RU2010127796A

RU2010127796A - ASSOCIATED VACCINE AGAINST SKIN CANDIDIOSIS OF CARNIVORES, METHOD OF MAKING ASSOCIATED VACCINE AGAINST SKIN CANDIDOSIS OF CARNIVORES, METHOD FOR PREVENTION AND THERAPY OF SKIN CANDIDIDOSIS OF CARNIDA

Info

Publication number: RU2010127796A
Application number: RU2010127796/10A
Authority: RU
Inventors: Алексей Михайлович Литвинов (RU); Алексей Михайлович Литвинов; Наталья Александровна Апанасенко (RU); Наталья Александровна Апанасенко
Priority date: 2010-07-07
Filing date: 2010-07-07
Publication date: 2012-01-20
Also published as: RU2445109C1

Abstract

1. Ассоциированная вакцина против кожного кандидоза плотоядных, отличающаяся тем, что в качестве антигенов содержит бластоспоры штаммов С.albicans №95 и С.tropicalis №87, взятые в равных соотношениях (1:1) с конечным количеством 10-40 млн бластоспор в 1 см3 0,8-1%-ного серноватистокислого натрия. ! 2. Способ изготовления ассоциированной вакцины против кожного кандидоза плотоядных, отличающийся тем, что бластоспоры грибов двух видов берут в целом виде, по отдельности гомогенизируют с 0,8-1%-ным раствором серноватистокислого натрия, определяют содержание бластоспор грибов в гомогенатах путем подсчета в камере Горяева, стандартизируют грибные гомогенаты по количеству грибных клеток в единице объема 0,8-1%-ного серноватистокислого натрия, гомогенаты смешивают в соотношении 1:1 до конечного количества 10-40 млн бластоспор в 1 см3, повторно стандартизируют, проводят ступенчатую инактивацию температурой от 45 до 55°С в течение 57-81 ч. ! 3. Способ профилактики и терапии кожного кандидоза плотоядных заключается во внутримышечном введении вакцины сначала в одно, а через 12-16 дней, повторно, в противоположное место в лечебно-профилактических дозах: серебристо-черным лисицам в возрасте от 30 до 45 сут. - 0,3-0,7 см3, старше 45 сут. - 0,8-1,2 см3; кошкам и собакам старше 45 сут. с массой тела до 1 кг - 0,3-0,7 см3, от 1 до 35 кг - 0,8-1,2 см3, от 35 кг и более - 1,3-1,7 см3. 1. The associated vaccine against cutaneous carnivorous candidiasis, characterized in that it contains blastospores of strains of C. albicans No. 95 and C. tropicalis No. 87, taken in equal proportions (1: 1) with a final amount of 10-40 million blastospores in 1 as antigens cm3 of 0.8-1% sodium sulfate. ! 2. A method of manufacturing an associated vaccine against skin candidiasis of carnivores, characterized in that the blastospores of the two species of mushrooms are taken as a whole, individually homogenized with a 0.8-1% solution of sodium sulfate, the content of the blastospores of the mushrooms in the homogenates is determined by counting in a chamber Goryaeva, mushroom homogenates are standardized by the number of mushroom cells per unit volume of 0.8-1% sodium sulfate, homogenates are mixed in a ratio of 1: 1 to a final amount of 10-40 million blastospores in 1 cm3, repeatedly they standardize, carry out stepwise inactivation with a temperature from 45 to 55 ° C for 57-81 hours! 3. The method for the prevention and treatment of cutaneous carnivorous candidiasis consists in the intramuscular injection of the vaccine, first in one, and after 12-16 days, again, in the opposite place in therapeutic doses: silver-black foxes aged 30 to 45 days. - 0.3-0.7 cm3, older than 45 days. - 0.8-1.2 cm3; cats and dogs older than 45 days. with a body weight of up to 1 kg - 0.3-0.7 cm3, from 1 to 35 kg - 0.8-1.2 cm3, from 35 kg and more - 1.3-1.7 cm3.

Claims

1. The associated vaccine against cutaneous carnivorous candidiasis, characterized in that it contains blastospores of strains of C. albicans No. 95 and C. tropicalis No. 87, taken in equal proportions (1: 1) with a final amount of 10-40 million blastospores in 1 as antigens cm ^{3 of} 0.8-1% sodium sulfate.

2. A method of manufacturing an associated vaccine against skin candidiasis of carnivores, characterized in that the blastospores of the two species of mushrooms are taken as a whole, individually homogenized with a 0.8-1% solution of sodium sulfate, the content of the blastospores of the mushrooms in the homogenates is determined by counting in a chamber Goryaeva, mushroom homogenates are standardized by the number of mushroom cells per unit volume of 0.8-1% sodium sulfate, homogenates are mixed in a ratio of 1: 1 to a final amount of 10-40 million blastospores in 1 cm ³ , repeatedly andartize, carry out stepwise inactivation with a temperature from 45 to 55 ° C for 57-81 hours

3. The method for the prevention and treatment of cutaneous carnivorous candidiasis consists in the intramuscular injection of the vaccine, first in one, and after 12-16 days, again, in the opposite place in therapeutic doses: silver-black foxes aged 30 to 45 days. - 0.3-0.7 cm ³ , older than 45 days. - 0.8-1.2 cm ³ ; cats and dogs older than 45 days. with a body weight of up to 1 kg - 0.3-0.7 cm ³ , from 1 to 35 kg - 0.8-1.2 cm ³ , from 35 kg and more - 1.3-1.7 cm ³ .