RU2018144685A - Method for producing alpha-fetoprotein immobilized on PET microparticles - Google Patents

Method for producing alpha-fetoprotein immobilized on PET microparticles Download PDF

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Publication number
RU2018144685A
RU2018144685A RU2018144685A RU2018144685A RU2018144685A RU 2018144685 A RU2018144685 A RU 2018144685A RU 2018144685 A RU2018144685 A RU 2018144685A RU 2018144685 A RU2018144685 A RU 2018144685A RU 2018144685 A RU2018144685 A RU 2018144685A
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Russia
Prior art keywords
microparticles
fetoprotein
alpha
polyethylene terephthalate
pet
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RU2018144685A
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Russian (ru)
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RU2018144685A3 (en
RU2731415C2 (en
Inventor
Виктория Евгеньевна Кузнецова
Ринат Азатович Мифтахов
Сергей Анатольевич Лапа
Максим Анатольевич Спицын
Валерий Евгеньевич Шершов
Теймур Октаевич Гусейнов
Эдуард Николаевич Тимофеев
Сергей Павлович Радько
Андрей Валерьевич Лисица
Александр Васильевич Чудинов
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Общество с ограниченной ответственностью "ИБМХ-ЭкоБиоФарм" (ООО "ИБМХ-ЭкоБиоФарм")
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Priority to RU2018144685A priority Critical patent/RU2731415C2/en
Publication of RU2018144685A3 publication Critical patent/RU2018144685A3/ru
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Peptides Or Proteins (AREA)

Claims (6)

1. Реагент на основе альфа-фетопротеина, иммобилизованного на твердых микрочастицах полиэтилентерефталата (ПЭТ-микрочастицах).1. A reagent based on alpha-fetoprotein immobilized on solid microparticles of polyethylene terephthalate (PET microparticles). 2. Способ получения реагента по п. 1, заключающийся в иммобилизации альфа-фетопротеина на поверхности ПЭТ-микрочастиц в количестве не менее 100 пикомоль альфа-фетопротеина на 1 мг микрочастиц полиэтилентерефталата.2. A method of producing a reagent according to claim 1, which consists in immobilizing alpha-fetoprotein on the surface of PET microparticles in an amount of not less than 100 picomoles of alpha-fetoprotein per 1 mg of microparticles of polyethylene terephthalate. 3. Способ по п. 2, отличающийся тем, что иммобилизация альфа-фетопротеина на поверхности твердых микрочастиц полиэтилентерефталата производится сайт-специфически за гликозидный фрагмент белка.3. The method according to p. 2, characterized in that the immobilization of alpha-fetoprotein on the surface of solid microparticles of polyethylene terephthalate is site-specific for the glycosidic fragment of the protein. 4. Способ по п. 2, отличающийся тем, что связывание альфа-фетопротеина с поверхностью твердых микрочастиц полиэтилентерефталата происходит через гидрофильный спейсер протяженностью 13 атомов.4. The method according to p. 2, characterized in that the binding of alpha-fetoprotein to the surface of solid microparticles of polyethylene terephthalate occurs through a 13-atom hydrophilic spacer. 5. Способ по п. 2, отличающийся тем, что на поверхности микрочастиц полиэтилентерефталата щелочным гидролизом формируются реактивные карбоксильные групы, которые пептидной связью связываются со спейсером, несущим концевую аминогруппу.5. The method according to claim 2, characterized in that reactive carboxyl groups are formed on the surface of microparticles of polyethylene terephthalate by alkaline hydrolysis, which are bound by a peptide bond to a spacer carrying an amino end group. 6. Способ по п. 2, отличающийся тем, что модифицированные микрочастицы полиэтилентерефталата через аминогруппу спейсера связываются с альдегидными группами гликозидного фрагмента альфа-фетопротеина прочной ковалентной связью.6. The method according to p. 2, characterized in that the modified microparticles of polyethylene terephthalate through the amino group of the spacer bind to the aldehyde groups of the glycoside fragment of alpha-fetoprotein strong covalent bond.
RU2018144685A 2018-12-17 2018-12-17 Method of producing alpha-fetoprotein immobilized on pet microparticles RU2731415C2 (en)

Priority Applications (1)

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RU2018144685A RU2731415C2 (en) 2018-12-17 2018-12-17 Method of producing alpha-fetoprotein immobilized on pet microparticles

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RU2018144685A RU2731415C2 (en) 2018-12-17 2018-12-17 Method of producing alpha-fetoprotein immobilized on pet microparticles

Publications (3)

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RU2018144685A3 RU2018144685A3 (en) 2020-06-17
RU2018144685A true RU2018144685A (en) 2020-06-17
RU2731415C2 RU2731415C2 (en) 2020-09-02

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Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2420594C2 (en) * 2009-05-20 2011-06-10 Наталья Николаевна Угарова Agent for adenosine-5'-triphosphate
KR101384746B1 (en) * 2011-08-24 2014-04-14 포항공과대학교 산학협력단 Surface immobilization of various functional biomolecules using mussel adhesive protein
US9778265B2 (en) * 2015-04-16 2017-10-03 The United States Of America, As Represented By The Secretary Of The Navy Charged peptide appendage to facilitate oriented protein covalent immobilization

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RU2018144685A3 (en) 2020-06-17
RU2731415C2 (en) 2020-09-02

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