RU2015151483A - NEW RECOMBINANT STRAINS (OPTIONS) OF PENICILLIUM VERRUCULOSUM MICELLIUM MUSHROOM AND ENZYME PREPARATIONS INTENDED FOR HYDROLYSIS OF INULO-CONTAINING RAW MATERIALS, AND WAYS OF THEIR RECEIVING - Google Patents

NEW RECOMBINANT STRAINS (OPTIONS) OF PENICILLIUM VERRUCULOSUM MICELLIUM MUSHROOM AND ENZYME PREPARATIONS INTENDED FOR HYDROLYSIS OF INULO-CONTAINING RAW MATERIALS, AND WAYS OF THEIR RECEIVING Download PDF

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RU2015151483A
RU2015151483A RU2015151483A RU2015151483A RU2015151483A RU 2015151483 A RU2015151483 A RU 2015151483A RU 2015151483 A RU2015151483 A RU 2015151483A RU 2015151483 A RU2015151483 A RU 2015151483A RU 2015151483 A RU2015151483 A RU 2015151483A
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penicillium verruculosum
fungus penicillium
verruculosum
endoglucanase
glucosidase
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RU2015151483A
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RU2646136C2 (en
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Аркадий Пантелеймонович Синицын
Александра Михайловна Рожкова
Иван Никитич Зоров
Ольга Аркадьевна Синицына
Павел Валерьевич Волков
Елена Николаевна Ефременко
Вероника Юрьевна Матыс
Виталий Алексеевич Немашкалов
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Федеральное государственное учреждение "Федеральный исследовательский центр "Фундаментальные основы биотехнологии" Российской академии наук" (ФИЦ Биотехнологии РАН)
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    • C12N15/09Recombinant DNA-technology
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    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2434Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2434Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/244Endo-1,3(4)-beta-glucanase (3.2.1.6)
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2434Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2445Beta-glucosidase (3.2.1.21)
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    • C12R2001/00Microorganisms ; Processes using microorganisms
    • C12R2001/645Fungi ; Processes using fungi
    • C12R2001/80Penicillium

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  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Claims (4)

1. Рекомбинантный штамм гриба Penicillium verruculosum PVERINU13 (ВКПМ F-1292) - продуцент комплекса целлюлолитических ферментов, включающих целлобиогидролазы I и II, эндоглюканазу, бета-глюкозидазу гриба Penicillium verruculosum и гетерологичную экзоинулиназу из Aspergillus awamori, и обладающий преимущественной активностью по инулину топинамбура.1. The recombinant strain of the fungus Penicillium verruculosum PVERINU13 (VKPM F-1292) is a producer of a complex of cellulolytic enzymes, including cellobiohydrolases I and II, endoglucanase, beta-glucosidase of the fungus Penicillium verruculosum and heterologous exoinuinus from Isoinillus superior iperinumus aworminusus superior. 2. Рекомбинантный штамм гриба Penicillium verruculosum PVERINU18 (ВКПМ F-1291) - продуцент высокоактивного комплекса целлюлолитических ферментов, включающих целлобиогидролазы I и II, эндоглюканазу, бета-глюкозидазу гриба Penicillium verruculosum и гетерологичную экзоинулиназу из Aspergillus awamori, и обладающий активностью по инулину топинамбура и активностью по микрокристаллической целлюлозе.2. The recombinant strain of the fungus Penicillium verruculosum PVERINU18 (VKPM F-1291) is a producer of a highly active complex of cellulolytic enzymes, including cellobiohydrolases I and II, endoglucanase, beta-glucosidase of the fungus Penicillium verruculosum and heterologous exoinulinu activity of Asoinuinuormu from Isoinuinuormu asuinuinuorm from awinuinuormu on microcrystalline cellulose. 3. Способ получения ферментного препарата целлюлаз, таких как целлобиогидролазы I и II, эндоглюканаза, бета-глюкозидаза гриба Penicillium verruculosum, и экзоинулиназы, обладающего преимущественной активностью по инулину топинамбура, и заключающийся в культивировании штамма гриба Penicillium verruculosum PVERINU13 (ВКПМ F-1292), по п. 1 с последующим концентрированием культуральной жидкости и сушкой.3. A method of obtaining an enzymatic preparation of cellulases, such as cellobiohydrolases I and II, endoglucanase, beta-glucosidase of the fungus Penicillium verruculosum, and exoinulinase, which has a primary effect on Jerusalem artichoke, and which consists in the cultivation of the strain of the fungus Penicillium verruculosum PVERINU13 (VK) BK according to claim 1, followed by concentration of the culture fluid and drying. 4. Способ получения ферментного препарата целлюлаз, таких как целлобиогидролазы I и II, эндоглюканаза, бета-глюкозидаза гриба Penicillium verruculosum, и экзоинулиназы, обладающего активностью по микрокристаллической целлюлозе и инулину топинамбура, и заключающийся в культивировании штамма гриба Penicillium verruculosum PVER18 (ВКПМ F-1291), по п. 2 с последующим концентрированием культуральной жидкости и сушкой.4. A method of obtaining an enzymatic preparation of cellulases, such as cellobiohydrolases I and II, endoglucanase, beta-glucosidase of the fungus Penicillium verruculosum, and exoinulinase, which has activity on microcrystalline cellulose and Jerusalem artichoke inulin, and which consists in the cultivation of the strain of the fungus Penicillium FER91Pru91um Fru91um ), according to claim 2, followed by concentration of the culture fluid and drying.
RU2015151483A 2015-12-02 2015-12-02 Recombinant strain of mycelial fungus penicillium verruculosum (versions) and a method of producing enzyme preparation with its use (variants) RU2646136C2 (en)

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RU2748229C1 (en) * 2020-05-22 2021-05-21 Федеральное государственное учреждение "Федеральный исследовательский центр "Фундаментальные основы биотехнологии" Российской академии наук" (ФИЦ Биотехнологии РАН) Method for producing fumaric acid

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RU2378372C2 (en) * 2008-03-03 2010-01-10 ООО НПК "Фермтек" Genetic maker to ensure expression of target homologous and heterologous genes in cells of filamentous fungus penicillium verruculosum; used as host, method for making strain of fungus penicillium verruculosum and method for making enzymatic agent
RU2532840C2 (en) * 2012-09-19 2014-11-10 Федеральное государственное бюджетное учреждение науки Институт биохимии и физиологии микроорганизмов им. Г.К. Скрябина Российской академии наук (ИБФМ РАН) FUNGAL STRAIN Penicillium verruculosum B10 EGII PRODUCER OF ENDO-1,3/1,4-β-GLUCANASE, CELLULASE, β-GLUCOSIDASE AND XYLANASE AND METHOD OF PRODUCTION OF FODDER COMPLEX ENZYME PREPARATION
RU2550044C2 (en) * 2013-09-10 2015-05-10 Федеральное государственное бюджетное учреждение науки Институт биохимии им. А.Н. Баха Российской академии наук (ИНБИ РАН) GENETIC FUSION-CONSTRUCTION FOR PROVIDING EXPRESSION OF MULTIENZYMATIC COMPLEX OF CARBOHYDRASES IN CELLS OF MYCELIUM FUNGUS Penicillium verruculosum, APPLIED AS HOST, METHOD OF OBTAINING RECOMBINANT STRAIN OF FUNGUS Penicillium verruculosum AND METHOD OF OBTAINING ENZYMATIC MEDICATION ON ITS BASE
CN103981112A (en) * 2014-05-21 2014-08-13 山东大学 Double-promoter multi-copy recombinant pichia pastoris strain for highly producing endo-inulinase

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