CL2017002864A1 - Methods of administration of agents to eggs and embryos of oviparous aquatic animals for drug screening, agent toxicity testing and production of infertile fish. - Google Patents

Methods of administration of agents to eggs and embryos of oviparous aquatic animals for drug screening, agent toxicity testing and production of infertile fish.

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Publication number
CL2017002864A1
CL2017002864A1 CL2017002864A CL2017002864A CL2017002864A1 CL 2017002864 A1 CL2017002864 A1 CL 2017002864A1 CL 2017002864 A CL2017002864 A CL 2017002864A CL 2017002864 A CL2017002864 A CL 2017002864A CL 2017002864 A1 CL2017002864 A1 CL 2017002864A1
Authority
CL
Chile
Prior art keywords
eggs
methods
oviparous
administration
agents
Prior art date
Application number
CL2017002864A
Other languages
Spanish (es)
Inventor
Yonathan Zohar
Ten-Tsao Wong
Original Assignee
Univ Maryland
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Maryland filed Critical Univ Maryland
Publication of CL2017002864A1 publication Critical patent/CL2017002864A1/en

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2207/00—Modified animals
    • A01K2207/05—Animals modified by non-integrating nucleic acids, e.g. antisense, RNAi, morpholino, episomal vector, for non-therapeutic purpose
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2207/00—Modified animals
    • A01K2207/20—Animals treated with compounds which are neither proteins nor nucleic acids
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/40—Fish
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/02—Animal zootechnically ameliorated
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/32—Chemical structure of the sugar
    • C12N2310/323—Chemical structure of the sugar modified ring structure
    • C12N2310/3233—Morpholino-type ring
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/30—Special therapeutic applications
    • C12N2320/32—Special delivery means, e.g. tissue-specific
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81—Aquaculture, e.g. of fish
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A90/00—Technologies having an indirect contribution to adaptation to climate change
    • Y02A90/40—Monitoring or fighting invasive species

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Environmental Sciences (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Animal Husbandry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Veterinary Medicine (AREA)
  • Farming Of Fish And Shellfish (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

<p>Se proporcionan métodos para la administración de al menos un agente a uno o más huevos de un animal acuático ovíparo que incluye poner en contacto el o los huevos fertilizados o no fertilizados de dicho animal acuático ovíparo con al menos un agente en presencia de un compuesto que contiene guanidina capaz de aumentar la permeabilidad del corion del (de los) huevo(s). Se proporcionan métodos para el tamizaje de fármacos y ensayos de toxicidad de compuestos. Se proporcionan además métodos para la producción de peces y animales acuáticos estériles reproductivamente para la acuicultura, el comercio de acuarios, y el control de especies invasoras. Los métodos incluyen la disrupción del desarrollo gonadal mediante la administración de agentes que conducen al fallo del desarrollo de gónadas fértiles. Los agentes pueden administrarse directamente a los huevos antes de la fertilización o después de la fertilización al poner en contacto los huevos en un medio de inmersión que incluye el agente de interés.</p><p> Methods are provided for the administration of at least one agent to one or more eggs of an oviparous aquatic animal which includes contacting the fertilized or unfertilized eggs of said oviparous aquatic animal with at least one agent in the presence of a compound containing guanidine capable of increasing the permeability of the chorion of the egg (s). Methods for drug screening and compound toxicity tests are provided. Methods are also provided for the production of reproductively sterile fish and aquatic animals for aquaculture, aquarium trade, and control of invasive species. The methods include disruption of gonadal development through the administration of agents that lead to the failure of the development of fertile gonads. Agents can be administered directly to the eggs before fertilization or after fertilization by contacting the eggs in an immersion medium that includes the agent of interest. </p>

CL2017002864A 2015-05-19 2017-11-10 Methods of administration of agents to eggs and embryos of oviparous aquatic animals for drug screening, agent toxicity testing and production of infertile fish. CL2017002864A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562163511P 2015-05-19 2015-05-19
US201662274958P 2016-01-05 2016-01-05

Publications (1)

Publication Number Publication Date
CL2017002864A1 true CL2017002864A1 (en) 2018-05-18

Family

ID=56084421

Family Applications (1)

Application Number Title Priority Date Filing Date
CL2017002864A CL2017002864A1 (en) 2015-05-19 2017-11-10 Methods of administration of agents to eggs and embryos of oviparous aquatic animals for drug screening, agent toxicity testing and production of infertile fish.

Country Status (13)

Country Link
US (1) US10709119B2 (en)
EP (1) EP3297429A2 (en)
JP (1) JP6756960B2 (en)
CN (1) CN108040459B (en)
AU (1) AU2016265929B2 (en)
BR (1) BR112017024662A2 (en)
CA (1) CA2984644A1 (en)
CL (1) CL2017002864A1 (en)
DK (1) DK180094B1 (en)
IL (1) IL255338B (en)
MX (1) MX2017014856A (en)
RU (1) RU2741645C2 (en)
WO (1) WO2016187198A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX369329B (en) 2013-11-15 2019-11-05 Univ Maryland Method of producing infertile fish and egg-producing aquatic animals and of delivering compounds into eggs and embryos.
EP3723479B1 (en) 2017-12-15 2024-05-22 ACD Pharmaceuticals AS Methods for the production of sterile fish
WO2020125848A1 (en) * 2018-12-17 2020-06-25 Alfred-Wegener-Institut, Helmholtz-Zentrum für Polar- und Meeresforschung Method for producing caviar or a caviar-like product from living, mature eggs of fish or crustaceans, and products of this kind

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7115794B2 (en) * 2003-05-19 2006-10-03 University Of Hawaii Method for direct nucleic acid uptake
US7194978B2 (en) 2003-08-22 2007-03-27 University Of Maryland Biotechnology Institute Inducing sterility in fish by disrupting the development of the GnRH system
CN1294808C (en) 2003-09-26 2007-01-17 中国科学院水生生物研究所 Method for preparing sterile transgenic fish
CN1274822C (en) 2004-02-19 2006-09-13 武汉康祥科技发展有限公司 Method for chemically inducing gynogenesis of living fishes
CN101553210A (en) * 2006-09-20 2009-10-07 奈克斯特安全有限公司 Methods and systems of delivering medication via inhalation
CN101652475B (en) 2007-01-26 2014-03-19 辛那杰瓦生物制药股份有限公司 Transgene expression in avians
US7935816B2 (en) 2007-10-25 2011-05-03 Gene Tools, Llc Molecular transporter compositions comprising dendrimeric oligoguanidine with a triazine core that facilitate delivery into cells in vivo
US8383880B2 (en) 2009-02-18 2013-02-26 Academia Sinica Infertility control of genetically modified fish
WO2011018798A2 (en) * 2009-08-14 2011-02-17 Indian Association For The Cultivation Of Science Morpholino-based antisense agent
US8921643B2 (en) * 2010-02-09 2014-12-30 National University Corporation Hokkaido University Method for acquiring genetically identical gamete from lethal fish haploid-derived germ cell via germ line chimera
CA2825994A1 (en) 2011-02-01 2012-08-09 Ten-Tsao WONG Efficient sterilization of fish by disruption of germ cell development
GB201117538D0 (en) 2011-10-11 2011-11-23 Royal Veterinary College The Methods
RU2504389C2 (en) * 2012-02-16 2014-01-20 Федеральное государственное бюджетное учреждение науки Институт биохимии и физиологии микроорганизмов им. Г.К. Скрябина Российской академии наук (ИБФМ РАН) METHOD FOR PORE FORMATION IN HEPATOCYTE MEMBRANES BY Bacillus cereus HEMOLYSIN II PROCESSING
US9538734B2 (en) 2013-03-12 2017-01-10 The General Hospital Corporation Germ cell ablation compounds and uses thereof
MX369329B (en) * 2013-11-15 2019-11-05 Univ Maryland Method of producing infertile fish and egg-producing aquatic animals and of delivering compounds into eggs and embryos.

Also Published As

Publication number Publication date
JP2018515116A (en) 2018-06-14
US20180295817A1 (en) 2018-10-18
CA2984644A1 (en) 2016-11-24
IL255338B (en) 2022-05-01
RU2017142635A3 (en) 2019-12-16
CN108040459B (en) 2021-09-21
JP6756960B2 (en) 2020-09-16
AU2016265929A1 (en) 2017-11-09
RU2017142635A (en) 2019-06-20
WO2016187198A3 (en) 2017-01-12
AU2016265929B2 (en) 2022-03-31
MX2017014856A (en) 2018-04-20
IL255338A0 (en) 2017-12-31
EP3297429A2 (en) 2018-03-28
WO2016187198A2 (en) 2016-11-24
CN108040459A (en) 2018-05-15
DK180094B1 (en) 2020-04-27
RU2741645C2 (en) 2021-01-28
US10709119B2 (en) 2020-07-14
DK201770877A1 (en) 2017-11-27
BR112017024662A2 (en) 2018-09-11

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