RU2012158198A - METHOD OF TRANSFECTION USING A CATION PEPTIDE CONJUGATE WITH CXCR4 RECEPTOR LIGAND - Google Patents
METHOD OF TRANSFECTION USING A CATION PEPTIDE CONJUGATE WITH CXCR4 RECEPTOR LIGAND Download PDFInfo
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- RU2012158198A RU2012158198A RU2012158198/10A RU2012158198A RU2012158198A RU 2012158198 A RU2012158198 A RU 2012158198A RU 2012158198/10 A RU2012158198/10 A RU 2012158198/10A RU 2012158198 A RU2012158198 A RU 2012158198A RU 2012158198 A RU2012158198 A RU 2012158198A
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- cxcr4 receptor
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Abstract
1. Способ трансфекции с использованием конъюгата катионного пептида с лигандом рецептора CXCR4, применяемого в составе носителя генетических конструкций для направленной доставки нуклеиновых кислот в клетки на поверхности которых представлен рецептор CXCR4, отличающийся тем, что в состав носителя включают- последовательность-лиганд к рецептору CXCR4 на поверхности клеток - KPVSLSYRSPSRFFESH;- линкерный участок из двух молекул ε-аминогексановой кислоты, соединяющего последовательность-лиганд с последовательностью для компактизации генетической конструкции;- последовательность, обеспечивающую компактизацию нуклеиновых кислот и выход комплексов из эндосом - CHRRRRRRHC.2. Способ по п.1, отличающийся тем, что в качестве генетического материала для носителей используют плазмидную ДНК.3. Способ по п.1, отличающийся тем, что в качестве генетического материала используют олигонуклеотидные последовательности РНК и ДНК, интерферирующие РНК.1. The method of transfection using a conjugate of a cationic peptide with a ligand of the CXCR4 receptor, used as part of a carrier of genetic constructs for targeted delivery of nucleic acids to cells on the surface of which a CXCR4 receptor is presented, characterized in that the carrier composition includes a ligand sequence for the CXCR4 receptor on cell surfaces - KPVSLSYRSPSRFFESH; - linker site of two ε-aminohexanoic acid molecules connecting the ligand sequence to the sequence for compaction of the genetic the sequence providing nucleic acid compaction and exit of complexes from endosomes - CHRRRRRRHC.2. The method according to claim 1, characterized in that plasmid DNA is used as the genetic material for the carriers. The method according to claim 1, characterized in that the oligonucleotide sequences of RNA and DNA interfering with RNA are used as genetic material.
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RU2012158198/10A RU2522810C1 (en) | 2012-12-21 | 2012-12-21 | Carrier for targeted delivery of nucleic acids to cells expressing receptor cxcr4 |
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RU2012158198/10A RU2522810C1 (en) | 2012-12-21 | 2012-12-21 | Carrier for targeted delivery of nucleic acids to cells expressing receptor cxcr4 |
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RU2012158198A true RU2012158198A (en) | 2014-06-27 |
RU2522810C1 RU2522810C1 (en) | 2014-07-20 |
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RU2012158198/10A RU2522810C1 (en) | 2012-12-21 | 2012-12-21 | Carrier for targeted delivery of nucleic acids to cells expressing receptor cxcr4 |
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Families Citing this family (2)
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RU2572575C1 (en) * | 2014-10-31 | 2016-01-20 | Федеральное государственное бюджетное учреждение "Государственный научный центр "Институт иммунологии" Федерального медико-биологического агентства России (ФГБУ "ГНЦ Институт иммунологии" ФМБА России) | Mean for intracellular delivery of nucleic acids to cells of mammals |
WO2021020462A1 (en) * | 2019-07-29 | 2021-02-04 | 国立大学法人京都大学 | Intracellular delivery peptide |
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AU2002225187A1 (en) * | 2001-01-22 | 2002-07-30 | Sangamo Biosciences, Inc. | Zinc finger polypeptides and their use |
US20070111201A1 (en) * | 2001-04-30 | 2007-05-17 | Benjamin Doranz | Reverse transfection of cell arrays for structural and functional analyses of proteins |
RU2408607C2 (en) * | 2008-12-25 | 2011-01-10 | ООО "ДжинАрт" | Method for directed delivery of dna into tumor and stem cells |
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Effective date: 20141222 |