RU2012101021A

RU2012101021A - AGRICULTURAL PLANT GROWTH biostimulator from chitin of crustaceans and a method of producing a growth biostimulant of agricultural plants from crustacean chitin

Info

Publication number: RU2012101021A
Application number: RU2012101021/13A
Authority: RU
Inventors: Марфуга Дюсембаевна Мукатова; Тамара Васильевна Боева; Шамиль Байрамбекович Байрамбеков; Наталья Александровна Киричко; Ренат Рахметуллаевич Утеушев
Priority date: 2012-01-11
Filing date: 2012-01-11
Publication date: 2013-07-20
Also published as: RU2504953C2

Abstract

1. Биостимулятор роста сельскохозяйственных растений, характеризующийся тем, что он представляет собой раствор, содержащий мас.%:2. Способ получения биостимулятора роста сельскохозяйственных растений из хитина ракообразных, характеризующийся тем, что хитин деацетилируют 45%-ным раствором гидроксида натрия при гидромодуле 1:10, температуре 90-100°C, продолжительности 3-4 ч, получают высокомолекулярный хитозан, промывают его водой до pH=7, сушат конвективным способом при температуре 35-40°C, проводят гидролиз ферментным препаратом папаином концентрацией 0,3-0,5% в течение 5-6 ч при температуре 45-50°C, осаждают полученный низкомолекулярный хитозан с молекулярной массой 10-25 кДа 10%-ным раствором гидроксида натрия, промывают водопроводной водой до pH 8 и сушат при температуре 35-40°C, растворяют 0,5-3% хитозана в 0,5-1%-ном растворе молочной кислоты взятом в количестве 10-90% с добавлением молочной сыворотки взятой в количестве 10-90% от общей массы.3. Способ по п.2, отличающийся тем, что гидролиз высокомолекулярного хитозана проводят ферментом протосубтилином концентрацией 2-3% в течение 5-7 ч при температуре 40-45°C.1. Biostimulant growth of agricultural plants, characterized in that it is a solution containing wt.%: 2. A method of producing a biostimulator of growth of agricultural plants from crustacean chitin, characterized in that the chitin is deacetylated with a 45% sodium hydroxide solution at a hydromodule of 1:10, a temperature of 90-100 ° C, lasting 3-4 hours, a high molecular weight chitosan is obtained, washed with water until pH = 7, dried convectively at a temperature of 35-40 ° C, hydrolysis with an enzyme preparation of papain at a concentration of 0.3-0.5% for 5-6 hours at a temperature of 45-50 ° C, precipitated obtained low molecular weight chitosan with a molecular weight 10-25 kDa 10% p a solution of sodium hydroxide, washed with tap water to a pH of 8 and dried at a temperature of 35-40 ° C, dissolve 0.5-3% chitosan in a 0.5-1% solution of lactic acid taken in an amount of 10-90% with the addition of lactic serum taken in an amount of 10-90% of the total mass. 3. The method according to claim 2, characterized in that the hydrolysis of high molecular weight chitosan is carried out by a protosubtilin enzyme with a concentration of 2-3% for 5-7 hours at a temperature of 40-45 ° C.

Claims

1. Biostimulant growth of agricultural plants, characterized in that it is a solution containing wt.%:

low molecular weight chitosan with a molecular weight of 10-25 kDa 0.5-3 0.5-1% aqueous solution of lactic acid 10-90 milk serum 10-90

2. A method of producing a biostimulator of growth of agricultural plants from crustacean chitin, characterized in that the chitin is deacetylated with a 45% sodium hydroxide solution at a hydromodule of 1:10, a temperature of 90-100 ° C, a duration of 3-4 hours, a high molecular weight chitosan is obtained, it is washed water to pH = 7, dried convectively at a temperature of 35-40 ° C, hydrolysis with an enzyme preparation of papain at a concentration of 0.3-0.5% for 5-6 hours at a temperature of 45-50 ° C, precipitated obtained low molecular weight chitosan with molecular weight 10-25 kDa 10% sodium hydroxide solution, washed with tap water to pH 8 and dried at a temperature of 35-40 ° C, dissolved with 0.5-3% chitosan in a 0.5-1% solution of lactic acid taken in an amount of 10-90% with the addition of lactic serum taken in an amount of 10-90% of the total mass.

3. The method according to claim 2, characterized in that the hydrolysis of high molecular weight chitosan is carried out by a protosubtilin enzyme with a concentration of 2-3% for 5-7 hours at a temperature of 40-45 ° C.