RU2157700C2 - Virus-vaccine against rabies for oral immunization of carnivore animals - Google Patents

Abstract

FIELD: veterinary sciences, vaccines. SUBSTANCE: invention relates to agents used for specific prophylaxis of carnivore animals against rabies. Vaccine has the sublimated dried suspension of the virus strain TC-80 and stabilizing agent. Vaccine has peptone, lactose and pectin as stabilizing agents protecting virus from inactivating effect of gastric juice and environment factors. After two-fold vaccination with pause for 12-14 days vaccine stimulates production of virus-neutralizing antibodies in vaccinated animals in the range (1:16)-(1:32). Invention ensures to obtain sublimated vaccine against rabies used for immunization of carnivore animals by oral route. Invention is designated for specific prophylaxis of rabies in carnivore animals. EFFECT: enhanced effectiveness of vaccine. 2 ex

Description

 The invention relates to the field of veterinary virology, namely to the production and use of biological preparations for oral immunization of carnivores against rabies.

 There is a known dry vaccine for oral immunization of wild carnivores from strain RB-71, which is rehydrated and injected into bait in the form of pieces of fish, meat or chicken heads before use (Abstracts of reports of the international symposium: "Problems of pathology and protection of the health of wild animals, ecological interaction of diseases wild and farm animals, Moscow, 1992).

 However, the procedure for preparing baits with a vaccine immediately prior to vaccination complicates the vaccination process, and the short shelf life of the vaccine in such baits reduces the effectiveness of immunization.

 There is a rabies vaccine ready for oral use, which consists of a liquid suspension of rabies virus, a strain of "GARDEN" placed in the internal cavity of the bait in the form of a briquette (Temporary instruction on the use of the drug "Lysvulpen" for immunization of wild animals (in the production test in 1998 -2000), approved by the Veterinary Department of the Ministry of Agriculture of the Russian Federation on March 26, 98, N -13-3-2 / 1197). The disadvantage of this vaccine is that it is unstable at positive temperatures, which makes it ineffective to use it in the summer-autumn period to immunize young carnivores.

 Also known is a vaccine from strain TC-80 (Ru 2077338 Cl, A 61 K 39/208). The vaccine strain TS-80 is antigenic, stable, does not have immunogenicity variability for various animal species, and was deposited at VGNKI on 02.17.88 (Register No. 10B). The vaccine is obtained by growing the virus in a transplanted culture of saiga kidney cells in a serum-free culture medium, then A-24 (1,8,3,6-diendomethylene-1,3,6,8-tetraazacyclodecane) is inactivated and adsorbed onto aluminum hydroxide. The disadvantage of this vaccine is that with oral immunization it does not have immunological effectiveness, since the antigen is destroyed by the action of gastric juice.

 The aim of the present invention is a rabies vaccine virus in the form of a dry polydisperse powder, freeze-dried, immunologically effective for oral immunization.

 This goal is achieved by the fact that the suspension of rabies virus, strain TS-80, is freeze-dried, pre-mixed with components that protect the virus from the inactivating effect of gastric juice and environmental factors, grind in a ball mill and fill the briquette bait. Protective components are peptone, lactose and pectin.

A positive effect is achieved with the following ratio of components, wt. %:
rabies virus suspension, strain TS-80 - 5-7
peptone - 65 - 67
lactose - 11 - 13
pectin - the rest
Case Studies
Example No. 1.

1000 ± 10 cm of rabies virus suspension, strain TS-80 grown in a transplanted saiga kidney cell culture is mixed with an equal volume of stabilizing medium containing 40 ± 2% peptone and 8.0 ± 1.0% lactose, an equal volume is added to the mixture 4.0 ± 0.5% pectin, the resulting technical fluid is subjected to freeze-drying. The dry vaccine is ground in a ball mill, biological activity, sterility and safety are determined, then bait briquettes are filled, which are stored at a temperature of 6-8 ^o C. until use.

 Serological studies of blood serum samples of carnivores immunized twice with an interval of 12 days revealed titers of neutralizing antibodies in foxes and raccoon dogs from 1:16 to 1:32 2 months after vaccination. Titles of pre-immune serum samples did not exceed 1: 2.

 Example No. 2.

500 ± 5 cm of rabies virus suspension, strain TS-80 grown in a transplanted saiga kidney cell culture is mixed with two volumes of 4.0 ± 0.5% pectin and one volume of a stabilizing medium containing 35.0 ± 0.5 % peptone and 8.0 ± 1.0% lactose. The resulting technical fluid is poured into 150 cm vials and freeze-dried. Dry vaccine is crushed by a roller, characteristics are determined and bait briquettes are filled. Bait with the vaccine is stored at a temperature of 6-8 ^o C until use.

 Serological studies of blood serum samples of carnivores immunized twice with an interval of 14 days showed the presence of virus-neutralizing antibodies in foxes and raccoon dogs in titers from 1:16 to 1: 32 2 months after vaccination, while titers of pre-immune serum samples did not exceed 1: 2 .

 The data presented indicate a high immunological efficacy of the dry virus vaccine from strain TS-80 for oral immunization of carnivores.

Claims (1)

Rabies virus vaccine for oral immunization of carnivores, freeze-dried, containing a suspension of rabies virus, strain TS-80, characterized in that it further comprises peptone, lactose and pectin in the following proportions, wt.%:
Suspension of rabies virus strain TS-80 - 5 - 7
Peptone - 65 - 67
Lactose - 11 - 13
Pectin - Else