RU2015148624A - Генетическая конструкция рБТШ70 для экспрессии основного человеческого стресс белка, используемого в терапевтических целях, в молоке и линия трансгенных мышей, содержащих данную конструкцию - Google Patents

Генетическая конструкция рБТШ70 для экспрессии основного человеческого стресс белка, используемого в терапевтических целях, в молоке и линия трансгенных мышей, содержащих данную конструкцию Download PDF

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RU2015148624A
RU2015148624A RU2015148624A RU2015148624A RU2015148624A RU 2015148624 A RU2015148624 A RU 2015148624A RU 2015148624 A RU2015148624 A RU 2015148624A RU 2015148624 A RU2015148624 A RU 2015148624A RU 2015148624 A RU2015148624 A RU 2015148624A
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construct
expression
milk
line
transgenic mice
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RU2015148624A
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RU2644663C2 (ru
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Михаил Борисович Евгеньев
Давид Григорьевич Гарбуз
София Георгиевна Георгиева
Вадим Львович Карпов
Алексей Николаевич Краснов
Наталия Валерьевна Сошникова
Алексей Васильевич Дейкин
Ярослав Георгиевич Гурский
Original Assignee
Федеральное Государственное Бюджетное Учреждение Науки Институт Молекулярной Биологии Им. В.А. Энгельгардта Российской Академии Наук (Имб Ран)
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/20—Animal model comprising regulated expression system
    • A01K2217/206—Animal model comprising tissue-specific expression system, e.g. tissue specific expression of transgene, of Cre recombinase
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/01—Animal expressing industrially exogenous proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • C12N2015/8518—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic expressing industrially exogenous proteins, e.g. for pharmaceutical use, human insulin, blood factors, immunoglobulins, pseudoparticles

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Claims (3)

  1. Создание новой экспрессионной системы для экспрессии рекомбинантного человеческого стресс-белка (БТШ70) в молоке.
  2. 1. Генетическая конструкция рБТШ70, содержащая ген человеческого БТШ70 для экспрессии в молоке.
  3. 2. Линия трансгенных мышей, содержащая конструкцию по п. 1, для экспрессии рекомбинантного БТШ70 в молоке.
RU2015148624A 2015-11-12 2015-11-12 Генетическая конструкция рБТШ70 для экспрессии основного человеческого стресс белка в молоке трансгенных животных RU2644663C2 (ru)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU2015148624A RU2644663C2 (ru) 2015-11-12 2015-11-12 Генетическая конструкция рБТШ70 для экспрессии основного человеческого стресс белка в молоке трансгенных животных

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU2015148624A RU2644663C2 (ru) 2015-11-12 2015-11-12 Генетическая конструкция рБТШ70 для экспрессии основного человеческого стресс белка в молоке трансгенных животных

Publications (2)

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RU2015148624A true RU2015148624A (ru) 2017-05-23
RU2644663C2 RU2644663C2 (ru) 2018-02-13

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RU2015148624A RU2644663C2 (ru) 2015-11-12 2015-11-12 Генетическая конструкция рБТШ70 для экспрессии основного человеческого стресс белка в молоке трансгенных животных

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Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69027683T2 (de) * 1989-12-01 1997-02-13 Pharming Bv Herstellung rekombinanter polypeptide durch rinder und transgene methoden
US7115796B2 (en) * 2002-05-06 2006-10-03 Animal Technology Institute Transgenic pig containing heat shock protein 70 transgene

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Effective date: 20191113