RU2015121789A - METHOD FOR COLLECTING THE MAXIMUM NUMBER OF MATURE (= FERTILIZED) EGGS (IN VITRO) FROM TRICHURIS (= TRICHOCEPHALUS) SUIS - Google Patents

METHOD FOR COLLECTING THE MAXIMUM NUMBER OF MATURE (= FERTILIZED) EGGS (IN VITRO) FROM TRICHURIS (= TRICHOCEPHALUS) SUIS Download PDF

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RU2015121789A
RU2015121789A RU2015121789A RU2015121789A RU2015121789A RU 2015121789 A RU2015121789 A RU 2015121789A RU 2015121789 A RU2015121789 A RU 2015121789A RU 2015121789 A RU2015121789 A RU 2015121789A RU 2015121789 A RU2015121789 A RU 2015121789A
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mature
trichuris
suis
trichocephalus
females
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RU2015121789A
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Russian (ru)
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RU2609145C2 (en
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Валерий Евгеньевич Пасечник
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ФАНО России Федеральное государственное бюджетное научное учреждение Всероссийский научно-исследовательский институт фундаментальной и прикладной паразитологии животных и растений им. К.И. Скрябина (ФГБНУ "ВНИИП им. К.И. Скрябина")
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
    • A61D99/00—Subject matter not provided for in other groups of this subclass

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  • Investigating Or Analysing Biological Materials (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

Способ сбора максимального количества зрелых (=оплодотворенных) яиц (in vitro) от самок возбудителя паразитарного зооноза Trichuris (=Trichocephalus) suis, заключающийся в отборе индивидуально и только живых, активных, половозрелых самок Trichuris suis из толстой, слепой кишок спонтанно зараженных возбудителем трихоцефалеза, при исследовании гельминтологическими методами на вскрытии диких кабанов или/и домашних свиней, в отдельные пробирки с раствором Rhode-Saito, который по своему химическому составу приближается к составу пищеварительного тракта млекопитающих, и экспозицией пробирок с живыми, активными, половозрелыми самками Trichuris suis в условиях приближенных к жизнедеятельности паразита в организме свиней: при t=38°C-40°C в течение 5 часов в термостате.A method of collecting the maximum number of mature (= fertilized) eggs (in vitro) from females of the pathogen parasitic zoonosis Trichuris (= Trichocephalus) suis, which consists in the selection of individually and only live, active, mature females Trichuris suis from the large, blind gut spontaneously infected with the pathogen trichocephalus when using helminthological methods to open wild boars and / or domestic pigs, in separate tubes with a Rhode-Saito solution, which in its chemical composition approaches the composition of the digestive tract of mammals, and exposure of test tubes with live, active, mature females of Trichuris suis under conditions close to the vital activity of the parasite in the pigs: at t = 38 ° C-40 ° C for 5 hours in a thermostat.

Claims (1)

Способ сбора максимального количества зрелых (=оплодотворенных) яиц (in vitro) от самок возбудителя паразитарного зооноза Trichuris (=Trichocephalus) suis, заключающийся в отборе индивидуально и только живых, активных, половозрелых самок Trichuris suis из толстой, слепой кишок спонтанно зараженных возбудителем трихоцефалеза, при исследовании гельминтологическими методами на вскрытии диких кабанов или/и домашних свиней, в отдельные пробирки с раствором Rhode-Saito, который по своему химическому составу приближается к составу пищеварительного тракта млекопитающих, и экспозицией пробирок с живыми, активными, половозрелыми самками Trichuris suis в условиях приближенных к жизнедеятельности паразита в организме свиней: при t=38°C-40°C в течение 5 часов в термостате. A method of collecting the maximum number of mature (= fertilized) eggs (in vitro) from females of the pathogen parasitic zoonosis Trichuris (= Trichocephalus) suis, which consists in the selection of individually and only live, active, mature females Trichuris suis from the large, blind gut spontaneously infected with the pathogen trichocephalus when using helminthological methods to open wild boars and / or domestic pigs, in separate tubes with a Rhode-Saito solution, which in its chemical composition approaches the composition of the digestive tract of mammals, and exposure of test tubes with live, active, mature females of Trichuris suis under conditions close to the vital activity of the parasite in the pigs: at t = 38 ° C-40 ° C for 5 hours in a thermostat.
RU2015121789A 2015-06-08 2015-06-08 Method of collecting maximum amount of mature (=fertilized) eggs (in vitro) of females of parasitic zoonosis infectious agent trichuris (=trichocephalus) suis RU2609145C2 (en)

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RU2015121789A RU2609145C2 (en) 2015-06-08 2015-06-08 Method of collecting maximum amount of mature (=fertilized) eggs (in vitro) of females of parasitic zoonosis infectious agent trichuris (=trichocephalus) suis

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RU2015121789A RU2609145C2 (en) 2015-06-08 2015-06-08 Method of collecting maximum amount of mature (=fertilized) eggs (in vitro) of females of parasitic zoonosis infectious agent trichuris (=trichocephalus) suis

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RU2712892C1 (en) * 2019-05-28 2020-01-31 Федеральное государственное бюджетное учреждение "Федеральный научный центр-Всероссийский научно-исследовательский институт экспериментальной ветеринарии им. К.И. Скрябина и Я.Р. Коваленко" (ФГБНУ ФНЦ ВИЭВ РАН) Method of in-vitro egg collection from trichuris (= trichocephalus) ovis, which causes trichuriasis of domestic and wild ruminant

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RU2193310C1 (en) * 2001-03-30 2002-11-27 Научно-исследовательский ветеринарный институт Нечерноземной зоны Российской Федерации Method for cultivation of trematode eggs
EP2123774A1 (en) * 2008-05-21 2009-11-25 Dr. Falk Pharma Gmbh Method for characterising the biological activity of Helminth eggs

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