RU2152804C1 - Disinfectant - Google Patents

Abstract

FIELD: disinfectants with washing effect. SUBSTANCE: disinfectant comprises bactericidal component, washing liquid substance, isopropyl alcohol and water taken in preset quantitative ratio. The disinfectant does not contain aggressive and explosive substances which are dangerous at manufacture and using. EFFECT: retention of activity for prolonged time. 7 tbl, 7 ex

Description

 The invention relates to sanitation, namely to disinfectants.

 A disinfectant is known including peracetic acid, secondary alkyl sulfates, sodium tripolyphosphate and water. The active ingredient in the composition is extremely explosive, unsafe in handling peracetic acid. In addition, this composition decomposes during storage and loses its activity (SU 1532051, 12/30/1989).

 The task is to develop a disinfectant that includes a less aggressive component, low risk during manufacturing and use, long-lasting microbiological activity and has a wide spectrum of action (E. coli, Staphylococcus aureus, Anthracoid spores, Pseudomonas aeruginosa, Proteus, micrococcus, fecal streptococcus, Candida albicans tubercle bacillus, poliomyelitis virus type 1) and detergent effect.

The problem is solved in that the disinfectant includes an active agent: surfactant, water, as an active agent contains 2-methyl-5-hexafluoropropoxyfuran, and as a surfactant - sodium salts of secondary alkyl sulfates, as water - distilled water and additionally isopropyl alcohol in the following ratio of components, wt.%:
2-Methyl-5-hexafluoroisopropoxyfuran - 10.8-12.4
Sodium salts of secondary alkyl sulfates - 17.4-21.5
Isopropyl alcohol GOST 9805-84 - 3.0-5.0
Distilled water GOST 6709-72 - Else
A disinfectant is known ("Vilagin", RF patent N 1522498, IPC A 61 L 2/16 of 03/09/94, publ. BI N 25, 1997) 2-methyl-5-hexafluoroisopropoxyfuran. The disadvantages of this tool is its low solubility in water. Therefore, in practice, it has not found application as a disinfectant. In addition, it is oxidized during prolonged storage (more than 3 months) in the light, reducing its activity.

 The introduction of less than the lower limit (10.8) into the formulation of Vilagin does not provide the specified bactericidal activity of the working solution (after dilution). The introduction of Vilagin above the upper limit (12.4) creates excessive bactericidal activity. The introduction of the Progress detergent (sodium salts of secondary alkyl sulfates) into the formulation less than the lower limit (17.4) does not ensure the stability of the product and reduces its washing ability.

 The introduction of a detergent above the upper limit (21.5) does not increase the washing ability, but increases foaming, which creates inconvenience when used.

 The introduction of isopropyl alcohol below the lower limit (3.0) does not reduce foam formation.

 The introduction of isopropyl alcohol above the upper limit (5.0) reduces the detergency of the product.

 In the patent and scientific literature, the applicant has not found a disinfectant with highly effective (biological and detergent) properties, which includes Vilagin, the Progress detergent and isopropyl alcohol. Therefore, this technical solution is "new" with an "inventive step".

 The components of the disinfectant are affordable and cheap, produced by domestic industry, therefore, the proposed composition is "industrially possible and appropriate."

 The invention is illustrated by the following examples.

 Example 1. Preparation of the Givsal disinfectant.

 At room temperature, the Progress detergent was added to the calculated amount of water, then a solution of Vilagin in isopropyl alcohol was loaded. Stir until a homogeneous solution.

 Examples 2-5 are prepared analogously to example 1. The number of components included in the composition are shown in table 1.

 Table 1 shows examples (options) of the Givsal disinfectant formulations. Examples (options) 1, 5 are outside the claimed ranges of component values. Examples (options) 2, 3, 4 are in the claimed ranges of component values.

 Example 6. Disinfectant properties. The study of the disinfecting properties of the drug was carried out according to generally accepted methods: instructions for determining the bactericidal properties of new disinfectants N 739-63, 71 in the modification of the Scientific Research Institute of Disinfectology (Moscow), using conditionally pathogenic microorganisms: E. coli, 1257; Staphylococcus aureus, 906; spores of anthracoid, 1312; Pseudomonas aeruginosa, 165; Proteus, 5/26-III; Micrococcus, ATCC 4698; fecal streptococcus; Candida Albicans, 264/624; tubercle bacillus; polio virus type 1 LSc2AB.

Meat and peptone agar was used to cultivate microorganisms and conduct experiments. The daily test cultures were evenly distributed on the surface of the model test surfaces — tile, enamel, linoleum, metal. Test surfaces were treated with a pre-prepared working solution of the agent until completely moistened. After certain exposures (a single treatment of 30 minutes), the surface was wiped with sterile wipes (5x5), which were then immersed in sterile water, washed, and washed were seeded in Petri dishes with MPA. Petri dishes with crops were placed for 24-48 hours in a thermostat at 37 ^o C. The results were calculated and the percentage of disinfection was determined. The results are presented in table 2.

 From table 2 it is seen that the treatment with Givsal disinfectant provides disinfection of test surfaces made of tiles, enamel, linoleum, metal, infected with Escherichia coli and Staphylococcus aureus from 99.99% to 100%, in the claimed range of values, options 2- 4 at an exposure of 30 minutes

 A decrease in Vilagin, Progress detergent and isopropyl alcohol below the lower limit leads to a decrease in the percentage of disinfection.

 An increase in the amount of Vilagin, Progress detergent and isopropyl alcohol above the upper limit does not lead to an increase in the percentage of disinfection, therefore it is impractical. In addition, due to the increase in the surfactant, the foaming of the agent increases, which creates inconvenience during operation.

 The fact that Vilagin is a less dangerous active ingredient than peracetic acid is proved by comparing their safety characteristics presented in Table 3.

 Thus, the goal is achieved of creating a disinfectant, including a less aggressive component, low risk in the manufacturing and use of the product, which has a high effect and stability.

 The fact that the proposed disinfectant retains microbiological activity for a long time follows from table 4.

Product Safety Feature:
Disinfectant ("Givsal") is a class III drug of moderately hazardous substances, with a single contact in its native form it has a weak irritating effect on the skin, does not have a sensitizing effect.

 Example 7. Method of application.

 The disinfectant ("Givsal") before use is diluted with water to a working concentration in the ratio of wt. parts from 1:20 (0.5%) to 1: 4 (3%). They are treated with a surface solution, medical devices, equipment, dental instruments (Table 5).

 The virucidal activity of the Givsal preparation was studied in accordance with the Methodological Recommendations for the determination of the virucidal activity of drugs (M., N 1110-73, approved by the Ministry of Health of the USSR September 6, 73).

 A violated strain of polio virus type 1 LSc2AB served as a model of viral contamination. In a container with sterile tap water containing 10-100 virions in 1 ml, the test concentrations of Givsal were added (1.0%; 2.0%; 5.0%; 10.0%; 50.0%), thoroughly mixed and put in contact. Givsal was not added to the control tank. To assess the dynamics of poliomyelitis virus inactivation in water under the influence of various Givsala concentrations, to determine the threshold and minimum effective concentration of water samples for the presence of the virus, we took from each container after 6, 12, 24, 48, 72 hours. The concentration of poliomyelitis virus in the initial and research samples was determined by the method of plaque forming units (PFU / ml) in accordance with the Guidelines for the sanitary-virological control of environmental protection (M., 1982) in flat bottles on primary trypsinized tissue culture (kidney cells of green monkeys BS -C-1). The experimental data are summarized and summarized in tables 1, 2. Judging by the results, the Givsal preparation has a virucidal effect against poliomyelitis virus type 1 LSc2AB in concentrations: 2% - threshold, 5% - minimum effective concentration, follows from Table. 6-7.

The anti-tuberculosis effect of the Givsal disinfectant was studied in a specialized licensed laboratory of the Perm Regional Center of Phthisiopulmonology. Tests were carried out on model surfaces (enamel, linoleum) both in the presence of protein load and without it (Instructions for determining the bactericidal properties of new disinfectants N 739-68). The monthly culture of mycobacterium tuberculosis isolated from patients was washed with sterile distilled water at a rate of 1: 1 and adjusted to 5 units according to the turbidity standard. The resulting suspension infected test surfaces on the eve of the experiment. The next day, infected test objects were immersed in test concentrations. Through certain exposures, surfaces were removed from Givsal disinfectant containers, surfaces were wiped with tissues that were immersed in a 10% neutralizer solution. After 10 minutes of shaking, crops were sown on nutrient media of Levinshtein - Jensen and Mordovsky. Crops were incubated in a thermostat at a temperature of 37 ^o C for 90 days. The results presented in the table show 100% death of mycobacterium tuberculosis after a 1-hour exposure of 3 and 5% concentration of the drug "Givsal".

Identification of the disinfecting effect of the impact of 0.5-1.0% Givsal solutions on dental instruments [general and surgical (tweezers, mirrors, scissors, scalpels); rotating (tools for the root canal, burs, cutters); orthopedic (spatulas, elevators, ironing machines) in laboratory conditions showed 100% disinfection of instruments infected with vegetative forms of bacteria, after 30-60 minutes of action of 0.5% Givsala solutions. Instruments contaminated with Candida albicans culture were disinfected with 2% solutions at a temperature of 20 ^o C for 30 minutes. 100% disinfection was observed when exposed to 2% working solutions (50 ^o C) for 15-20 minutes.

Practical tests of the Givsal disinfectant in 2 dental clinics in Perm, conducted with the aim of evaluating the effectiveness of disinfecting surgical instruments, evaluating the effectiveness of the drug for surface treatment of equipment, presented in Table 7, and a comparative evaluation of 2% Givsal solution and 3% chloramine solution showed that the optimal regimen of the drug is 2% concentration of the working solution at a temperature of 18 ^o C and a holding time of 1 hour. The Givsal preparation is not inferior to a 3% solution of chloramine in terms of the degree of disinfecting effect and at the same time has several advantages: it does not leave traces after processing, does not require repeated washing off of product residues from surfaces and wiping with a dry cloth, is convenient to use, and does not have a pronounced unpleasant odorless, does not irritate the skin of the hands, does not have an allergenic effect.

Claims (1)

A disinfectant containing an active additive, a surfactant and water, characterized in that it contains 2-methyl-5-hexafluoroisopropoxyfuran as an active additive, and sodium salts of secondary alkyl sulfates as a surfactant, and distilled as water water and additionally isopropyl alcohol in the following ratio of components, wt.%:
2-Methyl-5-hexafluoro-isopropoxyfuran - 10.8 - 12.4
Sodium salts of secondary alkyl sulfates - 17.4 - 21.5
Isopropyl alcohol - 3.0 - 5.0
Distilled Water - Else

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