RU2016150216A - DRUG FOR BIODEGRADATION OF OIL PRODUCTS AND METHOD FOR PRODUCING IT - Google Patents

DRUG FOR BIODEGRADATION OF OIL PRODUCTS AND METHOD FOR PRODUCING IT Download PDF

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Publication number
RU2016150216A
RU2016150216A RU2016150216A RU2016150216A RU2016150216A RU 2016150216 A RU2016150216 A RU 2016150216A RU 2016150216 A RU2016150216 A RU 2016150216A RU 2016150216 A RU2016150216 A RU 2016150216A RU 2016150216 A RU2016150216 A RU 2016150216A
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RU
Russia
Prior art keywords
vkpm
association
bacillus megaterium
bacteria
cells
Prior art date
Application number
RU2016150216A
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Russian (ru)
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RU2016150216A3 (en
RU2681831C2 (en
Inventor
Михаил Юрьевич Волков
Рустам Маратович Абдуллин
Сергей Владимирович Аникин
Дмитрий Александрович Венков
Зульфар Салихович Салихов
Original Assignee
Акционерное общество "Альфахимпром"
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Application filed by Акционерное общество "Альфахимпром" filed Critical Акционерное общество "Альфахимпром"
Priority to RU2016150216A priority Critical patent/RU2681831C2/en
Publication of RU2016150216A publication Critical patent/RU2016150216A/en
Publication of RU2016150216A3 publication Critical patent/RU2016150216A3/ru
Application granted granted Critical
Publication of RU2681831C2 publication Critical patent/RU2681831C2/en

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
    • B09C—RECLAMATION OF CONTAMINATED SOIL
    • B09C1/00—Reclamation of contaminated soil
    • B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales

Claims (6)

1. Препарат для биодеградации нефтепродуктов, содержащий ассоциацию бактерий, включающую в себя штаммы бактерий Bacillus subtilis ВКПМ В-5328, Pseudomonas putida ВКПМ В-5624, Rhodococcus erythropolis ВКПМ АС-1269 и штамм Bacillus megaterium, иммобилизированную на глауконитсодержащем носителее, отличающийся тем, что он в качестве штамма Bacillus megaterium содержит микроорганизмы штамма Bacillus megaterium ВКПМ В-607,1. A preparation for biodegradation of petroleum products containing an association of bacteria, including the bacterial strains Bacillus subtilis VKPM B-5328, Pseudomonas putida VKPM B-5624, Rhodococcus erythropolis VKPM AC-1269 and the Bacillus megaterium strain, which is immobilized, is slightly greener. it as a strain of Bacillus megaterium contains microorganisms of the strain Bacillus megaterium VKPM B-607, 2. Препарат по п. 1, отличающийся тем, что он содержит ассоциацию бактерий в концентрации 107-1010 клеток/г2. The drug according to p. 1, characterized in that it contains an association of bacteria in a concentration of 10 7 -10 10 cells / g 3. Препарат по п. 1, отличающийся тем, что содержание глауконита в сорбенте составляет не менее 60% масс.3. The drug under item 1, characterized in that the content of glauconite in the sorbent is at least 60% of the mass. 4. Препарат по п. 1, отличающийся тем, что доля клеток отдельных видов бактерий в ассоциации составляет: Bacillus megaterium ВКПМ В-607 - 35-50%, Bacillus subtilis ВКПМ В-5328 - 15-25%, Pseudomonas putida ВКПМ В-5624 - 15-25%, Rhodococcus erythropolis ВКПМ АС-1269 - 15-25%.4. The drug according to claim 1, characterized in that the proportion of cells of certain bacterial species in the association is: Bacillus megaterium VKPM B-607 - 35-50%, Bacillus subtilis VKPM B-5328 - 15-25%, Pseudomonas putida VKPM B- 5624 - 15-25%, Rhodococcus erythropolis VKPM AC-1269 - 15-25%. 5. Препарат по п. 4, отличающийся тем, что доля клеток отдельных видов бактерий в ассоциации составляет: Bacillus megaterium ВКПМ В-607 - 40%, Bacillus subtilis ВКПМ В-5328 - 20%, Pseudomonas putida ВКПМ В-5624 - 20%, Rhodococcus erythropolis ВКПМ АС-1269 - 20%.5. The drug according to claim 4, characterized in that the proportion of cells of certain types of bacteria in the association is: Bacillus megaterium VKPM B-607 - 40%, Bacillus subtilis VKPM B-5328 - 20%, Pseudomonas putida VKPM B-5624 - 20% , Rhodococcus erythropolis VKPM AC-1269 - 20%. 6. Способ получения препарата для биодеградации нефтепродуктов включающий в себя раздельное культивирование бактерий, приготовление их ассоциации, напыление клеток на носитель обезвоживание смеси путем подачи подогретого до 40°C воздуха, отличающийся тем, что используют фракцию глауконита со средним диаметром частиц 600 мкм, подвергнутую термообработке при температуре 190°C до остаточной влажности 4%, готовят микробную ассоциацию смешиванием культуры Bacillus megaterium ВКПМ В-607, Bacillus subtilis ВКПМ В-5328, Pseudomonas putida ВКПМ В-5624, Rhodococcus erythropolis ВКПМ АС-1269 в соотношении микроорганизмов 2:1:1:1 и обезвоживают при достижении влажности в конечном биопрепарате 4%.6. A method of obtaining a preparation for biodegradation of petroleum products, including separate cultivation of bacteria, preparation of their association, spraying cells onto a carrier, dehydrating the mixture by supplying air heated to 40 ° C, characterized in that a heat-treated fraction of glauconite with an average particle diameter of 600 μm is used at a temperature of 190 ° C to a residual moisture content of 4%, a microbial association is prepared by mixing the cultures of Bacillus megaterium VKPM B-607, Bacillus subtilis VKPM B-5328, Pseudomonas putida VKPM B-5624, Rhodococcus erythropolis VKPM AC-1269 in co equipped with microorganisms 2: 1: 1: 1 and dewatered when the humidity in the final Biopreparat 4%.
RU2016150216A 2016-12-20 2016-12-20 Preparation for biodegradation of petroleum products and method for preparation thereof RU2681831C2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU2016150216A RU2681831C2 (en) 2016-12-20 2016-12-20 Preparation for biodegradation of petroleum products and method for preparation thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU2016150216A RU2681831C2 (en) 2016-12-20 2016-12-20 Preparation for biodegradation of petroleum products and method for preparation thereof

Publications (3)

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RU2016150216A true RU2016150216A (en) 2018-06-21
RU2016150216A3 RU2016150216A3 (en) 2018-12-17
RU2681831C2 RU2681831C2 (en) 2019-03-12

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RU2016150216A RU2681831C2 (en) 2016-12-20 2016-12-20 Preparation for biodegradation of petroleum products and method for preparation thereof

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Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2752312C1 (en) * 2020-12-11 2021-07-26 Публичное акционерное общество "Транснефть" (ПАО "Транснефть") Compound for cleaning the water surface from oil and petroleum product films
RU2763428C1 (en) * 2021-05-27 2021-12-29 Общество с ограниченной ответственностью «Научно-производственное объединение «Волга-Экология» Preparation for biodegradation of oil and oil products (oil destructor)

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2302303C2 (en) * 2002-11-06 2007-07-10 Акционерное общество открытого типа "Биохиммаш" Biological preparation for cleaning environments polluted by crude oil and petroleum products
RU2012126442A (en) * 2012-06-25 2013-12-27 Общество с ограниченной ответственностью "ВЕГА-эко" ACINETOBACTER CALCOACETICUS AND ACINETOBACTER RADIORESISTENS CONSORTIUM OF BACTERIA-OIL DESTRUCTORS FOR CLEANING SOIL AND WASTE WATER FROM OIL AND OIL PRODUCTS
RU2571219C2 (en) * 2013-06-25 2015-12-20 Общество с ограниченной ответственностью "Технопарк" Preparation for biodegradation of petroleum products "bioionit" and method of obtaining thereof

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RU2016150216A3 (en) 2018-12-17
RU2681831C2 (en) 2019-03-12

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Effective date: 20190408

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Effective date: 20200921