RU2014113206A - METHOD FOR FREEZING BIOLOGICAL OBJECTS USING XENON AS A CLATRATE-FORMING AGENT AND CARRIER FOR ITS IMPLEMENTATION - Google Patents
METHOD FOR FREEZING BIOLOGICAL OBJECTS USING XENON AS A CLATRATE-FORMING AGENT AND CARRIER FOR ITS IMPLEMENTATION Download PDFInfo
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- RU2014113206A RU2014113206A RU2014113206/15A RU2014113206A RU2014113206A RU 2014113206 A RU2014113206 A RU 2014113206A RU 2014113206/15 A RU2014113206/15 A RU 2014113206/15A RU 2014113206 A RU2014113206 A RU 2014113206A RU 2014113206 A RU2014113206 A RU 2014113206A
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- carrier
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- freezing
- forming agent
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- Agricultural Chemicals And Associated Chemicals (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
Способ замораживания биологических объектов с использованием ксенона в качестве клатратообразующего агента и носитель для его осуществления, включающий замораживание образца в атмосфере ксенона, отличающийся тем, что для его осуществления используют носитель, состоящий из герметично закрывающейся камеры с ниппелем, в которой размещают биологический объект, на него подают ксенон 99-99,9999% степени чистоты под давлением, достаточным для формирования гидратов ксенона при 0 градусов Цельсия, а затем постепенным охлаждением носителя до желаемой температуры хранения вплоть до -196 градусов Цельсия, размораживание в данной системе осуществляется при выдерживании носителя с находящимся внутри биологическим объектом при комнатной температуре в течение 2-20 минут с последующим вскрытием носителя, сбросом давления в системе и извлечением материала.A method of freezing biological objects using xenon as a clathrate-forming agent and a carrier for its implementation, comprising freezing a sample in xenon atmosphere, characterized in that for its implementation a carrier is used, consisting of a hermetically sealed chamber with a nipple in which the biological object is placed on it supply xenon 99-99.9999% degree of purity under pressure sufficient to form xenon hydrates at 0 degrees Celsius, and then gradually cooling the carrier to the desired My storage temperature is up to -196 degrees Celsius, thawing in this system is carried out by holding the carrier with the biological object inside at room temperature for 2-20 minutes, followed by opening the carrier, depressurizing the system and removing the material.
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Application Number | Priority Date | Filing Date | Title |
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RU2014113206/15A RU2014113206A (en) | 2014-04-05 | 2014-04-05 | METHOD FOR FREEZING BIOLOGICAL OBJECTS USING XENON AS A CLATRATE-FORMING AGENT AND CARRIER FOR ITS IMPLEMENTATION |
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RU2014113206/15A RU2014113206A (en) | 2014-04-05 | 2014-04-05 | METHOD FOR FREEZING BIOLOGICAL OBJECTS USING XENON AS A CLATRATE-FORMING AGENT AND CARRIER FOR ITS IMPLEMENTATION |
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RU2014113206A true RU2014113206A (en) | 2015-10-10 |
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RU2014113206/15A RU2014113206A (en) | 2014-04-05 | 2014-04-05 | METHOD FOR FREEZING BIOLOGICAL OBJECTS USING XENON AS A CLATRATE-FORMING AGENT AND CARRIER FOR ITS IMPLEMENTATION |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
RU2706424C1 (en) * | 2018-07-24 | 2019-11-19 | Жовнерчук Инна Юрьевна | Method of introducing xenon into the human body during xenon therapy |
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2014
- 2014-04-05 RU RU2014113206/15A patent/RU2014113206A/en not_active Application Discontinuation
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
RU2706424C1 (en) * | 2018-07-24 | 2019-11-19 | Жовнерчук Инна Юрьевна | Method of introducing xenon into the human body during xenon therapy |
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Legal Events
Date | Code | Title | Description |
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FA92 | Acknowledgement of application withdrawn (lack of supplementary materials submitted) |
Effective date: 20151123 |