RU2014127477A

RU2014127477A - METHOD FOR BIOMATERIAL CONSERVATION

Info

Publication number: RU2014127477A
Application number: RU2014127477A
Authority: RU
Inventors: Александр Игоревич Пономарев
Original assignee: Общество с ограниченной ответственностью "ИнПрес"
Priority date: 2014-07-04
Filing date: 2014-07-04
Publication date: 2016-02-10
Also published as: RU2577996C2

Abstract

1. Способ консервации биоматериала, включающий размещение биоматериала в стерильной емкости, удаление воздуха из емкости и ее последующую герметизацию, обработку биоматериала в закрытой среде в емкости со смотровым окном ксеноном и последующее охлаждение биоматериала при постоянном визуальном мониторинге состояния биоматериала посредством смотрового окна, отличающийся тем, что обработку биоматериала производят ксеноном 90-99,999% степенью чистоты до величины давления в 7-9 атм., а последующее охлаждение биоматериала ведут до температуры не более +5°C, но не менее -10°C.2. Способ по п. 1, отличающийся тем, что обработку биоматериала производят в закрытой среде ксеноном 99,999% степенью чистоты.3. Способ по п. 1, отличающийся тем, что удаление воздуха из емкости осуществляют путем продувки емкости ксеноном 90-99,999% степенью чистоты, а именно производят закачку ксенона 90-99,999% степенью чистоты, создавая давление в емкости, равное 1-2 атм., и последующий выпуск газов из емкости до давления в 0 атм.1. A method of preserving biomaterial, including placing biomaterial in a sterile container, removing air from the container and its subsequent sealing, processing the biomaterial in a closed environment in a container with an xenon inspection window and subsequent cooling of the biomaterial with constant visual monitoring of the state of the biomaterial through the viewing window, characterized in that the biomaterial is processed with xenon of 90-99.999% purity to a pressure of 7-9 atm, and the subsequent cooling of the biomaterial is carried out to a temperature e a + 5 ° C, but not less than -10 ° C.2. The method according to claim 1, characterized in that the processing of the biomaterial is carried out in a closed environment with xenon 99.999% degree of purity. The method according to p. 1, characterized in that the air is removed from the tank by purging the tank with xenon 90-99.999% purity, namely, they inject xenon 90-99.999% purity, creating a pressure in the tank equal to 1-2 atm., and the subsequent release of gases from the tank to a pressure of 0 atm.

Claims

1. A method of preserving biomaterial, including placing biomaterial in a sterile container, removing air from the container and its subsequent sealing, processing the biomaterial in a closed environment in a container with an xenon inspection window and subsequent cooling of the biomaterial with constant visual monitoring of the state of the biomaterial through the viewing window, characterized in that the biomaterial is processed with xenon of 90-99.999% purity to a pressure of 7-9 atm, and the subsequent cooling of the biomaterial is carried out to a temperature e a + 5 ° C, but not less than -10 ° C.

2. The method according to p. 1, characterized in that the processing of the biomaterial is carried out in a closed environment with xenon 99.999% degree of purity.

3. The method according to p. 1, characterized in that the air is removed from the tank by purging the tank with xenon 90-99.999% purity, namely, they inject xenon 90-99.999% purity, creating a pressure in the tank equal to 1-2 atm ., and the subsequent release of gases from the tank to a pressure of 0 atm.