RU2008152363A - METHOD OF CULTIVATION OF LIPASE PRODUCT - Google Patents
METHOD OF CULTIVATION OF LIPASE PRODUCT Download PDFInfo
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- RU2008152363A RU2008152363A RU2008152363/10A RU2008152363A RU2008152363A RU 2008152363 A RU2008152363 A RU 2008152363A RU 2008152363/10 A RU2008152363/10 A RU 2008152363/10A RU 2008152363 A RU2008152363 A RU 2008152363A RU 2008152363 A RU2008152363 A RU 2008152363A
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- cultivation
- cultivating
- lipase
- hours
- nutrient medium
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Abstract
Способ культивирования продуцента липазы, включающий подготовку питательной среды, инокуляцию ее водно-споровой суспензией продуцента липазы, его глубинное культивирование, фильтрацию культуральной жидкости, новым является то, что в качестве продуцента липазы используют Rhizopus oryzae 1403, а при подготовке питательной среды в нее вносят, мас.%: рыбную муку - 3,0-3,5; подсолнечное масло - 0,2-0,3; кукурузный экстракт - 0,8-0,9; гидрофосфат аммония - 0,4-0,5; вода - остальное; культивирование осуществляют при аэрации и температуре 30-32°С в течение 48 ч, после чего в культуральную среду вносят тритон Х-305 в количестве 0,008-0,010 мас.% и продолжают культивирование еще 24 ч. A method of cultivating a lipase producer, including preparing a nutrient medium, inoculating it with an aqueous-spore suspension of a lipase producer, cultivating it deeply, filtering the culture fluid, new is that Rhizopus oryzae 1403 is used as a lipase producer, and is introduced into the preparation of the nutrient medium, wt.%: fish meal - 3.0-3.5; sunflower oil - 0.2-0.3; corn extract - 0.8-0.9; ammonium hydrogen phosphate - 0.4-0.5; water - the rest; cultivation is carried out at aeration and a temperature of 30-32 ° C for 48 hours, after which Triton X-305 is added to the culture medium in an amount of 0.008-0.010 wt.% and cultivation is continued for another 24 hours
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Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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RU2008152363/10A RU2397247C1 (en) | 2008-12-29 | 2008-12-29 | Lipase biosynthesis method |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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RU2008152363/10A RU2397247C1 (en) | 2008-12-29 | 2008-12-29 | Lipase biosynthesis method |
Publications (2)
Publication Number | Publication Date |
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RU2008152363A true RU2008152363A (en) | 2010-07-10 |
RU2397247C1 RU2397247C1 (en) | 2010-08-20 |
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RU2008152363/10A RU2397247C1 (en) | 2008-12-29 | 2008-12-29 | Lipase biosynthesis method |
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RU (1) | RU2397247C1 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108751427A (en) * | 2016-02-29 | 2018-11-06 | 常州爱上学教育科技有限公司 | Handle the preparation method of heavy metal wastewater thereby Rhizopus oryzae pompon |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
RU2549681C1 (en) * | 2014-07-25 | 2015-04-27 | Государственное научное учреждение научно-исследовательский институт кондитерской промышленности Российской академии сельскохозяйственных наук (ГНУ НИИКП Россельхозакадемии) | Nutrient medium for determination of lipase-producing microorganisms in confectionary products |
RU2566561C1 (en) * | 2014-07-25 | 2015-10-27 | Государственное научное учреждение научно-исследовательский институт кондитерской промышленности Российской академии сельскохозяйственных наук (ГНУ НИИКП Россельхозакадемии) | Method of detecting lipase-producing microorganisms in confectionary products |
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2008
- 2008-12-29 RU RU2008152363/10A patent/RU2397247C1/en not_active IP Right Cessation
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108751427A (en) * | 2016-02-29 | 2018-11-06 | 常州爱上学教育科技有限公司 | Handle the preparation method of heavy metal wastewater thereby Rhizopus oryzae pompon |
CN108751427B (en) * | 2016-02-29 | 2021-01-29 | 微力生物科技(威海)有限责任公司 | Preparation method of rhizopus oryzae mycelium pellets for treating heavy metal wastewater |
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Publication number | Publication date |
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RU2397247C1 (en) | 2010-08-20 |
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MM4A | The patent is invalid due to non-payment of fees |
Effective date: 20101230 |