FI20125550A - Improved endoglucanases for handling cellulosic material - Google Patents

Improved endoglucanases for handling cellulosic material Download PDF

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Publication number
FI20125550A
FI20125550A FI20125550A FI20125550A FI20125550A FI 20125550 A FI20125550 A FI 20125550A FI 20125550 A FI20125550 A FI 20125550A FI 20125550 A FI20125550 A FI 20125550A FI 20125550 A FI20125550 A FI 20125550A
Authority
FI
Finland
Prior art keywords
cellulosic material
production
fermentable sugars
endoglucanases
improved
Prior art date
Application number
FI20125550A
Other languages
Finnish (fi)
Swedish (sv)
Other versions
FI124476B (en
Inventor
Taija Leinonen
Alexandra Komander
Kim Langfelder
Jari Vehmaanperae
Terhi Puranen
Original Assignee
Roal Oy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to FI20125550A priority Critical patent/FI124476B/en
Application filed by Roal Oy filed Critical Roal Oy
Priority to EP13793173.9A priority patent/EP2855673B1/en
Priority to DK13793173.9T priority patent/DK2855673T3/en
Priority to PCT/FI2013/050564 priority patent/WO2013175074A1/en
Priority to AP2014008135A priority patent/AP2014008135A0/en
Priority to CA2872670A priority patent/CA2872670C/en
Priority to CN201380027157.7A priority patent/CN104995296B/en
Priority to BR112014028716A priority patent/BR112014028716A2/en
Publication of FI20125550A publication Critical patent/FI20125550A/en
Application granted granted Critical
Publication of FI124476B publication Critical patent/FI124476B/en
Priority to ZA2014/08383A priority patent/ZA201408383B/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P2203/00—Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Paper (AREA)
  • Chemical Or Physical Treatment Of Fibers (AREA)

Abstract

The present invention relates to production of fermentable sugars from lignocellulosic material by enzymatic conversion. The fermentable sugars are useful e.g. in the production of bioethanol. Novel polypeptides having endoglu-canase activity, polynucleotides encoding them and vectors and host cells containing the polynucleotides are disclosed. A method for treating cellulosic material with the novel endoglucanase as well as uses of the enzymes and enzyme preparations and a method of preparing them are described.
FI20125550A 2012-05-24 2012-05-24 Enhanced endoglucanases for the treatment of cellulosic material FI124476B (en)

Priority Applications (9)

Application Number Priority Date Filing Date Title
FI20125550A FI124476B (en) 2012-05-24 2012-05-24 Enhanced endoglucanases for the treatment of cellulosic material
DK13793173.9T DK2855673T3 (en) 2012-05-24 2013-05-23 IMPROVED ENDOGLUCANASES FOR TREATMENT OF CELLULOS MATERIAL
PCT/FI2013/050564 WO2013175074A1 (en) 2012-05-24 2013-05-23 Improved endoglucanases for treatment of cellulosic material
AP2014008135A AP2014008135A0 (en) 2012-05-24 2013-05-23 Improved endoglucanases for treatment of cellulosic material
EP13793173.9A EP2855673B1 (en) 2012-05-24 2013-05-23 Improved endoglucanases for treatment of cellulosic material
CA2872670A CA2872670C (en) 2012-05-24 2013-05-23 Improved endoglucanases for treatment of cellulosic material
CN201380027157.7A CN104995296B (en) 2012-05-24 2013-05-23 For handling the improved endoglucanase of cellulosic material
BR112014028716A BR112014028716A2 (en) 2012-05-24 2013-05-23 improved endoglycanases for treatment of cellulosic material
ZA2014/08383A ZA201408383B (en) 2012-05-24 2014-11-13 Improved endoglucanases for treatment of cellulosic material

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FI20125550A FI124476B (en) 2012-05-24 2012-05-24 Enhanced endoglucanases for the treatment of cellulosic material
FI20125550 2012-05-24

Publications (2)

Publication Number Publication Date
FI20125550A true FI20125550A (en) 2013-11-25
FI124476B FI124476B (en) 2014-09-15

Family

ID=49623218

Family Applications (1)

Application Number Title Priority Date Filing Date
FI20125550A FI124476B (en) 2012-05-24 2012-05-24 Enhanced endoglucanases for the treatment of cellulosic material

Country Status (9)

Country Link
EP (1) EP2855673B1 (en)
CN (1) CN104995296B (en)
AP (1) AP2014008135A0 (en)
BR (1) BR112014028716A2 (en)
CA (1) CA2872670C (en)
DK (1) DK2855673T3 (en)
FI (1) FI124476B (en)
WO (1) WO2013175074A1 (en)
ZA (1) ZA201408383B (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106011198A (en) * 2016-06-16 2016-10-12 桐庐唐赢生物科技有限公司 Method for preparing artificial starch, cellulosic ethanol and single-cell protein by taking agroforestry lignocellulose biomass as raw material
EP3653706A1 (en) * 2018-11-13 2020-05-20 AB Enzymes Oy Fusion protein
CN110923182B (en) * 2019-12-06 2022-09-13 四川轻化工大学 High-efficiency acid-resistant endoglucanase engineering bacteria based on vinasse and application thereof
CN114574370B (en) * 2022-03-18 2023-09-15 贵州医科大学 Screening method of degradation biomass strain, trichoderma and application

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK1272643T4 (en) * 2000-03-20 2014-05-26 Basf Se Talaromyces emersonii beta-glucanases
FI120045B (en) * 2005-12-22 2009-06-15 Roal Oy Treatment of cellulose materials and enzymes useful therein
EP2374877A3 (en) * 2006-03-30 2012-11-07 Novozymes A/S Polypeptides having endoglucanase activity and polynucleotides encoding same
WO2007118935A1 (en) * 2006-04-13 2007-10-25 Ab Enzymes Oy Enzyme fusion proteins and their use
CN101809150B (en) * 2007-05-31 2013-09-04 诺维信股份有限公司 Methods of increasing the cellulolytic enhancing activity of a polypeptide
CN106167796B (en) * 2009-11-06 2019-06-04 诺维信股份有限公司 Composition for saccharified cellulosic material
CA3152266A1 (en) * 2010-06-03 2011-12-08 Lallemand Hungary Liquidity Management Llc Yeast expressing saccharolytic enzymes for consolidated bioprocessing using starch and cellulose
BR112013009026A2 (en) * 2010-11-12 2016-07-12 Novozymes Inc isolated polypeptide, recombinant host cell, methods for producing a polypeptide, a precursor cell mutant, a protein, and a fermentation product, for inhibiting expression of a polypeptide, for degrading or converting a cellulosic material, and method for fermenting a cellulosic material, transgenic plant, plant part or plant cell, double stranded inhibitor mRNA molecule, isolated polynucleotide, and integral broth formulation or cell culture composition

Also Published As

Publication number Publication date
CN104995296A (en) 2015-10-21
EP2855673A4 (en) 2016-05-11
BR112014028716A2 (en) 2017-07-25
CN104995296B (en) 2019-01-15
CA2872670C (en) 2021-07-27
FI124476B (en) 2014-09-15
AP2014008135A0 (en) 2014-12-31
CA2872670A1 (en) 2013-11-28
EP2855673B1 (en) 2017-05-10
DK2855673T3 (en) 2017-06-19
ZA201408383B (en) 2016-07-27
EP2855673A1 (en) 2015-04-08
WO2013175074A1 (en) 2013-11-28

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