RU2021126356A - A method of creating tissue-engineered constructions by the method of bioprinting with bio-ink for the regeneration of tissues and organs in the conditions of an organism - Google Patents

A method of creating tissue-engineered constructions by the method of bioprinting with bio-ink for the regeneration of tissues and organs in the conditions of an organism Download PDF

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RU2021126356A
RU2021126356A RU2021126356A RU2021126356A RU2021126356A RU 2021126356 A RU2021126356 A RU 2021126356A RU 2021126356 A RU2021126356 A RU 2021126356A RU 2021126356 A RU2021126356 A RU 2021126356A RU 2021126356 A RU2021126356 A RU 2021126356A
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Russia
Prior art keywords
bio
ink
extracellular matrix
bioprinting
regeneration
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RU2021126356A
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Russian (ru)
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RU2021126356A3 (en
RU2770558C2 (en
Inventor
Евгений Евгеньевич Бекетов
Елена Васильевна Исаева
Надежда Валерьевна Аргучинская
Анастас Андреевич Кисель
Анна Николаевна Филимонова
Григорий Александрович Демяшкин
Петр Викторович Шегай
Андрей Дмитриевич Каприн
Original Assignee
Федеральное государственное бюджетное учреждение «Национальный медицинский исследовательский центр радиологии» Министерства здравоохранения Российской Федерации (ФГБУ «НМИЦ радиологии» Минздрава России)
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Priority to RU2021126356A priority Critical patent/RU2770558C2/en
Publication of RU2021126356A publication Critical patent/RU2021126356A/en
Publication of RU2021126356A3 publication Critical patent/RU2021126356A3/ru
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Publication of RU2770558C2 publication Critical patent/RU2770558C2/en

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/14—Macromolecular materials
    • A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
    • A61L27/24—Collagen
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/52—Hydrogels or hydrocolloids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/56—Porous materials, e.g. foams or sponges
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Medicinal Chemistry (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Dermatology (AREA)
  • Transplantation (AREA)
  • Epidemiology (AREA)
  • Dispersion Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Organic Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physical Education & Sports Medicine (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Materials For Medical Uses (AREA)

Claims (1)

Способ создания тканеинженерных конструкций методом биопечати биочернилами для регенерации тканей и органов в условиях организма, включающий приготовление биочернил, которые получают непосредственно перед процессом 3D-биопечати, и далее используют для создания тканеинженерной конструкции, отличающийся тем, что биочернила включают в себя следующие компоненты: коллаген, мезенхимальные стволовые клетки (МСК) и порошок децеллюляризованного внеклеточного матрикса (дВКМ), причем дВКМ получали из реберных хрящей самок крыс линии Wistar, которые подвергали децеллюляризации и лиофилизации, полученный порошок просеивают через сито с диаметром пор 280 мкм и до использования хранят при +4°С, при приготовлении биочернил с концентрацией коллагена 4% используют стерильный ателоколлаген свиньи I типа – 80 мг/мл, который смешивают с МСК – 5×106 в мл, с буферным раствором – 250 мкл на мл и порошком дВКМ – 25 мг/мл, далее скаффолд печатают и заливают теплой питательной средой температурой 37°C, после чего проводят процесс инкубации при 37°C в CO2-инкубаторе.A method for creating tissue-engineered structures by bio-printing using bio-ink for the regeneration of tissues and organs under the conditions of the body, including the preparation of bio-ink, which is obtained immediately before the process of 3D-bioprinting, and then used to create a tissue-engineered structure, characterized in that the bio-ink includes the following components: collagen, mesenchymal stem cells (MSCs) and decellularized extracellular matrix (decellularized extracellular matrix) powder, whereby the decellularized extracellular matrix (decellularized extracellular matrix) was obtained from the rib cartilage of female Wistar rats, which were decellularized and lyophilized, the resulting powder was sieved through a sieve with a pore diameter of 280 μm and stored at + 4 ° until use C, when preparing bio-ink with a collagen concentration of 4%, sterile type I pig atelocollagen is used - 80 mg / ml, which is mixed with MSC - 5 × 10 6 in ml, with a buffer solution - 250 μl per ml and dVKM powder - 25 mg / ml , then the scaffold is printed and filled with a warm nutrient medium with a temperature of 37 ° C, after then the incubation process is carried out at 37 ° C in a CO 2 incubator.
RU2021126356A 2021-09-07 2021-09-07 Method for creating tissue-engineering structures by bioprinting with bioink for cartilage tissue regeneration under body conditions RU2770558C2 (en)

Priority Applications (1)

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RU2021126356A RU2770558C2 (en) 2021-09-07 2021-09-07 Method for creating tissue-engineering structures by bioprinting with bioink for cartilage tissue regeneration under body conditions

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Application Number Priority Date Filing Date Title
RU2021126356A RU2770558C2 (en) 2021-09-07 2021-09-07 Method for creating tissue-engineering structures by bioprinting with bioink for cartilage tissue regeneration under body conditions

Publications (3)

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RU2021126356A true RU2021126356A (en) 2021-10-27
RU2021126356A3 RU2021126356A3 (en) 2022-02-22
RU2770558C2 RU2770558C2 (en) 2022-04-18

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114940971A (en) * 2022-06-24 2022-08-26 中山大学 Method for constructing high-activity cartilage tissue based on Faraday wave

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EA028039B1 (en) * 2014-09-05 2017-09-29 Частное Учреждение Лаборатория Биотехнологических Исследований "3Д Биопринтинг Солюшенс" Device and methods for printing biological tissues and organs
EP3771547A1 (en) * 2019-08-02 2021-02-03 Technische Universität Berlin Method for 3d printing of vascularized tissues and organs

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114940971A (en) * 2022-06-24 2022-08-26 中山大学 Method for constructing high-activity cartilage tissue based on Faraday wave
CN114940971B (en) * 2022-06-24 2024-03-15 中山大学 Construction method of high-activity cartilage tissue based on Faraday wave

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RU2021126356A3 (en) 2022-02-22
RU2770558C2 (en) 2022-04-18

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