RU2227027C1 - Method for treatment of hepatodystrophy in piglet-weanlings - Google Patents

Abstract

FIELD: veterinary science. SUBSTANCE: method involves application of calcium pantothenate in the dose 10 mg/kg of fodder in combination with carnitine in the dose 15-35 mg/kg of fodder. Method provides the enhancement of effectiveness of treatment by 64%. EFFECT: improved treatment method. 3 tbl, 2 ex

Description

The invention relates to veterinary medicine and can be used to treat hepatodystrophy in weaned piglets.

Hepatodystrophy syndrome has become widespread among registered pathologies of pigs; the incidence of weaned piglets reaches 76%. This pathology is a systemic metabolic disorder that occurs with a failure of adaptation and leads to destructive changes in the liver.

A known method of treating hepatodystrophy of piglets using sodium selenite, dipromonium / 1 /. Promising is the treatment of hepatodystrophy in piglets with the use of vitamin-containing preparations - diprovit, diproanemin, metavit, lipovit C, vitamins U, E, etc. / 2, 3 /.

The closest analogue is a method of treatment using calcium pantothenate, which is a calcium salt of pantothenic acid (vitamin B ₃ (formula (HOCH ₂ C (CH ₃ ) ₂ CH (OH) CONH (CH ₂ ) ₂ COO) ₂ Ca) / 4 / .

However, the known methods of treatment in conditions of high environmental and technogenic load on the body of piglets are not effective enough, since they act only on one link in the pathogenesis of the development of hepatodystrophy in piglets.

The purpose of the invention is improving efficiency.

The presented goal is achieved by the use of calcium pantothenate in combination with carnitine.

Carnitine (Vitamin W, Betaine-3-hydroxy-4-trimethylaminobutyric acid) is a widely used biologically active substance. The carnitine coenzyme is involved in the transfer of long chain fatty acids to mitochondria for β-oxidation with the formation of acetyl-CoA (coenzyme of acetylation). Therefore, carnitine acts as one of the most important metabolic regulators.

The method is as follows.

Piglets use calcium pantothenate at a dose of 10 mg / kg of feed in combination with a stable form of natural carnitine - L-carnitine tartrate (formula C ₁₈ H ₃₂ N ₂ O ₁₂ ) - with a carnitine content of 68.2% in a daily dose of 15-35 mg / kg of dry food for 28 days. The most effective is the use of carnitine in a dose of 25 mg / kg of dry food. Food mixed with calcium pantothenate and carnitine is given once a day at the rate of 1 kg of dry food per head.

The combined action mechanism of the claimed vitamin preparations is based on their biological role. Carnitine has a direct effect on the formation of acetyl-CoA, the main substrate of the respiratory cycle, in the mitochondria of hepatocytes. PAA is an integral part of the carrier of acyl groups - CoA, whose biological role includes participation in more than 70 enzymatic reactions. The reactive region — the SH group — contained in the PAA molecule activates the intermediate exchange of products of the respiratory cycle and the oxidation of fatty acids, restoring the metabolism impaired by hepatodystrophy.

The effectiveness of the treatment of hepatodystrophy of piglets with the use of calcium pantothenate and carnitine was determined in the conditions of the 108 thousandth pig complex “Slobogansky Agricultural Complex” in the Kharkov region of Ukraine. 55-day-old pigs of weaning and growing of large white breed, live weight of 9.2-10.8 kg, were examined. Animals received the same rations, balanced in nutrients in accordance with standard norms.

The essence of the method is illustrated by examples.

Example 1

To determine the effectiveness of the claimed and known methods of treating hepatodystrophy, 42 piglet heads were examined for 28 days. Piglets, patients with hepatodystrophy, in the amount of 14 goals in each group were selected on the basis of analogues, taking into account the clinical manifestation of the disease. The conditions of feeding and feeding were identical. For the experiment, three groups were formed, which were received with food: 1st — calcium pantothenate at a dose of 10 mg / kg of feed; 2nd — calcium pantothenate at a dose of 10 mg / kg of feed in combination with carnitine at a dose of 25.0 mg / kg of feed per day (optimal dosage); group 3 pigs were intact (did not receive drugs).

Data on therapeutic efficacy, determined by the number of recovered animals, are given in table 1.

The best results were obtained in the treatment of piglets with hepatodystrophy, a combination of pantothenic acid preparations at a dose of 10 mg / kg feed and carnitine at a dose of 25 mg / kg feed once a day for 28 days.

Example 2

To justify the therapeutic efficacy of hepatodystrophy in piglets of the three groups studied in Example 1, after 28 days of the experiment, morphobiochemical blood parameters and histochemical liver parameters were determined (tables 2, 3).

The main criteria for the reliability of the therapeutic effect of the combination of pantothenic acid and carnitine preparations in the declared doses were biochemical blood tests and histochemical studies of the liver of weaned piglets with hepatodystrophy, carried out at the end of the experiment.

Analysis of tables 2 and 3 showed that the use of calcium pantothenate at a dose of 10 mg / kg of feed and carnitine at a dose of 25 mg / kg of feed for piglets for therapeutic purposes led to increased metabolic utilization of carbohydrates and lipids (blood acetylation ability, β-lipoprotein content), and the additional introduction of calcium pantothenate into the diet increased the throughput of the Krebs cycle (succinate dehydrogenase). The administration of drugs contributed to the removal of hepatosuppressive (total bilirubin) and cytolytic (AlAT, AsAT) syndromes, the gradual restoration of secretory (glycogen, total proteins) and excretory functions of the liver (direct bilirubin), and a decrease in the degree of fatty liver infiltration (neutral fats).

The novelty of the claimed method lies in the use of a combination of calcium pantothenate and carnitine in a dose: calcium pantothenate -10 mg / kg feed, carnitine 15-35 mg / kg feed. The unknown of the proposed scheme, which allows to reduce the incidence to 36%, indicates the compliance of the claimed technical solution with the criterion of "inventive step". Feasibility and effectiveness are confirmed in scientific experience.

Sources of information taken into account:

1. Hepatoses of farm animals and hepatoprotective drugs: Guidelines for the diagnosis, treatment and prevention of hepatoses, agricultural animals / Kuznetsov N.I., Nikulin I.A., Visloguzov A.M. et al. / VGAU. VNIVIPFiT. - Voronezh, 2001 .-- 65 p.

2. The use of complex hepatopathic and vitamin preparations in industrial pig breeding / Ed. N.I. Kuznetsova. - Voronezh, 1990 .-- 7 p.

3. Meleshkina S.R. Features of dynamics of indicators of hematological and biochemical status of piglets in the appointment of hepatotropic drugs: Author. dis ... cand. biol. sciences. - Voronezh, 1995 .-- 24 p.

4. Slobodyanik V.S. Metabolism of pantothenic acid metabolism in pigs with liver dystrophy, parakeratosis and transport stress: Abstract. dis ... cand. biol. sciences. - Voronezh, 1983 .-- 20 p.

Claims (1)

A method of treating hepatodystrophy in weaned piglets, including the use of calcium pantothenate, characterized in that carnitine is additionally used in a dose of 15-35 mg per 1 kg of dry food.