RU2228002C1 - Method for preparing of grain before laying for storage - Google Patents

Abstract

FIELD: agriculture. SUBSTANCE: method involves treating grain in superhigh frequency electromagnetic field by means of propionic acid including additive of chitosan, with source of chitosan adapted for adding into propionic acid being preparation obtained by sequential extracting of Pythium insidiosum micromycet biomass with the use of non-polar extractant in above-critical state, water, alkaline, water, acid, water, alkaline, and water, followed by joining of first extract with solid residue. EFFECT: reliable storage of grain. 2 ex

Description

The invention relates to the technology of post-harvest grain processing before laying for storage using organic preservatives.

A known method of preparing grain before laying it for storage, providing for its processing in the electromagnetic field of a microwave propionic acid containing an additive of chitosan (RU 2153793 C1, 08/10/2000).

The disadvantage of this method is the low reliability of grain storage, especially when secondary contamination is possible.

The technical result of the invention is to increase the reliability of grain storage.

This result is achieved by the fact that in the method of preparing the grain before putting it into storage, which involves treating it in an electromagnetic field with microwave propionic acid containing an additive of chitosan, according to the invention, a preparation obtained by sequential extraction of biomass of micromycete Rthium is used as a source of chitosan for addition to propionic acid insidiosum non-polar extractant in a supercritical state, water, alkali, water, acid, water, alkali and water, followed by combining the first strakta with the solid residue.

The method is implemented as follows.

The dry biomass of Pythium insidiosum micromycete is extracted with a non-polar extractant, for example carbon dioxide or hexane, in a supercritical state. At this stage, the first extract is isolated, which is used later in the preparation. Next, the biomass is sequentially extracted with water, alkali, water, acid, water, alkali and water. The solid residue obtained after completion of all the extraction steps listed above is combined with the first extract. Thus obtained drug is introduced into propionic acid. In an electromagnetic field, microwave grain is treated with propionic acid with the addition of the drug using one of the known methods, for example by spraying. The introduction into the propionic acid of a preparation from the biomass of Pythium insidiosum micromycete in an amount of more than 0.2% by weight is not recommended due to a sharp increase in viscosity, which prevents uniform application to the grain. The influence of the microwave field and propionic acid leads to the destruction of the surface microflora of the grain. The composition of the biomass micromycete Pythium insidiosum consists mainly of chitosan and higher polyunsaturated fatty acids, including arachidonic and eicosapentaenoic. The latter, in combination with chitosan, have a synergistic effect on stimulating the protective reactions of plants. At the same time, the drug does not include substances that can inhibit this activity, and phytotoxic substances. As a result, the grain is deposited with induced resistance, which can inhibit the development of phytopathogenic microflora during secondary contamination, even in conditions of high infection density.

Example 1

Before laying for storage, grain of Skifyanka wheat is treated in the microwave field with propionic acid containing 0.01% by weight additive of chitosan or a preparation obtained from the biomass of Pythium insidiosum micromycete using carbon dioxide as a non-polar extractant and alkali sodium hydroxide and of hydrochloric acid. After 1 month of storage, grain samples are taken from the experimental and reference lots, which are contaminated with spores of fungi of the genera Aspergillus and Penicillium in an amount of 2 · 10 ¹² spores / mg. By the end of 6 months of storage in the experimental samples, the development of molds was not detected, on all reference samples developed mycelium of molds was found.

Example 2

Before laying for storage, Spalchik rice grain is treated in the microwave field with propionic acid containing an additive in the amount of 0.2% by weight of chitosan or a preparation obtained from the biomass of Pythium insidiosum micromycete using hexane as a non-polar extractant, as an alkali, ammonium hydroxide and propionic acids. After 1 month of storage, samples are taken and contaminated according to example 1. The result is the same as example 1.

Thus, the proposed method improves the reliability of grain storage.

Claims (1)

A method of preparing grain before putting it into storage, providing for its processing in a microwave electromagnetic field with propionic acid containing a chitosan additive, characterized in that a preparation obtained by sequential extraction of Pythium insidiosum micromycete biomass with a non-polar extractant in supercritical is used as a source of chitosan for addition to propionic acid condition, water, alkali, water, acid, water, alkali and water, followed by combining the first extract with a solid residue.