RU2196739C2 - Sulfate-reduction bacteria growth control agent - Google Patents

Abstract

FIELD: bacteriostatics. SUBSTANCE: agent, in particular for use in petroleum industry, is N-o- ethoxyphenyl-perhydro-1,3-5-dioxazine with general formula C₁₁H₁₅O₃N. EFFECT: increased biocidal efficiency at zero toxicity and anticorrosive effect. 1 tbl

Description

 The invention relates to the field of organic chemistry, in particular to substances with the ability to suppress the vital activity of bacteria, and can be used to prevent the growth of sulfate-reducing bacteria (SBA) in various technological environments, in particular, in the oil industry.

 Known substances that inhibit the development of SVB, such as chlorophenols / [1] US Patent 3288211, 1966 /. Chlorophenols are added to water during waterflooding in the process of oil production.

 The disadvantage of this method of combating SVB is the low solubility of chlorophenols in water and high in oil, which has a negative effect on oil refining processes due to poisoning of the catalysts of these processes. In addition, chlorine-containing compounds threaten possible corrosion of equipment.

 NALCO uses a biocide from ROBAN 3999, a substance called 2-methyl-5-nitroimidazole-1-ethanol, to suppress the growth of SVB. / [2] Industrial catalog of the company NALCO Chemical; EI, a series of "Technique and technology of oil production and the development of oil fields", foreign experience, 1988. , 7 /. The disadvantages of ROBAN 3999 include the feature that, over time, the effectiveness of the biocide on bacteria decreases, since the basis of the mechanism of their interaction with ROBAN 3999 is a metabolic disorder, and a decrease in the metabolic rate in bacteria leads to a decrease in the ability to absorb the biocide. As a result, the treatment of the injected water with the ROBAN 3999 biocide is carried out in several stages for several weeks, and the dosage of the reagent is calculated each time based on the varying degree of activity of anaerobic bacteria, which is very difficult in operational practice.

 Derivatives of amino compounds, for example, 3-tert-butyltetrahydro-1,3-oxazine / [3] A., have a high biocidal activity. USSR 791620, 1980 /. This substance completely inhibits the growth and development of SVB at a concentration of 0.01% (100 mg / l). However, the specified tool is not available; its synthesis is difficult and expensive, which inhibits its widespread use.

Синтез N-o-этоксифенил-пергидро-1,3,5-диоксазин (далее ЭФДОА) осуществляют по известной методике /[4] Дж. Уокер "Формальдегид", М., ГХИ, 1957/ из формалина и о-фенетидина (о-этоксифениламина) в стехиометрических соотношениях в нейтральной среде при комнатной температуре. Полученный водный раствор ЭФДОА используется в качестве биоцидного средства без выделения и дополнительной очистки.The inventive biocidal reagent - No-ethoxyphenyl-perhydro-1,3,5-dioxazine General formula

The synthesis of No-ethoxyphenyl-perhydro-1,3,5-dioxazine (hereinafter referred to as EFDOA) is carried out according to the known method / [4] J. Walker "Formaldehyde", M., GHI, 1957 / from formalin and o-phenethidine (o-ethoxyphenylamine ) in stoichiometric ratios in a neutral medium at room temperature. The resulting aqueous solution of EFDOA is used as a biocidal agent without isolation and additional purification.

The effectiveness of using EFDOA as a reagent to suppress the growth of SFS was determined by the method of [5] Postgatz JR The sulfat-reducing bacteria. Cambridge University Press, 1979 /. Said reagent was introduced into commercial water containing SVB under sterile anaerobic conditions and kept for 24 hours at 32 ^° C. Then, 1 ml of liquid was taken from these samples in Postgate nutrient vials and thermostated for 15 days at 32 ^° C. As a control, similar samples were used without the addition of reagents. Evaluation of the bactericidal activity of the reagent was carried out according to the degree of suppression of SVB, determined by the amount of hydrogen sulfide formed during 15 days in experimental and control samples.

 EXAMPLE 1. N-o-ethoxyphenyl-perhydro-1,3,5-dioxazine.

In a reactor equipped with a thermometer, reflux condenser, stirrer and dropping funnel, thermostatically controlled at 20 ^° C, 1 M formaldehyde is added in the form of an aqueous solution and 1 M o-phenethidine (o-ethoxyphenylamine) is added dropwise with stirring. The resulting solution containing up to 70% EFDOA, without further processing, is used as a biocidal agent.

EXAMPLE 2. The formation water model was freed from oxygen, infected with a culture of sulfate-reducing bacteria isolated from oilfield formation waters, and treated with a reagent in the form of an O, 5% solution in certain dosages. The samples were held for 24 hours at 20-22 ^° C, after which part of the processed sample was introduced into a nutrient medium, placed in a thermostat and incubated for 15 days at 35 ^° C. The effectiveness of the reagent was evaluated by the appearance or absence of hydrogen sulfide in the solution. The presence of hydrogen sulfide was indicated by a solution of iron salt by the release of a black precipitate.

 The test results are shown in the table.

 As can be seen from the table, the inventive tool has a high efficiency of suppressing SVB, surpasses the well-known and widely used in oil production reagent "Don-52".

 The simplicity of the synthesis of EPDOA, the low cost and availability of raw materials for the production of EPDOA are obvious advantages over the 3-tert-butyltetrahydro-1,3-oxazine reagent with the same high biocidal efficiency.

The advantages of the claimed funds are also
- good solubility in water and weak in oil;
- manifestation of corrosion inhibiting properties;
- non-toxicity to humans and animals;
- explosion and fire safety.

Claims (1)

Means for inhibiting the growth of sulfate-reducing bacteria, characterized in that N-o-ethoxyphenyl-perhydro-1,3,5-dioxazine of the general formula C ₁₁ H ₁₅ O ₃ N is used as the active active ingredient.

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