RU2171116C2 - Neurotropic medicinal agent - Google Patents

Abstract

FIELD: medicine. SUBSTANCE: invention proposes the potentiated form of brain-specific protein S-100 taken in hundredth dilution C1-C10000. Agent shows the broad spectrum of action (sedative, tranquilizing, nootropic, psychoactive, antipsychotic, antiabstinent in alcoholism and narcomania). Agent can be used for correction of damaged immune homeostasis accompanying with alteration of psychosomatic functions and/or biological responses based on multiple effect on integrative activity of brain. EFFECT: enhanced effectiveness of agent, valuable medicinal properties, absence of adverse symptoms. 2 cl, 7 ex

Description

 The invention relates to medicine and can be used to correct impaired immune homeostasis, accompanied by a change in psychosomatic functions and / or biological reactions, through a multifaceted effect on the integrative activity of the brain.

 The immunological methods of treating various diseases by using drugs based on the antigen that causes these diseases are widely known (see, for example, Clinical Immunology, edited by L. Yeager, M .: Medicine, 1990, V.1, C 204-208, auth. Of the USSR 1131508, class A 61 K 39/00, 1984; auth. Of the USSR 1169659, class A 61 K 39/00, 1985; auth. 1178450, CL A 61 K 39/00, 1985; RF patent 2098186, CL A 61 K 39/02, 1997; RF patent 2092184, CL A 61 K 39/00, 1997) However such drugs are ineffective, especially in the treatment of immunodeficiency diseases with an unknown etiology.

 Closest to the invention can be considered the use of drugs based on brain-specific proteins as a neurotropic drug (see the book by MB Stark, Brain-specific proteins (antigens) and neuron functions, M .: Medicine, 1985, pp. 224-225 ) These drugs are applicable for the treatment of certain diseases of the central nervous system (schizophrenia, manic-depressive psychosis, epilepsy), but their effect is not well understood and may cause side effects.

 The invention is aimed at creating an effective neurotropic drug based on a brain-specific protein with a wide range of effects (sedative, tranquilizing, nootropic, psychoactive, antipsychotic, anti-withdrawal in alcoholism and drug addiction) without side effects with minimal amounts of the original brain-specific protein.

 The solution to this problem is provided by the fact that a neurotropic drug based on a brain-specific protein is a potentiated form of a brain-specific protein S-100 with a molecular weight of 21,000 D, obtained by repeated dilution and shaking according to the homeopathic method. In addition, the potentiated form is prepared in hundredth dilutions of C1-C10000.

For the preparation of the claimed neurotropic drug, a brain-specific protein S-100 is used (the physicochemical properties of which are described in the article by M.V. Starostin, S.M.Sviridov, Neurospecific protein S-100, Successes in modern biology, 1977, v. 5 , p. 170-178; in the book by MB Stark, Brain-specific proteins (antigens) and neuron functions, M .: Medicine, 1985, p. 12-14), isolated from the tissue of the brain of a bull by the following method:
- fabric frozen in liquid nitrogen is ground into powder in a special mill;
- the extraction of proteins is carried out in a ratio of 1: 3 (weight / volume) in the extraction buffer during homogenization;
- the homogenate is heated for 10 min at 60 ^o C and cooled to 4 ^o C in an ice bath;
- thermolabile proteins are removed by centrifugation;
- carry out stepwise fractionation with ammonium sulfate, followed by removal of precipitated impurity proteins;
- the S-100 protein-containing fraction is precipitated with 100% saturated ammonium sulfate when the pH is reduced to 4.0 and collected by centrifugation;
- the precipitate is dissolved in a minimum volume of a buffer containing EDTA imercaptoethanol, dialyzed against deionized water and freeze-dried;
- fractionation of acidic proteins is continued by chromatography on ion-exchange media - DEAE-cellulose DE-52 and then DEAE-Sephadex A-50;
- collected and dialyzed fractions containing S-100 protein are separated by molecular weight by gel filtration on Sephadex G-100;
- purified S-100 protein is dialyzed and freeze-dried;
The molecular weight of the purified brain-specific protein S-100 is 21,000 D.

 Due to the high content of aspartic and glutamic acids, the brain-specific protein S-100 is strongly acidic and occupies the extreme anode position during electrophoresis in a discontinuous buffer system in a polyacrylamide gel, which facilitates its identification.

 From the obtained brain-specific protein S-100, which is a substance soluble in a neutral solvent (distilled water, physiological saline, alcohol, etc.), a potentiated form is prepared, while the concentration is uniformly reduced by successive dilution of 1 part of the substance in 9 parts (for decimal dilution) or in 99 parts (for hundredth dilution) or 999 parts (for thousandth dilution) of a neutral solvent with multiple vertical shaking ("dynamization her ") of each dilution obtained and using separate containers for each subsequent dilution to obtain the required potency - dilution ratio according to the homeopathic method (see, for example, V. Schwabe" Homeopathic Medicines ", M., 1967, p. 14- 29). The neurotropic drug thus prepared is used in dosage forms and dilutions accepted in homeopathic practice, predominantly one hundred (C1-C10000), in the form of alcohol or aqueous solutions or tablets (granules) obtained by impregnation of potentized cane sugar (glucose) grains with potent until saturated solution to stimulate regenerative processes in the treatment of disturbed various psychosomatic functions and biological reactions.

 Example 1

 Patient S., 38 years old. He was admitted to a drug treatment hospital with a diagnosis of alcoholism of the II stage, acute alcohol withdrawal. I received the claimed neurotropic drug - a potentiated brain-specific protein S-100 in homeopathic dilution of C200 in the form of alcohol drops: 10 drops in a tablespoon of water every 30 minutes inside (without the use of other medicines). The condition improved: dyspeptic disorders, a feeling of weakness disappeared, sleep, appetite, and mood returned to normal. After 4 days, was discharged in satisfactory condition. No craving for alcohol.

 Example 2

 Patient D., 40 years old. She was admitted to the Department of Neurosis with a diagnosis of endogenous depression of unknown etiology. Complains of lethargy, indifference to the environment, a feeling of anxiety in the morning. Periodically - unwillingness to live, thoughts of suicide. I took the claimed neurotropic drug - potentiated brain-specific protein S-100 in the form of tablets of 0.5 g at a dose of C1000. Over the course of a week, the condition improved markedly: the mood evened out, did not feel anxiety in the mornings, notes that the dream was fully restored, there were no thoughts about death. 10 days after the start of treatment was discharged in satisfactory condition. I did not receive other antidepressants.

 Example 3

 Patient K., 28 years old. Suffers from neurasthenia. Over the years, observed at the local psychiatrist. Complains of fatigue, inability to cope with mental and physical stress, disbelief in one's own strengths. Against the background of daily intake of 1 tablet of 0.5 g of potentiated brain-specific protein S-100 at a dose of C50 in the morning for 6 months, it notes an increase in mental activity, normalization of sleep, the disappearance of anxiety, fatigue, and weakness. Considers himself practically healthy. Cope with the professional duties of a design engineer and homework.

 Example 4

 Patient N., 24 years old., Student. After a tense session notes fatigue, weakness, inability to concentrate, due to psychasthenic disorders can not cope with mental stress. The course of traditional psychostimulants and nootropics did not bring any effect. After prescribing the claimed neurotropic drug in the form of a solution at a dose of C1000 (5 drops in a tablespoon in the morning and at lunch before meals), it notes a good concentration, activity, vigor, and easily copes with the training load.

 Example 5

 Patient I., 30 years old. Diagnosis: low-grade schizophrenia. The disease manifests itself in scanty neurosis-like symptoms, a decrease in the person’s energy level, indifference to others and his own fate, inadequate judgments, and a tendency to philosophize. Despite having a higher legal education, he doesn’t work, he spends time aimlessly. For 6 months, I took 1 tablet daily, 0.5 g of potentiated brain-specific protein S-100 at a dose of C50. The condition has improved markedly. He became more adequate, directed, expanded the range of interests. He managed to get a job as a lawyer-consultant at the enterprise (with part-time work), normal relations with parents were restored.

 Example 6

 Patient R., 18 years old. Diagnosis: opium addiction 1 tbsp. The state of remission. The last time I took the drug was 3 months ago: one injection of heroin in the usual dose for the patient. Prior to this, he underwent a rehabilitation course in a hospital. The patient learns, copes with the training load, but dysphoric conditions with a pronounced attraction to the drug are periodically noted. On such days, takes 1 tablet of 1.0 g of the claimed neurotropic drug - potentiated brain-specific protein S-100 3 times a day at a dose of C1000. The patient notes that irritability disappears, and the attraction to the drug decreases.

 Example 7

 Patient L., 29 years old. Diagnosis: schizophrenia is fur-like. Is in the hospital for the 3rd time in the last 10 months. Acute hallucinatory-paranoid symptoms of the persecutorial plan are noted, which are extremely resistant even to large doses of traditional antipsychotics. It was possible to stop psychotic symptoms only after joining the claimed neurotropic drug, the potentiated brain-specific protein S-100, in a C1000 dilution of 5 drops of an alcoholic solution 6 times a day.

Claims (2)

 1. A neurotropic drug based on a brain-specific protein, characterized in that it contains a potentiated form obtained by successive multiple dilutions and shaking according to the homeopathic method from a brain-specific protein S-100 with a mol. Of 21000 D.  2. The neurotropic drug according to claim 1, characterized in that it includes a potentiated form of a brain-specific protein in hundred-percent dilutions of C1-C10000.