RU2017128334A - Microsatellite locus-based molecular labeling method for genetic certification of raspberry, blackberry and raspberry-blackberry hybrid selection achievements - Google Patents

Microsatellite locus-based molecular labeling method for genetic certification of raspberry, blackberry and raspberry-blackberry hybrid selection achievements Download PDF

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RU2017128334A
RU2017128334A RU2017128334A RU2017128334A RU2017128334A RU 2017128334 A RU2017128334 A RU 2017128334A RU 2017128334 A RU2017128334 A RU 2017128334A RU 2017128334 A RU2017128334 A RU 2017128334A RU 2017128334 A RU2017128334 A RU 2017128334A
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raspberry
blackberry
dna
achievements
labeling method
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RU2017128334A
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Russian (ru)
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Елена Олеговна Видягина
Наталья Михайловна Субботина
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Елена Олеговна Видягина
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1. Способ молекулярно-генетической паспортизации сортов малины, ежевики и малино-ежевичных гибридов на основе микросателлитных (SSR) маркеров, заключающийся в выделении ДНК из исследуемых образцов, проведении ПЦР, электрофоретическом разделении продуктов амплификации ДНК, документировании результатов ПЦР с помощью системы гель-документации, определении длин амплифицированных фрагментов ДНК путем сравнения со стандартами молекулярной массы, получении таблицы выявления среди продуктов амплификации присущих только для данного сорта профилей по микросателлитным локусам, отличающийся тем, что амплификацию ДНК проводят по следующему набору микросателлитных локусов с использованием фланкирующих их праймеров:1. The method of molecular genetic certification of raspberry, blackberry and raspberry-blackberry hybrids based on microsatellite (SSR) markers, which consists in isolating DNA from test samples, performing PCR, electrophoretic separation of DNA amplification products, documenting the results of PCR using a gel documentation system , determining the lengths of amplified DNA fragments by comparison with molecular weight standards, obtaining a identification table among the amplification products inherent only for this variety sirloin of microsatellite loci, wherein the DNA amplification is performed on the next set of microsatellite loci with their flanking primers:
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Figure 00000002
 2. Способ по п. 1, отличающийся тем, что выделение ДНК осуществляют из материала, взятого от не менее десяти растений каждого сорта, выращенных в поле или защищенном грунте или не менее двадцати растений каждого сорта, выращенных в условиях in vitro.2. The method according to p. 1, characterized in that the extraction of DNA is carried out from material taken from at least ten plants of each cultivar grown in a field or sheltered ground or at least twenty plants of each cultivar grown in vitro.
RU2017128334A 2017-08-09 2017-08-09 Microsatellite locus-based molecular labeling method for genetic certification of raspberry, blackberry and raspberry-blackberry hybrid selection achievements RU2017128334A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2732922C2 (en) * 2018-12-25 2020-09-24 Федеральное государственное бюджетное образовательное учреждение высшего образования "Пущинский государственный естественно-научный институт" (ПущГЕНИ) Method for molecular labelling, based on microsatellite loci, intended for genetic certification of plant variety of rubus genus plants

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2732922C2 (en) * 2018-12-25 2020-09-24 Федеральное государственное бюджетное образовательное учреждение высшего образования "Пущинский государственный естественно-научный институт" (ПущГЕНИ) Method for molecular labelling, based on microsatellite loci, intended for genetic certification of plant variety of rubus genus plants

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