RU2009145253A - METHOD FOR PRODUCING BIOLOGICAL PREPARATIONS BASED ON ENTOMOPATOGENIC NEMATODES - Google Patents

METHOD FOR PRODUCING BIOLOGICAL PREPARATIONS BASED ON ENTOMOPATOGENIC NEMATODES Download PDF

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Publication number
RU2009145253A
RU2009145253A RU2009145253/10A RU2009145253A RU2009145253A RU 2009145253 A RU2009145253 A RU 2009145253A RU 2009145253/10 A RU2009145253/10 A RU 2009145253/10A RU 2009145253 A RU2009145253 A RU 2009145253A RU 2009145253 A RU2009145253 A RU 2009145253A
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Russia
Prior art keywords
nematodes
nutrient medium
preparations based
biological preparations
autoclaving
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RU2009145253/10A
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Russian (ru)
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RU2444195C2 (en
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Данилов Леонид Григорьевич (RU)
Данилов Леонид Григорьевич
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Priority to RU2009145253/10A priority Critical patent/RU2444195C2/en
Publication of RU2009145253A publication Critical patent/RU2009145253A/en
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Publication of RU2444195C2 publication Critical patent/RU2444195C2/en

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Abstract

Способ для производства биологических препаратов на основе энтомопатогенных нематод, включающий пропитку инертного носителя гомогенатом компонентов питательной среды, автоклавирование, охлаждение и инокуляцию симбиотических бактерий и чистой культуры нематод в питательную среду, отличающийся тем, что автоклавирование питательной среды проводят в отдельной емкости, в которую затем инокулируют симбиотические бактерии, а после их размножения в эту же емкость инокулируют инвазионные личинки нематод. A method for the production of biological preparations based on entomopathogenic nematodes, including impregnation of an inert carrier with a homogenate of nutrient medium components, autoclaving, cooling and inoculation of symbiotic bacteria and a pure culture of nematodes into a nutrient medium, characterized in that the autoclaving of the nutrient medium is carried out in a separate container, into which it is then inoculated symbiotic bacteria, and after their reproduction, invasive nematode larvae are inoculated into the same container.

Claims (1)

Способ для производства биологических препаратов на основе энтомопатогенных нематод, включающий пропитку инертного носителя гомогенатом компонентов питательной среды, автоклавирование, охлаждение и инокуляцию симбиотических бактерий и чистой культуры нематод в питательную среду, отличающийся тем, что автоклавирование питательной среды проводят в отдельной емкости, в которую затем инокулируют симбиотические бактерии, а после их размножения в эту же емкость инокулируют инвазионные личинки нематод. A method for the production of biological preparations based on entomopathogenic nematodes, comprising impregnating an inert carrier with a homogenate of components of a nutrient medium, autoclaving, cooling and inoculating symbiotic bacteria and a pure culture of nematodes in a nutrient medium, characterized in that the autoclaving of the nutrient medium is carried out in a separate container, which is then inoculated symbiotic bacteria, and after their propagation, invasive nematode larvae are inoculated into the same container.
RU2009145253/10A 2009-12-07 2009-12-07 Method of producing entomopathogenic nematode-based biological agents RU2444195C2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU2009145253/10A RU2444195C2 (en) 2009-12-07 2009-12-07 Method of producing entomopathogenic nematode-based biological agents

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Application Number Priority Date Filing Date Title
RU2009145253/10A RU2444195C2 (en) 2009-12-07 2009-12-07 Method of producing entomopathogenic nematode-based biological agents

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RU2009145253A true RU2009145253A (en) 2011-06-20
RU2444195C2 RU2444195C2 (en) 2012-03-10

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RU2009145253/10A RU2444195C2 (en) 2009-12-07 2009-12-07 Method of producing entomopathogenic nematode-based biological agents

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2748023C1 (en) * 2020-11-12 2021-05-19 Федеральное государственное бюджетное научное учреждение «Всероссийский научно-исследовательский институт защиты растений» Method for obtaining mass culture of symbiotic bacteria xenorhabdus of entomopathogenic nematodes rhabditida: steinernematidae

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Effective date: 20161208

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Effective date: 20200122