RU2016148139A - Композиции и способы получения крахмала с новыми функциями - Google Patents
Композиции и способы получения крахмала с новыми функциями Download PDFInfo
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- RU2016148139A RU2016148139A RU2016148139A RU2016148139A RU2016148139A RU 2016148139 A RU2016148139 A RU 2016148139A RU 2016148139 A RU2016148139 A RU 2016148139A RU 2016148139 A RU2016148139 A RU 2016148139A RU 2016148139 A RU2016148139 A RU 2016148139A
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B30/00—Preparation of starch, degraded or non-chemically modified starch, amylose, or amylopectin
- C08B30/12—Degraded, destructured or non-chemically modified starch, e.g. mechanically, enzymatically or by irradiation; Bleaching of starch
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B30/00—Preparation of starch, degraded or non-chemically modified starch, amylose, or amylopectin
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B30/00—Preparation of starch, degraded or non-chemically modified starch, amylose, or amylopectin
- C08B30/04—Extraction or purification
- C08B30/042—Extraction or purification from cereals or grains
- C08B30/044—Extraction or purification from cereals or grains from corn or maize
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
- A01H5/10—Seeds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
- A01H6/46—Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
- A01H6/4684—Zea mays [maize]
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- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B30/00—Preparation of starch, degraded or non-chemically modified starch, amylose, or amylopectin
- C08B30/12—Degraded, destructured or non-chemically modified starch, e.g. mechanically, enzymatically or by irradiation; Bleaching of starch
- C08B30/18—Dextrin, e.g. yellow canari, white dextrin, amylodextrin or maltodextrin; Methods of depolymerisation, e.g. by irradiation or mechanically
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y204/00—Glycosyltransferases (2.4)
- C12Y204/01—Hexosyltransferases (2.4.1)
- C12Y204/01013—Sucrose synthase (2.4.1.13)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y204/00—Glycosyltransferases (2.4)
- C12Y204/01—Hexosyltransferases (2.4.1)
- C12Y204/01021—Starch synthase (2.4.1.21)
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y204/00—Glycosyltransferases (2.4)
- C12Y204/01—Hexosyltransferases (2.4.1)
- C12Y204/01242—NDP-glucose--starch glucosyltransferase (2.4.1.242)
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- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
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- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Polymers & Plastics (AREA)
- Chemical Kinetics & Catalysis (AREA)
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- General Health & Medical Sciences (AREA)
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- Natural Medicines & Medicinal Plants (AREA)
- Nutrition Science (AREA)
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- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Compositions Of Macromolecular Compounds (AREA)
Claims (15)
1. Крахмал, имеющий:
содержание амилозы от приблизительно 2% мас до приблизительно 20% мас, как измеряют с использованием набора для анализа амилозы/амилопектина; и
температуру клейстеризации водного раствора крахмала, составляющую по меньшей мере на приблизительно 5% больше, чем температура клейстеризации контрольного крахмала, как измеряют с использованием анализа RVA,
где крахмал получают из растения кукурузы waxy-sugary 2, содержащего по меньшей мере один аллель wxS в эндосперме.
2. Крахмал по п.1, дополнительно имеющий изменение энтальпии кристаллизации по меньшей мере на приблизительно 30% больше, чем изменение энтальпии кристаллизации контрольного крахмала, при измерении с использованием дифференциального сканирующего калориметра (DSC).
3. Крахмал по любому из пп. 1-2, дополнительно имеющий распределение размера гранул крахмала, где распределение размера гранул крахмала включает по меньшей мере на приблизительно 40% меньше гранул размером менее 5 мкм по сравнению с долей гранул размером менее 5 мкм в контрольном крахмале, что наблюдают с использованием сканирующего электронного микроскопа.
4. Крахмал по любому из пп. 1-3, где крахмал представляет собой гранулу, демонстрирующую повышенную целостность по сравнению с контрольным крахмалом, что наблюдают с использованием сканирующего электронного микроскопа.
5. Крахмал по любому из пп. 1-4, где крахмал способен выдерживать по меньшей мере три цикла замораживания/размораживания.
6. Крахмал по любому из пп. 1-5, где растение кукурузы waxy-sugary 2 получают посредством перекрестного опыления первого растения кукурузы, имеющего генотип wxwxsu2su2, и второго растения кукурузы, имеющего генотип wxSwxSsu2su2 или генотип wxSwxsu2su2.
7. Крахмал по любому из пп. 1-6, где эндосперм растения кукурузы waxy-sugary 2 имеет одну дозу, две дозы или три дозы аллеля wxS, и эндосперм имеет генотип wxSwxwxsu2su2su2, wxSwxSwxsu2su2su2 или wxSwxSwxSsu2su2su2.
8. Крахмал, экстрагированный из растения кукурузы с двойной мутацией, где растение кукурузы является гомозиготой, рецессивной по гену синтазы крахмала IIa (su2), и гомозиготой или гетерозиготой по мутантному гену гранулосвязанной синтазы крахмала I (GBSSI), где мутантный ген GBSSI имеет меньшую активность GBSSI, чем ген GBSSI (Wx) дикого типа, но более высокую активность GBSSI, чем рецессивный ген GBSSI (wx) с потерей функции.
9. Крахмал по п.8, где мутантный ген GBSSI представляет собой аллель wx-Stonor (wxS).
10. Крахмал по п.8 или 9, где растение получают посредством перекрестного опыления первого растения кукурузы, имеющего генотип wxwxsu2su2, и второго растения кукурузы, имеющего генотип wxSwxSsu2su2 или генотип wxSwxsu2su2.
11. Крахмал по любому из пп. 8-10, где эндосперм растения кукурузы имеет одну дозу, две дозы или три дозы аллеля wxS, где эндосперм имеет генотип wxSwxwxsu2su2su2, wxSwxSwxsu2su2su2 или wxSwxSwxSsu2su2su2.
12. Крахмал по любому из пп. 8-11, где крахмал, продуцируемый в эндосперме растения кукурузы, имеет более высокую температуру клейстеризации по сравнению с крахмалом, продуцируемым в эндосперме растения кукурузы с двойной рецессивной мутацией waxy-sugary-2, имеющего генотип wxwxwxsu2su2su2.
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201615090926A | 2016-04-05 | 2016-04-05 | |
US15/090,926 | 2016-04-05 | ||
US15/135,106 US9988641B2 (en) | 2016-04-05 | 2016-04-21 | Compositions and methods for producing starch with novel functionality |
US15/135,106 | 2016-04-21 |
Publications (3)
Publication Number | Publication Date |
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RU2016148139A true RU2016148139A (ru) | 2018-06-09 |
RU2016148139A3 RU2016148139A3 (ru) | 2020-04-20 |
RU2745568C2 RU2745568C2 (ru) | 2021-03-29 |
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Application Number | Title | Priority Date | Filing Date |
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RU2016148139A RU2745568C2 (ru) | 2016-04-05 | 2016-12-08 | Композиции и способы получения крахмала с новыми функциями |
Country Status (12)
Country | Link |
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US (2) | US9988641B2 (ru) |
EP (1) | EP3228635B1 (ru) |
JP (2) | JP7036537B2 (ru) |
KR (1) | KR20170114912A (ru) |
CN (1) | CN107266595B (ru) |
AU (1) | AU2016265967B2 (ru) |
BR (1) | BR102016028652A2 (ru) |
CA (1) | CA2949564C (ru) |
CL (1) | CL2016003187A1 (ru) |
CO (1) | CO2016005548A1 (ru) |
MX (1) | MX2016016336A (ru) |
RU (1) | RU2745568C2 (ru) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109384854A (zh) * | 2018-09-29 | 2019-02-26 | 安徽华明太合生物工程有限公司 | 一种甘薯淀粉 |
CN111118201B (zh) * | 2020-02-23 | 2020-11-03 | 云南省农业科学院农业环境资源研究所 | 与调控水稻低直链淀粉含量基因du13(t)紧密连锁分子标记及其应用 |
CN111996177B (zh) * | 2020-08-17 | 2022-03-04 | 北京市农林科学院 | 一种玉米waxy基因突变体及其分子标记和应用 |
CN112322617A (zh) * | 2020-11-27 | 2021-02-05 | 中国科学院成都生物研究所 | 一种可鉴定大麦籽粒糯性的kasp分子标记及其应用 |
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KR20080023768A (ko) | 2000-03-30 | 2008-03-14 | 화이트헤드 인스티튜트 포 바이오메디칼 리서치 | Rna 간섭의 rna 서열 특이적인 매개체 |
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- 2016-12-07 KR KR1020160165512A patent/KR20170114912A/ko not_active Application Discontinuation
- 2016-12-08 RU RU2016148139A patent/RU2745568C2/ru active
- 2016-12-09 MX MX2016016336A patent/MX2016016336A/es active IP Right Grant
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Also Published As
Publication number | Publication date |
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RU2745568C2 (ru) | 2021-03-29 |
KR20170114912A (ko) | 2017-10-16 |
EP3228635A1 (en) | 2017-10-11 |
MX2016016336A (es) | 2018-06-08 |
JP2017186511A (ja) | 2017-10-12 |
AU2016265967A1 (en) | 2017-10-19 |
CN107266595A (zh) | 2017-10-20 |
US20170283818A1 (en) | 2017-10-05 |
CN107266595B (zh) | 2021-02-05 |
AU2016265967B2 (en) | 2023-08-10 |
JP7157856B2 (ja) | 2022-10-20 |
CL2016003187A1 (es) | 2017-11-03 |
CA2949564C (en) | 2023-09-26 |
US10669553B2 (en) | 2020-06-02 |
JP7036537B2 (ja) | 2022-03-15 |
JP2021181574A (ja) | 2021-11-25 |
CA2949564A1 (en) | 2017-10-05 |
US9988641B2 (en) | 2018-06-05 |
EP3228635B1 (en) | 2019-03-06 |
BR102016028652A2 (pt) | 2020-03-10 |
US20180327767A1 (en) | 2018-11-15 |
CO2016005548A1 (es) | 2018-06-20 |
RU2016148139A3 (ru) | 2020-04-20 |
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Ketthaisong et al. | Changes in physicochemical properties of waxy corn starches at different stages of harvesting | |
Gilding et al. | Allelic variation at a single gene increases food value in a drought-tolerant staple cereal | |
Lu et al. | Effects of heat stress during grain filling on the structure and thermal properties of waxy maize starch | |
Zhang et al. | Comparison of structural and functional properties of wheat starch under different soil drought conditions | |
Awan et al. | Interaction of maternal environment and allelic differences in seed vigour genes determines seed performance in Brassica oleracea | |
Tappiban et al. | Effects of cassava variety and growth location on starch fine structure and physicochemical properties | |
Lu et al. | Effects of waterlogging after pollination on the physicochemical properties of starch from waxy maize | |
Lu et al. | Effects of high temperature after pollination on physicochemical properties of waxy maize flour during grain development | |
Yamamori | Amylose content and starch properties generated by five variant Wx alleles for granule-bound starch synthase in common wheat (Triticum aestivum L.) | |
Yang et al. | Heat stress at different grain filling stages affects fresh waxy maize grain yield and quality | |
Li et al. | Production of waxy tetraploid wheat (Triticum turgidum durum L.) by EMS mutagenesis | |
Ketthaisong et al. | Physicochemical and morphological properties of starch from fresh waxy corn kernels | |
Agama-Acevedo et al. | Characteristics of maize starch and relationship with its biosynthesis enzymes | |
Lu et al. | An SNP downstream of the OsBEIIb gene is significantly associated with amylose content and viscosity properties in rice (Oryza sativa L.) | |
Vignola et al. | Genotypic and environmental effects on starch properties of Argentinean wheat flours | |
Huo et al. | Effects of the duration of post‐silking drought on the starch physicochemical properties of waxy maize | |
Huo et al. | Application of Exogenous 6‐benzyladenine at the Silking‐Stage Improves the Starch Quality of Waxy Maize Suffering from Post‐Silking Drought Stress | |
Ke et al. | Differences in starch composition and physiochemical properties are influenced by granule types in wheat and its relatives | |
ZHANG et al. | Identification and gene mapping-based clone of two chalkiness mutants in rice | |
Qin et al. | Effects of the Wx gene on starch biosynthesis, physicochemical wheat flour properties, and dry noodle quality |