RU2014127426A - METHOD FOR DETERMINING PROPIONIBACTERIUM AND STAPHYLOCOCCUS BACTERIA IN BIOLOGICAL MATERIAL - Google Patents

METHOD FOR DETERMINING PROPIONIBACTERIUM AND STAPHYLOCOCCUS BACTERIA IN BIOLOGICAL MATERIAL Download PDF

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Publication number
RU2014127426A
RU2014127426A RU2014127426/10A RU2014127426A RU2014127426A RU 2014127426 A RU2014127426 A RU 2014127426A RU 2014127426/10 A RU2014127426/10 A RU 2014127426/10A RU 2014127426 A RU2014127426 A RU 2014127426A RU 2014127426 A RU2014127426 A RU 2014127426A
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RU
Russia
Prior art keywords
biological material
propionibacterium
determining
pcr
staphylococcus bacteria
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RU2014127426/10A
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Russian (ru)
Inventor
Диляра Магсумовна Шарифуллина
Андрей Петрович Ложкин
Айрат Мансурович Зиганшин
Рустем Наилевич Хайруллин
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Общество с ограниченной ответственностью "АТЕРО" (сокращ. ООО "АТЕРО")
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Priority to RU2014127426/10A priority Critical patent/RU2014127426A/en
Publication of RU2014127426A publication Critical patent/RU2014127426A/en

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Abstract

1. Способ определения бактерий родов Propionibacterium и Staphylococcus в биологическом материале, заключающийся в выделении, очищении тотальной бактериальной ДНК, выполнении полимеразной цепной реакции (ПЦР) в режиме реального времени.2. Способ по п. 1, отличающийся тем, что в качестве матрицы для ПЦР используют тотальную бактериальную РНК.1. A method for determining bacteria of the genera Propionibacterium and Staphylococcus in biological material, which consists in the isolation, purification of total bacterial DNA, and the execution of polymerase chain reaction (PCR) in real time. The method according to claim 1, characterized in that the total bacterial RNA is used as a template for PCR.

Claims (2)

1. Способ определения бактерий родов Propionibacterium и Staphylococcus в биологическом материале, заключающийся в выделении, очищении тотальной бактериальной ДНК, выполнении полимеразной цепной реакции (ПЦР) в режиме реального времени.1. A method for determining bacteria of the genera Propionibacterium and Staphylococcus in biological material, which consists in the isolation, purification of total bacterial DNA, the implementation of polymerase chain reaction (PCR) in real time. 2. Способ по п. 1, отличающийся тем, что в качестве матрицы для ПЦР используют тотальную бактериальную РНК.2. The method according to p. 1, characterized in that the total bacterial RNA is used as a template for PCR.
RU2014127426/10A 2014-06-23 2014-06-23 METHOD FOR DETERMINING PROPIONIBACTERIUM AND STAPHYLOCOCCUS BACTERIA IN BIOLOGICAL MATERIAL RU2014127426A (en)

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RU2014127426/10A RU2014127426A (en) 2014-06-23 2014-06-23 METHOD FOR DETERMINING PROPIONIBACTERIUM AND STAPHYLOCOCCUS BACTERIA IN BIOLOGICAL MATERIAL

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RU2014127426/10A RU2014127426A (en) 2014-06-23 2014-06-23 METHOD FOR DETERMINING PROPIONIBACTERIUM AND STAPHYLOCOCCUS BACTERIA IN BIOLOGICAL MATERIAL

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RU2014127426A true RU2014127426A (en) 2015-12-27

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2630673C1 (en) * 2016-12-19 2017-09-11 Федеральное государственное бюджетное образовательное учреждение высшего образования "Московский государственный университет имени М.В. Ломоносова" (МГУ) Method for determination of biomaterials bacterial contamination

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2630673C1 (en) * 2016-12-19 2017-09-11 Федеральное государственное бюджетное образовательное учреждение высшего образования "Московский государственный университет имени М.В. Ломоносова" (МГУ) Method for determination of biomaterials bacterial contamination

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Effective date: 20151116