RU2035877C1 - Antioxidant for stabilization of vitamins in feed mixtures - Google Patents

Abstract

FIELD: fodder production. SUBSTANCE: solution of vitamin A is treated with antioxidant constituted by 2-hydroxy-3.5-bis-tret-amylophenin-2H-benztriasole. EFFECT: better nutrient properties. 2 tbl

Description

 The invention relates to the stabilization of vitamins in premixes and animal feed.

 Known methods for stabilizing vitamins in feed mixtures involving the introduction of antioxidants, for example, butyloxytoluene (2,6-di-tert-butylparacresol), diludine (2,6-dimethyl-3,5-dicarboxy-1,4-dihydropyridine) (Dvinskaya L .M. And Shubin A.A. Use of antioxidants in animal husbandry (L. Agropromizdat, 1986, p. 12-13), the addition of which to feed mixtures helps to improve their quality by preserving vitamins in their composition.

 However, the limited solubility of, for example, diludine in oils requires its use in feed mixtures with a filler. Small doses of butyloxytoluene and diludine do not have a stabilizing effect on vitamins, and in large doses they lead to the accumulation of phenolic compounds in the animal’s body, which leads to undesirable consequences.

 The closest effect achieved is Santoquin (6-hydroxy-2,2,4-trimethyl-1,2-dihydroquinoline) (Dvinskaya L.M. and Shubin A.A. Use of antioxidants in animal husbandry. L. Agropromizdat, 1986, p. . 12), having a high inhibitory activity, due to which the conservation of vitamin A in feed is achieved.

However, according to GOST 12.1.007-76, Santokhin is a substance of the 3rd hazard class: the average lethal dose (LD ₅₀ ) with a single oral administration for mice and rats is 1730-3000 mg / kg (Piulskaya V.I. et al. Study of the toxicity of antioxidant Santokhin, used to stabilize feed products. Proceedings of the All-Russian Research Institute of Meat Industry, issue 36, M. 1976, pp. 59-63).

 The disadvantage of Santokhin is also that it has difficult storage conditions (low temperature, tightness), and a limited shelf life (9 months). The use of Santokhin is difficult, since it is a sedentary, viscous liquid, poorly mixed in feed mixtures.

 The aim of the invention is to expand the assortment of antioxidants that protect against the decomposition of vitamins in feed mixtures during storage, less toxic compared to known antioxidants, are more economically effective.

 This goal is achieved by using 2-hydroxy-3,5-bis-tert-amylphenyl-2H-benzotriazole for the first time as an antioxidant for vitamin A.

 2-Hydroxy-3,5-bis-tert-amylphenyl-2H-benzotriazole is a known substance of the series of hydroxyphenylbenzotriazoles (Chemical additives to polymers. Reference / Edited by Maslova I.P. Chemistry, 1981), usually used as a light stabilizer for polypropylene and polyurethane. Its use as an antioxidant of vitamins was not previously known.

 2-Hydroxy-3,5-bis-tert-amylphenyl-2H-benzotriazole due to the presence of intramolecular hydrogen bonds reduces the content of peroxide radicals in feed mixtures, coming into contact with oxygen-containing free radicals of vitamins, stabilizes them. This protects vitamins from decomposition, dramatically increasing the stability of feed additives. Therefore, the proposed technical solution meets the criterion of "novelty", since the use of this substance to stabilize vitamins is proposed for the first time.

The present invention meets the criterion of "significant differences", since, firstly, the proposed antioxidant is effective in doses half as much as generally accepted; secondly, according to GOST 12.1.007-76, this substance belongs to the 4th hazard class, since the maximum dose (15000 mg / kg) administered orally to white mice did not cause a fatal outcome, while LD ₅₀ Santokhin for mice 1730 mg / kg; thirdly, in application, the proposed powdery antioxidant is more convenient than a viscous liquid Santokhin (prototype); fourthly, the high stability of 2-hydroxy-3,5-bis-tert-amylphenyl-2H-benzotriazole does not limit the shelf life of the drug under strict observance of storage rules, while for the prototype the shelf life is 9 months.

2-Hydroxy-3,5-bis-tert-amylphenyl-2H-benzotriazole was tested as an antioxidant of vitamins in laboratory conditions in comparison with the well-known prototype Santokhin (TU 6-02-1288-84). Laboratory tests were carried out on pure solutions of vitamin A in oil according to a known method (Waldman A.R. et al. Izvestia AN SSSR 5, 1973). A portion of the antioxidant was dissolved in 15 ml of a working solution of vitamin A, containing 510 IU / ml in the 1st experiment, and 1060 IU / ml in the 2nd experiment. After stirring the solution allowed to stand for 5 and 10 days at ⁷⁰ ° C Acid number mg KOH / g of oil solutions investigated and vitamin A activity was determined in IU TU 6425-126-75.

 These tests are shown in the tables: the drug with an initial concentration of vitamin A 510 IU / ml in the table. 1, with a concentration of 1060 IU / ml in table. 2.

The value of the relative errors in determining the acid number and activity of vitamin A were respectively not more than 0.04 and 3%
The data table. 1 and 2 indicate that the proposed antioxidant in comparison with the prototype provides a significant preservation of vitamin A in time due to a significant decrease in acid number, which allows to increase the period of use of high-quality biologically active feed additives.

 Preservation of vitamin A and acid number in a comparative study of the activity of antioxidants.

Using the invention will allow:
to increase the stability of vitamin A in pure solutions, premixes and compound feeds, thereby extending the term of use of the latter;
expand the range of non-toxic domestic preparations for livestock vitamin stabilizers;
will provide an opportunity to increase the estimated economic effect by 2 times compared with the use of the prototype by reducing the dose by 2 times when a positive effect is achieved.

Claims (1)

 The use of 2-hydroxy-3,5-bis-tert-amylphenin-2H-benzotriazole as an antioxidant to stabilize vitamins in feed mixtures.

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