinfection and disinvasion properties of the proposed tool provides the ability to use it for processing equipment and facilities in livestock and poultry farms. The tool can be used for aerosol treatment to disinfect indoor air. In this case, the processing can be carried out in the presence of animals.

The inventive tool can be used to disinfect drinking water, and the disinfecting effect is observed for at least two weeks.

Information confirming the possibility of carrying out the invention

Example 1

Ingredients: crystalline iodine - 1.1 kg; neonol AF 9-12 - 6.9 kg; triethylene glycol - 2.0 kg.

The mass was loaded into a reactor with a jacket and a stirrer, heated to a temperature of 50 ° C and kept under stirring for 2-3 hours.

The content of active iodine in the finished product is 10.8%, which amounted to 98% of the introduced amount of iodine.

Example 2

Ingredients: crystalline iodine -1.5 kg; neonol AF 9-12 - 6.4 kg; polyethylene glycol-400 - 2.0 kg.

Preparation of funds was carried out as in example 1.

The content of active iodine in the final product is 15.1%, which amounted to 94% of the introduced amount of iodine.

Example 3

Ingredients: crystalline iodine - 1.0 kg; syntanol ALM-10 - 3.0 kg; triethylene glycol - 6.0 kg.

The tool was prepared, keeping the mass in the reactor at 40 ° C for an hour.

The content of active iodine in the finished product is 9.5%, which amounted to 95% of the introduced amount of iodine.

Example 4

Ingredients: crystalline iodine - 2.0 kg; syntanol ALM-10 - 6.0 kg; triethylene glycol - 2.0 kg.

The mass was kept in the reactor at 40-50 ° C with stirring for 2 hours. The content of active iodine in the finished product is 18.5%, which is 92.5% of the introduced amount of iodine.

Example 5

The antimicrobial, antifungal and antiviral activity of the samples of the agent was determined by the method of serial dilutions according to the instructions of the XI State Pharmacopoeia. The results of the determinations are given in table 1. The activity of the samples did not differ from each other.

Example 6

The effectiveness of disinfecting surfaces of laboratory equipment and injection and surgical fields are presented in Tables 2 and 3.

Example 7

Laboratory tests of pharmaiod-2 on the effects on A. suum eggs in laboratory conditions on Petri dishes were performed. When processing eggs with a 1% solution and exposure for 3 hours, 100% egg death was obtained compared to the control.

The tool was also tested on a culture of eggs A. suum in a concentration of 2; 3; 4 and 5% with exposure 3; 6 and 12 hours. After washing off the preparation, the culture was placed in a thermostat at 27 ° C (the conditions are optimal for the development of egg embryos). After exposure to the drug, the following structural changes in the membranes and plasma of egg embryos were noted: no blastomere crushing stages were noted; protoblast occupies the central part of the embryo structure or is shifted to the pole, has a dark brown color; egg shells turn dark brown. Control cultures of A. suum eggs developed in Petri dishes to the invasive stage, more than 90%.

Example 8

The disinvasive effect of pharmaiod-2 was tested in surface treatment (wood). Tested concentrations of solutions of 1 and 2%, exposure 3 and 6 hours. The flow rate of the solution is 1 l / m ² . Sampling from the surface was carried out by flushing and scraping. After filtering through a sieve and washing 3 times from the preparation, the egg suspension was transferred to Petri dishes and cultured for 24 hours.

Result: when treated with drug solutions with a concentration of 1% or higher, the number of eggs that developed to the larval stage was 1-2%. In the control of 96%.

Example 9

The proposed method of using pharmaiod-2 for disinfection and disinfestation was carried out on the farms of Kuibyshevskoye and Nesterovskoye on two pig-breeding and one cattle-breeding farms.

Processing of the premises was carried out in the presence of animals. Farmayod-2 was applied in the form of aqueous solutions with a concentration of 2; 1 and 0.5% of active iodine at an exposure of 3 and 6 hours. The flow rate of the solution is 0.7 l / m ² . For a comparative assessment of the effectiveness of the drug, 4 and 5% sodium hydroxide solutions were treated. The quality of the treatment was assessed by the presence of E. coli, staphylococcus and viable ectogenic forms of parasites (eggs, helminth larvae, cysts, protozoan oyster, tick eggs) in washed scrapings from surfaces. The effectiveness of disinvasion was evaluated by the effect of the tested solutions on the embryos of ascaris eggs, as the most stable of the ectogenic stages (forms) of parasites.

The processing results are shown in tables 4 and 5.

Example 10

The antibacterial and antifungal effect of pharmaiod-2 in water was checked as follows. Pharmaiod-2 was added to test tubes with water (10 ml each) to a final concentration of 0.025-0.003%. Then, 0.05 ml of microbial suspensions with a titer of 1 · 10 ⁹ cells / ml were added to the tubes and exposed at room temperature 20; 60; 120 and 180 min, after which they were inoculated from tubes onto a medium with meat peptone agar (bacterial culture) and glucose-potato agar (fungus culture). The test tubes were water with added test cultures.

The results of two parallel experiments are given in table.6.

The disinfecting properties of pharmaiod-2 solutions are maintained for at least two weeks (Table 7).

Claims (2)

1. A disinfectant, which is the product of the reaction of iodine with a complexing component in the presence of a surfactant, characterized in that the complexing component is monoalkylphenyl ethers, or ethoxylated alkylphenol ethers of polyethylene glycol, or polyoxyethyl ethers of synthetic primary fatty alcohols in the following ratio of starting components, .%: Crystalline iodine 10-20 Complexing component 30-70 Surfactant 15-20 2. The tool according to claim 1, characterized in that as a surfactant use triethylene glycol, or polyethylene glycol-200, or polyethylene glycol-400, or polypropylene glycol.