RU2010120769A - METHOD FOR PRODUCING DISPOSABLE DISPOSABLE CATION FILMS-COVERINGS OF MALDI TARGETS AND THEIR APPLICATION FOR MASS-SPECTROMETRIC ANALYSIS OF DNA - Google Patents
METHOD FOR PRODUCING DISPOSABLE DISPOSABLE CATION FILMS-COVERINGS OF MALDI TARGETS AND THEIR APPLICATION FOR MASS-SPECTROMETRIC ANALYSIS OF DNA Download PDFInfo
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- RU2010120769A RU2010120769A RU2010120769/10A RU2010120769A RU2010120769A RU 2010120769 A RU2010120769 A RU 2010120769A RU 2010120769/10 A RU2010120769/10 A RU 2010120769/10A RU 2010120769 A RU2010120769 A RU 2010120769A RU 2010120769 A RU2010120769 A RU 2010120769A
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- maldi
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- biomolecules
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- water
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Abstract
1. Способ получения временных водонерастворимых ионообменных покрытий на стандартных стальных МАЛДИ мишенях, а также на стальных, стеклянных, керамических или пластиковых вставках в МАЛДИ мишень, совместимых с выполнением последующего МАЛДИ анализа, заключающийся в том, что поверхность МАЛДИ мишени покрывается водно-органическим раствором ионогенного полимера и нитроцеллюлозы и высушивается, после использования такие покрытия могут быть удалены водно-спиртовыми растворами. ! 2. Способ подготовки олигонуклеотидных проб для МАЛДИ МС анализа, сочетающий операции концентрирования образца и его обессоливания в одном шаге, выполняемом на стандартной МАЛДИ мишени или на вставках для нее, способ заключается в концентрировании биомолекул путем нанесения водных растворов, их содержащих, на подготовленные по п.1 поверхности, после нанесения такого рода смесей через несколько секунд супернатант удаляется и поверхность промывается водой или разбавленным водным аммонийным буфером. ! 3. Способ повышения достоверности и воспроизводимости МАЛДИ анализа биомолекул, сорбированных на подготовленных по п.2 МАЛДИ мишенях, заключающийся в том, что после шага иммобилизации биомолекул интерферирующие примеси, такие как солевые аддукты и поверхностно-активные вещества, удаляются отмывкой поверхности МАЛДИ мишени и на те же точки наносится МАЛДИ матрица, в раствор которой переходят ранее иммобилизованные биомолекулы, после высыхания раствора матрицы МАЛДИ анализ выполняется с этой же подложки. 1. The method of obtaining temporary water-insoluble ion-exchange coatings on standard steel MALDI targets, as well as on steel, glass, ceramic or plastic inserts in the MALDI target, compatible with the subsequent MALDI analysis, which consists in the fact that the surface of the MALDI target is coated with an aqueous-organic solution of ionogenic polymer and nitrocellulose and dried, after use, such coatings can be removed with water-alcohol solutions. ! 2. A method for preparing oligonucleotide samples for MALDI MS analysis, combining the operations of sample concentration and desalination in one step, performed on a standard MALDI target or on inserts for it, the method consists in concentrating biomolecules by applying aqueous solutions containing them to prepared according to .1 of the surface, after applying such mixtures, after a few seconds, the supernatant is removed and the surface is washed with water or diluted aqueous ammonium buffer. ! 3. A way to increase the reliability and reproducibility of MALDI analysis of biomolecules adsorbed on targets prepared according to clause 2 of MALDI, which consists in the fact that after the step of immobilization of biomolecules, interfering impurities, such as salt adducts and surfactants, are removed by washing the surface of the MALDI target and the MALDI matrix is applied to the same points, into the solution of which previously immobilized biomolecules pass, after the MALDI matrix solution has dried, the analysis is performed from the same substrate.
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Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU2010120769/10A RU2475543C2 (en) | 2010-05-25 | 2010-05-25 | Method to produce removable water-insoluble ion-exchange coatings on maldi targets and their application for mass-spectrometric analysis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU2010120769/10A RU2475543C2 (en) | 2010-05-25 | 2010-05-25 | Method to produce removable water-insoluble ion-exchange coatings on maldi targets and their application for mass-spectrometric analysis |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RU2010120769A true RU2010120769A (en) | 2011-11-27 |
| RU2475543C2 RU2475543C2 (en) | 2013-02-20 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2010120769/10A RU2475543C2 (en) | 2010-05-25 | 2010-05-25 | Method to produce removable water-insoluble ion-exchange coatings on maldi targets and their application for mass-spectrometric analysis |
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| RU (1) | RU2475543C2 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111527391A (en) * | 2018-09-11 | 2020-08-11 | 株式会社Lg化学 | Method for preparing test piece for MALDI mass spectrometry using water-insoluble substance and method for quantitatively analyzing water-insoluble substance using MALDI mass spectrometry |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19618032C2 (en) * | 1996-05-04 | 2000-04-13 | Bruker Daltonik Gmbh | Prepared Maldi sample carriers that can be stored |
| RU2236467C1 (en) * | 2003-04-14 | 2004-09-20 | Институт химической биологии и фундаментальной медицины СО РАН | Method for preparing dna-chips |
| WO2005022583A2 (en) * | 2003-08-21 | 2005-03-10 | Applera Corporation | Reduction of matrix interference for maldi mass spectrometry analysis |
| EP1775348A1 (en) * | 2005-10-17 | 2007-04-18 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. | Method of analyzing nucleic acid with mass spectrometry |
| RU2321638C2 (en) * | 2006-05-23 | 2008-04-10 | Закрытое акционерное общество "Молекулярно-медицинские технологии" | Method for preparing multifunctional multichip, multichip for successive or parallel screening biopolymers, method for analysis of biopolymers and set for realization of method |
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2010
- 2010-05-25 RU RU2010120769/10A patent/RU2475543C2/en not_active IP Right Cessation
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111527391A (en) * | 2018-09-11 | 2020-08-11 | 株式会社Lg化学 | Method for preparing test piece for MALDI mass spectrometry using water-insoluble substance and method for quantitatively analyzing water-insoluble substance using MALDI mass spectrometry |
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| Publication number | Publication date |
|---|---|
| RU2475543C2 (en) | 2013-02-20 |
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| MM4A | The patent is invalid due to non-payment of fees |
Effective date: 20121202 |