RU2017486C1 - Method of complex bacterial preparation preparing - Google Patents

Method of complex bacterial preparation preparing Download PDF

Info

Publication number
RU2017486C1
RU2017486C1 SU5016276/14A SU5016276A RU2017486C1 RU 2017486 C1 RU2017486 C1 RU 2017486C1 SU 5016276/14 A SU5016276/14 A SU 5016276/14A SU 5016276 A SU5016276 A SU 5016276A RU 2017486 C1 RU2017486 C1 RU 2017486C1
Authority
RU
Russia
Prior art keywords
bacterial preparation
preparation
preparation preparing
complex bacterial
microbial cells
Prior art date
Application number
SU5016276/14A
Other languages
Russian (ru)
Inventor
А.В. Григорьев
В.А. Знаменский
В.Н. Клевцов
Original Assignee
Григорьев Александр Вячеславович
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Григорьев Александр Вячеславович filed Critical Григорьев Александр Вячеславович
Priority to SU5016276/14A priority Critical patent/RU2017486C1/en
Application granted granted Critical
Publication of RU2017486C1 publication Critical patent/RU2017486C1/en

Links

Images

Classifications

    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

FIELD: medicine. SUBSTANCE: for preparing of medicinal biopreparation charcoal is used (particle size is less 30 microns). Microbial cells are applied on surface of biopreparation at surface concentration 2.1·103 - 1.0·105. Use of proposed preparation will ensure to enhance efficacy of disbiose correction. EFFECT: enhanced quality of preparation. 2 tbl

Description

Изобретение относится к медицине. Целью изобретения является повышение качества. The invention relates to medicine. The aim of the invention is to improve the quality.

Способ осуществляется следующим образом. The method is as follows.

Порошковидный уголь, состоящий из частиц размером до 30 мкм, смешивают с микробными клетками аутохтонных или вакцинных штаммов микроорганизмов в соотношении 0,1 угля и 1,0˙109 - 3,0˙109 клеток. Полученную смесь выдерживают при 4-10оС в течение 20-40 мин при постоянной вибрации. Далее смесь разбавляют физиологическим раствором хлористого натрия (0,9%) в соотношении 1: 1, переносят в емкости, в которых полученный биопрепарат подвергают лиофильной сушке, соответствующей режиму сушки бактерийных препаратов.Powdered coal, consisting of particles of size up to 30 microns, is mixed with microbial cells or vaccine strains of autochthonous microorganisms in the ratio of coal and 0.1 1,0˙10 9 - 3,0˙10 9 cells. The resulting mixture was kept at 4-10 ° C for 20-40 minutes at constant vibration. Next, the mixture is diluted with physiological sodium chloride solution (0.9%) in a 1: 1 ratio, transferred to containers in which the resulting biological product is subjected to freeze drying, corresponding to the drying regime of bacterial preparations.

П р и м е р 1. Обоснование антиинфекционной активности. PRI me R 1. The rationale for anti-infective activity.

У белых крыс воспроизводят сальмонелезную инфекцию путем одноразового введения сальмонелл. На третий день после заражения и в течение двух последующих дней четырем группам животных вводят комплексные и коммерческие биопрепараты, содержащие пограничные значения поверхностных концентраций (2,1˙103 и 1,0˙105) микробных клеток на 1 мм2 угля. Одна группа зараженных животных является контрольной.In white rats, salmonella infection is reproduced by a single administration of salmonella. On the third day after infection and over the next two days, four groups of animals are injected with complex and commercial biological products containing boundary values of surface concentrations (2.1 310 3 and 1.0˙10 5 ) of microbial cells per 1 mm 2 of coal. One group of infected animals is the control.

Проводят высев гомогената слизистых на питательные среды и подсчитывают количество выросших сальмонелл. Результаты приведены в табл. 1. Mucosal homogenate is plated on culture media and the number of salmonella grown is counted. The results are shown in table. 1.

П р и м е р 2. Обоснование уровня приживляемости. PRI me R 2. Justification of the level of engraftment.

Определяют уровень приживляемости аутохтонных микроорганизмов в кишечнике у здоровых людей при введении комплексных биопрепаратов, включающих уголь с частицами менее 30 мкм и более 30 мкм (30-50) и микробные клетки с пограничным значением поверхностных концентраций (2,1˙103 и 1,0˙105). В качестве контроля используют коммерческие бактерийные препараты, вводимые в аналогичных объемных концентрациях (1,0˙109 и 3,0˙109). На протяжении 5 сут двукратно проводят посев кала для выявления количества вводимых микроорганизмов. Предварительно у всех волонтеров проводят исследование кала на содержание нормальной микрофлоры кишечника.The level of engraftment of autochthonous microorganisms in the intestines in healthy people is determined with the introduction of complex biological products, including coal with particles less than 30 μm and more than 30 μm (30-50) and microbial cells with a boundary value of surface concentrations (2.1˙10 3 and 1.0 ˙10 5 ). As a control, commercial bacterial preparations are used, administered in similar volume concentrations (1.0 × 10 9 and 3.0 × 10 9 ). For 5 days, feces are sown twice to determine the number of introduced microorganisms. Previously, all volunteers carry out a study of feces for the content of normal intestinal microflora.

Результаты приведены в табл. 2. The results are shown in table. 2.

Использование изобретения позволит повысить эффективность коррекции дисбиоза. The use of the invention will improve the correction of dysbiosis.

Claims (1)

СПОСОБ ПОЛУЧЕНИЯ КОМПЛЕКСНОГО БАКТЕРИЙНОГО ПРЕПАРАТА аухтонного штамма путем соединения микроорганизма с основой, отличающийся тем, что, с целью повышения качества препарата, в качестве основы используют активированный уголь, поверхность частиц которого соединяют с микробными клетками в концентрации на 1 мм2 2,1 · 103 - 1,0 · 105, при этом активированный уголь используют в виде порошка с размером частиц менее 30 мкм.METHOD FOR PRODUCING AN INTEGRATED BACTERIA PREPARATION for the aukhton strain by combining a microorganism with a base, characterized in that, in order to improve the quality of the preparation, activated carbon is used as a base, the particle surface of which is connected to microbial cells at a concentration of 1 mm 2 2.1 · 10 3 - 1.0 · 10 5 , while activated carbon is used in the form of a powder with a particle size of less than 30 microns.
SU5016276/14A 1991-12-02 1991-12-02 Method of complex bacterial preparation preparing RU2017486C1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
SU5016276/14A RU2017486C1 (en) 1991-12-02 1991-12-02 Method of complex bacterial preparation preparing

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SU5016276/14A RU2017486C1 (en) 1991-12-02 1991-12-02 Method of complex bacterial preparation preparing

Publications (1)

Publication Number Publication Date
RU2017486C1 true RU2017486C1 (en) 1994-08-15

Family

ID=21591415

Family Applications (1)

Application Number Title Priority Date Filing Date
SU5016276/14A RU2017486C1 (en) 1991-12-02 1991-12-02 Method of complex bacterial preparation preparing

Country Status (1)

Country Link
RU (1) RU2017486C1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2740140C1 (en) * 2020-07-13 2021-01-11 Общество с ограниченной ответственностью "АВАН" Method of producing and a composition for increasing the effectiveness of probiotic microorganisms

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Руководство по вакцинно-сывороточному делу, Л., 1978, с.150. *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2740140C1 (en) * 2020-07-13 2021-01-11 Общество с ограниченной ответственностью "АВАН" Method of producing and a composition for increasing the effectiveness of probiotic microorganisms

Similar Documents

Publication Publication Date Title
DE60035670T2 (en) USE OF LACTOBACILLUS SALIVARIUS
DE69226622T2 (en) Diet or drug compositions containing lyophilized bacteria
JP4410992B2 (en) Bacterial strains, treated plant extracts, compositions containing them, methods for their preparation, and their therapeutic and industrial applications
KR20050083758A (en) Composition exhibiting stabilised oxidation-reduction properties and method for the stabilisation thereof
WO1996038137A1 (en) Methods for maintaining or elevating the cd-4 or cd-8 cell levels comprising the administration of conjugated linoleic acid; a new process for the manufacture of conjugated linoleic acid using lactobacillus sp
WO2007058027A1 (en) Harmful bacterium control agent containing bacillus thuringiensis
EP1102580A2 (en) Formulations having probiotically active microorganisms
DE3781652T2 (en) PREPARATION SUITABLE FOR TREATMENT OF COLONAL DISEASES.
EA006847B1 (en) Solid composition containing bacillus-type non-pathogenic bacterial spores
CN1177636A (en) Bacterial stain of bacillus subtilis and application thereof
CA1179265A (en) Bifidobacteria-proliferating agent
RU2017486C1 (en) Method of complex bacterial preparation preparing
RU2203946C1 (en) Strain of bacterium lactobacillus acidophilus nv ep 317/402-x "balans-narine" used for preparing antigastritis and antiulcerous fermented-milk product
CA1311204C (en) Growth promoter for bifidobacterium species, process for preparing the same and method of using the same
JPH02503800A (en) Bacterial preparations for the prevention and treatment of inflammatory processes and allergic diseases
SU1487815A3 (en) Method of producing biologically active substance having immunostimulating effect
CN114432227A (en) Oral care solution containing probiotics
CN1177637A (en) Preparation of living bacillus subtilis and preparation method thereof
DE3716938A1 (en) Pharmaceutical composition for the treatment of colic in infants
JPH01502340A (en) Preparations for the prevention and treatment of gastrointestinal diseases in livestock
US5929109A (en) Enhancing and stabilizing agent of the activity of Bifidus factor
SU1722502A1 (en) Medicinal preparation ъbiosporinъ for prevention and treatment of gastrointestinal diseases in man
JPS5839624A (en) Antitumor agent
RU2262530C2 (en) Method preparing dried preparation based on bifido- and/or lactobacillus and preparation prepared by this method
KR102606636B1 (en) Bacillus velezensis having the effect of preventing or improving for sarcopenia and uses thereof

Legal Events

Date Code Title Description
QZ4A Changes in the licence of a patent

Effective date: 19951101

QZ4A Changes in the licence of a patent

Effective date: 19951101