RU2018146175A - METHOD OF MOLECULAR MARKING BASED ON MICROSATELITE LOCUS, INTENDED FOR GENETIC PASPORTIZATION OF SELECTIVE ACHIEVEMENTS OF PLANTS OF THE GENUS RUBUS - Google Patents

METHOD OF MOLECULAR MARKING BASED ON MICROSATELITE LOCUS, INTENDED FOR GENETIC PASPORTIZATION OF SELECTIVE ACHIEVEMENTS OF PLANTS OF THE GENUS RUBUS Download PDF

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RU2018146175A
RU2018146175A RU2018146175A RU2018146175A RU2018146175A RU 2018146175 A RU2018146175 A RU 2018146175A RU 2018146175 A RU2018146175 A RU 2018146175A RU 2018146175 A RU2018146175 A RU 2018146175A RU 2018146175 A RU2018146175 A RU 2018146175A
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plants
dna
pasportization
microsatelite
achievements
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RU2018146175A
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RU2732922C2 (en
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Елена Олеговна Видягина
Наталья Михайловна Субботина
Вадим Георгиевич Лебедев
Константин Александрович Шестибратов
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Федеральное государственное бюджетное образовательное учреждение высшего образования "Пущинский государственный естественно-научный институт" (ПущГЕНИ)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
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Claims (4)

1. Способ молекулярно-генетической паспортизации сортов малины, ежевики и малино-ежевичных гибридов на основе микросателлитных (SSR) маркеров, заключающийся в выделении ДНК из исследуемых образцов, проведении ПЦР, электрофоретическом разделении продуктов амплификации ДНК, документировании результатов ПЦР с помощью системы гель-документации, определении длин амплифицированных фрагментов ДНК путем сравнения со стандартами молекулярной массы, получении таблицы выявления среди продуктов амплификации присущих только для данного сорта профилей по микросателлитным локусам, отличающийся тем, что амплификацию ДНК проводят по следующему набору микросателлитных локусов с использованием фланкирующих их праймеров:1. The method of molecular genetic certification of varieties of raspberries, blackberries and raspberry-blackberry hybrids based on microsatellite (SSR) markers, which consists in isolating DNA from test samples, performing PCR, electrophoretic separation of DNA amplification products, documenting the results of PCR using a gel documentation system , determining the lengths of amplified DNA fragments by comparison with molecular weight standards, obtaining a identification table among the amplification products of profiles specific for a given variety of microsatellite loci, characterized in that DNA amplification is carried out according to the following set of microsatellite loci using flanking primers:
Figure 00000001
Figure 00000001
Figure 00000002
Figure 00000002
 2. Способ по п. 1, отличающийся тем, что выделение ДНК осуществляют из материала, взятого от не менее десяти растений каждого сорта, выращенных в поле или защищенном грунте или не менее двадцати растений каждого сорта, выращенных в условиях in vitro.2. The method according to p. 1, characterized in that the extraction of DNA is carried out from material taken from at least ten plants of each cultivar grown in a field or sheltered ground or at least twenty plants of each cultivar grown in vitro.
RU2018146175A 2018-12-25 2018-12-25 Method for molecular labelling, based on microsatellite loci, intended for genetic certification of plant variety of rubus genus plants RU2732922C2 (en)

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RU2388828C1 (en) * 2008-10-28 2010-05-10 Государственное научное учреждение Всероссийский научно-исследовательский институт масличных культур имени В.С. Пустовойта Российской академии сельскохозяйственных наук Method for identification of soya breeds on basis of microsatellite (ssr) markers
RU2620967C2 (en) * 2015-02-17 2017-05-30 Общество с ограниченной ответственностью Научно-производственное предприятие "МИКРОКЛОН" (ООО НПП "МИКРОКЛОН") Method of identifying raspberry varieties based on rapd-markers
RU2017128334A (en) * 2017-08-09 2019-02-11 Елена Олеговна Видягина Microsatellite locus-based molecular labeling method for genetic certification of raspberry, blackberry and raspberry-blackberry hybrid selection achievements

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