RU2097765C1 - Method for diagnosing malignant neoplasms - Google Patents

Abstract

FIELD: medicine. SUBSTANCE: method involves examining blood serum. Three drops of blood serum are placed on the microscope slide, introducing 0.05-01% alpha-aminoglutariic acid solution into every drop, covering with microscope slides, holding for 1-2 h at 35-38 C, carrying out microscopic examination in polarized light. Long or short branching dendrite crystals, radial crystals or their combination being detected in the preparation, the cases of malignant neoplasms are diagnosed. EFFECT: simplified method. 9 dwg

Description

 The invention relates to medicine and biology and can be used for rapid diagnosis of preclinical stages of tumor diseases.

 A known method for the diagnosis of malignant neoplasms using chemiluminescence of blood serum. Ultra-weak luminescence of blood serum (SC) in patients with malignant tumors is reduced in comparison with the luminescence of SC of healthy individuals (Zhuravlev A.I. Zhuravleva A.I. . The SK of the test person was placed in a cylindrical cell made of cross with an optical bottom, followed by examination in a special installation, which consists of photomultiplier units, an object, power, and registration. An FEU-42 photomultiplier was used. The recording of signals was displayed on the screen of the recording device. Manually using the cursor, the spectrogram was scanned for the maximum amplitudes and the corresponding wavelengths of excitation and emission. Then the spectrogram was decoded.

The disadvantages of the method:
the duration of the method (at least one day), the complexity of the implementation, the availability of expensive equipment, the need for a large amount of blood serum. In addition, diagnosis is possible only at the clinical stages of malignant neoplasms.

 The invention is aimed at solving the problem of diagnosing preclinical stages of tumor diseases, accelerating and simplifying the method.

 This task is achieved by conducting a crystalloscopic study of the sample in polarized light (for example, using a Min-8 polarizing microscope) prepared from drops of the blood serum of a suspect with drops of 0.05 0.1% glutamic acid introduced into them.

Then drops of SC of the suspect (3-6) separately, with a volume of 0.02 0.03 ml each, are applied to a glass slide, 0.01 ml (one drop) of 0.05 0.1% glutamic acid solution is introduced into each drop , covered with coverslips, dried at T 35-38 ^o C in a dry-air oven for 1-2 hours, then kept in the open air for 1 2 hours and conduct crystalloscopic (optical) research in polarized light. If the sample contains crystals of the following forms: radial beam, short- and long-branched dendrites or their combinations, a malignant neoplasm is diagnosed.

 The course of biochemical processes at the preclinical stages of the development of proliferative (malignant) conditions is accompanied by an increased content of glutamic acid in the blood serum of a sick person. It is necessary as plastic material for tumor growth (Shapot V.S. Biochemical aspects of tumor growth. M. 1975, Blinov V.A. Biochemical manifestations of the systemic effect of the tumor on the body (nitrogen, carbohydrate metabolism, agluconeogenesis). Thesis. Doctor. Kiev, 1975). 0.05-0.1% glutamic acid solution was used with the aim of its possible crystallization in serum.

 The placement of a sample drop between two glasses (oriented crystallization) leads to the appearance of specific crystals of dendritic microforms characteristic of glutamic acid under conditions of its "supersaturation" of the study medium.

The temperature of 35 38 ^o C contributes to the manifestation of the optical properties of the sample without significant violations of its biological structure.

 A drying time of 1-2 hours is selected for maximum moisture removal.

 Holding the drug in the open air for 1-2 hours enhances crystal growth.

The method is as follows:
The blood serum of the test person separately in the form of drops (3-6), volume 0.02 0.03 ml each, is applied to slides, 0.01 ml (one drop) of 0.05 0.1% solution is introduced into each drop glutamic acid, covered with coverslips and dried in a dry-air oven for 1 2 hours at T 35 38 ^o C, then kept in the open air for 1-2 hours and examined in polarized light and in the presence of radial beam, or short or long-branched dendritic crystals or their combinations determine a malignant neoplasm.

 The results of the proposed method are presented in 9 figures, which illustrate variants of dendritic microforms obtained from SK patients suspected of having a neoplasm in the early stages of their development.

 Used blood serum of patients with blood diseases (lymphocytic leukemia, myelogenous leukemia), tumors of the nervous system (neurofibroma), tumors of the mammary gland, uterus and appendages.

 In FIG. 1 shows a sample prepared from SC of a healthy person with glutamic acid introduced into it. There are no dendritic crystals; in FIG. 2 in the sample of the suspected person there are radial beam dendritic crystals. First detected chronic lymphocytic leukemia; in FIG. 3 in the sample there are radial beam dendritic crystals. Chronic myeloid leukemia was detected for the first time; in FIG. 4 long-branched dendrites are present in the sample of the suspect Breast neoplasms were first detected; in FIG. 5 long-branched dendritic crystals. First detected breast cancer; in FIG. 6, the sample contains radial beam and long-branched dendritic crystals. The cancer of the appendages was first detected; in FIG. 7 long branched dendrites are present in the sample. The cancer of the appendages was first detected; in FIG. 8, short-branched dendritic crystals are present in the sample. First discovered neurofibroma; in FIG. 9 in the sample of the suspected person there are radial beam and short-branched dendritic crystals.

 Uterine tumor.

 Example 1. FIG. 1, SC of a healthy person with 0.1% glutamic acid solution introduced into it. There are whiskers that respond weakly to polarized light. Glutamic acid level is 52 m / mol micromol.

Technology: three drops of SC of a healthy person were applied to a glass slide, each with a volume of 0.02 ml, 0.1 ml of a 0.1% solution of glutamic acid was introduced into each drop, the drops were covered with coverslips and dried for 1 h in a dry-air cabinet at T 37 ^o C. The resulting preparation was allowed to stand in the open air for 1 h, and then microscopied in polarized light.

 Example 2. A sample prepared from the SC of a suspect. Radial beam dendritic crystals are present. Glutamic acid content is 80 micro / mol. Suspicion of a tumor process. The diagnosis was confirmed. Chronic lymphocytic leukemia was first detected.

Technology: four drops of SC of a suspected person, each 0.03 ml in volume, were applied to a glass slide, 0.1 ml of 0.1% glutamic acid solution was introduced into each drop, the drops were covered with coverslips and dried for 2 hours in a dry air cabinet at T 37 ^o C. The resulting preparation was allowed to stand in the open air for 2 hours, and then microscopied in polarized light.

 Example 3. A sample prepared from the SC of the suspect. Radial beam dendritic crystals are present. Glutamic acid content is 75 micro / mol. Suspicion of a neoplasm. The diagnosis was confirmed: chronic myeloid leukemia was first detected.

Technology: three drops of SC of a suspected person, each 0.02 ml in volume, were applied to a glass slide, 0.1 ml of a 0.1% glutamic acid solution was introduced into each drop, the drops were covered with coverslips and dried for 1 h in a dry air cabinet at T 35 ^o C. The resulting preparation was kept in the open air for 2 hours, then microscopically in polarized light.

 Example 4. FIG. 4. A sample prepared from the SC of the suspect. Long-branched dendritic crystals are present. Glutamic acid content 68 micro / mol. Suspicion of a neoplasm. The diagnosis was confirmed. Breast neoplasm.

Technology: three drops of a suspect's SC were applied to a glass slide, each 0.02 ml in volume, 0.1 ml of a 0.05% glutamic acid solution was introduced into each drop, the drops were covered with coverslips and dried for 1 hour in an air-dry cabinet for an hour at T 36 ^o C. The drug was then kept in the open air for 1 h, and then microscopied in polarized light.

 Example 5. FIG. 5. A sample prepared from the SC of the suspect. Long-branched dendritic crystals are present. Glutamic acid content is 74 micro / mol. Suspicion of a neoplasm. The diagnosis was confirmed. Mammary cancer.

Technology: drops of SC of a suspected person are applied to a glass slide, 0.1% glutamic acid solution is introduced dropwise into each and covered with coverslips, then dried in a dry-air cabinet at T 38 ^o C for 1 h, kept in the open air for 1 h and microscope in polarized light.

 Example 6. FIG. 6. A sample prepared from the SC of the suspect. There are radial beam and long-branched crystals. The cancer of the appendages was first detected.

Technology: drops of SC of the test person are applied four on a glass slide with a volume of 0.02 ml each, a 0.1% solution of glutamic acid (0.01 ml) is introduced into the drop, covered with coverslips and dried in a dry air cabinet at T 37 ^o C for 2 hours, the resulting preparation is kept in the open air, and then microscopied in polarized light.

 Example 7. FIG. 7. The sample contains long-branched dendrites. The cancer of the appendages was first detected.

Technology: drops of SC of the test person are applied to a glass slide with a volume of 0.03 ml each, 0.01 ml of a 0.1% glutamic acid solution is introduced into the drop, the drops are covered with glass slides, dried in an thermostat at T 37 ^o C, then kept on open air for 1 h and microscope in polarized light.

 Example 8. FIG. 8. In the sample prepared from the SC of the suspect, there are short-branched dendritic crystals. First found neurofibroma.

Technology: three drops of SC of the test person are applied to a glass slide, each with a volume of 0.02 ml, then a drop of 0.1% glutamic acid solution is introduced into each drop, the drops are covered with coverslips and dried in an thermostat at T 38 ^o C for 1 h, the resulting preparation is kept in the open air for 1 h, and then microscopied in polarized light.

 Example 9. FIG. 9. In the sample of the suspect, there are radial beam and short-branched dendritic crystals. The diagnosis was confirmed. Uterine tumor.

Technology: six drops of SC of a suspected person with a volume of 0.03 ml each are applied to a glass slide, then one drop of a 0.1% glutamic acid solution is introduced into the drops, the drops are covered with coverslips and dried in a dry air cabinet at T 38 ^o C for 1 h. The resulting preparation is kept in the open air for 2 hours and microscopic in polarized light.

 Positive effect: speed of use of the method, accessibility, the ability to identify preclinical stages of malignant neoplasms.

Claims (1)

A method for the diagnosis of malignant neoplasms by examining blood serum, characterized in that drops of blood serum are applied to a glass slide, 0.05 0.1% glutamic acid solution is injected into each drop, covered with coverslips, dried at 35–38 ^° C for 1 2 hours, kept in the open air for 1 2 hours, then a crystalloscopic examination is carried out in polarized light and if the preparation contains radial beam or short or long-branched dendritic crystals, or combinations thereof, they are diagnosed with malignant quality neoplasm.

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