WO2015042543A3 - Biofuel production enzymes and uses thereof - Google Patents

Biofuel production enzymes and uses thereof Download PDF

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Publication number
WO2015042543A3
WO2015042543A3 PCT/US2014/056827 US2014056827W WO2015042543A3 WO 2015042543 A3 WO2015042543 A3 WO 2015042543A3 US 2014056827 W US2014056827 W US 2014056827W WO 2015042543 A3 WO2015042543 A3 WO 2015042543A3
Authority
WO
WIPO (PCT)
Prior art keywords
biofuels
ancestral
microorganisms
useful
production
Prior art date
Application number
PCT/US2014/056827
Other languages
French (fr)
Other versions
WO2015042543A2 (en
Inventor
Julio M. Fernandez
Raul Perez-Jimenez
Original Assignee
The Trustees Of Columbia University In The City Of New York
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The Trustees Of Columbia University In The City Of New York filed Critical The Trustees Of Columbia University In The City Of New York
Publication of WO2015042543A2 publication Critical patent/WO2015042543A2/en
Publication of WO2015042543A3 publication Critical patent/WO2015042543A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P2203/00—Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

These enzymes are useful for the production of Cellulosic Ethanol for biofuels. Some aspects of the present invention provide for ancestral fungal cellulases. Cellulase enzymes are useful for the production of cellulosic ethanol for biofuels. In some embodiments, ancestral cellulases can be used for the hydrolysis of carbohydrate polymers that comprise cellulose. Some aspects of the present invention provide for microorganisms that express an ancestral cellulase. Microorganisms are useful for the production of cellulosic ethanol for biofuels. In some embodiments, microorganisms can be used for the hydrolysis and/or fermentation of cellulose. In another aspect, the present invention provides for an isolated polypeptide comprising about 90% identity to amino acid sequences.
PCT/US2014/056827 2013-09-20 2014-09-22 Biofuel production enzymes and uses thereof WO2015042543A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361880466P 2013-09-20 2013-09-20
US61/880,466 2013-09-20

Publications (2)

Publication Number Publication Date
WO2015042543A2 WO2015042543A2 (en) 2015-03-26
WO2015042543A3 true WO2015042543A3 (en) 2015-05-14

Family

ID=52689611

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2014/056827 WO2015042543A2 (en) 2013-09-20 2014-09-22 Biofuel production enzymes and uses thereof

Country Status (1)

Country Link
WO (1) WO2015042543A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017151957A1 (en) 2016-03-02 2017-09-08 Novozymes A/S Cellobiohydrolase variants and polynucleotides encoding same
CN109182358B (en) * 2018-09-17 2022-02-08 黑龙江省农业科学院耕作栽培研究所 Bacillus cellulase gene FIB1X suitable for returning rice straws to field

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120040408A1 (en) * 2008-06-20 2012-02-16 Decker Stephen R Processing cellulosic biomass
US8163976B2 (en) * 2007-05-23 2012-04-24 The Penn State Research Foundation Compositions and methods relating to transgenic plants and cellulosic ethanol production
US20120129229A1 (en) * 2008-11-21 2012-05-24 Mcbride John Yeast Expressing Cellulases for Simultaneous Saccharification and Fermentation Using Cellulose
US8298795B2 (en) * 2010-08-20 2012-10-30 Codexis, Inc. Use of glycoside hydrolase 61 family proteins in processing of cellulose
US20130217076A1 (en) * 2012-02-17 2013-08-22 Noriko SHISA Polypeptide capable of enhancing cellulosic biomass degradation
WO2013166312A1 (en) * 2012-05-02 2013-11-07 The Trustees Of Columbia University In The City Of New York Biofuel production enzymes and uses thereof

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8163976B2 (en) * 2007-05-23 2012-04-24 The Penn State Research Foundation Compositions and methods relating to transgenic plants and cellulosic ethanol production
US20120040408A1 (en) * 2008-06-20 2012-02-16 Decker Stephen R Processing cellulosic biomass
US20120129229A1 (en) * 2008-11-21 2012-05-24 Mcbride John Yeast Expressing Cellulases for Simultaneous Saccharification and Fermentation Using Cellulose
US8298795B2 (en) * 2010-08-20 2012-10-30 Codexis, Inc. Use of glycoside hydrolase 61 family proteins in processing of cellulose
US20130217076A1 (en) * 2012-02-17 2013-08-22 Noriko SHISA Polypeptide capable of enhancing cellulosic biomass degradation
WO2013166312A1 (en) * 2012-05-02 2013-11-07 The Trustees Of Columbia University In The City Of New York Biofuel production enzymes and uses thereof

Also Published As

Publication number Publication date
WO2015042543A2 (en) 2015-03-26

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