RU2180169C2 - Method to protect plants against bacterial and fungous diseases - Google Patents

Abstract

FIELD: agriculture, biological methods of plant protection. SUBSTANCE: a vegetating plant is sprayed up to its full wettability with a fungicide which represents a protozoa culture isolated out of a healthy plant of the same variety as an infected one belongs to. A solution is prepared by boiling in the water a cut dry leaf-stalk plant mass of the same variety as an infected one is, cooling and removal of plant residues. The solution obtained is supplied with potassium-phosphate buffer at 7.5 pH. The mixture is kept for 3 d, then freshly cut stalks of a healthy plant of the same variety are added and the mixture is kept for about 3-5 d till the development of protozoa starts in the solution. The latter is efficient in protecting against tomato and potato phytophthora infection agents, cucumber perinosporosis and cabbage bacteriosis. EFFECT: higher efficiency of plant protection.

Description

 The invention relates to biological plant protection products against bacterial and fungal diseases.

 Known methods of using bacterial, fungal and actinomycete preparations for controlling pathogens of bacterioses and mycoses of plants. ("The bacterial strain Pceudomonas fluorescens to obtain a preparation against fungal diseases of plants." Description of the invention to patent SU 1817874, CL A 01 N 63/00, C 12 N 1/20, 05/20/95; "The strain of the fungus Gliocladium catenulatum Gilmann et Abbott to obtain a preparation against fungal pathogens of potato. "Description of the invention to the copyright certificate SU 1836907, class A 01 N 63/00, C 12 N 1/14, 08/30/93;" The strain of actinomycete Streptomyces violaniger to obtain a nigrocide active against phytopathogenic microflora " , CL A 01 N 63/00, C 12 N 1/14, 02/07/93).

 The aim of the invention is to increase the effectiveness of the biological method of combating bacterial and fungal diseases of plants.

 The method consists in spraying a vegetative plant to its full wettability with a biopesticide, which is a culture of several species of protozoa isolated from a healthy plant of the same variety as the infected plants. The cultivation of protozoa is carried out on a nutrient medium prepared from a dry leaf-stem mass of plants of this variety with the addition of an alkaline buffer solution.

 Example.

 The culture medium for protozoa was prepared as follows: 15 g of dry crushed leaf-stem mass of plants of the same variety as those affected by the disease were boiled for 15 min in 1 l of distilled water. Then the solution was cooled to room temperature, filtered from plant residues, poured into a flask, 50 ml of potassium phosphate buffer (pH 7.5) was added, closed with a cotton swab and left for 3 days until a bacterial film appeared on the surface of the solution.

 The environment was populated with protozoa by introducing into the medium part of a freshly cut stem of a healthy plant of this variety.

 After 3-5 days, microscopic control was performed. The development of populations of protozoa testified to the suitability of the solution for use.

 Spraying of infected plants was carried out using a spray gun, eliminating the effect of high pressure on the working fluid.

 Protozoa cultures have shown positive results in the fight against pathogens of late blight of potatoes and tomatoes, perinosporosis of cucumber, cabbage bacteriosis.

Claims (1)

 A method of combating pathogens of bacterial and fungal diseases of plants by spraying a vegetative plant until it is completely wetted with a bactericide (fungicide), characterized in that as a bactericide (fungicide) a solution is used containing populations of protozoa prepared by boiling in a crushed dry leaf-stem mass of a plant the same varieties as that affected by the disease, cooling the resulting solution, removing plant residues from it, adding a pH of 7.5 to the solution of potassium phosphate buffer, withstanding Ia mixture for 3 days before the bacterial film on the surface, and checking the solution by simply making freshly cut stem of healthy plants of the same variety followed by aging the mixture for 3-5 days.