RU2015116883A - Применение ионообменной мембраны для удаления примесей из конъюгатов клеточносвязывающего агента с цитотоксическим агентом - Google Patents
Применение ионообменной мембраны для удаления примесей из конъюгатов клеточносвязывающего агента с цитотоксическим агентом Download PDFInfo
- Publication number
- RU2015116883A RU2015116883A RU2015116883A RU2015116883A RU2015116883A RU 2015116883 A RU2015116883 A RU 2015116883A RU 2015116883 A RU2015116883 A RU 2015116883A RU 2015116883 A RU2015116883 A RU 2015116883A RU 2015116883 A RU2015116883 A RU 2015116883A
- Authority
- RU
- Russia
- Prior art keywords
- mixture
- cell
- agent
- cytotoxic agent
- binding agent
- Prior art date
Links
- 239000011230 binding agent Substances 0.000 title claims abstract 70
- 229940127089 cytotoxic agent Drugs 0.000 title claims abstract 70
- 239000002254 cytotoxic agent Substances 0.000 title claims abstract 70
- 231100000599 cytotoxic agent Toxicity 0.000 title claims abstract 70
- 239000012535 impurity Substances 0.000 title claims abstract 33
- 239000003014 ion exchange membrane Substances 0.000 title 1
- 238000000034 method Methods 0.000 claims abstract 101
- 239000000203 mixture Substances 0.000 claims abstract 83
- 238000005377 adsorption chromatography Methods 0.000 claims abstract 25
- 230000001588 bifunctional effect Effects 0.000 claims abstract 19
- 239000003431 cross linking reagent Substances 0.000 claims abstract 18
- 239000012528 membrane Substances 0.000 claims abstract 17
- 238000009295 crossflow filtration Methods 0.000 claims abstract 12
- 238000005342 ion exchange Methods 0.000 claims abstract 12
- 238000001914 filtration Methods 0.000 claims abstract 6
- 238000001556 precipitation Methods 0.000 claims abstract 6
- 238000001179 sorption measurement Methods 0.000 claims abstract 6
- 238000004587 chromatography analysis Methods 0.000 claims 13
- 238000004255 ion exchange chromatography Methods 0.000 claims 13
- 239000000243 solution Substances 0.000 claims 12
- OWXMKDGYPWMGEB-UHFFFAOYSA-N HEPPS Chemical compound OCCN1CCN(CCCS(O)(=O)=O)CC1 OWXMKDGYPWMGEB-UHFFFAOYSA-N 0.000 claims 9
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 claims 6
- LVQFQZZGTZFUNF-UHFFFAOYSA-N 2-hydroxy-3-[4-(2-hydroxy-3-sulfonatopropyl)piperazine-1,4-diium-1-yl]propane-1-sulfonate Chemical compound OS(=O)(=O)CC(O)CN1CCN(CC(O)CS(O)(=O)=O)CC1 LVQFQZZGTZFUNF-UHFFFAOYSA-N 0.000 claims 6
- JOCBASBOOFNAJA-UHFFFAOYSA-N N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid Chemical compound OCC(CO)(CO)NCCS(O)(=O)=O JOCBASBOOFNAJA-UHFFFAOYSA-N 0.000 claims 6
- 238000001042 affinity chromatography Methods 0.000 claims 6
- 239000006172 buffering agent Substances 0.000 claims 6
- 239000000539 dimer Substances 0.000 claims 6
- 238000004191 hydrophobic interaction chromatography Methods 0.000 claims 6
- 229910052588 hydroxylapatite Inorganic materials 0.000 claims 6
- XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 claims 6
- -1 ester ε-maleimidocaproic acid hydroxysuccinimide ester Chemical class 0.000 claims 4
- 238000010791 quenching Methods 0.000 claims 4
- 239000007995 HEPES buffer Substances 0.000 claims 3
- 239000007996 HEPPS buffer Substances 0.000 claims 3
- GIZQLVPDAOBAFN-UHFFFAOYSA-N HEPPSO Chemical compound OCCN1CCN(CC(O)CS(O)(=O)=O)CC1 GIZQLVPDAOBAFN-UHFFFAOYSA-N 0.000 claims 3
- 239000008351 acetate buffer Substances 0.000 claims 3
- 239000000919 ceramic Substances 0.000 claims 3
- 239000012634 fragment Substances 0.000 claims 3
- 230000002209 hydrophobic effect Effects 0.000 claims 3
- 230000006698 induction Effects 0.000 claims 3
- 239000002184 metal Substances 0.000 claims 3
- 239000008363 phosphate buffer Substances 0.000 claims 3
- 238000004366 reverse phase liquid chromatography Methods 0.000 claims 3
- YCLWMUYXEGEIGD-UHFFFAOYSA-M sodium;2-hydroxy-3-[4-(2-hydroxyethyl)piperazin-1-yl]propane-1-sulfonate Chemical compound [Na+].OCCN1CCN(CC(O)CS([O-])(=O)=O)CC1 YCLWMUYXEGEIGD-UHFFFAOYSA-M 0.000 claims 3
- 239000008362 succinate buffer Substances 0.000 claims 3
- LLXVXPPXELIDGQ-UHFFFAOYSA-N (2,5-dioxopyrrolidin-1-yl) 3-(2,5-dioxopyrrol-1-yl)benzoate Chemical compound C=1C=CC(N2C(C=CC2=O)=O)=CC=1C(=O)ON1C(=O)CCC1=O LLXVXPPXELIDGQ-UHFFFAOYSA-N 0.000 claims 2
- JWDFQMWEFLOOED-UHFFFAOYSA-N (2,5-dioxopyrrolidin-1-yl) 3-(pyridin-2-yldisulfanyl)propanoate Chemical compound O=C1CCC(=O)N1OC(=O)CCSSC1=CC=CC=N1 JWDFQMWEFLOOED-UHFFFAOYSA-N 0.000 claims 2
- JSHOVKSMJRQOGY-UHFFFAOYSA-N (2,5-dioxopyrrolidin-1-yl) 4-(pyridin-2-yldisulfanyl)butanoate Chemical compound O=C1CCC(=O)N1OC(=O)CCCSSC1=CC=CC=N1 JSHOVKSMJRQOGY-UHFFFAOYSA-N 0.000 claims 2
- GTBCXYYVWHFQRS-UHFFFAOYSA-N (2,5-dioxopyrrolidin-1-yl) 4-(pyridin-2-yldisulfanyl)pentanoate Chemical compound C=1C=CC=NC=1SSC(C)CCC(=O)ON1C(=O)CCC1=O GTBCXYYVWHFQRS-UHFFFAOYSA-N 0.000 claims 2
- WCMOHMXWOOBVMZ-UHFFFAOYSA-N (2,5-dioxopyrrolidin-1-yl) 6-[3-(2,5-dioxopyrrol-1-yl)propanoylamino]hexanoate Chemical compound O=C1CCC(=O)N1OC(=O)CCCCCNC(=O)CCN1C(=O)C=CC1=O WCMOHMXWOOBVMZ-UHFFFAOYSA-N 0.000 claims 2
- NCPQROHLJFARLL-UHFFFAOYSA-N 4-(2,5-dioxopyrrol-1-yl)butanoic acid Chemical compound OC(=O)CCCN1C(=O)C=CC1=O NCPQROHLJFARLL-UHFFFAOYSA-N 0.000 claims 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims 2
- 102000000588 Interleukin-2 Human genes 0.000 claims 2
- 108010002350 Interleukin-2 Proteins 0.000 claims 2
- 102000000646 Interleukin-3 Human genes 0.000 claims 2
- 108010002386 Interleukin-3 Proteins 0.000 claims 2
- 102000004388 Interleukin-4 Human genes 0.000 claims 2
- 108090000978 Interleukin-4 Proteins 0.000 claims 2
- 102000004889 Interleukin-6 Human genes 0.000 claims 2
- 108090001005 Interleukin-6 Proteins 0.000 claims 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims 2
- 229930006000 Sucrose Natural products 0.000 claims 2
- 230000032683 aging Effects 0.000 claims 2
- 239000003011 anion exchange membrane Substances 0.000 claims 2
- 238000005341 cation exchange Methods 0.000 claims 2
- 238000006243 chemical reaction Methods 0.000 claims 2
- 239000003795 chemical substances by application Substances 0.000 claims 2
- 239000007979 citrate buffer Substances 0.000 claims 2
- 230000001268 conjugating effect Effects 0.000 claims 2
- 150000002148 esters Chemical class 0.000 claims 2
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 claims 2
- 229940076264 interleukin-3 Drugs 0.000 claims 2
- 229940028885 interleukin-4 Drugs 0.000 claims 2
- 229940100601 interleukin-6 Drugs 0.000 claims 2
- 230000000171 quenching effect Effects 0.000 claims 2
- 239000005720 sucrose Substances 0.000 claims 2
- 229960000575 trastuzumab Drugs 0.000 claims 2
- TYKASZBHFXBROF-UHFFFAOYSA-N (2,5-dioxopyrrolidin-1-yl) 2-(2,5-dioxopyrrol-1-yl)acetate Chemical compound O=C1CCC(=O)N1OC(=O)CN1C(=O)C=CC1=O TYKASZBHFXBROF-UHFFFAOYSA-N 0.000 claims 1
- PVGATNRYUYNBHO-UHFFFAOYSA-N (2,5-dioxopyrrolidin-1-yl) 4-(2,5-dioxopyrrol-1-yl)butanoate Chemical compound O=C1CCC(=O)N1OC(=O)CCCN1C(=O)C=CC1=O PVGATNRYUYNBHO-UHFFFAOYSA-N 0.000 claims 1
- IHVODYOQUSEYJJ-UHFFFAOYSA-N (2,5-dioxopyrrolidin-1-yl) 6-[[4-[(2,5-dioxopyrrol-1-yl)methyl]cyclohexanecarbonyl]amino]hexanoate Chemical compound O=C1CCC(=O)N1OC(=O)CCCCCNC(=O)C(CC1)CCC1CN1C(=O)C=CC1=O IHVODYOQUSEYJJ-UHFFFAOYSA-N 0.000 claims 1
- FUHCFUVCWLZEDQ-UHFFFAOYSA-N 1-(2,5-dioxopyrrolidin-1-yl)oxy-1-oxo-4-(pyridin-2-yldisulfanyl)butane-2-sulfonic acid Chemical compound O=C1CCC(=O)N1OC(=O)C(S(=O)(=O)O)CCSSC1=CC=CC=N1 FUHCFUVCWLZEDQ-UHFFFAOYSA-N 0.000 claims 1
- OJQSISYVGFJJBY-UHFFFAOYSA-N 1-(4-isocyanatophenyl)pyrrole-2,5-dione Chemical compound C1=CC(N=C=O)=CC=C1N1C(=O)C=CC1=O OJQSISYVGFJJBY-UHFFFAOYSA-N 0.000 claims 1
- QPPUSXURIGJCFQ-UHFFFAOYSA-N 2-[(2-iodoacetyl)amino]propanoic acid Chemical compound OC(=O)C(C)NC(=O)CI QPPUSXURIGJCFQ-UHFFFAOYSA-N 0.000 claims 1
- IUTPJBLLJJNPAJ-UHFFFAOYSA-N 3-(2,5-dioxopyrrol-1-yl)propanoic acid Chemical compound OC(=O)CCN1C(=O)C=CC1=O IUTPJBLLJJNPAJ-UHFFFAOYSA-N 0.000 claims 1
- GWEVSJHKQHAKNW-UHFFFAOYSA-N 4-[[1-(2,5-dioxopyrrolidin-1-yl)-2h-pyridin-2-yl]disulfanyl]-2-sulfobutanoic acid Chemical compound OC(=O)C(S(O)(=O)=O)CCSSC1C=CC=CN1N1C(=O)CCC1=O GWEVSJHKQHAKNW-UHFFFAOYSA-N 0.000 claims 1
- FERIUCNNQQJTOY-UHFFFAOYSA-M Butyrate Chemical compound CCCC([O-])=O FERIUCNNQQJTOY-UHFFFAOYSA-M 0.000 claims 1
- FERIUCNNQQJTOY-UHFFFAOYSA-N Butyric acid Natural products CCCC(O)=O FERIUCNNQQJTOY-UHFFFAOYSA-N 0.000 claims 1
- 239000004971 Cross linker Substances 0.000 claims 1
- BWGNESOTFCXPMA-UHFFFAOYSA-N Dihydrogen disulfide Chemical compound SS BWGNESOTFCXPMA-UHFFFAOYSA-N 0.000 claims 1
- 101150029707 ERBB2 gene Proteins 0.000 claims 1
- 102000009024 Epidermal Growth Factor Human genes 0.000 claims 1
- 101150021185 FGF gene Proteins 0.000 claims 1
- 108010017080 Granulocyte Colony-Stimulating Factor Proteins 0.000 claims 1
- 102000004269 Granulocyte Colony-Stimulating Factor Human genes 0.000 claims 1
- 108010017213 Granulocyte-Macrophage Colony-Stimulating Factor Proteins 0.000 claims 1
- 102100039620 Granulocyte-macrophage colony-stimulating factor Human genes 0.000 claims 1
- 101001099381 Homo sapiens Peroxisomal biogenesis factor 19 Proteins 0.000 claims 1
- 102000004877 Insulin Human genes 0.000 claims 1
- 108090001061 Insulin Proteins 0.000 claims 1
- 102000014150 Interferons Human genes 0.000 claims 1
- 108010050904 Interferons Proteins 0.000 claims 1
- 102100028123 Macrophage colony-stimulating factor 1 Human genes 0.000 claims 1
- 101710127797 Macrophage colony-stimulating factor 1 Proteins 0.000 claims 1
- 229930126263 Maytansine Natural products 0.000 claims 1
- 101100369076 Mus musculus Tdgf1 gene Proteins 0.000 claims 1
- GHAZCVNUKKZTLG-UHFFFAOYSA-N N-ethyl-succinimide Natural products CCN1C(=O)CCC1=O GHAZCVNUKKZTLG-UHFFFAOYSA-N 0.000 claims 1
- HDFGOPSGAURCEO-UHFFFAOYSA-N N-ethylmaleimide Chemical compound CCN1C(=O)C=CC1=O HDFGOPSGAURCEO-UHFFFAOYSA-N 0.000 claims 1
- 229910002651 NO3 Inorganic materials 0.000 claims 1
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 claims 1
- 102100038883 Peroxisomal biogenesis factor 19 Human genes 0.000 claims 1
- 101710098940 Pro-epidermal growth factor Proteins 0.000 claims 1
- 229940123237 Taxane Drugs 0.000 claims 1
- 102000004338 Transferrin Human genes 0.000 claims 1
- 108090000901 Transferrin Proteins 0.000 claims 1
- 102000006747 Transforming Growth Factor alpha Human genes 0.000 claims 1
- 101800004564 Transforming growth factor alpha Proteins 0.000 claims 1
- 108010019530 Vascular Endothelial Growth Factors Proteins 0.000 claims 1
- 102000005789 Vascular Endothelial Growth Factors Human genes 0.000 claims 1
- 239000002253 acid Substances 0.000 claims 1
- 230000003432 anti-folate effect Effects 0.000 claims 1
- 229940127074 antifolate Drugs 0.000 claims 1
- 229950002903 bivatuzumab Drugs 0.000 claims 1
- 239000000872 buffer Substances 0.000 claims 1
- 125000002228 disulfide group Chemical group 0.000 claims 1
- 239000002158 endotoxin Substances 0.000 claims 1
- 239000004052 folic acid antagonist Substances 0.000 claims 1
- 125000005179 haloacetyl group Chemical group 0.000 claims 1
- 229940125396 insulin Drugs 0.000 claims 1
- 229940047124 interferons Drugs 0.000 claims 1
- PGLTVOMIXTUURA-UHFFFAOYSA-N iodoacetamide Chemical compound NC(=O)CI PGLTVOMIXTUURA-UHFFFAOYSA-N 0.000 claims 1
- 239000003446 ligand Substances 0.000 claims 1
- 125000005439 maleimidyl group Chemical group C1(C=CC(N1*)=O)=O 0.000 claims 1
- WKPWGQKGSOKKOO-RSFHAFMBSA-N maytansine Chemical compound CO[C@@H]([C@@]1(O)C[C@](OC(=O)N1)([C@H]([C@@H]1O[C@@]1(C)[C@@H](OC(=O)[C@H](C)N(C)C(C)=O)CC(=O)N1C)C)[H])\C=C\C=C(C)\CC2=CC(OC)=C(Cl)C1=C2 WKPWGQKGSOKKOO-RSFHAFMBSA-N 0.000 claims 1
- 229920000889 poly(m-phenylene isophthalamide) Polymers 0.000 claims 1
- 238000000746 purification Methods 0.000 claims 1
- 229960004641 rituximab Drugs 0.000 claims 1
- 229950008684 sibrotuzumab Drugs 0.000 claims 1
- 125000003396 thiol group Chemical group [H]S* 0.000 claims 1
- 239000012581 transferrin Substances 0.000 claims 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
- C07K16/065—Purification, fragmentation
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
- A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
- A61K47/68—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment
- A61K47/6801—Drug-antibody or immunoglobulin conjugates defined by the pharmacologically or therapeutically active agent
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
- A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
- A61K47/68—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment
- A61K47/6835—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment the modifying agent being an antibody or an immunoglobulin bearing at least one antigen-binding site
- A61K47/6849—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment the modifying agent being an antibody or an immunoglobulin bearing at least one antigen-binding site the antibody targeting a receptor, a cell surface antigen or a cell surface determinant
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
- C07K16/2863—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for growth factors, growth regulators
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
- C07K16/2878—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the NGF-receptor/TNF-receptor superfamily, e.g. CD27, CD30, CD40, CD95
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
- C07K16/2896—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against molecules with a "CD"-designation, not provided for elsewhere
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/32—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/40—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against enzymes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
- C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Epidemiology (AREA)
- Oncology (AREA)
- Cell Biology (AREA)
- Peptides Or Proteins (AREA)
- Medicinal Preparation (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261709871P | 2012-10-04 | 2012-10-04 | |
| US61/709,871 | 2012-10-04 | ||
| PCT/US2013/063503 WO2014055893A1 (en) | 2012-10-04 | 2013-10-04 | Use of an ion exchange membrane to remove impurities from cell-binding agent cytotoxic agent conjugates |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| RU2015116883A true RU2015116883A (ru) | 2016-11-27 |
Family
ID=50435480
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2015116883A RU2015116883A (ru) | 2012-10-04 | 2013-10-04 | Применение ионообменной мембраны для удаления примесей из конъюгатов клеточносвязывающего агента с цитотоксическим агентом |
Country Status (13)
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2399609B1 (en) | 2005-08-24 | 2015-03-18 | ImmunoGen, Inc. | Process for preparing maytansinoid antibody conjugates |
| AU2010256659B2 (en) | 2009-06-03 | 2015-01-29 | Immunogen, Inc. | Conjugation methods |
| CN107537040A (zh) | 2011-03-29 | 2018-01-05 | 伊缪诺金公司 | 通过一步法制备类美登素抗体缀合物 |
| CN105209592A (zh) * | 2012-10-04 | 2015-12-30 | 伊缪诺金公司 | 使用pvdf膜纯化细胞结合剂细胞毒性剂缀合物 |
| GB201616758D0 (en) * | 2016-10-03 | 2016-11-16 | Ge Healthcare Bio Sciences Ab | Novel chromatography media |
| CN108549763B (zh) * | 2018-04-09 | 2021-06-01 | 电子科技大学 | 一种用于离子推进器数值模拟的电荷交换碰撞mcc方法 |
| CN118290513B (zh) * | 2024-06-05 | 2025-03-14 | 东曜药业有限公司 | 一种去除抗体偶联药物中游离小分子的阴离子柱层析方法及抗体偶联药物 |
| CN118290514B (zh) * | 2024-06-05 | 2025-04-04 | 东曜药业有限公司 | 基于阴离子膜的抗体偶联药物的纯化方法及抗体偶联药物 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050031627A1 (en) * | 2002-01-03 | 2005-02-10 | Mazzola Gregory J | Methods for preparing immunoconjugates |
| US20090068178A1 (en) * | 2002-05-08 | 2009-03-12 | Genentech, Inc. | Compositions and Methods for the Treatment of Tumor of Hematopoietic Origin |
| US20110166319A1 (en) * | 2005-02-11 | 2011-07-07 | Immunogen, Inc. | Process for preparing purified drug conjugates |
| EP2468304A3 (en) * | 2005-02-11 | 2012-09-26 | ImmunoGen, Inc. | Process for preparing stable drug conjugates |
| EP2399609B1 (en) * | 2005-08-24 | 2015-03-18 | ImmunoGen, Inc. | Process for preparing maytansinoid antibody conjugates |
| AU2010256659B2 (en) * | 2009-06-03 | 2015-01-29 | Immunogen, Inc. | Conjugation methods |
| CN107537040A (zh) * | 2011-03-29 | 2018-01-05 | 伊缪诺金公司 | 通过一步法制备类美登素抗体缀合物 |
-
2013
- 2013-10-04 SG SG11201502430QA patent/SG11201502430QA/en unknown
- 2013-10-04 EP EP13843881.7A patent/EP2903450A4/en not_active Withdrawn
- 2013-10-04 JP JP2015535827A patent/JP2015535215A/ja active Pending
- 2013-10-04 CN CN201380061514.1A patent/CN105208876A/zh active Pending
- 2013-10-04 IN IN3203DEN2015 patent/IN2015DN03203A/en unknown
- 2013-10-04 AU AU2013326897A patent/AU2013326897A1/en not_active Abandoned
- 2013-10-04 WO PCT/US2013/063503 patent/WO2014055893A1/en active Application Filing
- 2013-10-04 RU RU2015116883A patent/RU2015116883A/ru not_active Application Discontinuation
- 2013-10-04 HK HK16101358.6A patent/HK1213148A1/zh unknown
- 2013-10-04 MX MX2015004258A patent/MX2015004258A/es unknown
- 2013-10-04 US US14/430,744 patent/US20150307596A1/en not_active Abandoned
- 2013-10-04 CA CA 2886996 patent/CA2886996A1/en not_active Abandoned
-
2015
- 2015-04-02 IL IL238111A patent/IL238111A0/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| MX2015004258A (es) | 2015-09-25 |
| WO2014055893A1 (en) | 2014-04-10 |
| SG11201502430QA (en) | 2015-04-29 |
| HK1213148A1 (zh) | 2016-06-30 |
| IN2015DN03203A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 2015-10-02 |
| JP2015535215A (ja) | 2015-12-10 |
| CA2886996A1 (en) | 2014-04-10 |
| US20150307596A1 (en) | 2015-10-29 |
| IL238111A0 (en) | 2015-05-31 |
| AU2013326897A1 (en) | 2015-05-07 |
| EP2903450A1 (en) | 2015-08-12 |
| EP2903450A4 (en) | 2016-05-11 |
| CN105208876A (zh) | 2015-12-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| RU2015116882A (ru) | Использование пвдф-мембраны для очистки конъюгатов клеточно-связывающий агент - цитотоксический агент | |
| RU2015116883A (ru) | Применение ионообменной мембраны для удаления примесей из конъюгатов клеточносвязывающего агента с цитотоксическим агентом | |
| JP7269210B2 (ja) | １段階の工程によるマイタンシノイド－抗体複合体の調製 | |
| JP2016502501A5 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | ||
| JP5350792B2 (ja) | メイタンシノイド（ｍａｙｔａｎｓｉｎｏｉｄ）抗体複合体の調製方法 | |
| JP2009506032A5 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | ||
| HRP20190898T1 (hr) | Postupci konjugacije | |
| KR20140019415A (ko) | 향상된 균질성의 접합체를 제조하기 위한 방법 | |
| WO2012135517A2 (en) | Preparation of maytansinoid antibody conjugates by a one-step process | |
| EP2790724A1 (en) | Use of n-hydroxysuccinimide to improve conjugate stability | |
| HK40014867A (en) | Preparation of maytansinoid antibody conjugates by a one-step process | |
| HK1194700A (en) | Preparation of maytansinoid antibody conjugates by a one-step process | |
| HK1194700B (en) | Preparation of maytansinoid antibody conjugates by a one-step process | |
| NZ712414B2 (en) | Preparation of antibody maytansinoid conjugates | |
| NZ616509B2 (en) | Preparation of maytansinoid antibody conjugates by a one-step process |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FA93 | Acknowledgement of application withdrawn (no request for examination) |
Effective date: 20161005 |