RU2011115535A - COMPOSITION SIMULATING IN-JOINT LIQUID AND METHOD FOR PRODUCING AN ADDITIVE TO IT - Google Patents

COMPOSITION SIMULATING IN-JOINT LIQUID AND METHOD FOR PRODUCING AN ADDITIVE TO IT Download PDF

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RU2011115535A
RU2011115535A RU2011115535/15A RU2011115535A RU2011115535A RU 2011115535 A RU2011115535 A RU 2011115535A RU 2011115535/15 A RU2011115535/15 A RU 2011115535/15A RU 2011115535 A RU2011115535 A RU 2011115535A RU 2011115535 A RU2011115535 A RU 2011115535A
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solution
composition
deionized water
polyvinylpyrrolidone
sodium salt
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RU2011115535/15A
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RU2473352C2 (en
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Умберто Орацио Джузеппе Мауджери (IT)
Умберто Орацио Джузеппе Мауджери
Вячеслав Иванович Беклемышев (RU)
Вячеслав Иванович Беклемышев
Игорь Иванович Махонин (RU)
Игорь Иванович Махонин
Ара Аршавирович Абрамян (RU)
Ара Аршавирович Абрамян
Владимир Александрович Солодовников (RU)
Владимир Александрович Солодовников
Константин Витальевич Филиппов (RU)
Константин Витальевич Филиппов
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Закрытое акционерное общество "Институт прикладной нанотехнологии" (RU)
Закрытое акционерное общество "Институт прикладной нанотехнологии"
Фонд Сальваторе Мауджери Клиника Труда и Реабилитации (IT)
Фонд Сальваторе Мауджери Клиника Труда и Реабилитации
СИБ Лэборетрис Лимитед (MT)
СИБ Лэборетрис Лимитед
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Priority to RU2011115535/15A priority Critical patent/RU2473352C2/en
Priority to PCT/EP2012/057257 priority patent/WO2012143502A1/en
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y40/00—Manufacture or treatment of nanostructures
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
    • A61K31/726—Glycosaminoglycans, i.e. mucopolysaccharides
    • A61K31/728—Hyaluronic acid
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/74—Synthetic polymeric materials
    • A61K31/785—Polymers containing nitrogen
    • A61K31/787—Polymers containing nitrogen containing heterocyclic rings having nitrogen as a ring hetero atom
    • A61K31/79—Polymers of vinyl pyrrolidone
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K33/00—Medicinal preparations containing inorganic active ingredients
    • A61K33/24—Heavy metals; Compounds thereof
    • A61K33/38—Silver; Compounds thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/02—Inorganic compounds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
    • A61K47/32—Macromolecular compounds obtained by reactions only involving carbon-to-carbon unsaturated bonds, e.g. carbomers, poly(meth)acrylates, or polyvinyl pyrrolidone
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
    • A61K47/36—Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/0012—Galenical forms characterised by the site of application
    • A61K9/0019—Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A61P19/02—Drugs for skeletal disorders for joint disorders, e.g. arthritis, arthrosis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Epidemiology (AREA)
  • Engineering & Computer Science (AREA)
  • Nanotechnology (AREA)
  • Inorganic Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Dermatology (AREA)
  • Molecular Biology (AREA)
  • Manufacturing & Machinery (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Condensed Matter Physics & Semiconductors (AREA)
  • General Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Medical Informatics (AREA)
  • Rheumatology (AREA)
  • Physical Education & Sports Medicine (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Organic Chemistry (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicinal Preparation (AREA)
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Abstract

1. Состав, имитирующий внутрисуставную жидкость, содержащий 15%-й водный раствор среднемолекулярного поливинилпирролидона, при этом раствор содержит (0,5-1,5)·10-3 мас.% добавки с антимикробными свойствами на основе наночастиц кремния, модифицированных серебром, при размере наночастиц 10-40 нм и количестве серебра в добавке 0,8-1,1 мас.%. ! 2. Состав, имитирующий внутрисуставную жидкость по п.1, отличающийся тем, что состав дополнительно содержит 0,05-2,5% раствора натриевой соли гиалуроновой кислоты в деионизованной воде при весовом соотношении: раствор поливинилпирролидона:раствор натриевой соль гиалуроновой кислоты, как 1:(0,2-0,3). ! 3. Состав, имитирующий внутрисуставную жидкость по п.2, отличающийся тем, что используют полученную путем биологической ферментации натриевую соль гиалуроновой кислоты с молекулярной массой 0,5-1,8 МДа. ! 4. Состав, имитирующий внутрисуставную жидкость по п.1, отличающийся тем, что используют 15%-й водный раствор поливинилпирролидона с молекулярной массой 35000±5000 Да. ! 5. Способ получения добавки для состава, заключающийся в получения наночастиц кремния размером 10-40 нм при лазерном пиролизе моносилана (SiH4), при этом поверхность наночастиц кремния модифицируют путем обработки их в 2-5%-м растворе нитрата серебра в деионизованной воде при весовом соотношении: раствор:наночастицы, как 1:0,02, с последующим центрифугированием раствора, многократной промывкой осадка в деионизованной воде с центрифугированием на каждом этапе промывки. ! 6. Способ по п.5, отличающийся тем, что многократную промывку осадка в деионизованной воде осуществляют при исходном удельном сопротивлении воды не ниже 10 МОм·см и до достижения е 1. A composition that mimics the intraarticular fluid containing a 15% aqueous solution of medium molecular weight polyvinylpyrrolidone, the solution containing (0.5-1.5) · 10-3 wt.% Additives with antimicrobial properties based on silver-modified silicon nanoparticles, when the size of the nanoparticles is 10-40 nm and the amount of silver in the additive is 0.8-1.1 wt.%. ! 2. A composition that mimics the intra-articular fluid according to claim 1, characterized in that the composition further comprises 0.05-2.5% solution of hyaluronic acid sodium salt in deionized water at a weight ratio: polyvinylpyrrolidone solution: hyaluronic acid sodium salt solution, as 1 : (0.2-0.3). ! 3. A composition that simulates the intraarticular fluid according to claim 2, characterized in that the sodium salt of hyaluronic acid obtained by biological fermentation with a molecular weight of 0.5-1.8 MDa is used. ! 4. A composition that mimics the intraarticular fluid according to claim 1, characterized in that a 15% aqueous solution of polyvinylpyrrolidone with a molecular weight of 35,000 ± 5,000 Da is used. ! 5. The method of producing additives for the composition, which consists in obtaining silicon nanoparticles with a size of 10-40 nm by laser pyrolysis of monosilane (SiH4), while the surface of silicon nanoparticles is modified by processing them in a 2-5% solution of silver nitrate in deionized water at weight ratio: solution: nanoparticles, as 1: 0.02, followed by centrifugation of the solution, repeated washing of the precipitate in deionized water with centrifugation at each washing stage. ! 6. The method according to claim 5, characterized in that the multiple washing of the precipitate in deionized water is carried out at an initial specific water resistance of at least 10 MOhm · cm and until reaching e

Claims (7)

1. Состав, имитирующий внутрисуставную жидкость, содержащий 15%-й водный раствор среднемолекулярного поливинилпирролидона, при этом раствор содержит (0,5-1,5)·10-3 мас.% добавки с антимикробными свойствами на основе наночастиц кремния, модифицированных серебром, при размере наночастиц 10-40 нм и количестве серебра в добавке 0,8-1,1 мас.%.1. The composition that simulates the intraarticular fluid containing a 15% aqueous solution of medium molecular weight polyvinylpyrrolidone, the solution contains (0.5-1.5) · 10 -3 wt.% Additives with antimicrobial properties based on silver-modified silicon nanoparticles, when the nanoparticle size is 10-40 nm and the amount of silver in the additive is 0.8-1.1 wt.%. 2. Состав, имитирующий внутрисуставную жидкость по п.1, отличающийся тем, что состав дополнительно содержит 0,05-2,5% раствора натриевой соли гиалуроновой кислоты в деионизованной воде при весовом соотношении: раствор поливинилпирролидона:раствор натриевой соль гиалуроновой кислоты, как 1:(0,2-0,3).2. The composition that simulates the intraarticular fluid according to claim 1, characterized in that the composition additionally contains 0.05-2.5% solution of sodium salt of hyaluronic acid in deionized water at a weight ratio of a solution of polyvinylpyrrolidone: a solution of sodium salt of hyaluronic acid, as 1 : (0.2-0.3). 3. Состав, имитирующий внутрисуставную жидкость по п.2, отличающийся тем, что используют полученную путем биологической ферментации натриевую соль гиалуроновой кислоты с молекулярной массой 0,5-1,8 МДа.3. A composition that simulates the intraarticular fluid according to claim 2, characterized in that the sodium salt of hyaluronic acid obtained by biological fermentation with a molecular weight of 0.5-1.8 MDa is used. 4. Состав, имитирующий внутрисуставную жидкость по п.1, отличающийся тем, что используют 15%-й водный раствор поливинилпирролидона с молекулярной массой 35000±5000 Да.4. A composition that mimics the intraarticular fluid according to claim 1, characterized in that a 15% aqueous solution of polyvinylpyrrolidone with a molecular weight of 35,000 ± 5,000 Da is used. 5. Способ получения добавки для состава, заключающийся в получения наночастиц кремния размером 10-40 нм при лазерном пиролизе моносилана (SiH4), при этом поверхность наночастиц кремния модифицируют путем обработки их в 2-5%-м растворе нитрата серебра в деионизованной воде при весовом соотношении: раствор:наночастицы, как 1:0,02, с последующим центрифугированием раствора, многократной промывкой осадка в деионизованной воде с центрифугированием на каждом этапе промывки.5. The method of producing additives for the composition, which consists in obtaining silicon nanoparticles with a size of 10-40 nm by laser pyrolysis of monosilane (SiH 4 ), while the surface of the silicon nanoparticles is modified by processing them in a 2-5% solution of silver nitrate in deionized water at weight ratio: solution: nanoparticles, as 1: 0.02, followed by centrifugation of the solution, repeated washing of the precipitate in deionized water with centrifugation at each washing stage. 6. Способ по п.5, отличающийся тем, что многократную промывку осадка в деионизованной воде осуществляют при исходном удельном сопротивлении воды не ниже 10 МОм·см и до достижения ее удельного сопротивления не ниже 1 МОм·см.6. The method according to claim 5, characterized in that the multiple washing of the precipitate in deionized water is carried out at an initial specific resistivity of water of at least 10 MΩ · cm and until its specific resistance is at least 1 MΩ · cm. 7. Способ по п.5, отличающийся тем, что обработку наночастиц кремния в водном растворе нитрата серебра осуществляют в течение 12-36 ч. 7. The method according to claim 5, characterized in that the processing of silicon nanoparticles in an aqueous solution of silver nitrate is carried out for 12-36 hours
RU2011115535/15A 2011-04-21 2011-04-21 Intra-articular fluid simulator formulation and method for preparing intra-articular fluid additive RU2473352C2 (en)

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RU2011115535/15A RU2473352C2 (en) 2011-04-21 2011-04-21 Intra-articular fluid simulator formulation and method for preparing intra-articular fluid additive
PCT/EP2012/057257 WO2012143502A1 (en) 2011-04-21 2012-04-20 Composition imitating intraarticular liquid

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CN116918831B (en) * 2023-07-25 2024-03-12 山东鑫永恒新材料有限公司 Silver-loaded antibacterial slow-release material and preparation method thereof

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