RU2127596C1 - Method of increase of sexual behavior activity in experiment - Google Patents

Abstract

FIELD: biology, medicine, pharmacology. SUBSTANCE: method involves the use of 7-10 wt.-% an aqueous infusion of knot-grass herb (Polygonum avicularis) at the dose 4.5-5.5 ml/kg rat weight for 8-10 days. Administration of the indicated preparation enhances an activity of sexual behavior. EFFECT: enhanced effectiveness of an agent, decreased cost.

Description

 The invention relates to the field of pharmacology, and in particular to methods and substances that increase the activity of sexual behavior, and can be used as an aphrodiatic in experimental practice.

 The emerging tendency to a decrease in libido and potency in both men and women has recently been a major problem of modern sexology, which has social significance. The search for means to stimulate ethological sexual reactions is highly relevant.

 Closest to the claimed technical solution is a natural psychotropic drug pantocrine, used as a tonic and representing a tincture of the non-ossified horns of red deer or sika deer on ethanol [Mashkovsky M.D. Pantocrine / Medicines. Part 1. -Medicine, 1987.-p.142]. The drug has gonadotropic activity and, as a result, increases libido [Yu. I. Dobryakov Antlers. -Vladivostok: SB Far Eastern Scientific Center, Academy of Sciences of the USSR, 1970.-32 p .; Dobryakov Yu.I., Brekhman I.I. The biological activity of antlers of various species of deer / Medicines of the Far East. Issue 11. - Vladivostok, 1972.-p. 197-201]. However, the effectiveness of the drug as a stimulant of sexual behavior is insufficient. The drug is contraindicated in organic diseases of the heart, angina pectoris, nephritis, and others. The stocks of raw materials for pantocrine production are limited, for example, in the Primorsky Territory a herd of sika deer stag is only about 15,000 heads, they require care and maintenance costs; the cost of antlers is very high and currently amounts to about 30,000 rubles per kg, in addition, the production of pantocrine tincture requires high-quality deficient ethyl alcohol.

 Known grass (knotweed) of the mountaineer bird (Herba Polygoni avicularis) of the buckwheat family (Polygonaсeae) [D. Muravyova / Pharmacology.-M.: Medicine, 1981. -58 p. ], the infusions of which are used as astringent and vitamin-containing drugs, as well as for certain diseases: malaria, tuberculosis, gastric diseases, whooping cough, etc. [Pashinsky V.G. Plants in the treatment and prevention of diseases. -Tomsk: Tomsk University, 1989-208 p.].

The objective of the invention is a cheap affordable tool with the ability to increase the activity of sexual behavior. The problem is achieved by applying the water extract of the grass of the mountaineer avian following composition, wt.%:
Dry Highlander Herbs - 7 - 10
Distilled Water - Else
administered orally in doses of 4.5 to 5.5 mg / kg for 8 to 9 days.

 The use of the mountaineer bird as a stimulant of sexual behavior was not previously known.

 The inventive infusion is prepared in a standard way [Infusions and decoctions / State Pharmacopoeia of the USSR. Eleventh Edition. Issue 2. General methods of analysis. Medicinal plant material. -M.: Medicine, 1990.-p. 147].

 Example 1. Prepared 6 infusions with a mass fraction of grass of the mountaineer bird according to table 1. The experiments were conducted on four-month-old sexually mature (body weight 160-180 g) male white outbred rats, which were divided into 7 groups. For 8 days, once a day at a dose of 5 ml / kg, water (control) was injected into the animals of the 1st group using a probe, infusion with a mass fraction of highlander grass was administered to animals of the first group: 5 wt.% To animals of the 2nd group %, 3rd - 6 wt.%, 4th - 7 wt.%, 5th - 10 wt.%, 6th - 12 wt.% And 7th 15 wt.%, Then the males were planted in the females and observed pair behavior within 10 minutes The activity of sexual behavior was determined by [Buresh Y., Bureshova O., Houston J.P. Methods and basic experiments to study the brain and behavior. -M .: Higher school, 1991. -300 pp.], The criterion was the number of components of sexual behavior: sniffing, licking, cage, ejaculation and the total number of components. The results of the experiments are presented in table 1.

 A significant difference between experience and control is noted with a minimum share of the highlander in the infusion of 7 wt%, with a share of the highlander in the infusion of more than 10 wt%, the number of components of sexual behavior, i.e. the activity of sexual behavior remains virtually unchanged. Thus, it is possible to take the following highlander contents in the infusion in wt%: lower - 7, upper - 0.

 Example 2. Cooked 2 infusions with a share of grass of the mountaineer bird 7 and 10 wt.%. The experiments were conducted on four-month-old mature (body weight 160-180 g) male white outbred rats. For 8 days once a day, one animal was injected with water using a probe (control) for one animal, and for another animal (experiment), the infusion of the mountaineer grass in doses according to Tables 2 and 3. Then, the males were planted with the females and sexual activity was determined similarly to example 1 The results of the experiments are presented in tables 2 and 3.

 A significant difference between experience and control according to the criteria for sexual behavior is noted at a minimum dose of 5.5 ml / kg with a content of highlander grass in the infusion equal to 7 wt%, with a further increase in dose having little effect on sexual activity. When the share of the mountaineer in the infusion is 10 wt%, the number of components of sexual behavior, i.e. the activity of sexual behavior is significantly different at a minimum dose of 4.5 ml / kg, a further increase in the dose has little effect on sexual activity. Thus, the following dose limits can be taken when the highlander content in the infusion is 7-10 wt%: lower - 4.5 ml / kg, upper - 5.5 ml / kg.

 Example 3. Prepared 2 infusions with a share of grass of the mountaineer bird 7 and 10 wt.%. The experiments were conducted on 4-month-old mature (body weight 160-180 g) male white outbred rats. For 4-12 days, once an animal was injected once a day with a probe to one animal, water (control), to another animal (experiment), infusion of the mountaineer bird in doses of 4.5 and 5.5 ml / kg. Then the males were planted with the females and the activity of sexual behavior was determined analogously to example 1. The results of the experiments are presented in table 4.

 A significant difference between experience and control according to the criteria for sexual behavior is noted at a minimum dose of 4.5 ml / kg with a content of highlander grass in the infusion equal to 7 wt.% When administered for 10 days, with a further increase in the duration of administration, it has little effect on sexual activity. When the share of the mountaineer in the infusion is 7 wt.%, The number of components of sexual behavior, i.e. the activity of sexual behavior is significantly different from the control at a dose of 5.5 ml / kg on the 9th day of administration, a further increase in the duration of administration has little effect on sexual activity. When the content of highlander grass in the infusion is 10 wt.% And doses of 4.5 and 5.5 ml / kg, a significant difference between sexual behavior and control is noted on the 8th and 9th days, respectively, and with a further increase in the timing of administration, it does not change much. Thus, the minimum duration of the introduction of infusion of mountaineer grass with its mass fraction in the infusion of 7-10 wt% and a dose of 4.5-5.5 ml / kg is 8-12 days.

 Example 4. Comparison of the infusion of grass of the mountaineer bird with the famous pantocrine (analogue), Prepared 2 infusions with a share of grass of the mountaineer of 7 and 10 wt.%. The experiments were conducted on 4-month-old mature (body weight 160-180 g) male white outbred rats. For 8-10 days once a day, pantocrine was administered orally using a probe to one animal according to [Dobryakov Yu.I., Brekhman II. The biological activity of antlers of various species of deer / Medicines of the Far East. Vol. 11. - Vladivostok: FEB Academy of Sciences of the USSR, 1972. -p.197-201] in doses of 0.1 - the recommended therapeutic dose and 1 ml / kg (control), for other animals (experience) infusion of highlander in doses of 4.5 and 5.5 ml / kg. Then the males were seated to the males and the activity of sexual behavior was determined analogously to example 1. The results of the experiments are shown in table 5.

 From table 5 it is seen that the activity of sexual behavior in animals receiving infusion of mountaineer in doses of 4.5-5.5 ml / kg for 8-10 days with a share of grass in the infusion of 7-10 wt.%, Higher in 1, 5-2 times compared with animals that received pantocrine at a dose of 0.1 ml / kg for 8-10 days. An increase in the dose of pantocrine to 1 ml / kg (10 times higher than the recommended therapeutic dose) leads to a slight increase in the activity of sexual behavior, but it is still lower than in animals treated with the infusion of highlander in the above doses.

 Example 5. Comparison of the cost of a course dose of pantocrine and mountaineer bird with comparable biological effects. Calculation without taking into account the cost of production of drugs, but only taking into account the cost of the main raw materials: antlers, alcohol and grass of the mountaineer; approximate calculations, their results are summarized in table 6.

 Pantocrine. Dose 1 ml / kg for 8-10 days.

To prepare 1000 ml of the drug, 0.1 kg of dry horns and 0.5 kg of ethyl alcohol are required. 1 kg of horns costs 30,000 rubles., 1 kg of ethyl alcohol is about 100 rubles. ; 0.1 kg of horns - 3000 rubles and 0.5 kg of alcohol - 50 rubles; total cost of the main raw materials for the preparation of 1000 ml of pantocrine is:
3000 + 50 = 3050 rubles, and for 1 ml - 3.05 rubles.

At a dose of 1 ml / kg per animal (body weight about 200 g), 0.2 ml is consumed per administration, and for administration within 8-10 days, 1.6 - 2 ml are required, respectively, cost:
1.6 • 3.05 = 4.8 rubles, 2 • 3.05 = 6.1 rubles.

 Infusion of the mountaineer. The dose is 4.5 ml / kg, the mass fraction of grass in the infusion is 7 wt.%, For 8 to 10 days.

1 kg of grass costs 100 rubles. To prepare 1000 ml of infusion, 0.07 kg of grass is required, which costs respectively:
0.07 • 100 = 7 rubles, therefore, for 1 ml - 0.007 rubles.

 At a dose of 4.5 ml / kg for one administration, an animal (weighing about 200 g) needs 0.9 ml or for 8-10 days from 7.2 to 9 ml.

Cost:
7.2 • 0.007 = 0.0504 rubles; 9 • 0.007 = 0.063 rubles.

 Similar calculations were made with other dosages and the content of the herb in the infusion (see table 6).

 As can be seen from table 6, the cost of a course dose of infusion of highlander bird grass to increase sexual activity is 40 to 120 times cheaper with a more pronounced effect compared to the analogue of pantocrine. The reserves of grass of the mountaineer are practically unlimited [Wulf EV, Maleeva O.F. Polugonum L. - Highlander / World stocks of useful plants.- L.: Nauka, 1969 .-- p. 138-139], the range is very wide, the grass is a wild plant does not require agricultural technology, care, the cost of only collection and processing.

Claims (1)

 A method of increasing the activity of sexual behavior in an experiment by administering a drug, characterized in that 7 - 10 wt.% Water extract of the mountaineer bird grass at doses of 4.5 - 5.5 ml / kg of rat weight is used as a medicine for 8 - 10 days.

Images