RU2146874C1 - Food yeast-based biologically-active food additive - Google Patents

Abstract

FIELD: food industry. SUBSTANCE: additive contains selenium-containing yeast subjected to hydroacoustic treatment and autolysis. Autolysate contains at least 50% of peptide fraction with molecular weight 3 to 24 kDa. Additive possesses hypoallergenic activity and increases capacity for work. EFFECT: extended assortment food yeast-based biologically- active additives. 4 ex

Description

 The invention relates to the food industry, namely to biologically active additives (BAA) obtained from food yeast.

 It is known to use dried autolysates of food baking yeast as a restorative, supplying amino acids and vitamins dietary supplements, which nonetheless has a relatively low nutritional value [1].

 The closest to the proposed food supplement is a dietary supplement, obtained from the autolysate of food baking yeast [2], which has the property of correction and general strengthening effect [3].

 The technical results of the claimed invention is to obtain a dietary supplement with hypoallergenic and performance enhancing properties, as well as expanding the range of dietary supplements from food yeast.

 This is achieved through the use of edible selenium-containing yeast, which is subjected to hydroacoustic treatment and subsequent autolysis until at least 50% of the peptide fraction with a molecular weight in the range of 3-24 kDa is reached in the dietary supplement.

 The objective of the invention is to obtain a product with a hypoallergenic effect, as well as new biological properties.

 The experiments showed that the product with the desired properties can be obtained only from selenium-containing yeast, which were subjected to hydroacoustic treatment, which allows to increase the bioavailability of selenium and its degree of digestibility.

 Previously, selenium-containing yeast was used in the prevention of cardiovascular diseases, but the use of an autolysate of selenium-containing yeast significantly expands the range of biological effects, while a significantly lower amount of dietary supplements is required to achieve a positive effect.

 A positive effect is achieved by using new raw materials, and sonar processing with subsequent autolysis allows you to get a product with the maximum number of biologically active peptides.

 Below are examples of the preparation of this product, as well as experimental data confirming the new properties of the obtained dietary supplement.

Example 1. Dry selenium-containing yeast is dissolved in water to obtain yeast milk and with a solids concentration (dry matter) of 16%. The suspension is processed in a sonar unit and subjected to further autolysis in a thermostat at a temperature of 50 ^o C. In the resulting autolysate, the composition of the peptide fraction is characterized by the molecular weight distribution shown in table. 1.

Example 2. The same as example 1, but dry yeast was taken 200 g, the concentration of CB in the resulting yeast milk is 18%. The suspension is processed in a sonar unit and subjected to further autolysis in a thermostat at a temperature of 55 ^o C for 16 hours. In the resulting autolysate, the composition of the peptide fraction is characterized by the molecular weight distribution shown in table. 2.

 The resulting product is a friable powder, well preserving its properties for 12 months.

 The products of examples 1 and 2 to determine their biological activity were tested in mice and rats.

 Example 3. The antianaphylactic (hypoallergenic) effect of the drug obtained according to example 1 was evaluated in experiments on laboratory animals (rats) using a model of systemic anaphylaxis. The experiment was carried out on male Wistar rats weighing 220-300 g.

 Rats were divided into four groups of 10 animals each.

 The animals of the first experimental group were sensitized intraperitoneally with chicken ovalbumin (OVA) and, starting from the first day of sensitization, they were additionally administered intragastrically for 28 days with a probe of 120 mg prototype yeast autolysate and 60 mg of the product of Example 1 (6.0 μg of selenium per rat per day) in 2.0 ml of saline.

 Animals of the second experimental group sensitized OVA similarly to animals of the first experimental group. In addition to the vivar diet, they received intragastrically 180 mg of the prototype yeast autolysate in 2.0 ml of physiological saline.

 Animals of the third experimental group sensitized OVA in the same way as animals of the first and second groups. They received only a vivar diet.

The animals of the fourth (control) group were not sensitized and kept on a vivar diet, daily introducing intragastrically 2.0 ml of physiological saline. Sensitization was carried out according to the following scheme: on the first, third, and fifth day, 100 mg of OVA with Al ₂ (OH) ₃ were administered intraperitoneally to the rats, and on the twenty-first day, 10.0 mg of OVA with Al ₂ (OH) ₃ was booster.

 On the twenty-ninth day, a resolution dose of 0.70 mg OVA in 0.5 ml of an isotonic sodium chloride solution was administered intravenously to sensitized animals. The severity of the manifestation of anaphylactic shock was assessed by the state of the intestinal barrier, determined by the absorption of the indicator of intestinal permeability - polyethylene glycol with a molecular weight of 4000 daltons (PEG-4000). The absorption of PEG-4000 was determined in turn by its urinary excretion, which was analyzed by gel permeation chromatography on a Toyopearl HW-50 column [4]. At the same time, the concentration of creatinine was determined colorimetrically in urine samples [5]. Absorption of PEG-4000 was expressed in% per 1 mmol of excreted creatinine. The results of this determination are as follows: taking Vitasil reduced the severity of anaphylaxis compared with sensitized animals of the third group by 25%. Reception "Vitasila-Se" showed a normalizing effect on the state of the protective barrier of the small intestine of sensitized animals, significantly reducing their severity of anaphylactic shock by 40%. The action of this drug was so pronounced that the determined indicator does not differ from the corresponding indicator in intact animals (control group). The efficiency of utilization by animals of selenium, which is part of Vitasila-Se, is indicated by an increase in its content in the blood serum and erythrocytes of animals of the first group.

 Thus, the product of example 1 is well absorbed by sensitized animals and has an antianaphylactic (hypoallergenic) effect.

Example 4. The effect on adaptation to physical activity of the preparation of example 2 was investigated in experiments on mice. CBA mice were divided into two groups of 25 animals each. Animals of the experimental group for 6 days were additionally obtained by intragastric administration (probe) of 3 mg Vitasila-Se and 20 mg Vitasila in 0.1 ml of physiological saline against a standard diet. Animals of the control group were on a standard vivar diet. On the seventh day, the performance of animals of both groups was evaluated by testing the duration of their swimming (test "Swimming time before drowning") at a water temperature of 16 ^o C. The results of the study, in which the time to drowning animals of the first group is on average one and a half times longer than the second, indicate a significant, statistically significant increase in physical endurance in mice that received Vitasil-Se as dietary supplements.

List of references
1. Patent of Russia N 2003262, cl. A 23 J 1/18; A 61 K 35/66; A 61 K 35/78
2. Patent of Russia N 20875531, 1997 - prototype.

 3. Materials of the scientific conference "New Information Technologies in Medicine and Ecology". Ukraine, Crimea, Yalta-Gurzuf, May 1997

 4. Gmoshinsky IV, Verina T.Yu., Mazo VK, Morozov IA, Bulletin of the experimental biol. and honey., 1992, t. 114, N 11, p. 532-534.

 5. Biological research methods in the clinic (edited by A. Pokrovsky).

Claims (1)

 A biologically active food supplement based on food yeast containing an autolysate of food yeast, characterized in that selenium-containing yeast subjected to hydroacoustic processing is used as food yeast, the autolysate of which contains at least 50% of the peptide fraction with a molecular weight in the range of 3 - 24 kDa.

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