RU2011109882A - METHOD FOR DIAGNOSTIC OF BLADDER CANCER USING THE ONCOMARKER ATR6V1C1 (OPTIONS) AND KIT FOR ITS IMPLEMENTATION - Google Patents
METHOD FOR DIAGNOSTIC OF BLADDER CANCER USING THE ONCOMARKER ATR6V1C1 (OPTIONS) AND KIT FOR ITS IMPLEMENTATION Download PDFInfo
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- RU2011109882A RU2011109882A RU2011109882/10A RU2011109882A RU2011109882A RU 2011109882 A RU2011109882 A RU 2011109882A RU 2011109882/10 A RU2011109882/10 A RU 2011109882/10A RU 2011109882 A RU2011109882 A RU 2011109882A RU 2011109882 A RU2011109882 A RU 2011109882A
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- RU
- Russia
- Prior art keywords
- atp6v1c1
- diagnosis
- bladder cancer
- real
- time pcr
- Prior art date
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Abstract
1. Способ диагностики рака мочевого пузыря методом ПЦР в режиме реального времени, включающий получение биоматериала и выделение РНК, синтез кДНК на матрице РНК, нормирование концентрации кДНК ATP6V1C1 по контрольному гену, проведение количественной ПЦР-амплификации фрагмента гена ATP6V1C1 и проведение диагностики путем определения количества амплифицированного фрагмента ДНК ATP6V1C1 для образца биоматериала. ! 2. Набор для диагностики рака мочевого пузыря по п.1 методом ПЦР в реальном времени, включающий праймеры с последовательностью SEQ ID NO: 1 и 2 при их молярном соотношении 1:1. ! 3. Способ диагностики рака мочевого пузыря методом иммуноферментного анализа, включающий получение образцов мочи и крови от пациента, выделение смеси белковых компонентов мочи и крови, проведение реакции иммуноферментного анализа с моноклональными и/или поликлональными антителами и/или их фрагментами против рекомбинантного белка ATP6V1C1 и/или его уникальных фрагментов длиной свыше 8 аминокислот и проведение диагностики путем определения содержания белка ATP6V1C1 в исследуемых образцах. 1. A real-time PCR method for the diagnosis of bladder cancer by using real-time PCR, including biomaterial production and RNA isolation, cDNA synthesis on an RNA matrix, normalization of the ATP6V1C1 cDNA concentration by the control gene, quantitative PCR amplification of the ATP6V1C1 gene fragment and diagnosis by determining the amount of amplified ATP6V1C1 DNA fragment for a biomaterial sample. ! 2. The kit for the diagnosis of bladder cancer according to claim 1 by real-time PCR, including primers with the sequence of SEQ ID NO: 1 and 2 with a molar ratio of 1: 1. ! 3. A method for the diagnosis of bladder cancer by enzyme immunoassay, including obtaining urine and blood samples from a patient, isolating a mixture of protein components of urine and blood, conducting an enzyme immunoassay reaction with monoclonal and / or polyclonal antibodies and / or fragments thereof against recombinant protein ATP6V1C1 and / or its unique fragments with a length of over 8 amino acids and conducting diagnostics by determining the content of ATP6V1C1 protein in the studied samples.
Claims (3)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU2011109882/10A RU2469098C2 (en) | 2011-03-16 | 2011-03-16 | Method for estimating clinical effectiveness of human bladder cancer by real-time pcr and kit for implementation thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU2011109882/10A RU2469098C2 (en) | 2011-03-16 | 2011-03-16 | Method for estimating clinical effectiveness of human bladder cancer by real-time pcr and kit for implementation thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RU2011109882A true RU2011109882A (en) | 2012-09-27 |
| RU2469098C2 RU2469098C2 (en) | 2012-12-10 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2011109882/10A RU2469098C2 (en) | 2011-03-16 | 2011-03-16 | Method for estimating clinical effectiveness of human bladder cancer by real-time pcr and kit for implementation thereof |
Country Status (1)
| Country | Link |
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| RU (1) | RU2469098C2 (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2624505C1 (en) * | 2016-06-03 | 2017-07-04 | Федеральное государственное бюджетное учреждение "Ростовский научно-исследовательский онкологический институт" Министерства здравоохранения Российской Федерации | Method for prediction of metastases development in patients with gastric cancer |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007516693A (en) * | 2003-06-09 | 2007-06-28 | ザ・リージェンツ・オブ・ザ・ユニバーシティ・オブ・ミシガン | Compositions and methods for the treatment and diagnosis of cancer |
| US20070099209A1 (en) * | 2005-06-13 | 2007-05-03 | The Regents Of The University Of Michigan | Compositions and methods for treating and diagnosing cancer |
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- 2011-03-16 RU RU2011109882/10A patent/RU2469098C2/en not_active IP Right Cessation
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| Publication number | Publication date |
|---|---|
| RU2469098C2 (en) | 2012-12-10 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MM4A | The patent is invalid due to non-payment of fees |
Effective date: 20160317 |